The DOI matches its Crossref record; 69 of the 69 references it cites were found in a registry or an index.
Tracking epitope-specific T cells
Open accessAbstract
The tracking of antigen-specific T cells in vivo is a useful approach for the study of the adaptive immune response. This protocol describes how populations of T cells specific for a given peptide–major histocompatibility complex (pMHC) epitope can be tracked based solely on T-cell receptor (TCR) specificity as opposed to other indirect methods based on function. The methodology involves the adoptive transfer of TCR transgenic T cells with defined epitope specificity into histocompatible mice and the subsequent detection of these cells through the use of congenic or clonotypic markers. Alternatively, endogenous epitope-specific T cells can be tracked directly through the use of pMHC tetramers. Using magnetic bead-based enrichment and advanced multiparameter flow cytometry, populations as small as five epitope-specific T cells can be detected from the peripheral lymphoid organs of a mouse. The adoptive transfer procedure can be completed within 3 h, whereas analysis of epitope-specific cells from mice can be completed within 6 h.
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Cites
Phenotypic Analysis of Antigen-Specific T Lymphocytes
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“pMHC tetramers are comprised of the actual ligands recognized by the TCRs of epitope-specific T cells, so in theory they should bind all epitope-specific T cells within a tissue sampl”
BackgroundRAG-1-deficient mice have no mature B and T lymphocytes
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RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement
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Counting Antigen-Specific CD8 T Cells: A Reevaluation of Bystander Activation during Viral Infection
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Induction by Antigen of Intrathymic Apoptosis of CD4 + CD8 + TCR lo Thymocytes in Vivo
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“These applications may include experiments in which large numbers of epitope-specific cells are needed for biochemical assays, or experiments in which a high frequency of epitope-specific cells in lymphoid tissues are needed to ensure visualization during immunohistochemica”
BackgroundSelf-citationMoon, J. J., Chu, H. H., Pepper, M., McSorley, S. J., Jameson, S. C., Kedl, R. M., & Jenkins, M. K. (2007) · Immunity · doi:10.1016/j.immuni.2007.07.007 · Cited by 976
Self-citationVisualization of peptide-specific T cell immunity and peripheral tolerance induction in vivo
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Cited by
CEACAM1 regulates TIM-3-mediated tolerance and exhaustion
Huang, Y.-H., Zhu, C., Kondo, Y., Anderson, A. C., Gandhi, A., Russell, A., Dougan, S. K., Petersen, B.-S., Melum, E., Pertel, T., et al. (2015) · Nature · doi:10.1038/nature13848 · Cited by 717
Wing, J. B., Ise, W., Kurosaki, T., & Sakaguchi, S. (2014) · Immunity · doi:10.1016/j.immuni.2014.12.006 · Cited by 388
Künzli, M., & Masopust, D. (2023) · Nature Immunology · doi:10.1038/s41590-023-01510-4 · Cited by 338
Interleukin-2-Dependent Allergen-Specific Tissue-Resident Memory Cells Drive Asthma
Hondowicz, B. D., An, D., Schenkel, J. M., Kim, K. S., Steach, H. R., Krishnamurty, A. T., Keitany, G. J., Garza, E. N., Fraser, K. A., Moon, J. J., et al. (2016) · Immunity · doi:10.1016/j.immuni.2015.11.004 · Cited by 293
Self-citationWiesner, D. L., Specht, C. A., Lee, C. K., Smith, K. D., Mukaremera, L., Lee, S. T., Lee, C. G., Elias, J. A., Nielsen, J. N., Boulware, D. R., et al. (2015) · PLOS Pathogens · doi:10.1371/journal.ppat.1004701 · Cited by 214
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Metadata
- Authors
- Moon, J. J., Chu, H. H., Hataye, J., Pagán, A. J., Pepper, M., McLachlan, J. B., Zell, T., & Jenkins, M. K.
- Venue
- Nature Protocols
- Published
- 2009-04
- Volume
- 4
- Issue
- 4
- Pages
- 565-581
- ISSN
- 1750-2799 (ISSN-L)
- 1754-2189
- OpenAlex
- W2047366270
- Cited by
- 288
- References
- 69
- FWCI
- 6.24
- Type
- journal article
- Language
- en
- Open access
- green
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