Cited by 297Microtubule and mitosis dynamicsProtein Structure and DynamicsCancer-related Molecular Pathways

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The Mad2 spindle checkpoint protein has two distinct natively folded states

Luo, Xuelian, Tang, Zhanyun, Xia, Guohong, Wassmann, Katja, Matsumoto, Tomohiro, Rizo, Josep, Yu, Hongtao·2004·Nature Structural & Molecular Biology

DOI 10.1038/nsmb748

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Abstract

The spindle checkpoint delays chromosome segregation in response to misaligned sister chromatids during mitosis, thus ensuring the fidelity of chromosome inheritance. Through binding to Cdc20, the Mad2 spindle checkpoint protein inhibits the target of this checkpoint, the ubiquitin protein ligase APC/C(Cdc20). We now show that without cofactor binding or covalent modification Mad2 adopts two distinct folded conformations at equilibrium (termed N1-Mad2 and N2-Mad2). The structure of N2-Mad2 has been determined by NMR spectroscopy. N2-Mad2 is much more potent in APC/C inhibition. Overexpression of a Mad2 mutant that specifically sequesters N2-Mad2 partially blocks checkpoint signaling in living cells. The two Mad2 conformers interconvert slowly in vitro, but interconversion is accelerated by a fragment of Mad1, an upstream regulator of Mad2. Our results suggest that the unusual two-state behavior of Mad2 is critical for spindle checkpoint signaling.

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Brünger, A. T., Adams, P. D., Clore, G. M., DeLano, W. L., Gros, P., Grosse‐Kunstleve, R. W., Jiang, J., Kuszewski, J. J., Nilges, M., Pannu, N., et al. (1998) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444998003254 · Cited by 15,723
MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures
Kraulis, P. J. (1991) · Journal of Applied Crystallography · doi:10.1107/s0021889891004399 · Cited by 14,011
Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
Elbashir, S. M., Harborth, J., Lendeckel, W., Yalcin, A., Weber, K., & Tuschl, T. (2001) · Nature · doi:10.1038/35078107 · Cited by 9,456
Prions
Prusiner, S. B. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.23.13363 · Cited by 5,373
[26] Raster3D: Photorealistic molecular graphics
Merritt, E. A., & Bacon, D. (1997) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(97)77028-9 · Cited by 3,749
Protein backbone angle restraints from searching a database for chemical shift and sequence homology
Cornilescu, G., Delaglio, F., & Bax, A. (1999) · Journal of Biomolecular NMR · doi:10.1023/a:1008392405740 · Cited by 2,957
Structure of influenza haemagglutinin at the pH of membrane fusion
Bullough, P. A., Hughson, F. M., Skehel, J. J., & Wiley, D. C. (1994) · Nature · doi:10.1038/371037a0 · Cited by 1,564
Program DYNAFIT for the Analysis of Enzyme Kinetic Data: Application to HIV Proteinase
Kuzmič, P. (1996) · Analytical Biochemistry · doi:10.1006/abio.1996.0238 · Cited by 1,494
Centromeres and Kinetochores
Cleveland, D. W., Mao, Y., & Sullivan, K. F. (2003) · Cell · doi:10.1016/s0092-8674(03)00115-6 · Cited by 1,003
Checkpoint inhibition of the APC/C in HeLa cells is mediated by a complex of BUBR1, BUB3, CDC20, and MAD2
Sudakin, V., Chan, G. K. T., & Yen, T. J. (2001) · The Journal of Cell Biology · doi:10.1083/jcb.200102093 · Cited by 927

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Sikosek, T., & Chan, H. S. (2014) · Journal of The Royal Society Interface · doi:10.1098/rsif.2014.0419 · Cited by 231
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Bub1 mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis
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Kinetochore structure and function.
Chan, G., Liu, S.-T., & Yen, T. (2005) · Trends in Cell Biology · doi:10.1016/j.tcb.2005.09.010 · Cited by 238
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Removal of Spindly from microtubule-attached kinetochores controls spindle checkpoint silencing in human cellsPreprint
Gassmann, R., Holland, A. J., Varma, D., Wan, X., Çivril, F., Cleveland Don, W., Oegema, K., Salmon, E. D., & Desai, A. (2010) · Genes & Development · doi:10.1101/gad.1886810 · Cited by 194
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Bryan, P. N., & Orban, J. P. (2010) · Current Opinion in Structural Biology · doi:10.1016/j.sbi.2010.06.002 · Cited by 192
p31comet Blocks Mad2 Activation through Structural Mimicry
Yang, M., Li, B., Tomchick, D. R., Machius, M., Rizo, J., Yu, H., & Luo, X. (2007) · Cell · doi:10.1016/j.cell.2007.08.048 · Cited by 197
Self-citation
Joined at the hip: kinetochores, microtubules, and spindle assembly checkpoint signaling
Sacristán, C., & Kops, G. J. P. L. (2014) · Trends in Cell Biology · doi:10.1016/j.tcb.2014.08.006 · Cited by 182
Fibrils with parallel in-register structure constitute a major class of amyloid fibrils: molecular insights from electron paramagnetic resonance spectroscopy
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Ha, J., & Loh, S. N. (2012) · Chemistry - A European Journal · doi:10.1002/chem.201200348 · Cited by 168
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Self-citation
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Self-citation
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Self-citation
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Self-citation
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Self-citation
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Self-citation
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Ye, Q., Kim, D. H., Dereli, I., Rosenberg, S., Hagemann, G., Herzog, F., Tóth, A., Cleveland, D. W., & Corbett, K. D. (2017) · The EMBO Journal · doi:10.15252/embj.201797291 · Cited by 91
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Authors: Luo, X., Tang, Z., Xia, G., Wassmann, K., Matsumoto, T., Rizo, J., & Yu, H.
Affiliations: The University of Texas Southwestern Medical Center (US)
Centre National de la Recherche Scientifique (FR)
Kyoto University (JP)
Corresponding author: Rizo, Josep
Yu, Hongtao
ORCID: Luo, Xuelian
Wassmann, Katja
Matsumoto, Tomohiro
Rizo, Josep
Yu, Hongtao
Venue: Nature Structural & Molecular Biology
Publisher: Springer Science and Business Media LLC
Published: 2004-03-14
Volume: 11
Issue: 4
Pages: 338-345
ISSN: 1545-9985 (ISSN-L)
1545-9993
DOI: 10.1038/nsmb748
OpenAlex: W2112512547
Cited by: 297
References: 42
Citations by year: 2026: 4 · 2025: 11 · 2024: 10 · 2023: 16 · 2022: 14 · 2021: 11 · 2020: 12 · 2019: 6 · 2018: 18 · 2017: 15 · 2016: 16 · 2015: 17 · 2014: 17 · 2013: 15 · 2012: 9
FWCI: 5.67
Type: journal article
Language: en
Open access: closed
Licence: www.springer.com/tdm (tdm)
Locations: Nature Structural & Molecular Biology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
Keywords: Mad2, spindle assembly checkpoint, conformational equilibrium, protein folding, CDC20, Mad1, mitosis, chromosome segregation fidelity, NMR structure
MeSH terms: Calcium-Binding Proteins, Cloning, Molecular, HeLa Cells, Humans, Spindle Apparatus, Magnetic Resonance Spectroscopy, Protein Conformation, Recombinant Proteins, Repressor Proteins, Transfection, Restriction Mapping, Cyclins, Protein Folding, Cell Cycle Proteins, Cdc20 Proteins, Mad2 Proteins

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The Mad2 spindle checkpoint protein has two distinct natively folded states

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