Cited by 380RNA Interference and Gene DeliveryCRISPR and Genetic EngineeringRNA Research and Splicing

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High-throughput RNAi screening in cultured cells: a user's guide

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Echeverri, Christophe J., Perrimon, Norbert·2006·Nature Reviews Genetics

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Abstract

RNA interference has re-energized the field of functional genomics by enabling genome-scale loss-of-function screens in cultured cells. Looking back on the lessons that have been learned from the first wave of technology developments and applications in this exciting field, we provide both a user's guide for newcomers to the field and a detailed examination of some more complex issues, particularly concerning optimization and quality control, for more advanced users. From a discussion of cell lines, screening paradigms, reagent types and read-out methodologies, we explore in particular the complexities of designing optimal controls and normalization strategies for these challenging but extremely powerful studies.

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Authors: Echeverri, C. J., & Perrimon, N.
Affiliations: Cenix BioScience (Germany) (DE)
Howard Hughes Medical Institute (US)
ORCID: Perrimon, Norbert
Venue: Nature Reviews Genetics
Publisher: Springer Science and Business Media LLC
Published: 2006-04-11
Volume: 7
Issue: 5
Pages: 373-384
ISSN: 1471-0056 (ISSN-L)
1471-0064
DOI: 10.1038/nrg1836
OpenAlex: W1968410036
Cited by: 380
References: 78
Citations by year: 2026: 4 · 2025: 6 · 2024: 12 · 2023: 7 · 2022: 7 · 2021: 10 · 2020: 15 · 2019: 10 · 2018: 13 · 2017: 14 · 2016: 18 · 2015: 17 · 2014: 25 · 2013: 20 · 2012: 23
FWCI: 3.08
Type: journal article
Language: en
Open access: green
nrs.harvard.edu
Licence: cc-by
www.springer.com/tdm (tdm)
Locations: Nature Reviews Genetics (publishedVersion)
PubMed (publishedVersion)
Digital Access to Scholarship at Harvard (DASH) (Harvard University) (submittedVersion, cc-by)
Funders: Howard Hughes Medical Institute
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
Keywords: RNA interference, high-throughput screening, loss-of-function screen, functional genomics, cell lines, screening optimization, quality control, normalization strategies, control system design
MeSH terms: Animals, Automation, Cells, Cultured, Drosophila melanogaster, Humans, Models, Biological, Phenotype, Quality Control, Gene Transfer Techniques, Gene Expression Profiling, RNA Interference, RNA, Small Interfering

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High-throughput RNAi screening in cultured cells: a user's guide

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