The DOI matches its Crossref record; 59 of the 59 references it cites were found in a registry or an index.
Marker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9
Open accessAbstract
Targeted insertion of transgenes at pre-determined plant genomic safe harbors provides a desirable alternative to insertions at random sites achieved through conventional methods. Most existing cases of targeted gene insertion in plants have either relied on the presence of a selectable marker gene in the insertion cassette or occurred at low frequency with relatively small DNA fragments (<1.8 kb). Here, we report the use of an optimized CRISPR-Cas9-based method to achieve the targeted insertion of a 5.2 kb carotenoid biosynthesis cassette at two genomic safe harbors in rice. We obtain marker-free rice plants with high carotenoid content in the seeds and no detectable penalty in morphology or yield. Whole-genome sequencing reveals the absence of off-target mutations by Cas9 in the engineered plants. These results demonstrate targeted gene insertion of marker-free DNA in rice using CRISPR-Cas9 genome editing, and offer a promising strategy for genetic improvement of rice and other crops.
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Directed evolution of hyperactive integrases for site specific insertion of transgenes
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Metadata
- Authors
- Dong, O. X., Yu, S., Jain, R., Zhang, N., Duong, P. Q., Butler, C., Li, Y., Lipzen, A., Martin, J. A., Barry, K. W., Schmutz, J., Tian, L., & Ronald, P. C.
- Venue
- Nature Communications
- Published
- 2020-03-04
- Volume
- 11
- Issue
- 1
- Pages
- 1178-1178
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W3009793704
- Cited by
- 348
- References
- 59
- FWCI
- 13.69
- Type
- journal article
- Language
- en
- Open access
- gold
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- Dong, O. X., Yu, S., Jain, R., Zhang, N., Duong, P. Q., Butler, C., Li, Y., Lipzen, A., Martin, J. A., Barry, K. W., Schmutz, J., Tian, L., & Ronald, P. C. (2020). Marker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9. Nature Communications. https://doi.org/10.1038/s41467-020-14981-y
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