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Genetic interaction mapping in mammalian cells using CRISPR interference
Open accessAbstract
We describe a combinatorial CRISPR interference (CRISPRi) screening platform for mapping genetic interactions in mammalian cells. We targeted 107 chromatin-regulation factors in human cells with pools of either single or double single guide RNAs (sgRNAs) to downregulate individual genes or gene pairs, respectively. Relative enrichment analysis of individual sgRNAs or sgRNA pairs allowed for quantitative characterization of genetic interactions, and comparison with protein–protein-interaction data revealed a functional map of chromatin regulation.
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Cites
CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes
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Self-citationGenome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
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Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation
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Self-citationA global genetic interaction network maps a wiring diagram of cellular function
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Self-citationCRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs
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Self-citationSystematic interpretation of genetic interactions using protein networks
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Self-citationConservation and Rewiring of Functional Modules Revealed by an Epistasis Map in Fission Yeast
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Exploring genetic interaction manifolds constructed from rich single-cell phenotypes
Norman, T. M., Horlbeck, M. A., Replogle, J. M., Ge, A. Y., Xu, A., Jost, M., Gilbert, L. A., & Weissman, J. S. (2019) · Science · doi:10.1126/science.aax4438 · Cited by 392
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Mapping the Genetic Landscape of Human Cells
Horlbeck, M. A., Xu, A., Wang, M., Bennett, N. K., Park, C. Y., Bogdanoff, D., Adamson, B., Chow, E. D., Kampmann, M., Peterson, T. R., et al. (2018) · Cell · doi:10.1016/j.cell.2018.06.010 · Cited by 319
CRISPR/Cas9-based engineering of the epigenome
Pulecio, J., Verma, N., Mejía-Ramírez, E., Huangfu, D., & Raya, A. (2017) · Cell Stem Cell · doi:10.1016/j.stem.2017.09.006 · Cited by 300
A new era in functional genomics screens
Przybyla, L., & Gilbert, L. A. (2022) · Nature Reviews Genetics · doi:10.1038/s41576-021-00409-w · Cited by 281
Global Genetic Networks and the Genotype-to-Phenotype Relationship
Costanzo, M., Kuzmin, E., van Leeuwen, J., Mair, B., Moffat, J., Boone, C., & Andrews, B. (2019) · Cell · doi:10.1016/j.cell.2019.01.033 · Cited by 241
Exosome-mediated delivery of gene vectors for gene therapy
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Prediction of the sequence-specific cleavage activity of Cas9 variants
Kim, N., Kim, H. K., Lee, S., Seo, J. H., Choi, J. W., Park, J., Min, S., Yoon, S., Cho, S.-R., & Kim, H. H. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0537-9 · Cited by 198
A genome-wide atlas of co-essential modules assigns function to uncharacterized genes
Wainberg, M., Kamber, R. A., Balsubramani, A., Meyers, R. M., Sinnott-Armstrong, N., Hornburg, D., Jiang, L., Chan, J., Jian, R., Gu, M., et al. (2021) · Nature Genetics · doi:10.1038/s41588-021-00840-z · Cited by 204
Kim, H. K., Lee, S., Kim, Y., Park, J., Min, S., Choi, J. W., Huang, T. P., Yoon, S., Liu, D. R., & Kim, H. H. (2020) · Nature Biomedical Engineering · doi:10.1038/s41551-019-0505-1 · Cited by 145
Genetic interaction mapping informs integrative structure determination of protein complexes
Braberg, H., Echeverria, I., Bohn, S., Cimermancic, P., Shiver, A., Alexander, R., Xu, J., Shales, M., Dronamraju, R., Jiang, S., et al. (2020) · Science · doi:10.1126/science.aaz4910 · Cited by 37
Self-citationDirect capture of CRISPR guides enables scalable, multiplexed, and multi-omic Perturb-seq
Replogle, J. M., Xu, A., Norman, T. M., Meer, E. J., Terry, J. M., Riordan, D., Srinivas, N., Mikkelsen, T. S., Weissman, J. S., & Adamson, B. (2018) · doi:10.1101/503367 · Cited by 465
Metadata
- Authors
- Du, D., Roguev, A., Gordon, D. E., Chen, M., Chen, S.-H., Shales, M., Shen, J. P., Ideker, T., Mali, P., Qi, L. S., & Krogan, N. J.
- Venue
- Nature Methods
- Published
- 2017-05-08
- Volume
- 14
- Issue
- 6
- Pages
- 577-580
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2614103706
- Cited by
- 163
- References
- 8
- FWCI
- 8.25
- Type
- journal article
- Language
- en
- Open access
- green
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