The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.
Indole-3-lactic acid, a metabolite of tryptophan, secreted by Bifidobacterium longum subspecies infantis is anti-inflammatory in the immature intestine
Open accessAbstract
Necrotizing enterocolitis (NEC), a necrotic inflammation of the intestine, represents a major health problem in the very premature infant. Although prevention is difficult, the combination of ingestion of maternal-expressed breastmilk in conjunction with a probiotic provides the best protection. In this study, we establish a mechanism for breastmilk/probiotic protection. Ultra-high-performance liquid chromatography-tandem mass spectrometry of Bifidobacterium longum subsp. infantis (B. infantis) secretions was used to identify an anti-inflammatory molecule. Indole-3-lactic acid (ILA) was then tested in an established human immature small intestinal cell line, necrotizing colitis enterocytes, and other immature human enteroids for anti-inflammatory effects and to establish developmental function. ILA was also examined in immature and mature enterocytes. We have identified ILA, a metabolite of breastmilk tryptophan, as the anti-inflammatory molecule. This molecule is developmentally functional in immature but not mature intestinal enterocytes; ILA reduces the interleukin-8 (IL-8) response after IL-1β stimulus. It interacts with the transcription factor aryl hydrocarbon receptor (AHR) and prevents transcription of the inflammatory cytokine IL-8. This molecule produced by B. infantis (ATCC No. 15697) interaction with ingested breastmilk functions in a complementary manner and could become useful in the treatment of all at-risk premature infants for NEC if safety and clinical studies are performed.
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Cites
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Indole and Tryptophan Metabolism: Endogenous and Dietary Routes to Ah Receptor Activation
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Cited by
Probiotics, prebiotics, and postbiotics in health and disease
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Ecological and molecular perspectives on responders and non-responders to probiotics and prebiotics
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Shiver, A. L., Sun, J., Culver, R., Violette, A., Wynter, C., Nieckarz, M., Mattiello, S. P., Sekhon, P. K., Bottacini, F., Friess, L., et al. (2025) · Cell · doi:10.1016/j.cell.2025.02.010 · Cited by 27
Metadata
- Authors
- Meng, D., Sommella, E., Salviati, E., Campiglia, P., Ganguli, K., Djebali, K., Zhu, W., & Walker, W. A.
- Venue
- Pediatric Research
- Published
- 2020-01-16
- Volume
- 88
- Issue
- 2
- Pages
- 209-217
- ISSN
- 0031-3998 (ISSN-L)
- 1530-0447
- OpenAlex
- W2999597222
- Cited by
- 334
- References
- 39
- FWCI
- 19.06
- Type
- journal article
- Language
- en
- Open access
- green
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- Meng, D., Sommella, E., Salviati, E., Campiglia, P., Ganguli, K., Djebali, K., Zhu, W., & Walker, W. A. (2020). Indole-3-lactic acid, a metabolite of tryptophan, secreted by Bifidobacterium longum subspecies infantis is anti-inflammatory in the immature intestine. Pediatric Research. https://doi.org/10.1038/s41390-019-0740-x
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