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Biofabrication strategies for 3D in vitro models and regenerative medicine
Open accessAbstract
Organs are complex systems composed of different cells, proteins and signalling molecules that are arranged in a highly ordered structure to orchestrate a myriad of functions in our body. Biofabrication strategies can be applied to engineer 3D tissue models in vitro by mimicking the structure and function of native tissue through the precise deposition and assembly of materials and cells. This approach allows the spatiotemporal control over cell–cell and cell–extracellular matrix communication and thus the recreation of tissue-like structures. In this Review, we examine biofabrication strategies for the construction of functional tissue replacements and organ models, focusing on the development of biomaterials, such as supramolecular and photosensitive materials, that can be processed using biofabrication techniques. We highlight bioprinted and bioassembled tissue models and survey biofabrication techniques for their potential to recreate complex tissue properties, such as shape, vasculature and specific functionalities. Finally, we discuss challenges, such as scalability and the foreign body response, and opportunities in the field and provide an outlook to the future of biofabrication in regenerative medicine. Biofabrication can be applied to replicate tissues and organs for regenerative medicine and for the creation of 3D in vitro tissue models. In this Review, the recent advances in biomaterials and biofabrication technologies are discussed, and challenges and opportunities are highlighted.
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“Commonly chosen materials include Matrigel, other natural biomaterials (e.g., gelatin, fi brin, collagen, alginate, chitosan, hyaluronic acid), and synthetic polymers (e.g., PEG, PCL) [40,87].”
From 3D In Vitro Model (R)evolution: Unveiling Tumor–Stroma Interactions (2021) · cites this paper as [40]
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“Common limitations, however, include slow printing speeds, development of nontoxic and printable bioinks, and insuf fi cient reproducibility to create standard models [38,40].”
From 3D In Vitro Model (R)evolution: Unveiling Tumor–Stroma Interactions (2021) · cites this paper as [40]
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“Over the past decades, multiple 3D bioprinting methods have been improved to allow the design of complex cell-laden 3D constructs.”
From 3D In Vitro Model (R)evolution: Unveiling Tumor–Stroma Interactions (2021) · cites this paper as [40]
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“Each of these technologies is application-speci fi c and the choice depends on the type of bioink used [40,87].”
From 3D In Vitro Model (R)evolution: Unveiling Tumor–Stroma Interactions (2021) · cites this paper as [40]
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“In laser-assisted bioprinting, a high-intensity laser is used to deposit less-viscous bioinks for cell patterning, thereby preserving high cell viability.”
From 3D In Vitro Model (R)evolution: Unveiling Tumor–Stroma Interactions (2021) · cites this paper as [40]
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“Despite its cell-compatibility, this is the most complex and expensive method of printing [40,90].”
From 3D In Vitro Model (R)evolution: Unveiling Tumor–Stroma Interactions (2021) · cites this paper as [40]
Experimental — accuracy not yet measuredIn the citing paper
“Biofabrication technologies, such as bioprinting, enable programmed assembly of cells into complex 3D geometries24,25.”
From 3D bioprinting of high cell-density heterogeneous tissue models through spheroid fusion within self-healing hydrogels (2021) · cites this paper as [25]
In the citing paper
“Various biofunctional materials and cell types can thus be printed simultaneously onto a substrate of cell-compatible biomaterials to build 3D complex constructs with good spatial resolution and reproducibility (Fig. 3a)130,148–151.”
From Tumor-on-a-chip: from bioinspired design to biomedical application (2021) · cites this paper as [148]
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Citations
Cites
Lutolf, M. P., & Hubbell, J. A. (2005) · Nature Biotechnology · doi:10.1038/nbt1055 · Cited by 4,551
Reconstituting organ-level lung functions on a chip
Huh, D., Matthews, B. D., Mammoto, A., Montoya-Zavala, M., Hsin, H. Y., & Ingber, D. E. (2010) · Science · doi:10.1126/science.1188302 · Cited by 4,250
Polymers for 3D Printing and Customized Additive Manufacturing
Ligon, S. C., Liska, R., Stampfl, J., Gurr, M., & Mülhaupt, R. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00074 · Cited by 3,942
Bhatia, S. N., & Ingber, D. E. (2014) · Nature Biotechnology · doi:10.1038/nbt.2989 · Cited by 3,305
Sydney Gladman, A., Matsumoto, E. A., Nuzzo, R. G., Mahadevan, L., & Lewis, J. A. (2016) · Nature Materials · doi:10.1038/nmat4544 · Cited by 3,027
Hydrogels as extracellular matrix mimics for 3D cell culture
Tibbitt, M. W., & Anseth, K. S. (2009) · Biotechnology and Bioengineering · doi:10.1002/bit.22361 · Cited by 2,727
A 3D bioprinting system to produce human-scale tissue constructs with structural integrity
Kang, H.-W., Lee, S. J., Ko, I. K., Kengla, C., Yoo, J. J., & Atala, A. (2016) · Nature Biotechnology · doi:10.1038/nbt.3413 · Cited by 2,622
Self-citationContinuous liquid interface production of 3D objects
Tumbleston, J. R., Shirvanyants, D. G., Ermoshkin, N., Janusziewicz, R., Johnson, A. R., Kelly, D., Chen, K., PINSCHMIDT, R. K. J., Rolland, J. P., Ermoshkin, A. V., et al. (2015) · Science · doi:10.1126/science.aaa2397 · Cited by 2,295
3D bioprinting of vascularized, heterogeneous cell-laden tissue constructs
Kolesky, D. B., Truby, R. L., Gladman, A. S., Busbee, T. A., Homan, K. A., & Lewis, J. A. (2014) · Advanced Materials · doi:10.1002/adma.201305506 · Cited by 2,102
Rapid casting of patterned vascular networks for perfusable engineered three-dimensional tissues
Miller, J. S., Stevens, K. R., Yang, M. T., Baker, B. M., Nguyen, D.-H. T., Cohen, D. M., Toro, E., Chen, A. A., Galie, P. A., Yu, X., et al. (2012) · Nature Materials · doi:10.1038/nmat3357 · Cited by 1,917
Research works printed but not linked: 2
- Levato, R. et al. The bio in the ink: cartilage regeneration with bioprintable hydrogels and articular cartilage-derived progenitor cells. Acta Biomaterialia (2017). · DOI
- Shi, W. et al. Structurally and functionally optimized silk-fibroin–gelatin scaffold using 3D printing to repair cartilage injury in vitro and in vivo. Adv. Mater. 29, 1701089 (2017). · DOI
Cited by
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Wu, S., Yang, T., Ma, M., Le, F., Ren, L., Liu, G., Wang, Y., Cheng, B., Xia, J., & Hao, Z. (2024) · Pharmacological Research · doi:10.1016/j.phrs.2024.107307 · Cited by 51
Guida, L., Cavallaro, M., & Levi, M. (2024) · Bioprinting · doi:10.1016/j.bprint.2024.e00376 · Cited by 52
Meininger, S., Moseke, C., Spatz, K., März, E., Blum, C., Ewald, A., & Vorndran, E. (2019) · Materials Science and Engineering C · doi:10.1016/j.msec.2019.01.053 · Cited by 49
A human-on-a-chip approach to tackling rare diseases
de Mello, C. P. P., Rumsey, J. W., Slaughter, V. L., & Hickman, J. J. (2019) · Drug Discovery Today · doi:10.1016/j.drudis.2019.08.001 · Cited by 49
3D Bioprinting tissue analogs: Current development and translational implications
Liu, S., Cheng, L., Liu, Y., Zhang, H., Song, Y., Park, J.-H., Dashnyam, K., Lee, J., Khalak, F. A.-H., Riester, O., et al. (2023) · Journal of Tissue Engineering · doi:10.1177/20417314231187113 · Cited by 51
Sound innovations for biofabrication and tissue engineering
Wu, M., Ma, Z., Tian, Z., Rich, J. T., He, X. B., Xia, J., He, Y., Yang, K., Yang, S., Leong, K. W., et al. (2024) · Microsystems & Nanoengineering · doi:10.1038/s41378-024-00759-5 · Cited by 53
Biofabrication for osteochondral tissue regeneration: bioink printability requirements
Abdulghani, S., & Morouço, P. (2019) · Journal of Materials Science Materials in Medicine · doi:10.1007/s10856-019-6218-x · Cited by 48
3D in vitro models of skeletal muscle: myopshere, myobundle and bioprinted muscle construct
Dessauge, F., Schleder, C., Perruchot, M., & Rouger, K. (2021) · Veterinary Research · doi:10.1186/s13567-021-00942-w · Cited by 48
Recent advances in materials and applications for bioelectronic and biorobotic systems
Phillips, J. W., Promiński, A., & Tian, B. (2022) · Open Research (University of Surrey) · doi:10.1002/viw.20200157 · Cited by 49
A Focus on “Bio” in Bio–Nanoscience: The Impact of Biological Factors on Nanomaterial Interactions
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helixCAM: A platform for programmable cellular assembly in bacteria and human cells
Chao, G., Wannier, T. M., Gutierrez, C. S., Borders, N. C., Appleton, E., Chadha, A., Lebar, T., & Church, G. M. (2022) · Cell · doi:10.1016/j.cell.2022.08.012 · Cited by 47
Ren, C., Chen, W., Liao, Y. J., Huang, Y., Yu, C., Chen, T., Zeng, Q., Yang, Y., Huang, R., Liu, T., et al. (2024) · Advanced Materials · doi:10.1002/adma.202416432 · Cited by 49
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He, S., Liang, W., Tang, Y., Zhang, J., Wang, R., Quan, L., Ouyang, Y., Huang, R., Dou, R., & Wu, D. (2025) · Nature Communications · doi:10.1038/s41467-025-58062-4 · Cited by 49
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Antill‐O'Brien, N., Bourke, J., & O’Connell, C. D. (2019) · Materials · doi:10.3390/ma12193218 · Cited by 49
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Luo, Z., Zhou, X., Mandal, K., He, N., Wennerberg, W., Qu, M., Jiang, X., Sun, W., & Khademhosseini, A. (2021) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2021.113839 · Cited by 49
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Schiros, T. N., Mosher, C. Z., Zhu, Y., Bina, T., Gomez, V., Lee, C. L., Lu, H. H., & Obermeyer, A. C. (2021) · Chem · doi:10.1016/j.chempr.2021.10.012 · Cited by 50
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Furlani, F., Campodoni, E., Sangiorgi, N., Montesi, M., Sanson, A., Sandri, M., & Panseri, S. (2022) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2022.10.122 · Cited by 47
Recent Advances in Generation of In Vitro Cardiac Organoids
Sahara, M. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24076244 · Cited by 47
Lotz, O., McKenzie, D. R., Bilek, M., & Akhavan, B. (2023) · Progress in Materials Science · doi:10.1016/j.pmatsci.2023.101124 · Cited by 46
Lu, G., Tang, R., Nie, J., & Zhu, X. (2024) · Macromolecular Rapid Communications · doi:10.1002/marc.202300661 · Cited by 46
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Guzzi, E. A., Bovone, G., & Tibbitt, M. W. (2019) · Small · doi:10.1002/smll.201905421 · Cited by 45
Switching between Elasticity and Plasticity by Network Strength Competition
Ming, Z., Pang, Y., & Liu, J. (2019) · Advanced Materials · doi:10.1002/adma.201906870 · Cited by 46
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Turnbull, G. S., Clarke, J., Picard, F., Zhang, W., Riches, P. E., Li, B., & Shu, W. (2020) · Medical Engineering & Physics · doi:10.1016/j.medengphy.2020.06.003 · Cited by 48
Constructing biomimetic liver models through biomaterials and vasculature engineering
Lv, W., Zhou, H., Aazmi, A., Yu, M., Xu, X., Yang, H., Huang, Y. Y. S., & Ma, L. (2022) · Regenerative Biomaterials · doi:10.1093/rb/rbac079 · Cited by 45
Uddin, M. N., Ali, A., Jobaer, M., Mahedi, S. I., Krishnamoorthy, A., & Bhuiyan, M. A. R. (2024) · Materials Advances · doi:10.1039/d4ma00219a · Cited by 47
Cell-guiding microporous hydrogels by photopolymerization-induced phase separation
Müller, M. Z., Bernero, M., Xie, C., Qiu, W., Oggianu, E., Rabut, L., Michaels, T. C. T., Style, R. W., Müller, R., & Qin, X. (2025) · Nature Communications · doi:10.1038/s41467-025-60113-9 · Cited by 47
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Computational study of extrusion bioprinting with jammed gelatin microgel-based composite ink
Song, K., Zhang, D., Yin, J., & Huang, Y. H. (2021) · Additive manufacturing · doi:10.1016/j.addma.2021.101963 · Cited by 48
Maiullari, F., Chirivì, M., Costantini, M., Ferretti, A. M., Recchia, S., Maiullari, S., Milan, M., Presutti, D., Pace, V., Raspa, M., et al. (2021) · Biofabrication · doi:10.1088/1758-5090/abdacf · Cited by 44
Sharma, A., Sharma, P., & Roy, S. (2021) · Soft Matter · doi:10.1039/d0sm02202k · Cited by 44
Ninan, N., Joseph, B., Visalakshan, R. M., Bright, R., Denoual, C., Zilm, P. S., Dalvi, Y. B., Priya, P. V., Mathew, A. P., Grohens, Y., et al. (2021) · Materials Advances · doi:10.1039/d1ma00444a · Cited by 44
3D printing of implantable elastic PLCL copolymer scaffolds
Liu, W., Feng, Z., Ou-Yang, W., Pan, X., Wang, X., Huang, P., Zhang, C., Kong, D., & Wang, W. (2020) · Soft Matter · doi:10.1039/c9sm02396h · Cited by 43
Rheological characterization of cell-laden alginate-gelatin hydrogels for 3D biofabrication
Gregory, T., Benhal, P., Scutte, A., Quashie, D., Harrison, K., Cargill, C., Grandison, S., Savitsky, M. J., Ramakrishnan, S., & Ali, J. (2022) · Journal of the mechanical behavior of biomedical materials/Journal of mechanical behavior of biomedical materials · doi:10.1016/j.jmbbm.2022.105474 · Cited by 42
Towards 3D Bioprinted Spinal Cord Organoids
Han, Y., King, M., Tikhomirov, E., Barasa, P., Souza, C. D. S., Lindh, J., Baltriukienė, D., Ferraiuolo, L., Azzouz, M., Gullo, M. R., et al. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23105788 · Cited by 45
Biofabrication Strategies for Oral Soft Tissue Regeneration
Rahimnejad, M., Makkar, H., Dal‐Fabbro, R., Malda, J., Sriram, G., & Bottino, M. C. (2024) · Advanced Healthcare Materials · doi:10.1002/adhm.202304537 · Cited by 42
Zhang, T., Chen, H., Zhang, Y., Zan, Y., Ni, T., Liu, M., & Pei, R. (2018) · Colloids and Surfaces B Biointerfaces · doi:10.1016/j.colsurfb.2018.11.050 · Cited by 42
New Frontiers for Biofabrication and Bioreactor Design in Microphysiological System Development
Parrish, J., Lim, K. S., Zhang, B., Radisic, M., & Woodfield, T. B. F. (2019) · Trends in biotechnology · doi:10.1016/j.tibtech.2019.04.009 · Cited by 42
3D-extrusion printing of stable constructs composed of photoresponsive polypeptide hydrogels
Murphy, R. D., Kimmins, S. D., Hibbitts, A., & Heise, A. (2019) · Polymer Chemistry · doi:10.1039/c9py00796b · Cited by 42
Patient-Derived In Vitro Models for Drug Discovery in Colorectal Carcinoma
Ramzy, G. M., Koessler, T., Ducrey, E., McKee, T., Ris, F., Buchs, N. C., Rubbia‐Brandt, L., Dietrich, P., & Nowak-Sliwinska, P. M. (2020) · Cancers · doi:10.3390/cancers12061423 · Cited by 45
Single Cell Bioprinting with Ultrashort Laser Pulses
Zhang, J., Byers, P., Erben, A., Frank, C., Schulte‐Spechtel, L., Heymann, M., Docheva, D., Huber, H. P., Sudhop, S., & Clausen‐Schaumann, H. (2021) · Advanced Functional Materials · doi:10.1002/adfm.202100066 · Cited by 42
Wang, M., Li, W., Luo, Z., Tang, G., Mu, X., Kuang, X., Guo, J., Zhao, Z., Flores, R. S., Jiang, Z., et al. (2022) · Biofabrication · doi:10.1088/1758-5090/ac5936 · Cited by 42
Cellulose-based 3D printing bio-inks for biomedical applications: A review
Sharma, C., Raza, M. A., Purohit, S. D., Pathak, P., Gautam, S., Corridon, P. R., & Han, S. S. (2025) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2025.141174 · Cited by 49
Hoffmann, A., Leonards, H., Tobies, N., Pongratz, L., Kreuels, K., Kreimendahl, F., Apel, C., Wehner, M. M., & Nottrodt, N. (2017) · Journal of Tissue Engineering · doi:10.1177/2041731417744485 · Cited by 42
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Self-citationAldana, A. A., Morgan, F. L. C., Houben, S., Pitet, L. M., Moroni, L., & Baker, M. B. (2021) · Journal of Polymer Science · doi:10.1002/pol.20210554 · Cited by 40
Self-citation3D bioprinting of hydrogel‐based biomimetic microenvironments
Luo, Y., Wei, X., & Huang, P. (2018) · Journal of Biomedical Materials Research Part B Applied Biomaterials · doi:10.1002/jbm.b.34262 · Cited by 41
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Viola, M., Piluso, S., Gröll, J., Vermonden, T., Malda, J., & Castilho, M. D. (2021) · Advanced Healthcare Materials · doi:10.1002/adhm.202101021 · Cited by 38
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Alcala‐Orozco, C. R., Cui, X., Hooper, G. J., Lim, K. S., & Woodfield, T. B. F. (2021) · Acta Biomaterialia · doi:10.1016/j.actbio.2021.03.008 · Cited by 40
Kapr, J., Petersilie, L., Distler, T., Lauria, I., Bendt, F., Sauter, C. M., Boccaccini, A. R., Rose, C. R., & Fritsche, E. (2021) · Advanced Healthcare Materials · doi:10.1002/adhm.202100131 · Cited by 40
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Wu, H., Liao, H., Li, F., Lee, J., Hu, P., Shao, W., Li, X., & Ling, D. (2020) · Nano Select · doi:10.1002/nano.202000021 · Cited by 37
Rodrigues, J., Sarmento, B. F. C. C., & Pereira, C. L. (2022) · In vitro models · doi:10.1007/s44164-022-00008-x · Cited by 41
Qiu, Z., Zhu, H., Wang, Y., Kasimu, A., Li, D., & He, J. (2023) · Bio-Design and Manufacturing · doi:10.1007/s42242-022-00225-z · Cited by 36
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Jiang, Z., Jin, B., Liang, Z., Wang, Y., Ren, S., Huang, Y., Li, C., Sun, H., Li, Y., Liu, L., et al. (2024) · Biomaterials · doi:10.1016/j.biomaterials.2024.122681 · Cited by 34
Yang, W., Cai, S., Chen, Y., Liang, W., Lai, Y., Yu, H., Wang, Y., & Liu, L. (2020) · Advanced Materials Technologies · doi:10.1002/admt.201900847 · Cited by 34
Camarero‐Espinosa, S., Tomasina, C., Calore, A. R., & Moroni, L. (2020) · Materials Today Bio · doi:10.1016/j.mtbio.2020.100051 · Cited by 34
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Metadata
- Authors
- Moroni, L., Burdick, J. A., Highley, C., Lee, S. J., Morimoto, Y., Takeuchi, S., & Yoo, J. J.
- Affiliations
- Maastricht University (NL)
- University of Pennsylvania (US)
- Wake Forest University Health Sciences (US)
- Institute of Industrial Science, The University of Tokyo (JP)
- Corresponding author
- Moroni, Lorenzo
- ORCID
- Venue
- Nature Reviews Materials
- Published
- 2018-04-26
- Volume
- 3
- Issue
- 5
- Pages
- 21-37
- ISSN
- 2058-8437 (ISSN-L)
- PMC
- PMC6586020
- OpenAlex
- W2799893835
- Cited by
- 753
- References
- 243
- Citations by year
- 2026: 57 · 2025: 103 · 2024: 89 · 2023: 104 · 2022: 104 · 2021: 129 · 2020: 97 · 2019: 54 · 2018: 13
- FWCI
- 5.97
- Type
- journal article
- Language
- en
- Update notices
- correction (2018-05-03)
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Nature Reviews Materials (publishedVersion, cc-by)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Research Publications (Maastricht University) (submittedVersion, cc-by)
- Research Publications (Maastricht University) (submittedVersion, other-oa)
- Funders
- AO Foundation
- National Institutes of Health
- Japan Society for the Promotion of Science (16H06329)
- National Institute of Biomedical Imaging and Bioengineering (P41EB023833)
- H2020 European Research Council (637308)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Materials Science › Biomaterials › Hydrogels: synthesis, properties, applications
- Keywords
- biofabrication, regenerative medicine, bioprinting, biomaterials, supramolecular materials, photoresponsive materials, tissue engineering, vascular system, foreign body reaction, scalability, organ models
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