The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.
Microneedle patch for the ultrasensitive quantification of protein biomarkers in interstitial fluid
Open accessAbstract
The detection and quantification of protein biomarkers in interstitial fluid is hampered by challenges in its sampling and analysis. Here we report the use of a microneedle patch for fast in vivo sampling and on-needle quantification of target protein biomarkers in interstitial fluid. We used plasmonic fluor—an ultrabright fluorescent label—to improve the limit of detection of various interstitial fluid protein biomarkers by nearly 800-fold compared with conventional fluorophores, and a magnetic backing layer to implement conventional immunoassay procedures on the patch and thus improve measurement consistency. We used the microneedle patch in mice for minimally invasive evaluation of the efficiency of a cocaine vaccine, for longitudinal monitoring of the levels of inflammatory biomarkers, and for efficient sampling of the calvarial periosteum—a challenging site for biomarker detection—and the quantification of its levels of the matricellular protein periostin, which cannot be accurately inferred from blood or other systemic biofluids. Microneedle patches for the minimally invasive collection and analysis of biomarkers in interstitial fluid might facilitate point-of-care diagnostics and longitudinal monitoring. A microneedle patch that samples and quantifies target protein biomarkers in interstitial fluid allows for longitudinal monitoring of the levels of a range of disease-relevant biomarkers, as shown in live mice.
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Cites
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Gold Nanoparticles Quench Fluorescence by Phase Induced Radiative Rate Suppression
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Accessing analytes in biofluids for peripheral biochemical monitoring
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Self-citationSuction Blister Device for Separation of Viable Epidermis from Dermis*
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Mechanisms of sampling interstitial fluid from skin using a microneedle patch
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Sempionatto, J. R., Lasalde-Ramírez, J. A., Mahato, K., Wang, J., & Gao, W. (2022) · Nature Reviews Chemistry · doi:10.1038/s41570-022-00439-w · Cited by 613
SARS-CoV-2 breakthrough infections in vaccinated individuals: measurement, causes and impact
Lipsitch, M., Krammer, F., Regev-Yochay, G., Lustig, Y., & Balicer, R. D. (2022) · Nature Reviews Immunology · doi:10.1038/s41577-021-00662-4 · Cited by 336
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Lin, M., Hu, H., Zhou, S., & Xu, S. (2022) · Nature Reviews Materials · doi:10.1038/s41578-022-00427-y · Cited by 334
Lab under the Skin: Microneedle Based Wearable Devices
Teymourian, H., Tehrani, F., Mahato, K., & Wang, J. (2021) · Advanced Healthcare Materials · doi:10.1002/adhm.202002255 · Cited by 310
Georgescu, I., Dricu, A., Artene, S.-A., Vrăjitoru, N.-R., Barcan, E., Tache, D. E., Giubelan, L.-I., Staicu, G.-A., Manea (Carneluti), E.-V., Pană, C., et al. (2024) · Applied Sciences · doi:10.3390/app14188097 · Cited by 6
Metadata
- Authors
- Wang, Z., Luan, J., Seth, A., Liu, L., You, M., Gupta, P., Rathi, P., Wang, Y., Cao, S., Jiang, Q., Zhang, X., Gupta, R., Zhou, Q., Morrissey, J. J., Scheller, E. L., Rudra, J. S., & Singamaneni, S.
- Venue
- Nature Biomedical Engineering
- Published
- 2021-01-22
- Volume
- 5
- Issue
- 1
- Pages
- 64-76
- ISSN
- 2157-846X (ISSN-L)
- OpenAlex
- W3125908988
- Cited by
- 386
- References
- 45
- FWCI
- 20.60
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Wang, Z., Luan, J., Seth, A., Liu, L., You, M., Gupta, P., Rathi, P., Wang, Y., Cao, S., Jiang, Q., Zhang, X., Gupta, R., Zhou, Q., Morrissey, J. J., Scheller, E. L., Rudra, J. S., & Singamaneni, S. (2021). Microneedle patch for the ultrasensitive quantification of protein biomarkers in interstitial fluid. Nature Biomedical Engineering. https://doi.org/10.1038/s41551-020-00672-y
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