Glucose-responsive insulin patch for the regulation of blood glucose in mice and minipigs
Open accessAbstract
Glucose-responsive insulin delivery systems that mimic pancreatic endocrine function could enhance health and improve quality of life for people with type 1 and type 2 diabetes with reduced β-cell function. However, insulin delivery systems with rapid in vivo glucose-responsive behaviour typically have limited insulin-loading capacities and cannot be manufactured easily. Here, we show that a single removable transdermal patch, bearing microneedles loaded with insulin and a non-degradable glucose-responsive polymeric matrix, and fabricated via in situ photopolymerization, regulated blood glucose in insulin-deficient diabetic mice and minipigs (for minipigs >25 kg, glucose regulation lasted >20 h with patches of ~5 cm 2). Under hyperglycaemic conditions, phenylboronic acid units within the polymeric matrix reversibly form glucose-boronate complexes that-owing to their increased negative charge-induce the swelling of the polymeric matrix and weaken the electrostatic interactions between the negatively charged insulin and polymers, promoting the rapid release of insulin. This proof-of-concept demonstration may aid the development of other translational stimuli-responsive microneedle patches for drug delivery.
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Another study used phenylboronic acid units and ISF glucose to trigger insulin release based on weakening of electrostatic interactions between insulin and microneedle matrix in a mice model.
“Similarly, another research study [165] used phenylboronic acid units and ISF glucose to trigger the insulin release based on the weakening of the electrostatic interactions between the insulin and microneedle matrix in a mice model.”
From The emergence of AI-based wearable sensors for digital health technology: a review (2023) · cites this paper as [165]
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Self-citation
- Authors
- Yu, J., Wang, J., Zhang, Y., Chen, G., Mao, W., Ye, Y., Kahkoska, A. R., Buse, J. B., Langer, R., & Gu, Z.
- Affiliations
- University of North Carolina at Chapel Hill (US)
- Corresponding author
- Gu, Zhen
- ORCID
- Venue
- Nature Biomedical Engineering
- Published
- 2020-02-03
- Volume
- 4
- Issue
- 5
- Pages
- 499-506
- ISSN
- 2157-846X (ISSN-L)
- PMC
- PMC7231631
- OpenAlex
- W3004097028
- Cited by
- 604
- References
- 50
- Citations by year
- 2026: 54 · 2025: 106 · 2024: 102 · 2023: 100 · 2022: 109 · 2021: 83 · 2020: 50
- FWCI
- 33.35
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- Locations
- Nature Biomedical Engineering (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- DSpace@MIT (Massachusetts Institute of Technology) (submittedVersion, cc-by-nc)
- eScholarship (California Digital Library) (submittedVersion)
- Funders
- UC | University of California, Los Angeles
- Topics
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advancements in Transdermal Drug Delivery
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes Management and Research
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Keywords
- glucose-responsive insulin delivery, transdermal patch, microneedles, phenylboronic acid, stimuli-responsive polymers, in situ photopolymerization, blood glucose regulation, diabetes mellitus, insulin secretion, insulin-deficient diabetes, minipigs
- MeSH terms
- Insulin Secretion, Animals, Blood Glucose, Diabetes Mellitus, Experimental, Disease Models, Animal, Humans, Insulin, Male, Mice, Inbred C57BL, Needles, Swine, Swine, Miniature, Drug Delivery Systems, Transdermal Patch
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