The DOI matches its Crossref record; 60 of the 60 references it cites were found in a registry or an index.
A lab-on-a-chip for the concurrent electrochemical detection of SARS-CoV-2 RNA and anti-SARS-CoV-2 antibodies in saliva and plasma
Open accessAbstract
Rapid, accurate and frequent detection of the RNA of SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) and of serological host antibodies to the virus would facilitate the determination of the immune status of individuals who have Coronavirus disease 2019 (COVID-19), were previously infected by the virus, or were vaccinated against the disease. Here we describe the development and application of a 3D-printed lab-on-a-chip that concurrently detects, via multiplexed electrochemical outputs and within 2 h, SARS-CoV-2 RNA in saliva as well as anti-SARS-CoV-2 immunoglobulins in saliva spiked with blood plasma. The device automatedly extracts, concentrates and amplifies SARS-CoV-2 RNA from unprocessed saliva, and integrates the Cas12a-based enzymatic detection of SARS-CoV-2 RNA via isothermal nucleic acid amplification with a sandwich-based enzyme-linked immunosorbent assay on electrodes functionalized with the Spike S1, nucleocapsid and receptor-binding-domain antigens of SARS-CoV-2. Inexpensive microfluidic electrochemical sensors for performing multiplexed diagnostics at the point of care may facilitate the widespread monitoring of COVID-19 infection and immunity. A 3D-printed lab-on-a-chip allows for the concurrent rapid electrochemical detection of SARS-CoV-2 RNA in saliva and of anti-SARS-CoV-2 antibodies in saliva spiked with blood plasma.
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Cites
Virological assessment of hospitalized patients with COVID-2019
Wölfel, R., Corman, V. M., Guggemos, W., Seilmaier, M., Zange, S., Müller, M. A., Niemeyer, D., Jones, T. C., Vollmar, P., Rothe, C., et al. (2020) · Nature · doi:10.1038/s41586-020-2196-x · Cited by 6,661 · cited 4×
“This platform incorporates multiple innovations including: (1) engineering of a high-specificity and high-sensitivity multiplexed EC sensor chip that enables detection of both proteins and nucleic acids with clinically relevant samples of biological fluids perfused through a single microfluidic channel; (2) developmen…”
MethodInfluentialSARS-CoV-2 Viral Load in Upper Respiratory Specimens of Infected Patients
Zou, L., Ruan, F., Huang, M., Liang, L., Huang, H., Hong, Z., Yu, J., Kang, M., Song, Y., Xia, J., et al. (2020) · New England Journal of Medicine · doi:10.1056/nejmc2001737 · Cited by 4,810
CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity
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Nucleic acid detection with CRISPR-Cas13a/C2c2
Gootenberg, J. S., Abudayyeh, O. O., Lee, J. W., Essletzbichler, P., Dy, A. J., Joung, J., Verdine, V., Donghia, N., Daringer, N. M., Freije, C. A., et al. (2017) · Science · doi:10.1126/science.aam9321 · Cited by 3,844
Self-citationAntibody responses to SARS-CoV-2 in patients with COVID-19
Long, Q.-X., Liu, B.-Z., Deng, H.-J., Wu, G.-C., Deng, K., Chen, Y.-K., Liao, P., Qiu, J.-F., Lin, Y., Cai, X.-F., et al. (2020) · Nature Medicine · doi:10.1038/s41591-020-0897-1 · Cited by 2,876
Self-citationCRISPR–Cas12-based detection of SARS-CoV-2
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Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6
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A serological assay to detect SARS-CoV-2 seroconversion in humans
Amanat, F., Stadlbauer, D., Strohmeier, S., Nguyen, T. H. O., Chromikova, V., McMahon, M., Jiang, K., Arunkumar, G. A., Jurczyszak, D., Polanco, J., et al. (2020) · Nature Medicine · doi:10.1038/s41591-020-0913-5 · Cited by 1,769
Evolution of antibody immunity to SARS-CoV-2
Gaebler, C., Wang, Z., Lorenzi, J. C. C., Muecksch, F., Finkin, S., Tokuyama, M., Cho, A., Jankovic, M., Schaefer-Babajew, D., Oliveira, T. Y., et al. (2021) · Nature · doi:10.1038/s41586-021-03207-w · Cited by 1,674
Cited by
Multiplex Cellular Microenvironment Detection Based on Surface-Enhanced Raman Scattering Patch
Wu, X., Zhang, Z., Zhu, F., Kuang, Y., Chen, S., Sun, C., Liu, B., & Wang, Y. (2025) · Chemistry–Methods · doi:10.1002/cmtd.202400083
Metadata
- Authors
- Najjar, D., Rainbow, J., Sharma Timilsina, S., Jolly, P., de Puig, H., Yafia, M., Durr, N., Sallum, H., Alter, G., Li, J. Z., Yu, X. G., Walt, D. R., Paradiso, J. A., Estrela, P., Collins, J. J., & Ingber, D. E.
- Venue
- Nature Biomedical Engineering
- Published
- 2022-08-08
- Volume
- 6
- Issue
- 8
- Pages
- 968-978
- ISSN
- 2157-846X (ISSN-L)
- OpenAlex
- W4290672784
- Cited by
- 291
- References
- 60
- FWCI
- 27.45
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Najjar, D., Rainbow, J., Sharma Timilsina, S., Jolly, P., de Puig, H., Yafia, M., Durr, N., Sallum, H., Alter, G., Li, J. Z., Yu, X. G., Walt, D. R., Paradiso, J. A., Estrela, P., Collins, J. J., & Ingber, D. E. (2022). A lab-on-a-chip for the concurrent electrochemical detection of SARS-CoV-2 RNA and anti-SARS-CoV-2 antibodies in saliva and plasma. Nature Biomedical Engineering. https://doi.org/10.1038/s41551-022-00919-w
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
