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Advanced maturation of human cardiac tissue grown from pluripotent stem cells
Open accessAbstract
Cardiac tissues generated from human induced pluripotent stem cells (iPSCs) can serve as platforms for patient-specific studies of physiology and disease1–6. However, the predictive power of these models is presently limited by the immature state of the cells1,2,5,6. Here we show that this fundamental limitation can be overcome if cardiac tissues are formed from early-stage iPSC-derived cardiomyocytes soon after the initiation of spontaneous contractions and are subjected to physical conditioning with increasing intensity over time. After only four weeks of culture, for all iPSC lines studied, such tissues displayed adult-like gene expression profiles, remarkably organized ultrastructure, physiological sarcomere length (2.2 µm) and density of mitochondria (30%), the presence of transverse tubules, oxidative metabolism, a positive force–frequency relationship and functional calcium handling. Electromechanical properties developed more slowly and did not achieve the stage of maturity seen in adult human myocardium. Tissue maturity was necessary for achieving physiological responses to isoproterenol and recapitulating pathological hypertrophy, supporting the utility of this tissue model for studies of cardiac development and disease. A tissue culture system that provides an increasing intensity of electromechanical stimulation over time enables an in vitro model of cardiac tissue derived from human induced pluripotent stem cells to develop many of the characteristics of adult cardiac tissue.
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“Key genes in the maturation of fatty acid metabolism, sarcomere, and ion channels were also upregulated in CMs from the hvCTS and hvCOC relative to those from the CS and hvCAS (Figure 3C).11,23,36”
From Single-cell RNA sequencing reveals maturation trajectory in human pluripotent stem cell-derived cardiomyocytes in engineered tissues (2023) · cites this paper as [11]
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“This is particularly important in the heart, where the hierarchical organization of cardiac tissues3 is needed to regulate the spatiotemporal dynamics of excitation-contraction coupling, resulting in the cardiac cycle4, 5.”
From Fibre-infused gel scaffolds guide cardiomyocyte alignment in 3D-printed ventricles (2023) · cites this paper as [4]
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“cardiac tissue engineering can provide a unique cellular niche environment to promote in vitro maturation of differentiated CMs.11,12,13,14,15,16,17,18”
From Single-cell RNA sequencing reveals maturation trajectory in human pluripotent stem cell-derived cardiomyocytes in engineered tissues (2023) · cites this paper as [11]
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“Fibrillar hydrogels such as collagen and fibrin are compatible with cardiac tissue engineering as the cells can rapidly contract the matrix to form high cell density tissues49”
From 3D bioprinting of high cell-density heterogeneous tissue models through spheroid fusion within self-healing hydrogels (2021) · cites this paper as [49]
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“The structure and function of iPSC-CMs can be matured using mechanical and electrical stimulation42,49, prolonged culture periods64,65, and also co-culture in the presence of CFs or endothelial cells66.”
From 3D bioprinting of high cell-density heterogeneous tissue models through spheroid fusion within self-healing hydrogels (2021) · cites this paper as [49]
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“The sarcomere length of the 17-day-old CMs in LVDcardiac tissues displayed a statistically significant increase compared with that of day 3, extended from ~1.74 µm to ~2.02 µm, whose value was quite close to that in adult human CMs (2.2 µm) 42 (Fig. 5j).”
From Leaf-venation-directed cellular alignment for macroscale cardiac constructs with tissue-like functionalities (2023) · cites this paper as [42]
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“While these approaches allow for high control of the end construct, they tend to be expensive, work-intensive, and not readily scalable.”
From Self-assembling human heart organoids for the modeling of cardiac development and congenital heart disease (2021) · cites this paper as [12]
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Cites
Cardiac excitation–contraction coupling
Bers, D., & Despa, S. (2002) · Nature · doi:10.1038/415198a · Cited by 4,927
Biowire: a platform for maturation of human pluripotent stem cell–derived cardiomyocytes
Nunes, S. S., Miklas, J. W., Liu, J., Aschar-Sobbi, R., Xiao, Y., Zhang, B., Jiang, J., Massé, S., Gagliardi, M., Hsieh, A., et al. (2013) · Nature Methods · doi:10.1038/nmeth.2524 · Cited by 952
Engineering Adolescence: Maturation of Human Pluripotent Stem Cell–Derived Cardiomyocytes
Yang, X., Pabon, L., & Murry, C. E. (2014) · Circulation Research · doi:10.1161/circresaha.114.300558 · Cited by 939
Radisic, M., Park, H., Shing, H., Consi, T. R., Schöen, F. J., Langer, R., Freed, L. E., & Vunjak‐Novakovic, G. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0407817101 · Cited by 913
Self-citationWang, G., McCain, M. L., Yang, L., He, A., Pasqualini, F. S., Agarwal, A., Yuan, H., Jiang, D., Zhang, D., Zangi, L., et al. (2014) · Nature Medicine · doi:10.1038/nm.3545 · Cited by 837
Ma, J., Guo, L., Fiene, S., Anson, B. D., Thomson, J. A., Kamp, T. J., Kolaja, K. L., Swanson, B. J., & January, C. T. (2011) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.00694.2011 · Cited by 787
Structural and Functional Maturation of Cardiomyocytes Derived from Human Pluripotent Stem Cells
Lundy, S. D., Zhu, W.-Z., Regnier, M., & Laflamme, M. A. (2013) · Stem Cells and Development · doi:10.1089/scd.2012.0490 · Cited by 738
Using induced pluripotent stem cells to investigate cardiac phenotypes in Timothy syndrome
Yazawa, M., Hsueh, B., Jia, X., Pasca, A. M., Bernstein, J. A., Hallmayer, J., & Dolmetsch, R. E. (2011) · Nature · doi:10.1038/nature09855 · Cited by 677
Self-citationGrowth of engineered human myocardium with mechanical loading and vascular coculture
Tulloch, N. L., Muskheli, V., Razumova, M. V., Korte, F. S., Regnier, M., Hauch, K. D., Pabon, L., Reinecke, H., & Murry, C. E. (2011) · Circulation Research · doi:10.1161/circresaha.110.237206 · Cited by 666
Lopaschuk, G. D., & Jaswal, J. S. (2010) · Journal of Cardiovascular Pharmacology · doi:10.1097/fjc.0b013e3181e74a14 · Cited by 636
Cited by
Sorted by citations
Items 201–300 of 999
Keung, W., Chan, P. K. W., Backeris, P. C., Lee, E. K., Wong, N., Wong, A. O. T., Wong, G. K. Y., Chan, C. W. Y., Fermini, B., Costa, K. D., et al. (2019) · Clinical Pharmacology & Therapeutics · doi:10.1002/cpt.1385 · Cited by 54
hiPSC-Derived Cardiac Tissue for Disease Modeling and Drug Discovery
Li, J., Hua, Y., Miyagawa, S., Zhang, J., Li, L., Liu, L., & Sawa, Y. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21238893 · Cited by 56
Shen, S. H., Sewanan, L. R., Shao, S., Halder, S. S., Stankey, P. P., Li, X., & Campbell, S. G. (2022) · Stem Cell Reports · doi:10.1016/j.stemcr.2022.07.006 · Cited by 49
Kim, H. N., Habbit, N. L., Su, C., Choi, N., Ahn, E. H., Lipke, E. A., & Kim, D. (2019) · Advanced Functional Materials · doi:10.1002/adfm.201807553 · Cited by 55
Engineered Macroscale Cardiac Constructs Elicit Human Myocardial Tissue-like Functionality
Valls-Margarit, M., Iglesias‐García, O., Guglielmo, C. D., Sarlabous, L., Tadevosyan, K., Paoli, R., Comelles, J., Blanco-Almazán, D., Jiménez‐Delgado, S., Castillo‐Fernández, Ó., et al. (2019) · Stem Cell Reports · doi:10.1016/j.stemcr.2019.05.024 · Cited by 54
Biomaterials and Culture Systems for Development of Organoid and Organ-on-a-Chip Models
D’Costa, K. A., Kosic, M., Lam, A., Moradipour, A., Zhao, Y., & Radisic, M. (2020) · Annals of Biomedical Engineering · doi:10.1007/s10439-020-02498-w · Cited by 54
Visualization of β-adrenergic receptor dynamics and differential localization in cardiomyocytes
Bathe‐Peters, M., Gmach, P., Boltz, H.-H., Einsiedel, J., Gotthardt, M., Hübner, H., Gmeiner, P., Lohse, M. J., & Annibale, P. (2021) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2101119118 · Cited by 54
Liver–heart on chip models for drug safety
Ferrari, E., & Rasponi, M. (2021) · APL Bioengineering · doi:10.1063/5.0048986 · Cited by 53
Doherty, E. L., Aw, W. Y., Hickey, A. J., & Polacheck, W. J. (2021) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2021.624435 · Cited by 53
Huebsch, N. (2019) · Acta Biomaterialia · doi:10.1016/j.actbio.2019.05.055 · Cited by 54
Ribeiro, A. J. S., Guth, B. D., Engwall, M. J., Eldridge, S. R., Foley, C. M., Guo, L., Gintant, G. A., Koerner, J. E., Parish, S. T., Pierson, J. B., et al. (2019) · Frontiers in Pharmacology · doi:10.3389/fphar.2019.00934 · Cited by 54
Engineered models of the human heart: Directions and challenges
Stein, J. M., Mummery, C. L., & Bellin, M. (2020) · Stem Cell Reports · doi:10.1016/j.stemcr.2020.11.013 · Cited by 55
Human iPSC-engineered cardiac tissue platform faithfully models important cardiac physiology
de Lange, W. J., Farrell, E. T., Kreitzer, C. R., Jacobs, D. R., Lang, D., Glukhov, A. V., & Ralphe, J. C. (2021) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.00941.2020 · Cited by 54
Körner, A., Mosqueira, M., Hecker, M., & Ullrich, N. D. (2021) · Frontiers in Physiology · doi:10.3389/fphys.2021.710619 · Cited by 52
Radke, M. H., Lisakowski, V. B., Britto‐Borges, T., Kubli, D. A., Jüttner, R., Parakkat, P., Carballo, J. L., Huettemeister, J., Liss, M., Hansen, A., et al. (2021) · Science Translational Medicine · doi:10.1126/scitranslmed.abe8952 · Cited by 50
Design of an Integrated Microvascularized Human Skin-on-a-Chip Tissue Equivalent Model
Jones, C. F. E., Ciò, S. D., Connelly, J. T., & Gautrot, J. E. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2022.915702 · Cited by 53
Lu, B., Ye, M., Xia, J., Zhang, Z., Xiong, Z., & Zhang, T. (2023) · Advanced Healthcare Materials · doi:10.1002/adhm.202300607 · Cited by 52
Multimodal Charting of Molecular and Functional Cell States via in situ Electro-Seq
Li, Q., Lin, Z., Liu, R., Tang, X., Huang, J., He, Y., Sui, X., Tian, W., Shen, H., Zhou, H., et al. (2022) · Cell · doi:10.1016/j.cell.2023.03.023 · Cited by 53
Pourrier, M., & Fedida, D. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21020657 · Cited by 53
Cell type–specific microRNA therapies for myocardial infarction
Liu, B., Wang, B., Zhang, X., Lock, R., Nash, T., & Vunjak-Novakovic, G. (2021) · Science Translational Medicine · doi:10.1126/scitranslmed.abd0914 · Cited by 53
Self-citationAdvances in cardiac tissue engineering and heart‐on‐a‐chip
Kieda, J., Shakeri, A., Landau, S., Wang, E. Y., Zhao, Y., Lai, B. F., Okhovatian, S., Wang, Y., Jiang, R., & Radisic, M. (2023) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.37633 · Cited by 54
Decellularized Human Dermal Matrix as a Biological Scaffold for Cardiac Repair and Regeneration
Belviso, I., Romano, V., Sacco, A. M., Ricci, G., Massai, D., Cammarota, M., Catizone, A., Schiraldi, C., Nurzyńska, D., Terzini, M., et al. (2020) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2020.00229 · Cited by 51
Liu, Y., Bai, H., Guo, F., Thai, P. N., Luo, X., Zhang, P., Yang, C. C., Feng, X., Zhu, D., Guo, J. X., et al. (2020) · Aging · doi:10.18632/aging.103088 · Cited by 52
Retinoic acid promotes metabolic maturation of human Embryonic Stem Cell-derived Cardiomyocytes
Miao, S., Zhao, D., Wang, X., Ni, X., Fang, X., Yu, M., Ye, L., Yang, J., Wu, H., Han, X., et al. (2020) · Theranostics · doi:10.7150/thno.44146 · Cited by 51
Song, Y., Uchida, H., Sharipol, A., Piraino, L. R., Mereness, J. A., Ingalls, M. H., Rebhahn, J. A., Newlands, S. D., DeLouise, L. A., Ovitt, C. E., et al. (2021) · Communications Biology · doi:10.1038/s42003-021-01876-x · Cited by 53
A framework for developing sex-specific engineered heart models
Lock, R., Al Asafen, H., Fleischer, S., Tamargo, M., Zhao, Y., Radisic, M., & Vunjak-Novakovic, G. (2021) · Nature Reviews Materials · doi:10.1038/s41578-021-00381-1 · Cited by 50
Self-citationBranco, M. A., Dias, T. P., Cabral, J. M. S., Pinto‐do‐Ó, P., & Diogo, M. M. (2022) · Nature Communications · doi:10.1038/s41467-022-34730-7 · Cited by 51
Wang, X. S., Feiner, R., Luan, H., Zhang, Q., Zhao, S., Zhang, Y., Han, M., Li, Y., Sun, R., Wang, H., et al. (2020) · Extreme Mechanics Letters · doi:10.1016/j.eml.2020.100634 · Cited by 50
Bioengineered optogenetic model of human neuromuscular junction
Vila, O. F., Chavez, M., Ma, S. P., Yeager, K. J., Zholudeva, L. V., Colon, J., Qu, Y., Nash, T. R., Lai, C., Feliciano, C. M., et al. (2021) · Biomaterials · doi:10.1016/j.biomaterials.2021.121033 · Cited by 50
Self-citationLi, W., Luo, X., Strano, A., Arun, S., Gamm, O., Poetsch, M. S., Hasse, M., Steiner, R.-P., Fischer, K., Pöche, J., et al. (2025) · Nature Communications · doi:10.1038/s41467-025-58044-6 · Cited by 49
Lemme, M., Braren, I., Prondzynski, M., Aksehirlioglu, B., Ulmer, B. M., Schulze, M. L., Ismaili, D., Meyer, C. G., Hansen, A., Christ, T., et al. (2019) · Cardiovascular Research · doi:10.1093/cvr/cvz245 · Cited by 49
NanoMEA: A Tool for High-Throughput, Electrophysiological Phenotyping of Patterned Excitable Cells
Smith, A. S. T., Choi, E., Gray, K., Macadangdang, J. R., Ahn, E. H., Clark, E. C., Laflamme, M. A., Wu, J. C., Murry, C. E., Tung, L., et al. (2019) · Nano Letters · doi:10.1021/acs.nanolett.9b04152 · Cited by 49
Takada, T., Sasaki, D., Matsuura, K., Miura, K., Sakamoto, S., Gotô, H., Ohya, T., Iida, T., Homma, J., Shimizu, T., et al. (2021) · Biomaterials · doi:10.1016/j.biomaterials.2021.121351 · Cited by 47
Yang, H., Yang, Y., Kiskin, F. N., Shen, M., & Zhang, J. Z. (2023) · Stem Cell Research & Therapy · doi:10.1186/s13287-023-03470-w · Cited by 54
Macrophages enhance contractile force in iPSC-derived human engineered cardiac tissue
Lock, R. I., Graney, P. L., Tavakol, D. N., Nash, T. R., Kim, Y., Sanchez, E., Morsink, M. A. J., Ning, D., Chen, C., Fleischer, S., et al. (2024) · Cell Reports · doi:10.1016/j.celrep.2024.114302 · Cited by 50
Self-citationJackman, C. P., Li, H., & Bursac, N. (2018) · Acta Biomaterialia · doi:10.1016/j.actbio.2018.08.003 · Cited by 49
Three-dimensional microengineered models of human cardiac diseases
Veldhuizen, J., Migrino, R. Q., & Nikkhah, M. (2019) · Journal of Biological Engineering · doi:10.1186/s13036-019-0155-6 · Cited by 49
Experiments and modeling of the viscoelastic behavior of polymeric gels
Bosnjak, N., Nadimpalli, S. P. V., OKUMURA, D., & Chester, S. A. (2019) · Journal of the Mechanics and Physics of Solids · doi:10.1016/j.jmps.2019.103829 · Cited by 47
Extracellular Vesicles in Cardiac Regeneration: Potential Applications for Tissues-on-a-Chip
Wagner, K. T., Nash, T. R., Liu, B., Vunjak‐Novakovic, G., & Radisic, M. (2020) · Trends in biotechnology · doi:10.1016/j.tibtech.2020.08.005 · Cited by 49
Self-citationPrecision Medicine and cardiac channelopathies: when dreams meet reality
Gnecchi, M., Sala, L., & Schwartz, P. J. (2021) · European Heart Journal · doi:10.1093/eurheartj/ehab007 · Cited by 45
Wei, X., Zhuang, L., Li, H., He, C., Wan, H., Hu, N., & Wang, P. (2020) · Small · doi:10.1002/smll.202005828 · Cited by 49
Functional isolation, culture and cryopreservation of adult human primary cardiomyocytes
Zhou, B., Shi, X., Tang, X., Zhao, Q., Wang, L., Yao, F., Hou, Y., Wang, X., Feng, W., Wang, L., et al. (2022) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-022-01044-5 · Cited by 46
Zhou, Q., Xu, H., Liang, Y., Liang, Y., Zhang, X., Tan, B., Yi, Q., Tian, J., & Zhu, J. (2020) · Genes & Diseases · doi:10.1016/j.gendis.2020.12.006 · Cited by 46
Sakamoto, T., & Kelly, D. P. (2023) · Journal of Molecular and Cellular Cardiology · doi:10.1016/j.yjmcc.2023.12.008 · Cited by 50
Ewoldt, J. K., DePalma, S. J., Jewett, M. E., Karakan, M. Ç., Lin, Y.-M., Hashemian, P. M., Gao, X., Lou, L., McLellan, M. A., Tabares, J., et al. (2024) · Nature Methods · doi:10.1038/s41592-024-02480-7 · Cited by 57
Sewanan, L. R., Shen, S. H., & Campbell, S. G. (2021) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.00325.2020 · Cited by 46
Schneider, O., Moruzzi, A., Fuchs, S., Grobel, A., Schulze, H. S., Mayr, T., & Loskill, P. (2022) · Materials Today Bio · doi:10.1016/j.mtbio.2022.100280 · Cited by 46
Du, Y., Wang, H., Zhang, T., Wen, W., Li, Z., Bi, M., & Liu, J. (2021) · Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy · doi:10.1016/j.saa.2021.120390 · Cited by 45
Current research trends and challenges in tissue engineering for mending broken hearts
Qasim, M., Palaniappan, A., Powell, H. M., & Khan, M. (2019) · Life Sciences · doi:10.1016/j.lfs.2019.05.012 · Cited by 46
Lou, X., Zhao, M., Fan, C., Fast, V. G., Valarmathi, M. T., Zhu, W., & Zhang, J. J. (2019) · Cardiovascular Research · doi:10.1093/cvr/cvz179 · Cited by 45
Ruan, H., Liao, Y., Ren, Z., Mao, L., Yao, F., Peng, Y., Ye, Y., Zhang, Z., Li, S., Xu, H., et al. (2019) · BMC Biology · doi:10.1186/s12915-019-0709-6 · Cited by 46
Drug response analysis for scaffold-free cardiac constructs fabricated using bio-3D printer
Arai, K., Murata, D., Takao, S., Nakamura, A., Itoh, M., Kitsuka, T., & Nakayama, K. (2020) · Scientific Reports · doi:10.1038/s41598-020-65681-y · Cited by 48
Cardiac regeneration by direct reprogramming in this decade and beyond
Yamakawa, H., & Ieda, M. (2021) · Inflammation and Regeneration · doi:10.1186/s41232-021-00168-5 · Cited by 45
Current strategies of mechanical stimulation for maturation of cardiac microtissues
Carlos‐Oliveira, M., Lozano-Juan, F., Occhetta, P., Visone, R., & Rasponi, M. (2021) · Biophysical Reviews · doi:10.1007/s12551-021-00841-6 · Cited by 50
Recent progress of iPSC technology in cardiac diseases
Funakoshi, S., & Yoshida, Y. (2021) · Archives of Toxicology · doi:10.1007/s00204-021-03172-3 · Cited by 47
Tamargo, M. A., Nash, T. R., Fleischer, S., Kim, Y., Vila, O. F., Yeager, K. J., Summers, M., Zhao, Y., Lock, R. I., Chavez, M., et al. (2021) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.1c01006 · Cited by 43
Self-citationCardiac Transverse Tubules in Physiology and Heart Failure
Dibb, K. M., Louch, W. E., & Trafford, A. W. (2021) · Annual Review of Physiology · doi:10.1146/annurev-physiol-061121-040148 · Cited by 44
Garay, B. I., Givens, S. E., Abreu, P., Liu, M., Yücel, D., Baik, J., Stanis, N., Rothermel, T. M., Magli, A., Abrahante, J. E., et al. (2022) · Stem Cell Reports · doi:10.1016/j.stemcr.2022.07.003 · Cited by 45
Mechanism based therapies enable personalised treatment of hypertrophic cardiomyopathy
Margara, F., Psaras, Y., Wang, Z. J., Schmid, M., Doste, R., Garfinkel, A. C., Repetti, G. G., Seidman, J. G., Seidman, C. E., Rodríguez, B., et al. (2022) · Scientific Reports · doi:10.1038/s41598-022-26889-2 · Cited by 44
Modeling cardiac fibroblast heterogeneity from human pluripotent stem cell-derived epicardial cells
Fernandes, I. M., Funakoshi, S., Hamidzada, H., Epelman, S., & Keller, G. M. (2023) · Nature Communications · doi:10.1038/s41467-023-43312-0 · Cited by 46
Sung, T., Su, H., Ling, Q., Kumar, S. S., Chang, Y., Hsu, S.-T., & Higuchi, A. (2020) · Biomaterials · doi:10.1016/j.biomaterials.2020.120060 · Cited by 44
DePalma, S. J., Davidson, C. D., Stis, A. E., Helms, A. S., & Baker, B. M. (2020) · Biomaterials Science · doi:10.1039/d0bm01247e · Cited by 46
Drug-screening triboelectric nanogenerator-based strain sensor for cardiomyocyte contractility
Chen, H., Wang, A., Chen, J., Xu, X., Xu, X., Wang, J., Li, C., Meng, M., Li, C., Xu, C., et al. (2023) · Nano Energy · doi:10.1016/j.nanoen.2023.108251 · Cited by 43
Kim, M., Fleres, B., Lovett, J. J., Anfinson, M., Samudrala, S. S. K., Kelly, L. J., Teigen, L. E., Cavanaugh, M., Marquez, M., Geurts, A. M., et al. (2020) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2020.00440 · Cited by 45
Block, T. J., Creech, J., da Rocha, A. M., Marinkovic, M., Ponce‐Balbuena, D., Jiménez‐Vázquez, E. N., Griffey, S., & Herron, T. J. (2020) · Scientific Reports · doi:10.1038/s41598-020-76052-y · Cited by 45
Mao, M., Qu, X., Zhang, Y., Gu, B., Li, C., Liu, R., Li, X., Zhu, H., He, J., & Li, D. (2023) · Nature Communications · doi:10.1038/s41467-023-37716-1 · Cited by 42
Cardiac Tissue Chips (CTCs) for Modeling Cardiovascular Disease
Rogers, A. J., Miller, J. M., Kannappan, R., & Sethu, P. (2019) · IEEE Transactions on Biomedical Engineering · doi:10.1109/tbme.2019.2905763 · Cited by 42
Three-dimensional encapsulation of adult mouse cardiomyocytes in hydrogels with tunable stiffness
Crocini, C., Walker, C. J., Anseth, K. S., & Leinwand, L. A. (2019) · Progress in Biophysics and Molecular Biology · doi:10.1016/j.pbiomolbio.2019.04.008 · Cited by 43
Li, J., Rozwadowska, N., Clark, A. D., Fil, D., Napierala, J. S., & Напиерала, М. (2019) · Stem Cell Research · doi:10.1016/j.scr.2019.101529 · Cited by 45
Heart organoids and tissue models for modeling development and disease
Miyamoto, M., Nam, L. L., Kannan, S., & Kwon, C. (2021) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2021.03.011 · Cited by 45
Maturation of human pluripotent stem cell derived cardiomyocytes in vitro and in vivo
Gomez-Garcia, M. J., Quesnel, E., Al-attar, R., Laskary, A. R., & Laflamme, M. A. (2021) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2021.05.022 · Cited by 43
3D bioprinting of stem cell-laden cardiac patch: A promising alternative for myocardial repair
Das, S., Nam, H., & Jang, J. (2021) · APL Bioengineering · doi:10.1063/5.0030353 · Cited by 43
Silver, S. E., Barrs, R. W., & Mei, Y. (2021) · Frontiers in Cardiovascular Medicine · doi:10.3389/fcvm.2021.707890 · Cited by 42
Functional microvascularization of human myocardium in vitro
King, O., Cruz-Moreira, D., Sayed, A., Kermani, F., Kit-Anan, W., Sunyovszki, I., Wang, B. X., Downing, B., Fourre, J., Hachim, D., et al. (2022) · Cell Reports Methods · doi:10.1016/j.crmeth.2022.100280 · Cited by 42
Crestani, T., Steichen, C., Neri, E. A., Rodrigues, M. V., Fonseca‐Alaniz, M. H., Ormrod, B., Holt, M., Pandey, P., Harding, S. E., Ehler, E., et al. (2020) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2020.09.021 · Cited by 40
Zhang, Y., Yu, T., Ding, J., & Li, Z. (2022) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2022.114798 · Cited by 43
van Loo, B., ten Den, S. A., Araújo‐Gomes, N., de Jong, V., Snabel, R. R., Schot, M., Rivera‐Arbeláez, J. M., Veenstra, G. J. C., Passier, R. C., Kamperman, T., et al. (2023) · Nature Communications · doi:10.1038/s41467-023-42297-0 · Cited by 44
Advances in human organs-on-chips and applications for drug screening and personalized medicine
Zhou, C., Li, Z., Lu, K., Liu, Y., Xuan, L., Mao, H., & Wang, X. (2024) · Fundamental Research · doi:10.1016/j.fmre.2023.12.019 · Cited by 41
de Korte, T., Katili, P. A., Yusof, N. A. N. M., van Meer, B. J., Saleem, U., Burton, F. L., Smith, G. L., Clements, P., Mummery, C. L., Eschenhagen, T., et al. (2019) · The Annual Review of Pharmacology and Toxicology · doi:10.1146/annurev-pharmtox-010919-023309 · Cited by 46
Engineering anisotropic 3D tubular tissues with flexible thermoresponsive nanofabricated substrates
Williams, N. P., Rhodehamel, M., Yan, C., Smith, A. S. T., Jiao, A., Murry, C. E., Scatena, M., & Kim, D. (2020) · Biomaterials · doi:10.1016/j.biomaterials.2020.119856 · Cited by 41
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Dostanić, M., Windt, L. M., Stein, J. M., van Meer, B. J., Bellin, M., Orlova, V. V., Mastrangeli, M., Mummery, C. L., & Sarro, P. M. (2020) · Journal of Microelectromechanical Systems · doi:10.1109/jmems.2020.3011196 · Cited by 39
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Zhang, W., Huang, G., & Xu, F. (2020) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2020.589590 · Cited by 45
Blazeski, A., Lowenthal, J., Zhu, R., Ewoldt, J., Boheler, K. R., & Tung, L. (2019) · Stem Cell Reports · doi:10.1016/j.stemcr.2019.04.002 · Cited by 40
Fang, Y., Sun, W., Zhang, T., & Xiong, Z. (2022) · Biomaterials · doi:10.1016/j.biomaterials.2021.121298 · Cited by 40
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Rheault-Henry, M., White, I., Grover, D., & Atoui, R. (2021) · World Journal of Stem Cells · doi:10.4252/wjsc.v13.i4.236 · Cited by 43
Tang, X., Wang, Z., Hu, S., & Zhou, B. (2022) · Pharmaceutics · doi:10.3390/pharmaceutics14071313 · Cited by 39
Heart-on-a-chip systems: disease modeling and drug screening applications
Butler, D., & Reyes, D. R. (2024) · Lab on a Chip · doi:10.1039/d3lc00829k · Cited by 42
Guidelines for assessing maternal cardiovascular physiology during pregnancy and postpartum
Collins, H. E., Alexander, B. T., Care, A. S., Davenport, M. H., Davidge, S. T., Eghbali, M., Giussani, D. A., Hoes, M. F., Julian, C. G., LaVoie, H. A., et al. (2024) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.00055.2024 · Cited by 46
FOXD1-dependent MICU1 expression regulates mitochondrial activity and cell differentiation
Shanmughapriya, S., Tomar, D., Dong, Z., Slovik, K. J., Nemani, N., Natarajaseenivasan, K., Carvalho, E. J., Lu, C., Corrigan, K., Garikipati, V. N. S., et al. (2018) · Nature Communications · doi:10.1038/s41467-018-05856-4 · Cited by 41
Human iPSCs and Genome Editing Technologies for Precision Cardiovascular Tissue Engineering
Gähwiler, E. K. N., Motta, S. E., Martin, M., Nugraha, B., Hoerstrup, S. P., & Emmert, M. Y. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.639699 · Cited by 42
Zhang, F., Cheng, H., Qu, K.-Y., Qian, X., Lin, Y., Zhang, Y., Qian, S.-C., Huang, N., Cui, C., & Chen, M. (2023) · Materials Today Bio · doi:10.1016/j.mtbio.2023.100626 · Cited by 39
Bodenstein, D. F., Siebiger, G., Zhao, Y., Clasky, A. J., Mukkala, A. N., Beroncal, E. L., Banh, L., Aslostovar, L., Brijbassi, S., Hogan, S. E., et al. (2024) · Stem Cell Research & Therapy · doi:10.1186/s13287-024-03771-8 · Cited by 41
Xu, F., Jin, H., Liu, L., Yang, Y., Cen, J., Wu, Y., Chen, S., & Sun, D. (2024) · Microsystems & Nanoengineering · doi:10.1038/s41378-024-00692-7 · Cited by 42
Hoes, M. F., Bömer, N., & van der Meer, P. (2018) · Stem Cells Translational Medicine · doi:10.1002/sctm.18-0052 · Cited by 43
Experimental Models of Brugada syndrome
Sendfeld, F., Selga, E., Scornik, F. S., Pérez, G. J., Mills, N. L., & Brugada, R. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20092123 · Cited by 41
Cardiomyogenesis Modeling Using Pluripotent Stem Cells: The Role of Microenvironmental Signaling
Leitolis, A., Robert, A. W., Pereira, I. T., Correa, A., & Stimamiglio, M. A. (2019) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2019.00164 · Cited by 41
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Kumar, N., Dougherty, J. A., Manring, H. R., Elmadbouh, I. S., Mergaye, M., Czirók, A., Isai, D. G., Belevych, A. E., Yu, L., Janssen, P. M. L., et al. (2019) · Scientific Reports · doi:10.1038/s41598-019-49653-5 · Cited by 42
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Lu, F., & Pu, W. T. (2020) · Biophysical Reviews · doi:10.1007/s12551-020-00729-x · Cited by 38
Wei, L. J., Li, W., Entcheva, E. G., & Li, Z. (2020) · Lab on a Chip · doi:10.1039/d0lc00615g · Cited by 40
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Afjeh‐Dana, E., Naserzadeh, P., Moradi, E., Hosseini, N., Seifalian, A. M., & Ashtari, B. (2022) · Stem Cell Reviews and Reports · doi:10.1007/s12015-021-10280-1 · Cited by 39
Metadata
- Authors
- Ronaldson-Bouchard, K., Ma, S. P., Yeager, K., Chen, T., Song, L., Sirabella, D., Morikawa, K., Teles, D., Yazawa, M., & Vunjak-Novakovic, G.
- Affiliations
- Columbia University (US)
- Columbia University Irving Medical Center (US)
- ORCID
- Venue
- Nature
- Published
- 2018-04
- Volume
- 556
- Issue
- 7700
- Pages
- 239-243
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- PMC
- PMC5895513
- OpenAlex
- W2794616878
- Semantic Scholar
- 5d586dd663580dea97fa99d0561d694f4f7165cc
- Cited by
- 1,272
- References
- 33
- Citations by year
- 2026: 122 · 2025: 130 · 2024: 138 · 2023: 150 · 2022: 158 · 2021: 196 · 2020: 179 · 2019: 142 · 2018: 55
- FWCI
- 50.60
- Type
- journal article
- Language
- en
- Update notices
- correction (2019-07-31)
- Open access
- bronze
- doi.org
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, other-oa)
- Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, cc-by-nc-nd)
- Funders
- Universidade do Minho
- National Institutes of Health (2r01hl076485-16, EB 17103, HL076485, EB025765)
- Japan Society for the Promotion of Science
- National Heart, Lung, and Blood Institute (R01HL138486, R01-HL076485, HL076485)
- National Institute of Environmental Health Sciences
- National Institute of Arthritis and Musculoskeletal and Skin Diseases
- National Institute of Biomedical Imaging and Bioengineering (EB025765, UG3EB025765, UH3EB017103, UH3EB025765, P41EB002520)
- National Institute of Dental and Craniofacial Research
- National Center for Advancing Translational Sciences
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac electrophysiology and arrhythmias
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Congenital heart defects research
- Keywords
- iPSC-derived cardiomyocytes, induced pluripotent stem cells, electromechanical stimulation, physical conditioning, cardiac hypertrophy, isoproterenol response, calcium handling, sarcomere length, mitochondrial density, T-tubules, force-frequency relationship, patient-specific cardiac models
- MeSH terms
- Adult, Calcium, Cell Differentiation, Energy Metabolism, Heart, Humans, Isoproterenol, Mitochondria, Myocardium, Sarcomeres, Myocytes, Cardiac, Tissue Culture Techniques, Induced Pluripotent Stem Cells, Transcriptome
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