Cited by 26CRISPR and Genetic EngineeringGenetically Modified Organisms ResearchAgricultural Productivity and Crop Improvement

CRISPR for accelerating genetic gains in under-utilized crops of the drylands: progress and prospects

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Sharma, Kiran K., Palakolanu, Sudhakar Reddy, Bhattacharya, Joorie, Shankhapal, Aishwarya R., Bhatnagar-Mathur, Pooja·2022·Frontiers in Genetics

Abstract

Technologies and innovations are critical for addressing the future food system needs where genetic resources are an essential component of the change process. Advanced breeding tools like "genome editing" are vital for modernizing crop breeding to provide game-changing solutions to some of the "must needed" traits in agriculture. CRISPR/Cas-based tools have been rapidly repurposed for editing applications based on their improved efficiency, specificity and reduced off-target effects. Additionally, precise gene-editing tools such as base editing, prime editing, and multiplexing provide precision in stacking of multiple traits in an elite variety, and facilitating specific and targeted crop improvement. This has helped in advancing research and delivery of products in a short time span, thereby enhancing the rate of genetic gains. A special focus has been on food security in the drylands through crops including millets, teff, fonio, quinoa, Bambara groundnut, pigeonpea and cassava. While these crops contribute significantly to the agricultural economy and resilience of the dryland, improvement of several traits including increased stress tolerance, nutritional value, and yields are urgently required. Although CRISPR has potential to deliver disruptive innovations, prioritization of traits should consider breeding product profiles and market segments for designing and accelerating delivery of locally adapted and preferred crop varieties for the drylands. In this context, the scope of regulatory environment has been stated, implying the dire impacts of unreasonable scrutiny of genome-edited plants on the evolution and progress of much-needed technological advances.

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Crossref · 10.3389/fgene.2022.999207

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  • Multiplex-CRISPR gene editing constructs have potential to accelerate genetic gains in underutilized crops, focusing on traits that enhance tolerance to abiotic stresses.

    “Research is exploring the potential of multiplex-CRISPR gene editing constructs to accelerate genetic gains in underutilized crops, focusing on traits that enhance tolerance to abiotic stresses (Sharma et al., 2022).”

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Research works printed but not linked: 2

  1. Rojas-Vasquez R. Gatica-Arias A. (2020). Use of genome editing technologies for genetic improvement of crops of tropical origin. Plant Cell, Tissue and Organ Culture (PCTOC) 140 (1), 215– 244. 10.1111/pbi.13635 · DOI · Paper page
  2. Sharma B. (2015). Biochemical characterization of pearl millet (Pennisetum glaucum (L.) R. Br.) genotypes for identifying off-odour factors. Hisar, India: CCS Haryana Agricultural University. Ph.D. Thesis.

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Authors
Sharma, K. K., Palakolanu, S. R., Bhattacharya, J., Shankhapal, A. R., & Bhatnagar-Mathur, P.
Affiliations
  • Energy and Resources Institute (IN)
  • International Crops Research Institute for the Semi-Arid Tropics (IN)
  • University of Nottingham (GB)
Corresponding author
Sharma, Kiran K.
Venue
Frontiers in Genetics
Published
2022-10-06
Volume
13
Pages
999207-999207
ISSN
1664-8021 (ISSN-L)
OpenAlex
W4303192285
Cited by
24
References
209
Citations by year
2026: 6 · 2025: 10 · 2024: 5 · 2023: 2 · 2022: 1
FWCI
0.47
Type
journal article
Language
en
Open access
Locations
  • Frontiers in Genetics (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Open Access Repository of ICRISAT (International Crops Research Institute for the Semi-Arid Tropics) (acceptedVersion)
  • Rothamsted Repository (Rothamsted Repository) (acceptedVersion, other-oa)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Consortium of International Agricultural Research Centers
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
  • Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Agricultural Productivity and Crop Improvement
Keywords
CRISPR/Cas genome editing, rainfed agriculture, genetic gain, base editing, prime editing, multiplex genome editing, millet, Eragrostis tef, fonio, quinoa, Vigna subterranea, Cajanus cajan, cassava, stress tolerance, nutritional improvement, yield improvement, food security

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