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Quantitative metaproteomics of medieval dental calculus reveals individual oral health status

Jersie-Christensen, Rosa R., Lanigan, Liam T., Lyon, David, Mackie, Meaghan, Belstrøm, Daniel, Kelstrup, Christian D., Fotakis, Anna K., Willerslev, Eske, Lynnerup, Niels, Jensen, Lars J., Cappellini, Enrico, Olsen, Jesper V.·2018·Nature Communications
DOI 10.1038/s41467-018-07148-3PMC PMC6246597
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Abstract

The composition of ancient oral microbiomes has recently become accessible owing to advanced biomolecular methods such as metagenomics and metaproteomics, but the utility of metaproteomics for such analyses is less explored. Here, we use quantitative metaproteomics to characterize the dental calculus associated with the remains of 21 humans retrieved during the archeological excavation of the medieval (ca. 1100–1450 CE) cemetery of Tjærby, Denmark. We identify 3671 protein groups, covering 220 bacterial species and 81 genera across all medieval samples. The metaproteome profiles of bacterial and human proteins suggest two distinct groups of archeological remains corresponding to health-predisposed and oral disease-susceptible individuals, which is supported by comparison to the calculus metaproteomes of healthy living individuals. Notably, the groupings identified by metaproteomics are not apparent from the bioarchaeological analysis, illustrating that quantitative metaproteomics has the potential to provide additional levels of molecular information about the oral health status of individuals from archeological contexts.

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  • Jersie-Christensen et al. identified two groups of individuals from medieval Denmark based on bacterial taxa: one showing evidence of microbiome dysbiosis and one showing a healthy microbiome.

    “On the basis of bacterial taxa, the study identified two groups of individuals: one showing evidence of microbiome dysbiosis (a disruption of a healthy state oral microbiome) and one group showing a healthy microbiome.”

    From Ancient protein analysis in archaeology (2021) · cites this paper as [61]

  • Most studies of ancient human remains work with historic dental calculus, a stable long-term reservoir of proteins.

    “When it comes to analyzing ancient human remains, most studies work with historic dental calculus [11, 15, 22-26], as it is a stable, long-term reservoir of proteins [27].”

    From Metaproteomic analysis of King Ghezo tomb wall (Abomey, Benin) confirms 19th century voodoo sacrifices (2024) · cites this paper as [25]

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Research works printed but not linked: 55

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Metadata

Authors
Jersie-Christensen, R. R., Lanigan, L. T., Lyon, D., Mackie, M., Belstrøm, D., Kelstrup, C. D., Fotakis, A. K., Willerslev, E., Lynnerup, N., Jensen, L. J., Cappellini, E., & Olsen, J. V.
ORCID
Venue
Nature Communications
Publisher
Springer Science and Business Media LLC
Published
2018-11-20
Volume
9
Issue
1
ISSN
2041-1723
DOI
10.1038/s41467-018-07148-3
PMC
PMC6246597
Cited by
100
References
9
Language
en
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