The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.
Single cell analysis of kynurenine and System L amino acid transport in T cells
Open accessAbstract
The tryptophan metabolite kynurenine has critical immunomodulatory properties and can function as an aryl hydrocarbon receptor (AHR) ligand. Here we show that the ability of T cells to transport kynurenine is restricted to cells activated by the T-cell antigen receptor or proinflammatory cytokines. Kynurenine is transported across the T-cell membrane by the System L transporter SLC7A5. Accordingly, the ability of kynurenine to activate the AHR is restricted to T cells that express SLC7A5. We use the fluorescence spectral properties of kynurenine to develop a flow cytometry-based assay for rapid, sensitive and quantitative measurement of the kynurenine transport capacity in a single cell. Our findings provide a method to assess the susceptibility of T cells to kynurenine, and a sensitive single cell assay to monitor System L amino acid transport.
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Citations
Cites
Ido expression by dendritic cells: tolerance and tryptophan catabolism
Mellor, A. L., & Munn, D. H. (2004) · Nature Reviews Immunology · doi:10.1038/nri1457 · Cited by 2,341
The transcription factor Myc controls metabolic reprogramming upon T lymphocyte activation
Wang, R., Dillon, C. P., Shi, L. Z., Milasta, S., Carter, R., Finkelstein, D., McCormick, L. L., Fitzgerald, P., Chi, H., Munger, J., et al. (2011) · Immunity · doi:10.1016/j.immuni.2011.09.021 · Cited by 2,140 · cited 1×
“SLC7A5-mediated amino acid transport is also critical for maintaining expression of the transcription factor c-Myc, a key regulator of lymphocyte metabolism 25, 28 .”
An endogenous tumour-promoting ligand of the human aryl hydrocarbon receptor
Opitz, C. A., Litzenburger, U. M., Sahm, F., Ott, M., Tritschler, I., Trump, S., Schumacher, T., Jestaedt, L., Schrenk, D., Weller, M., et al. (2011) · Nature · doi:10.1038/nature10491 · Cited by 1,898
Control of Treg and TH17 cell differentiation by the aryl hydrocarbon receptor
Quintana, F. J., Basso, A. S., Iglesias, A. H., Korn, T., Farez, M. F., Bettelli, E., Caccamo, M., Oukka, M., & Weiner, H. L. (2008) · Nature · doi:10.1038/nature06880 · Cited by 1,865
The aryl hydrocarbon receptor links TH17-cell-mediated autoimmunity to environmental toxins
Veldhoen, M., Hirota, K., Westendorf, A. M., Buer, J., Dumoutier, L., Renauld, J.-C., & Stockinger, B. (2008) · Nature · doi:10.1038/nature06881 · Cited by 1,634
Phosphoenolpyruvate Is a Metabolic Checkpoint of Anti-tumor T Cell Responses
Ho, P.-C., Bihuniak, J. D., Macintyre, A. N., Staron, M., Liu, X., Amezquita, R., Tsui, Y.-C., Cui, G., Micevic, G., Perales, J. C., et al. (2015) · Cell · doi:10.1016/j.cell.2015.08.012 · Cited by 1,462
Indoleamine 2,3 dioxygenase and metabolic control of immune responses
Munn, D. H., & Mellor, A. L. (2013) · Trends in Immunology · doi:10.1016/j.it.2012.10.001 · Cited by 1,037
The aryl hydrocarbon receptor: multitasking in the immune system
Stockinger, B., Meglio, P. D., Gialitakis, M., & Duarte, J. H. (2014) · Annual Review of Immunology · doi:10.1146/annurev-immunol-032713-120245 · Cited by 909
Monitoring tryptophan metabolism in chronic immune activation
Schröcksnadel, K., Wirleitner, B., Winkler, C., & Fuchs, D. (2006) · Clinica Chimica Acta · doi:10.1016/j.cca.2005.06.013 · Cited by 551 · cited 2×
“Plasma levels of System L substrates range from 20 – 100 μ M and are relatively constant 29, 30 , whereas plasma levels of kynurenine are rarely above 3 μ M and subject to ef fi cient clearance 3, 31, 32 .”
The Aryl Hydrocarbon Receptor in Barrier Organ Physiology, Immunology, and Toxicology
Esser, C., & Rannug, A. (2015) · Pharmacological Reviews · doi:10.1124/pr.114.009001 · Cited by 501
Cited by
Immunometabolism and natural killer cell responses
O’Brien, K. L., & Finlay, D. K. (2019) · Nature Reviews Immunology · doi:10.1038/s41577-019-0139-2 · Cited by 435
Amino Acids and Their Transporters in T Cell Immunity and Cancer Therapy
Wang, W., & Zou, W. (2020) · Molecular Cell · doi:10.1016/j.molcel.2020.09.006 · Cited by 298
Quantitative analysis of T cell proteomes and environmental sensors during T cell differentiation
Howden, A. J. M., Hukelmann, J. L., Brenes, A., Spinelli, L., Sinclair, L. V., Lamond, A. I., & Cantrell, D. A. (2019) · Nature Immunology · doi:10.1038/s41590-019-0495-x · Cited by 231
Self-citationProtein synthesis, degradation, and energy metabolism in T cell immunity
Marchingo, J. M., & Cantrell, D. A. (2022) · Cellular & Molecular Immunology · doi:10.1038/s41423-021-00792-8 · Cited by 169
Self-citationMoffett, J. R., Arun, P., Puthillathu, N., Vengilote, R., Ives, J. A., Badawy, A. A.-B., & Namboodiri, A. M. (2020) · Frontiers in Immunology · doi:10.3389/fimmu.2020.00031 · Cited by 89
“SLC7A5 is also involved in kynurenine uptake and is expressed in T cells (95), B cells (96), and monocytes/macrophages (97), indicating that all of these cell types are able to take up extracellular kynurenine.”
Background
Metadata
- Authors
- Sinclair, L. V., Neyens, D., Ramsay, G., Taylor, P. M., & Cantrell, D. A.
- Venue
- Nature Communications
- Published
- 2018-05-17
- Volume
- 9
- Issue
- 1
- Pages
- 1981-1981
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2805022017
- Cited by
- 193
- References
- 11
- FWCI
- 3.78
- Type
- journal article
- Language
- en
- Open access
- gold
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