Giant light-harvesting nanoantenna for single-molecule detection in ambient light
Open accessAbstract
Here, we explore the enhancement of single molecule emission by polymeric nano-antenna that can harvest energy from thousands of donor dyes to a single acceptor. In this nano-antenna, the cationic dyes are brought together in very close proximity using bulky counterions, thus enabling ultrafast diffusion of excitation energy (≤30 fs) with minimal losses. Our 60-nm nanoparticles containing >10,000 rhodamine-based donor dyes can efficiently transfer energy to 1-2 acceptors resulting in an antenna effect of ~1,000. Therefore, single Cy5-based acceptors become 25-fold brighter than quantum dots QD655. This unprecedented amplification of the acceptor dye emission enables observation of single molecules at illumination powers (1-10 mW cm-2) that are >10,000-fold lower than typically required in single-molecule measurements. Finally, using a basic setup, which includes a 20X air objective and a sCMOS camera, we could detect single Cy5 molecules by simply shining divergent light on the sample at powers equivalent to sunlight.
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Such structures exhibit a giant light-harvesting behavior due to the efficient ultrafast excitation energy transfer (EET, <30 fs), enabling the detection of a single particle with ambient light.
“In addition, such structures exhibit a giant light-harvesting behavior due to the efficient ultrafast excitation energy transfer (EET, <30 fs), enabling the detection of a single particle with ambient light (Figure 8b).154”
From The Pursuit of Shortwave Infrared-Emitting Nanoparticles with Bright Fluorescence through Molecular Design and Excited-State Engineering of Molecular Aggregates (2022) · cites this paper as [154]
The Abstract and Discussion report giant light harvesting driven in part by EET on a ≤30 fs timescale. They describe detection of single Cy5 molecules using the nanoantenna under sunlight-equivalent illumination, rather than establishing the claim as phrased about detecting a single particle with ambient light. The Methods section on microscopy specifies that the illumination was an artificial white light source mimicking sunlight. (LLM, conf=0.84)
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“154 Using this approach, fluorescent polymer nanoparticles with varied fluorescence wavelengths have been developed (Figure 8c).”
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Self-citation
- Authors
- Trofymchuk, K., Reisch, A., Didier, P., Fras, F., Gilliot, P., Mely, Y., & Klymchenko, A. S.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Klymchenko, Andrey S.
- ORCID
- Venue
- Nature Photonics
- Published
- 2017-09-29
- Volume
- 11
- Issue
- 10
- Pages
- 657-663
- ISSN
- 1749-4885 (ISSN-L)
- 1749-4893
- PMC
- PMC5624503
- OpenAlex
- W2754836562
- Cited by
- 175
- References
- 55
- Citations by year
- 2026: 8 · 2025: 13 · 2024: 12 · 2023: 15 · 2022: 30 · 2021: 28 · 2020: 32 · 2019: 20 · 2018: 15 · 2017: 2
- FWCI
- 8.50
- Type
- journal article
- Language
- en
- Update notices
- correction (2018-01-12)
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Nature Photonics (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- HAL (submittedVersion)
- Funders
- Agence Nationale de la Recherche (ANR-10-INBS-0005)
- French Infrastructure for Integrated Structural Biology
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Plasmonic and Surface Plasmon Research
- Physical Sciences › Materials Science › Materials Chemistry › Luminescence and Fluorescent Materials
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Keywords
- single-molecule detection, single-molecule fluorescence, donor-acceptor energy transfer, polymeric nanoparticles, antenna effect, ambient light detection, single-molecule imaging
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