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Cited by 49Muscle metabolism and nutritionNutrition and Health in AgingMuscle Physiology and Disorders

Effect of weight loss on the rate of muscle protein synthesis during fasted and fed conditions in obese older adults

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Villareal, Dennis T., Smith, Gordon I., Shah, Krupa, Mittendorfer, Bettina·2012·Obesity
DOI 10.1038/oby.2011.280PMC PMC3291735
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Abstract

Although weight loss ameliorates many of the metabolic abnormalities associated with obesity, there has been reluctance to prescribe weight loss in obese, older individuals because of the fear that it will cause debilitating loss of muscle mass and impair physical function. To gain insight into the mechanisms responsible for the weight loss‐induced changes in muscle mass, we measured the rate of muscle protein synthesis (by using stable isotope labeled tracer methodology) during basal, postabsorptive conditions and during mixed meal ingestion in eight obese, older adults: (i) before weight loss therapy, (ii) ∼3 months after starting the weight loss intervention (i.e., during the active weight loss phase), when subjects had lost ∼7% of their initial body weight, and (iii) after they had lost ∼10% of their body weight and maintained this new body weight for ∼6 months (∼12 months after starting the weight loss intervention). The basal muscle protein fractional synthesis rate (FSR) was not affected by weight loss. Mixed meal ingestion stimulated the rate of muscle protein synthesis, and the anabolic response (i.e., increase in the protein synthesis rate above basal values) was greater (P < 0.05) during negative energy balance and active weight loss at 3 months (0.033 ± 0.012%·per hour, mean ± s.e.m.) than during weight maintenance before and at 12 months of weight loss therapy (0.003 ± 0.003 and 0.008 ± 0.012%·per hour, respectively). We conclude that during dietary calorie restriction and weight loss in older adults, the rate of muscle protein synthesis is not impaired. Thus, the loss of muscle mass must be mediated predominately by adverse effects of dietary calorie restriction on muscle proteolysis.

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  • Prolonged moderate calorie restriction and 5-10% weight loss increased the rate of muscle protein synthesis.

    “Prolonged moderate calorie restriction and 5-10% weight loss, on the other hand, increased the rate of muscle protein synthesis (78,79).”

    From Preserving Healthy Muscle during Weight Loss (2017) · cites this paper as [79]

    The Abstract reports a greater muscle protein synthesis response to feeding during active calorie restriction at about 7% weight loss. It also says basal synthesis was unchanged, and the enhanced feeding response was not seen after about 10% weight loss followed by weight maintenance. The claim omits these conditions and makes the increase sound general across 5–10% weight loss. (LLM, conf=0.90)

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  • Prolonged reduction of caloric intake, leading to 5%-10% weight loss, increases the rate of muscle protein synthesis, suggesting that muscle mass loss during prolonged moderate calorie restriction is mediated by increased muscle proteolysis rather than suppressed muscle protein synthesis.

    “prolonged reduction of caloric intake, leading to the common target of 5%-10% weight loss, increases the rate of muscle protein synthesis, 13,14 suggesting that muscle mass loss during prolonged moderate calorie restriction is mediated by increased muscle proteolysis rather than suppressed muscle protein synthesis.”

    From Changes in lean body mass with <scp>glucagon‐like peptide</scp> ‐1‐based therapies and mitigation strategies (2024) · cites this paper as [14]

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Authors
Villareal, D. T., Smith, G. I., Shah, K., & Mittendorfer, B.
Affiliations
  • University of New Mexico (US)
  • Washington University in St. Louis (US)
Corresponding author
Villareal, Dennis T.
ORCID
Venue
Obesity
Publisher
Wiley
Published
2012-09
Volume
20
Issue
9
Pages
1780-1786
ISSN
  • 1930-7381 (ISSN-L)
  • 1930-739X
DOI
10.1038/oby.2011.280
PMC
PMC3291735
OpenAlex
W2023701379
Semantic Scholar
efe165c22da6077c97e9c1fd720fea411b3d6a89
Cited by
49
References
30
Citations by year
2026: 3 · 2025: 1 · 2024: 7 · 2023: 2 · 2022: 2 · 2021: 2 · 2020: 3 · 2019: 3 · 2018: 3 · 2017: 1 · 2016: 2 · 2015: 3 · 2014: 8 · 2013: 5 · 2012: 4
FWCI
2.61
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor, 46 d)
Locations
  • Obesity (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Institutes of Health (UL1TR000041, UL1TR001449, 5p41rr000954-34, 7r01ag025501-06)
  • National Institute on Aging (R01-AG025501, K07AG021164, AG 021164, R01AG031176, AG-025501)
  • National Institute of General Medical Sciences (P41GM103422)
  • National Institute of Diabetes and Digestive and Kidney Diseases (DK56341, P30DK056341)
  • National Institute of Arthritis and Musculoskeletal and Skin Diseases (R01AR049869, AR 049869)
  • National Center for Research Resources (P41-RR000954, RR 00954, RR.00036, M01RR000036)
  • National Center for Advancing Translational Sciences
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
  • Health Sciences › Medicine › Physiology › Nutrition and Health in Aging
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
Keywords
weight loss, muscle protein synthesis, older adults, caloric restriction, negative energy balance, postabsorptive state, fractional synthesis rate, muscle mass loss, muscle protein degradation, weight maintenance
MeSH terms
Aged, Aged, 80 and over, Aging, Blood Glucose, Energy Intake, Energy Metabolism, Fasting, Female, Humans, Insulin, Leucine, Male, Muscle Proteins, Obesity, Protein Biosynthesis, Weight Loss, Muscle, Skeletal, Muscle Strength

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