The DOI matches its Crossref record; 60 of the 60 references it cites were found in a registry or an index.
Single-cell spatial immune landscapes of primary and metastatic brain tumours
Open accessAbstract
Single-cell technologies have enabled the characterization of the tumour microenvironment at unprecedented depth and have revealed vast cellular diversity among tumour cells and their niche. Anti-tumour immunity relies on cell–cell relationships within the tumour microenvironment 1,2, yet many single-cell studies lack spatial context and rely on dissociated tissues 3. Here we applied imaging mass cytometry to characterize the immunological landscape of 139 high-grade glioma and 46 brain metastasis tumours from patients. Single-cell analysis of more than 1.1 million cells across 389 high-dimensional histopathology images enabled the spatial resolution of immune lineages and activation states, revealing differences in immune landscapes between primary tumours and brain metastases from diverse solid cancers. These analyses revealed cellular neighbourhoods associated with survival in patients with glioblastoma, which we leveraged to identify a unique population of myeloperoxidase (MPO)-positive macrophages associated with long-term survival. Our findings provide insight into the biology of primary and metastatic brain tumours, reinforcing the value of integrating spatial resolution to single-cell datasets to dissect the microenvironmental contexture of cancer.
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Cites
Radiotherapy Plus Concomitant and Adjuvant Temozolomide for Glioblastoma
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The 2021 WHO classification of tumors of the central nervous system: a summary
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Microenvironmental regulation of tumor progression and metastasis
Quail, D. F., & Joyce, J. A. (2013) · Nature Medicine · doi:10.1038/nm.3394 · Cited by 7,994
Self-citationUnderstanding the tumor immune microenvironment (TIME) for effective therapy
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CSF-1R inhibition alters macrophage polarization and blocks glioma progression
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Self-citationA Perivascular Niche for Brain Tumor Stem Cells
Calabrese, C., Poppleton, H., Kocak, M., Hogg, T. L., Fuller, C., Hamner, B., Oh, E. Y., Gaber, M. W., Finklestein, D., Allen, M., et al. (2007) · Cancer Cell · doi:10.1016/j.ccr.2006.11.020 · Cited by 2,197
A molecular atlas of cell types and zonation in the brain vasculature
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Profiling cell identity and tissue architecture with single-cell and spatial transcriptomics
Gulati, G. S., D’Silva, J. P., Liu, Y., Wang, L., & Newman, A. M. (2025) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-024-00768-2 · Cited by 293
Macrophage-mediated myelin recycling fuels brain cancer malignancy
Kloosterman, D. J., Erbani, J., Boon, M., Farber, M., Handgraaf, S. M., Ando-Kuri, M., Sánchez-López, E., Fontein, B., Mertz, M., Nieuwland, M., et al. (2024) · Cell · doi:10.1016/j.cell.2024.07.030 · Cited by 231
Self-citationEnfield, K. S. S., Colliver, E., Lee, C., Magness, A., Moore, D. A., Sivakumar, M., Grigoriadis, K., Pich, O., Karasaki, T., Hobson, P. S., et al. (2024) · Cancer Discovery · doi:10.1158/2159-8290.cd-23-1380 · Cited by 151
Brain macrophage development, diversity and dysregulation in health and disease
Silvin, A., Qian, J., & Ginhoux, F. (2023) · Cellular & Molecular Immunology · doi:10.1038/s41423-023-01053-6 · Cited by 137
Harnessing human iPSC-microglia for CNS-wide delivery of disease-modifying proteins
Chadarevian, J. P., Davtyan, H., Chadarevian, A. L., Nguyen, J., Capocchi, J. K., Le, L., Escobar, A., Chadarevian, T., Mansour, K., Deynega, E., et al. (2025) · Cell Stem Cell · doi:10.1016/j.stem.2025.03.009 · Cited by 60
Metadata
- Authors
- Karimi, E., Yu, M. W., Maritan, S. M., Perus, L. J. M., Rezanejad, M., Sorin, M., Dankner, M., Fallah, P., Doré, S., Zuo, D., Fiset, B., Kloosterman, D. J., Ramsay, L., Wei, Y., Lam, S., Alsajjan, R., Watson, I. R., Roldan Urgoiti, G., Park, M., Brandsma, D., Senger, D. L., Chan, J. A., Akkari, L., Petrecca, K., Guiot, M.-C., Siegel, P. M., Quail, D. F., & Walsh, L. A.
- Venue
- Nature
- Published
- 2023-02-01
- Volume
- 614
- Issue
- 7948
- Pages
- 555-563
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W4318817212
- Cited by
- 401
- References
- 60
- FWCI
- 30.34
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- The paper
- Karimi, E., Yu, M. W., Maritan, S. M., Perus, L. J. M., Rezanejad, M., Sorin, M., Dankner, M., Fallah, P., Doré, S., Zuo, D., Fiset, B., Kloosterman, D. J., Ramsay, L., Wei, Y., Lam, S., Alsajjan, R., Watson, I. R., Roldan Urgoiti, G., Park, M., . . . Walsh, L. A. (2023). Single-cell spatial immune landscapes of primary and metastatic brain tumours. Nature. https://doi.org/10.1038/s41586-022-05680-3
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
