CRISPR for accelerating genetic gains in under-utilized crops of the drylands: progress and prospects
Open accessAbstract
Technologies and innovations are critical for addressing the future food system needs where genetic resources are an essential component of the change process. Advanced breeding tools like "genome editing" are vital for modernizing crop breeding to provide game-changing solutions to some of the "must needed" traits in agriculture. CRISPR/Cas-based tools have been rapidly repurposed for editing applications based on their improved efficiency, specificity and reduced off-target effects. Additionally, precise gene-editing tools such as base editing, prime editing, and multiplexing provide precision in stacking of multiple traits in an elite variety, and facilitating specific and targeted crop improvement. This has helped in advancing research and delivery of products in a short time span, thereby enhancing the rate of genetic gains. A special focus has been on food security in the drylands through crops including millets, teff, fonio, quinoa, Bambara groundnut, pigeonpea and cassava. While these crops contribute significantly to the agricultural economy and resilience of the dryland, improvement of several traits including increased stress tolerance, nutritional value, and yields are urgently required. Although CRISPR has potential to deliver disruptive innovations, prioritization of traits should consider breeding product profiles and market segments for designing and accelerating delivery of locally adapted and preferred crop varieties for the drylands. In this context, the scope of regulatory environment has been stated, implying the dire impacts of unreasonable scrutiny of genome-edited plants on the evolution and progress of much-needed technological advances.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Multiplex-CRISPR gene editing constructs have potential to accelerate genetic gains in underutilized crops, focusing on traits that enhance tolerance to abiotic stresses.
“Research is exploring the potential of multiplex-CRISPR gene editing constructs to accelerate genetic gains in underutilized crops, focusing on traits that enhance tolerance to abiotic stresses (Sharma et al., 2022).”
From Emerging applications of gene editing technologies for the development of climate-resilient crops (2025) · cites this paper as Sharma et al., 2022
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Multiplex Genome Engineering Using CRISPR/Cas Systems
Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., et al. (2013) · Science · doi:10.1126/science.1231143 · Cited by 15,339 · see citing works
Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage
Komor, A. C., Kim, Y. B., Packer, M. S., Zuris, J. A., & Liu, D. R. (2016) · Nature · doi:10.1038/nature17946 · Cited by 5,437 · see citing works
Search-and-replace genome editing without double-strand breaks or donor DNA
Anzalone, A. V., Randolph, P. B., Davis, J. R., Sousa, A. A., Koblan, L. W., Levy, J. M., Chen, P. J., Wilson, C., Newby, G. A., Raguram, A., et al. (2019) · Nature · doi:10.1038/s41586-019-1711-4 · Cited by 4,767 · see citing works
ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering
Gaj, T., Gersbach, C. A., & Barbas, C. F. (2013) · Trends in Biotechnology · doi:10.1016/j.tibtech.2013.04.004 · Cited by 3,820 · see citing works
Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors
Boch, J., Scholze, H., Schornack, S., Landgraf, A., Hahn, S., Kay, S., Lahaye, T., Nickstadt, A., & Bonas, U. (2009) · Science · doi:10.1126/science.1178811 · Cited by 2,707 · see citing works
RNA-guided editing of bacterial genomes using CRISPR-Cas systems
Jiang, W., Bikard, D., Cox, D., Zhang, F., & Marraffini, L. A. (2013) · Nature Biotechnology · doi:10.1038/nbt.2508 · Cited by 2,429 · see citing works
Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants
Makarova, K. S., Wolf, Y. I., Iranzo, J., Shmakov, S., Alkhnbashi, O. S., Brouns, S. J. J., Charpentier, E., Cheng, D. R., Haft, D. H., Horvath, P., et al. (2019) · Nature Reviews Microbiology · doi:10.1038/s41579-019-0299-x · Cited by 2,328 · see citing works
‘Green revolution’ genes encode mutant gibberellin response modulators
Peng, J., Richards, D. E., Hartley, N. M., Murphy, G. P., Devos, K. M., Flintham, J. E., Beales, J., Fish, L. J., Worland, A. J., Pelica, F., et al. (1999) · Nature · doi:10.1038/22307 · Cited by 2,237 · see citing works
RNA targeting with CRISPR–Cas13
Abudayyeh, O. O., Gootenberg, J. S., Essletzbichler, P., Han, S., Joung, J., Belanto, J. J., Verdine, V., Cox, D. B. T., Kellner, M. J., Regev, A., et al. (2017) · Nature · doi:10.1038/nature24049 · Cited by 2,093 · see citing works
Hilton, I. B., D’Ippolito, A. M., Vockley, C. M., Thakore, P. I., Crawford, G. E., Reddy, T. E., & Gersbach, C. A. (2015) · Nature Biotechnology · doi:10.1038/nbt.3199 · Cited by 1,879 · see citing works
Cox, D. B. T., Gootenberg, J. S., Abudayyeh, O. O., Franklin, B., Kellner, M. J., Joung, J., & Zhang, F. (2017) · Science · doi:10.1126/science.aaq0180 · Cited by 1,806 · see citing works
CRISPR/Cas Genome Editing and Precision Plant Breeding in Agriculture
Chen, K., Wang, Y., Zhang, R., Zhang, H., & Gao, C. (2019) · Annual Review of Plant Biology · doi:10.1146/annurev-arplant-050718-100049 · Cited by 1,643 · see citing works
Zuris, J. A., Thompson, D. B., Shu, Y., Guilinger, J. P., Bessen, J. L., Hu, J. H., Maeder, M. L., Joung, J. K., Chen, Z.-Y., & Liu, D. R. (2015) · Nature Biotechnology · doi:10.1038/nbt.3081 · Cited by 1,460 · see citing works
Diversity, classification and evolution of CRISPR-Cas systems
Koonin, E. V., Makarova, K. S., & Zhang, F. (2017) · Current Opinion in Microbiology · doi:10.1016/j.mib.2017.05.008 · Cited by 1,423 · see citing works
A mutant gibberellin-synthesis gene in rice
Sasaki, A., Ashikari, M., Ueguchi‐Tanaka, M., Itoh, H., Nishimura, A., Swapan, D., Ishiyama, K., Saito, T., Kobayashi, M., Khush, G. S., et al. (2002) · Nature · doi:10.1038/416701a · Cited by 1,414 · see citing works
Jiang, W., Zhou, H., Bi, H., Fromm, M., Yang, B., & Weeks, D. P. (2013) · Nucleic Acids Research · doi:10.1093/nar/gkt780 · Cited by 1,250 · see citing works
Epigenetic regulation of stress responses in plants
Chinnusamy, V., & Zhu, J. (2009) · Current Opinion in Plant Biology · doi:10.1016/j.pbi.2008.12.006 · Cited by 1,164 · see citing works
Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors
Konermann, S., Lotfy, P., Brideau, N. J., Oki, J., Shokhirev, M. N., & Hsu, P. D. (2018) · Cell · doi:10.1016/j.cell.2018.02.033 · Cited by 1,164 · see citing works
OsSPL14 promotes panicle branching and higher grain productivity in rice
Miura, K., Ikeda, M., Matsubara, A., Song, X.-J., Ito, M., Asano, K., Matsuoka, M., Kitano, H., & Ashikari, M. (2010) · Nature Genetics · doi:10.1038/ng.592 · Cited by 1,104 · see citing works
Genome Engineering With Zinc-Finger Nucleases
Carroll, D. (2011) · Genetics · doi:10.1534/genetics.111.131433 · Cited by 1,076 · see citing works
Breeding crops to feed 10 billion
Hickey, L. T., Hafeez, A. N., Robinson, H. M., Jackson, S. A., Leal‐Bertioli, S. C. M., Tester, M. A., Gao, C., Godwin, I. D., Hayes, B. J., & Wulff, B. B. H. (2019) · Nature Biotechnology · doi:10.1038/s41587-019-0152-9 · Cited by 1,075 · see citing works
Diversity and evolution of class 2 CRISPR–Cas systems
Shmakov, S., Smargon, A. A., Scott, D. A., Cox, D. B. T., Pyzocha, N., Yan, W. X., Abudayyeh, O. O., Gootenberg, J. S., Makarova, K. S., Wolf, Y. I., et al. (2017) · Nature Reviews Microbiology · doi:10.1038/nrmicro.2016.184 · Cited by 1,056 · see citing works
Vega‐Gálvez, A., Miranda, M., Vergara, J., Uribe, E., Puente, L., & Martínez, E. A. (2010) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.4158 · Cited by 985 · see citing works
<i>Xanthomonas</i> AvrBs3 Family-Type III Effectors: Discovery and Function
Boch, J., & Bonas, U. (2010) · Annual Review of Phytopathology · doi:10.1146/annurev-phyto-080508-081936 · Cited by 978 · see citing works
Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes
Liang, Z., Chen, K., Li, T., Zhang, Y., Wang, Y., Zhao, Q., Liu, J., Zhang, H., Liu, C., Ran, Y., et al. (2017) · Nature Communications · doi:10.1038/ncomms14261 · Cited by 964 · see citing works
Semidwarf ( sd-1 ), “green revolution” rice, contains a defective gibberellin 20-oxidase gene
Spielmeyer, W., Ellis, M. H., & Chandler, P. M. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.132266399 · Cited by 954 · see citing works
Natural variation in GS5 plays an important role in regulating grain size and yield in rice
Li, Y., Fan, C., Xing, Y., Jiang, Y., Luo, L., Sun, L., Shao, D., Xu, C., Li, X., Xiao, J., et al. (2011) · Nature Genetics · doi:10.1038/ng.977 · Cited by 931 · see citing works
Reference genome sequence of the model plant Setaria
Bennetzen, J. L., Schmutz, J., Wang, H., Percifield, R. J., Hawkins, J., Pontaroli, A. C., Estep, M. C., Feng, L., Vaughn, J. N., Grimwood, J., et al. (2012) · Nature Biotechnology · doi:10.1038/nbt.2196 · Cited by 912 · see citing works
Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion
Zong, Y., Wang, Y., Li, C., Zhang, R., Chen, K., Ran, Y., Qiu, J., Wang, D., & Gao, C. (2017) · Nature Biotechnology · doi:10.1038/nbt.3811 · Cited by 875 · see citing works
Prime genome editing in rice and wheat
Lin, Q., Zong, Y., Xue, C., Wang, S., Jin, S., Zhu, Z., Wang, Y., Anzalone, A. V., Raguram, A., Doman, J. L., et al. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0455-x · Cited by 873 · see citing works
Broad-spectrum resistance to bacterial blight in rice using genome editing
Oliva, R. F., Ji, C., Atienza, G., Huguet‐Tapia, J. C., Pérez‐Quintero, Á. L., Li, T., Eom, J., Li, C., Nguyen, H., Liu, B., et al. (2019) · Nature Biotechnology · doi:10.1038/s41587-019-0267-z · Cited by 860 · see citing works
Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion
Shimatani, Z., Kashojiya, S., Takayama, M., Terada, R., Arazoe, T., Ishii, H., Teramura, H., Yamamoto, T., Komatsu, H., Miura, K., et al. (2017) · Nature Biotechnology · doi:10.1038/nbt.3833 · Cited by 812 · see citing works
Introduction to Quantitative Genetics.
Smith, C. A. B., & Falconer, D. S. (1962) · Biometrika · doi:10.2307/2333514 · Cited by 797 · see citing works
Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion
Nekrasov, V., Wang, C., Win, J., Lanz, C., Weigel, D., & Kamoun, S. (2017) · Scientific Reports · doi:10.1038/s41598-017-00578-x · Cited by 769 · see citing works
Signatures of the Adler–Bell–Jackiw chiral anomaly in a Weyl fermion semimetal
Zhang, C.-L., Xu, S.-Y., Belopolski, I., Yuan, Z., Lin, Z., Tong, B., Bian, G., Alidoust, N., Lee, C.-C., Huang, S.-M., et al. (2016) · Nature Communications · doi:10.1038/ncomms10735 · Cited by 760 · see citing works
Impact of plant domestication on rhizosphere microbiome assembly and functions
Pérez‐Jaramillo, J. E., Mendes, R., & Raaijmakers, J. M. (2015) · Plant Molecular Biology · doi:10.1007/s11103-015-0337-7 · Cited by 735 · see citing works
The genome of Chenopodium quinoa
Jarvis, D. E., Ho, Y. S., Lightfoot, D. J., Schmöckel, S. M., Li, B., Borm, T. J. A., Ohyanagi, H., Mineta, K., Michell, C., Saber, N., et al. (2017) · Nature · doi:10.1038/nature21370 · Cited by 716 · see citing works
A transcription activator-like effector toolbox for genome engineering
Sanjana, N. E., Cong, L., Zhou, Y., Cunniff, M. M., Feng, G., & Zhang, F. (2012) · Nature Protocols · doi:10.1038/nprot.2011.431 · Cited by 628 · see citing works
Monna, L. (2002) · DNA Research · doi:10.1093/dnares/9.1.11 · Cited by 598 · see citing works
Loss of an MDR Transporter in Compact Stalks of Maize br2 and Sorghum dw3 Mutants
Multani, D. S., Briggs, S. P., Chamberlin, M. A., Blakeslee, J. J., Murphy, A. S., & Johal, G. S. (2003) · Science · doi:10.1126/science.1086072 · Cited by 580 · see citing works
Role of New Plant Breeding Technologies for Food Security and Sustainable Agricultural Development
Qaim, M. (2020) · Applied Economic Perspectives and Policy · doi:10.1002/aepp.13044 · Cited by 565 · see citing works
Plant gene editing through de novo induction of meristems
Maher, M. F., Nasti, R. A., Vollbrecht, M., Starker, C. G., Clark, M. D., & Voytas, D. F. (2020) · Nature Biotechnology · doi:10.1038/s41587-019-0337-2 · Cited by 541 · see citing works
Cas9-Guide RNA Directed Genome Editing in Soybean
Li, Z., Liu, Z.-B., Xing, A., Moon, B. P., Koellhoffer, J. P., Huang, L., Ward, R. T., Clifton, E., Falco, S. C., & Cigan, A. M. (2015) · PLANT PHYSIOLOGY · doi:10.1104/pp.15.00783 · Cited by 522 · see citing works
Rapid improvement of domestication traits in an orphan crop by genome editing
Lemmon, Z. H., Reem, N. T., Dalrymple, J., Soyk, S., Swartwood, K., Rodríguez-Leal, D., Eck, J. M. V., & Lippman, Z. B. (2018) · Nature Plants · doi:10.1038/s41477-018-0259-x · Cited by 520 · see citing works
Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion
Li, C., Zong, Y., Wang, Y., Jin, S., Zhang, D., Song, Q., Zhang, R., & Gao, C. (2018) · Genome biology · doi:10.1186/s13059-018-1443-z · Cited by 499 · see citing works
Zhang, Y., Yu, Y., Wang, C., Li, Z.-Y., Liu, Q., Xu, J., Liao, J., Wang, X., Qu, L., Chen, F., et al. (2013) · Nature Biotechnology · doi:10.1038/nbt.2646 · Cited by 458 · see citing works
Efficient CRISPR/Cas9-mediated Targeted Mutagenesis in Populus in the First Generation
Fan, D., Liu, T., Li, C., Jiao, B., Li, S., Hou, Y., & Luo, K. (2015) · Scientific Reports · doi:10.1038/srep12217 · Cited by 457 · see citing works
Yan, W. X., Chong, S., Zhang, H., Makarova, K. S., Koonin, E. V., Cheng, D. R., & Scott, D. A. (2018) · Molecular Cell · doi:10.1016/j.molcel.2018.02.028 · Cited by 456 · see citing works
Jia, G., Huang, X., Zhi, H., Zhao, Y., Zhao, Q., Li, W., Yang, C., Yang, L., Liu, K., Lu, H., et al. (2013) · Nature Genetics · doi:10.1038/ng.2673 · Cited by 453 · see citing works
Lämke, J., Brzezinka, K., Altmann, S., & Bäurle, I. (2015) · The EMBO Journal · doi:10.15252/embj.201592593 · Cited by 437 · see citing works
Conferring resistance to geminiviruses with the CRISPR–Cas prokaryotic immune system
Baltes, N. J., Hummel, A. W., Konečná, E., Čegan, R., Bruns, A. N., Bisaro, D. M., & Voytas, D. F. (2015) · Nature Plants · doi:10.1038/nplants.2015.145 · Cited by 433 · see citing works
Precise Editing of a Target Base in the Rice Genome Using a Modified CRISPR/Cas9 System
Lu, Y., & Zhu, J. (2016) · Molecular Plant · doi:10.1016/j.molp.2016.11.013 · Cited by 423 · see citing works
Bredeson, J. V., Lyons, J. B., Prochnik, S. E., Wu, G. A., Ha, C. M., Edsinger, E., Grimwood, J., Schmutz, J., Rabbi, I. Y., Egesi, C. N., et al. (2016) · Nature Biotechnology · doi:10.1038/nbt.3535 · Cited by 419 · see citing works
Targeted mutagenesis using zinc-finger nucleases in Arabidopsis
Lloyd, A., Plaisier, C. L., Carroll, D., & Drews, G. N. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0409339102 · Cited by 418 · see citing works
Multiplexed heritable gene editing using RNA viruses and mobile single guide RNAs
Ellison, E. E., Nagalakshmi, U., Gamo, M. E., Huang, P., Dinesh‐Kumar, S. P., & Voytas, D. F. (2020) · Nature Plants · doi:10.1038/s41477-020-0670-y · Cited by 377 · see citing works
Gale, M. D., & Youssefian, S. (1985) · Elsevier eBooks · doi:10.1016/b978-0-407-00780-2.50005-9 · Cited by 375 · see citing works
Veillet, F., Perrot, L., Chauvin, L., Kermarrec, M.-P., Guyon‐Debast, A., Chauvin, J.-É., Nogué, F., & Mazier, M. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20020402 · Cited by 370 · see citing works
Gomez, M. A., Lin, Z. D., Moll, T., Chauhan, R. D., Hayden, L., Renninger, K. A., Beyene, G., Taylor, N. J., Carrington, J. C., Staskawicz, B. J., et al. (2018) · Plant Biotechnology Journal · doi:10.1111/pbi.12987 · Cited by 362 · see citing works
Clonal seeds from hybrid rice by simultaneous genome engineering of meiosis and fertilization genes
Wang, C., Liu, Q., Shen, Y. X., Hua, Y., Wang, J., Lin, J., Wu, M., Sun, T., Cheng, Z., Mercier, R., et al. (2019) · Nature Biotechnology · doi:10.1038/s41587-018-0003-0 · Cited by 357 · see citing works
A 4-bp Insertion at ZmPLA1 Encoding a Putative Phospholipase A Generates Haploid Induction in Maize
Liu, C., Li, X., Meng, D., Zhong, Y., Chen, C., Dong, X., Xu, X., Chen, B., Li, W., Li, L., et al. (2017) · Molecular Plant · doi:10.1016/j.molp.2017.01.011 · Cited by 353 · see citing works
Engineering the delivery system for CRISPR-based genome editing
Glass, Z., Lee, M., Li, Y., & Xu, Q. (2018) · Trends in biotechnology · doi:10.1016/j.tibtech.2017.11.006 · Cited by 345 · see citing works
Enhancing genetic gain in the era of molecular breeding
Xu, Y., Li, P., Zou, C., Lu, Y., Xie, C., Zhang, X., Prasanna, B. M., & Olsen, M. S. (2017) · Journal of Experimental Botany · doi:10.1093/jxb/erx135 · Cited by 342 · see citing works
The potential for underutilized crops to improve security of food production
Mayes, S., Massawe, F. J., Alderson, P. G., Roberts, J. A., Azam-Ali, S. N., & Hermann, M. (2011) · Journal of Experimental Botany · doi:10.1093/jxb/err396 · Cited by 333 · see citing works
Engineering herbicide-resistant watermelon variety through CRISPR/Cas9-mediated base-editing
Tian, S., Jiang, L., Cui, X., Zhang, J., Guo, S., Li, M., Zhang, H., Ren, Y., Gong, G., Zong, M., et al. (2018) · Plant Cell Reports · doi:10.1007/s00299-018-2299-0 · Cited by 307 · see citing works
The Cassava Genome: Current Progress, Future Directions
Prochnik, S. E., Marri, P. R., Desany, B. A., Rabinowicz, P. D., Kodira, C. D., Mohiuddin, M., Rodríguez-Zapata, F., Fauquet, C., Tohme, J. M., Harkins, T. T., et al. (2012) · Tropical Plant Biology · doi:10.1007/s12042-011-9088-z · Cited by 303 · see citing works
Efficient targeted mutagenesis of rice and tobacco genomes using Cpf1 from Francisella novicida
Endo, A., Mikami, M., Kaya, H., & Toki, S. (2016) · Scientific Reports · doi:10.1038/srep38169 · Cited by 297 · see citing works
Genome Edited Crops Touch the Market: A View on the Global Development and Regulatory Environment
Menz, J., Modrzejewski, D., Hartung, F., Wilhelm, R., & Sprink, T. (2020) · Frontiers in Plant Science · doi:10.3389/fpls.2020.586027 · Cited by 275 · see citing works
Varshney, R. K., Saxena, R. K., Upadhyaya, H. D., Khan, A. W., Yu, Y., Kim, C., Rathore, A., Kim, D., Kim, J., An, S., et al. (2017) · Nature Genetics · doi:10.1038/ng.3872 · Cited by 262 · see citing works
Genebank genomics bridges the gap between the conservation of crop diversity and plant breeding
Mascher, M., Schreiber, M., Scholz, U., Graner, A., Reif, J. C., & Stein, N. (2019) · Nature Genetics · doi:10.1038/s41588-019-0443-6 · Cited by 260 · see citing works
Genome editing in cotton with the CRISPR/cas9 system
Gao, W., Long, L., Tian, X., Xu, F., Liu, J., Singh, P. K., Botella, J. R., & Song, C. (2017) · Frontiers in Plant Science · doi:10.3389/fpls.2017.01364 · Cited by 254 · see citing works
Martin‐Ortigosa, S., Peterson, D. J., Valenstein, J. S., Lin, V. S. ‐., Trewyn, B. G., Lyznik, L. A., & Wang, K. (2013) · PLANT PHYSIOLOGY · doi:10.1104/pp.113.233650 · Cited by 251 · see citing works
Activities and specificities of CRISPR /Cas9 and Cas12a nucleases for targeted mutagenesis in maize
Lee, K., Zhang, Y., Kleinstiver, B. P., Guo, J. A., Aryee, M. J., Miller, J., Malzahn, A. A., Zarecor, S., Lawrence‐Dill, C. J., Joung, J. K., et al. (2018) · Plant Biotechnology Journal · doi:10.1111/pbi.12982 · Cited by 235 · see citing works
Methods and Applications of CRISPR-Mediated Base Editing in Eukaryotic Genomes
Hess, G. T., Tycko, J., Yao, D., & Bassik, M. C. (2017) · Molecular Cell · doi:10.1016/j.molcel.2017.09.029 · Cited by 234 · see citing works
Base editing in crops: current advances, limitations and future implications
Mishra, R., Joshi, R. K., & Zhao, K. (2019) · Plant Biotechnology Journal · doi:10.1111/pbi.13225 · Cited by 231 · see citing works
Principles, Applications, and Biosafety of Plant Genome Editing Using CRISPR-Cas9
El‐Mounadi, K., Morales-Floriano, M. L., & García-Ruíz, H. (2020) · Frontiers in Plant Science · doi:10.3389/fpls.2020.00056 · Cited by 227 · see citing works
Vetriventhan, M., Azevedo, V., Upadhyaya, H. D., Nirmalakumari, A., Kane‐Potaka, J., Anitha, S., Ceasar, S. A., Muthamilarasan, M., Bhat, B. V., Hariprasanna, K., et al. (2020) · The Nucleus · doi:10.1007/s13237-020-00322-3 · Cited by 222 · see citing works
Genetic mapping of a dominant gene conferring resistance to cassava mosaic disease
Akano, A. O., Dixon, A. G. O., Mba, C., Barrera, E., & Fregene, M. (2002) · Theoretical and Applied Genetics · doi:10.1007/s00122-002-0891-7 · Cited by 221 · see citing works
Editing of an Alpha-Kafirin Gene Family Increases, Digestibility and Protein Quality in Sorghum
Li, A., Jia, S., Yobi, A., Ge, Z., Sato, S. J., Zhang, C., Angelovici, R., Clemente, T. E., & Holding, D. R. (2018) · PLANT PHYSIOLOGY · doi:10.1104/pp.18.00200 · Cited by 215 · see citing works
Development of Plant Prime-Editing Systems for Precise Genome Editing
Xu, R., Li, J., Liu, X., Shan, T., Qin, R., & Wei, P. (2020) · Plant Communications · doi:10.1016/j.xplc.2020.100043 · Cited by 209 · see citing works
CRISPR/Cas9-mediated targeted mutagenesis of GmSPL9 genes alters plant architecture in soybean
Bao, A., Chen, H., Chen, L., Chen, S., Hao, Q., Guo, W., Qiu, D., Shan, Z., Yang, Z., Yuan, S., et al. (2019) · BMC Plant Biology · doi:10.1186/s12870-019-1746-6 · Cited by 204 · see citing works
CRISPR / dC as9 platforms in plants: strategies and applications beyond genome editing
Moradpour, M., & Abdulah, S. N. A. (2019) · Plant Biotechnology Journal · doi:10.1111/pbi.13232 · Cited by 203 · see citing works
Highly efficient heritable genome editing in wheat using an RNA virus and bypassing tissue culture
Li, T., Hu, J., Sun, Y., Li, B., Zhang, D., Li, W., Liu, J., Li, D., Gao, C., Zhang, Y., et al. (2021) · Molecular Plant · doi:10.1016/j.molp.2021.07.010 · Cited by 196 · see citing works
A modern Green Revolution gene for reduced height in wheat
Würschum, T., Langer, S. M., Longin, C. F. H., Tucker, M. R., & Leiser, W. L. (2017) · The Plant Journal · doi:10.1111/tpj.13726 · Cited by 192 · see citing works
Genome Editing in Plants: Exploration of Technological Advancements and Challenges
Vats, S., Kumawat, S., Kumar, V., Patil, G., Joshi, T., Sonah, H., Sharma, T. R., & Deshmukh, R. K. (2019) · Cells · doi:10.3390/cells8111386 · Cited by 191 · see citing works
A CRISPR way for accelerating improvement of food crops
Zhang, Y., Pribil, M., Palmgren, M. B., & Gao, C. (2020) · Nature Food · doi:10.1038/s43016-020-0051-8 · Cited by 189 · see citing works
Advantage of Nanotechnology-Based Genome Editing System and Its Application in Crop Improvement
Ahmar, S., Mahmood, T., Fiaz, S., Mora, F., Shafique, M. S., Chattha, M. S., & Jung, K.-H. (2021) · Frontiers in Plant Science · doi:10.3389/fpls.2021.663849 · Cited by 186 · see citing works
Epigenetics for Crop Improvement in Times of Global Change
Kakoulidou, I., Avramidou, E. V., Baránek, M., Brunel‐Muguet, S., Farrona, S., Johannes, F. M., Kaiserli, E., Lieberman‐Lazarovich, M., Martinelli, F., Mladenov, V., et al. (2021) · Biology · doi:10.3390/biology10080766 · Cited by 177 · see citing works
Estimates of rice lodging using indices derived from UAV visible and thermal infrared images
Liu, T., Li, R., Zhong, X., Jiang, M., Jin, X., Zhou, P., Liu, S., Sun, C., & Guo, W. (2018) · Agricultural and Forest Meteorology · doi:10.1016/j.agrformet.2018.01.021 · Cited by 176 · see citing works
Allele exchange at the EPSPS locus confers glyphosate tolerance in cassava
Hummel, A. W., Chauhan, R. D., Čermák, T., Mutka, A. M., Vijayaraghavan, A., Boyher, A., Starker, C. G., Bart, R. S., Voytas, D. F., & Taylor, N. J. (2017) · Plant Biotechnology Journal · doi:10.1111/pbi.12868 · Cited by 174 · see citing works
Precision genome engineering in rice using prime editing system
Hua, K., Jiang, Y., Tao, X., & Zhu, J. (2020) · Plant Biotechnology Journal · doi:10.1111/pbi.13395 · Cited by 171 · see citing works
Impact of amylose content on starch physicochemical properties in transgenic sweet potato
Zhou, W., Yang, J., Hong, Y., Liu, G., Zheng, J., Gu, Z., & Zhang, P. (2015) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2014.11.003 · Cited by 170 · see citing works
CRISPR ribonucleoprotein-mediated genetic engineering in plants
Zhang, Y., Iaffaldano, B. J., & Qi, Y. (2021) · Plant Communications · doi:10.1016/j.xplc.2021.100168 · Cited by 169 · see citing works
Accelerated ex situ breeding of GBSS - and PTST1 -edited cassava for modified starch
Bull, S. E., Seung, D., Chanez, C., Mehta, D., Kuon, J., Truernit, E., Hochmuth, A., Zurkirchen, I., Zeeman, S. C., Gruissem, W., et al. (2018) · Science Advances · doi:10.1126/sciadv.aat6086 · Cited by 166 · see citing works
Precise genome modification in tomato using an improved prime editing system
Lu, Y., Tian, Y., Shen, R., Yao, Q., Zhong, D., Zhang, X., & Zhu, J. (2020) · Plant Biotechnology Journal · doi:10.1111/pbi.13497 · Cited by 164 · see citing works
Genome editing in plants via designed zinc finger nucleases
Petolino, J. F. (2015) · In Vitro Cellular & Developmental Biology - Plant · doi:10.1007/s11627-015-9663-3 · Cited by 163 · see citing works
Chen, Y., Wang, Z., Ni, H., Xu, Y., Chen, Q., & Jiang, L. (2017) · Science China Life Sciences · doi:10.1007/s11427-017-9021-5 · Cited by 161 · see citing works
Current and future editing reagent delivery systems for plant genome editing
Ran, Y., Liang, Z., & Gao, C. (2017) · Science China Life Sciences · doi:10.1007/s11427-017-9022-1 · Cited by 161 · see citing works
Rabbi, I. Y., Hamblin, M. T., Kumar, P. L., Gedil, M. A., Ikpan, A., Jannink, J., & Kulakow, P. (2014) · Virus Research · doi:10.1016/j.virusres.2013.12.028 · Cited by 154 · see citing works
Biolistic-delivery-based transient CRISPR/Cas9 expression enables in planta genome editing in wheat
Hamada, H., Liu, Y., Nagira, Y., Miki, R., Taoka, N., & Imai, R. (2018) · Scientific Reports · doi:10.1038/s41598-018-32714-6 · Cited by 149 · see citing works
Zdruli, P. (2014) · Land Degradation & Development · doi:10.1002/ldr.2150 · Cited by 146 · see citing works
Whole Genome Association Mapping of Plant Height in Winter Wheat (Triticum aestivum L.)
Zanke, C. D., Ling, J., Plieske, J., Kollers, S., Ebmeyer, E., Korzun, V. N., Argillier, O., Stiewe, G., Hinze, M., Neumann, K., et al. (2014) · PLoS ONE · doi:10.1371/journal.pone.0113287 · Cited by 142 · see citing works
Wolfe, M. D., Rabbi, I. Y., Egesi, C. N., Hamblin, M., Kawuki, R., Kulakow, P., Lozano, R. D., Carpio, D. P. D., Ramu, P., & Jannink, J. (2016) · The Plant Genome · doi:10.3835/plantgenome2015.11.0118 · Cited by 138 · see citing works
Do CRISPR Germline Ethics Statements Cut It?
Brokowski, C. (2018) · The CRISPR Journal · doi:10.1089/crispr.2017.0024 · Cited by 137 · see citing works
Stable Expression of mtlD Gene Imparts Multiple Stress Tolerance in Finger Millet
Hema, R., Vemanna, R. S., Sreeramulu, S., Reddy, C. P., Senthil‐Kumar, M., & Udayakumar, M. (2014) · PLoS ONE · doi:10.1371/journal.pone.0099110 · Cited by 131 · see citing works
Improved plant cytosine base editors with high editing activity, purity, and specificity
Ren, Q., Sretenovic, S., Liu, G., Zhong, Z., Wang, J., Huang, L., Tang, X., Guo, Y., Liu, L., Wu, Y., et al. (2021) · Plant Biotechnology Journal · doi:10.1111/pbi.13635 · Cited by 131 · see citing works
RNA-Targeting CRISPR-Cas systems and their applications
Burmistrz, M., Krakowski, K., & Krawczyk-Balska, A. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21031122 · Cited by 125 · see citing works
Fonio millet genome unlocks African orphan crop diversity for agriculture in a changing climate
Abrouk, M., Ahmed, H. I., Cubry, P., Šimoníková, D., Cauet, S., Pailles, Y., Bettgenhaeuser, J., Gapa, L., Scarcelli, N., Couderc, M., et al. (2020) · Nature Communications · doi:10.1038/s41467-020-18329-4 · Cited by 124 · see citing works
Transgenerational response to stress in plants and its application for breeding
Bilichak, A., & Kovalchuk, I. (2016) · Journal of Experimental Botany · doi:10.1093/jxb/erw066 · Cited by 122 · see citing works
Function genomics of abiotic stress tolerance in plants: a CRISPR approach
Jain, M. (2015) · Frontiers in Plant Science · doi:10.3389/fpls.2015.00375 · Cited by 118 · see citing works
Niu, M., Lin, Y., & Zou, Q. (2021) · Plant Molecular Biology · doi:10.1007/s11103-020-01102-y · Cited by 118 · see citing works
Establishing in planta haploid inducer line by edited SiMTL in foxtail millet (Setaria italica)
Cheng, Z., Sun, Y., Yang, S., Zhi, H., Yin, T., Ma, X., Zhang, H., Diao, X., Guo, Y., Li, X., et al. (2021) · Plant Biotechnology Journal · doi:10.1111/pbi.13584 · Cited by 117 · see citing works
Ceasar, S. A., & Savarimuthu Ignacimuthu, S. J. (2011) · Plant Cell Reports · doi:10.1007/s00299-011-1084-0 · Cited by 112 · see citing works
Class 2 CRISPR/Cas: an expanding biotechnology toolbox for and beyond genome editing
Tang, Y., & Fu, Y. (2018) · Cell & Bioscience · doi:10.1186/s13578-018-0255-x · Cited by 112 · see citing works
Breeding and biotechnological interventions for trait improvement: status and prospects
Singh, R. K., Prasad, A., Muthamilarasan, M., Parida, S. K., & Prasad, M. (2020) · Planta · doi:10.1007/s00425-020-03465-4 · Cited by 112 · see citing works
Bread wheat: a role model for plant domestication and breeding
Venske, E., dos Santos, R. S., Busanello, C., Gustafson, P. J., & de Oliveira, A. C. (2019) · Hereditas · doi:10.1186/s41065-019-0093-9 · Cited by 110 · see citing works
Cassava Genetic Transformation and its Application in BreedingF
Liu, J., Zheng, Q., Ma, Q., Gadidasu, K. K., & Zhang, P. (2011) · Journal of Integrative Plant Biology · doi:10.1111/j.1744-7909.2011.01048.x · Cited by 109 · see citing works
Sorghum Dw2 Encodes a Protein Kinase Regulator of Stem Internode Length
Hilley, J., Weers, B. D., Truong, S. K., McCormick, R. F., Mattison, A. J., McKinley, B. A., Morishige, D. T., & Mullet, J. E. (2017) · Scientific Reports · doi:10.1038/s41598-017-04609-5 · Cited by 107 · see citing works
Exploration of Epigenetics for Improvement of Drought and Other Stress Resistance in Crops: A Review
Sun, C., Ali, K., Yan, K., Fiaz, S., Dormatey, R., Bi, Z., & Bai, J. (2021) · Plants · doi:10.3390/plants10061226 · Cited by 104 · see citing works
Majola, N. G., Gerrano, A. S., & Shimelis, H. (2021) · Agronomy · doi:10.3390/agronomy11071345 · Cited by 104 · see citing works
Genomics-assisted breeding for pigeonpea improvement
Bohra, A., Saxena, K. B., Varshney, R. K., & Saxena, R. K. (2020) · Theoretical and Applied Genetics · doi:10.1007/s00122-020-03563-7 · Cited by 102 · see citing works
Improving Nutritional and Functional Quality by Genome Editing of Crops: Status and Perspectives
Ku, H.-K., & Ha, S. (2020) · Frontiers in Plant Science · doi:10.3389/fpls.2020.577313 · Cited by 102 · see citing works
Genetic and Genomic Resources of Small Millets
Saha, D., Gowda, M. V. C., Arya, L., Verma, M., & Bansal, K. C. (2016) · Critical Reviews in Plant Sciences · doi:10.1080/07352689.2016.1147907 · Cited by 101 · see citing works
Achieving Plant CRISPR Targeting that Limits Off‐Target Effects
Wolt, J. D., Wang, K., Sashital, D. G., & Lawrence‐Dill, C. J. (2016) · The Plant Genome · doi:10.3835/plantgenome2016.05.0047 · Cited by 95 · see citing works
Genome editing opens a new era of genetic improvement in polyploid crops
Zaman, Q. U., Li, C., Cheng, H., & Hu, Q. (2018) · The Crop Journal · doi:10.1016/j.cj.2018.07.004 · Cited by 93 · see citing works
Li, Q., Wu, G., Zhao, Y., Wang, B., Zhao, B., Kong, D., Wei, H., Chen, C., & Wang, H. (2020) · Plant Biotechnology Journal · doi:10.1111/pbi.13429 · Cited by 92 · see citing works
Canadian regulatory perspectives on genome engineered crops
Smyth, S. J. (2016) · GM crops & food · doi:10.1080/21645698.2016.1257468 · Cited by 91 · see citing works
Genome Editing for Sustainable Agriculture in Africa
Tripathi, L., Dhugga, K. S., Ntui, V. O., Runo, S. M., Syombua, E. D., Muiruri, S., Wen, Z., & Tripathi, J. N. (2022) · Frontiers in Genome Editing · doi:10.3389/fgeed.2022.876697 · Cited by 91 · see citing works
An optimized Agrobacterium -mediated transformation procedure for Phaseolus acutifolius A. Gray
Clercq, J. D., Zambre, M., MONTAGU, M. V., Dillen, W., & Angenon, G. (2002) · Plant Cell Reports · doi:10.1007/s00299-002-0518-0 · Cited by 90 · see citing works
Regulatory aspects of gene editing in Argentina
Lema, M. A. (2019) · Transgenic Research · doi:10.1007/s11248-019-00145-2 · Cited by 87 · see citing works
Application of genetic modification and genome editing for developing climate‐smart banana
Tripathi, L., Ntui, V. O., & Tripathi, J. N. (2019) · Food and Energy Security · doi:10.1002/fes3.168 · Cited by 84 · see citing works
Huang, L., Zhang, R., Huang, G., Li, Y., Melaku, G., Zhang, S., Chen, H., Zhao, Y., Zhang, J., Zhang, Y., et al. (2018) · The Crop Journal · doi:10.1016/j.cj.2018.05.005 · Cited by 83 · see citing works
Cassava Bacterial Blight: Using Genomics for the Elucidation and Management of an Old Problem
López, C. E., & Bernal, A. (2012) · Tropical Plant Biology · doi:10.1007/s12042-011-9092-3 · Cited by 81 · see citing works
Regulatory Status of Genome-Edited Organisms Under the Japanese Cartagena Act
Tsuda, M., Watanabe, K., & Ohsawa, R. (2019) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2019.00387 · Cited by 81 · see citing works
Kim, H., Choi, J., & Won, K. (2020) · BMC Plant Biology · doi:10.1186/s12870-020-02665-0 · Cited by 80 · see citing works
Targeted plant improvement through genome editing: from laboratory to field
Miladinović, D., Antunes, M. D. C., Yıldırım, K., Bakhsh, A., Cvejić, S., Kondić‐Špika, A., Marjanović‐Jeromela, A., Opsahl-Sorteberg, H.-G., Zambounis, A. G., & Hilioti, Z. (2021) · Plant Cell Reports · doi:10.1007/s00299-020-02655-4 · Cited by 80 · see citing works
Expert opinions on the regulation of plant genome editing
Lassoued, R., Phillips, P. W. B., Macall, D. M., Hesseln, H., & Smyth, S. J. (2021) · Plant Biotechnology Journal · doi:10.1111/pbi.13597 · Cited by 80 · see citing works
Increasing Food Production in Africa by Boosting the Productivity of Understudied Crops
Tadele, Z., & Assefa, K. (2012) · Agronomy · doi:10.3390/agronomy2040240 · Cited by 79 · see citing works
Targeted modification of plant genomes for precision crop breeding
Hilscher, J., Buerstmayr, H., & Stöger, E. (2016) · Biotechnology Journal · doi:10.1002/biot.201600173 · Cited by 79 · see citing works
Ceasar, S. A., Baker, A., & Savarimuthu Ignacimuthu, S. J. (2017) · Scientific Reports · doi:10.1038/s41598-017-14447-0 · Cited by 79 · see citing works
Oberkofler, V., & Bäurle, I. (2022) · PLANT PHYSIOLOGY · doi:10.1093/plphys/kiac113 · Cited by 79 · see citing works
Neglected and Underutilized Legume Crops: Improvement and Future Prospects
Popoola, J. O., Ojuederie, O. B., Omonhinmin, C. A., & Adegbite, A. E. (2019) · IntechOpen eBooks · doi:10.5772/intechopen.87069 · Cited by 75 · see citing works
Genomics-assisted breeding for boosting crop improvement in pigeonpea (Cajanus cajan)
Pazhamala, L. T., Saxena, R. K., Singh, V. K., Sameerkumar, C. V., Kumar, V. S., Sinha, P., Patel, K., Obala, J., Kaoneka, S. R., Tongoona, P. B., et al. (2015) · Frontiers in Plant Science · doi:10.3389/fpls.2015.00050 · Cited by 74 · see citing works
Genome Editing for Global Food Security
Ma, X., Mau, M., & Sharbel, T. F. (2017) · Trends in biotechnology · doi:10.1016/j.tibtech.2017.08.004 · Cited by 74 · see citing works
Agrobacterium-mediated transformation of Phaseolus acutifolius A. Gray
Dillen, W., Clercq, J. D., Goossens, A., MONTAGU, M. V., & Angenon, G. (1997) · Theoretical and Applied Genetics · doi:10.1007/s001220050394 · Cited by 73 · see citing works
Recent Advances in Grain Crops Research
Shah, F., Khan, S. A., Iqbal, A., Turan, M., & Olgun, M. (2019) · IntechOpen eBooks · doi:10.5772/intechopen.78117 · Cited by 73 · see citing works
Muhammad, I., Rafii, M. Y., Ramlee, S. I., Nazli, M. H., Harun, A. R., Oladosu, Y. A., Musa, I., Arolu, F., Chukwu, S. C., Haliru, B. S., et al. (2020) · Agronomy · doi:10.3390/agronomy10060766 · Cited by 73 · see citing works
Highly efficient and genotype-independent barley gene editing based on anther culture
Han, Y., Broughton, S., Liu, L., Zhang, X.-Q., Zeng, J., He, X., & Li, C. (2021) · Plant Communications · doi:10.1016/j.xplc.2020.100082 · Cited by 73 · see citing works
Beyene, G., Chauhan, R. D., Wagaba, H., Moll, T., Alicai, T., Miano, D. W., Carrington, J. C., & Taylor, N. J. (2015) · Molecular Plant Pathology · doi:10.1111/mpp.12353 · Cited by 68 · see citing works
APOBEC: From mutator to editor
Yang, B., Li, X., Lei, L., & Chen, J. (2017) · Journal of genetics and genomics/Journal of Genetics and Genomics · doi:10.1016/j.jgg.2017.04.009 · Cited by 67 · see citing works
Chapman, M. A., He, Y., & Zhou, M. (2022) · New Phytologist · doi:10.1111/nph.18021 · Cited by 67 · see citing works
A simple and highly efficient Agrobacterium-mediated transformation protocol for Setaria viridis
Martins, P. K., Ribeiro, A. P., da Cunha, B. A. D. B., Kobayashi, A. K., & Molinari, H. B. C. (2015) · Biotechnology Reports · doi:10.1016/j.btre.2015.02.002 · Cited by 62 · see citing works
A new allele of the Brachytic2 gene in maize can efficiently modify plant architecture
Wei, L., Zhang, X., Zhang, Z., Liu, H., & Lin, Z. (2018) · Heredity · doi:10.1038/s41437-018-0056-3 · Cited by 59 · see citing works
Chennakesavulu, K., Singh, H., Trivedi, P. K., Jain, M., & Yadav, S. R. (2022) · Plant Cell Reports · doi:10.1007/s00299-021-02681-w · Cited by 58 · cited 1× · see citing works
“This utility of the technique also needs to be extrapolated to staple food crops (Ji et al., 2020; Chennakesavulu et al., 2021).”
BackgroundZhu, Q., Smith, S. M., Ayele, M., Yang, L., Jogi, A., Chaluvadi, S. R., & Bennetzen, J. L. (2012) · Genetics · doi:10.1534/genetics.112.144436 · Cited by 56 · see citing works
Marx, V. (2018) · Nature Methods · doi:10.1038/s41592-018-0146-4 · Cited by 55 · see citing works
In planta Genome Editing in Commercial Wheat Varieties
Liu, Y., Luo, W., Linghu, Q., Abe, F., Hisano, H., Sato, K., Kamiya, Y., Kawaura, K., Onishi, K., Endo, M., et al. (2021) · Frontiers in Plant Science · doi:10.3389/fpls.2021.648841 · Cited by 55 · see citing works
Olanrewaju, O. S., Oyatomi, O., Babalola, O. O., & Abberton, M. (2022) · Frontiers in Plant Science · doi:10.3389/fpls.2021.798993 · Cited by 55 · see citing works
Beyene, G., Chauhan, R. D., Villmer, J., Husic, N., Wang, N., Gebre, E., Girma, D., Chanyalew, S., Assefa, K., Tabor, G., et al. (2022) · Plant Biotechnology Journal · doi:10.1111/pbi.13842 · Cited by 55 · see citing works
Li, S., & Xia, L. (2019) · aBIOTECH · doi:10.1007/s42994-019-00009-7 · Cited by 53 · see citing works
Transgenic pigeonpea events expressing Cry1Ac and Cry2Aa exhibit resistance to Helicoverpa armigera
Ghosh, G., Ganguly, S., Purohit, A., Chaudhuri, R. K., Das, S., & Chakraborti, D. (2017) · Plant Cell Reports · doi:10.1007/s00299-017-2133-0 · Cited by 52 · see citing works
Sretenovic, S., Yin, D., Levav, A., Selengut, J., Mount, S. M., & Qi, Y. (2020) · Plant Communications · doi:10.1016/j.xplc.2020.100101 · Cited by 52 · see citing works
Epigenome editing: targeted manipulation of epigenetic modifications in plants
Shin, H., Choi, W. L., Lim, J. Y., & Huh, J. H. (2022) · Genes & Genomics · doi:10.1007/s13258-021-01199-5 · Cited by 51 · see citing works
Zambre, M., Goossens, A., Cardona, C., MONTAGU, M. V., Terryn, N., & Angenon, G. (2005) · Theoretical and Applied Genetics · doi:10.1007/s00122-004-1910-7 · Cited by 50 · see citing works
Saxena, R. K., Obala, J., Sinjushin, A. A., Kumar, C. V. S., Saxena, K. B., & Varshney, R. K. (2017) · Theoretical and Applied Genetics · doi:10.1007/s00122-017-2924-2 · Cited by 50 · see citing works
Houngue, J. A., Zandjanakou-Tachin, M., Ngalle, H. B., Pita, J. S., Cacaï, G. H. T., Ngatat, S., Bell, J. M., & Ahanhanzo, C. (2018) · Physiological and Molecular Plant Pathology · doi:10.1016/j.pmpp.2018.07.003 · Cited by 48 · see citing works
Monsur, M. B., Shao, G., Lv, Y., Ahmad, S., Wei, X., Hu, P., & Tang, S. (2020) · Genes · doi:10.3390/genes11040466 · Cited by 48 · see citing works
Millets: Their Unique Nutritional and Health-Promoting Attributes
TAYLOR, J. R. N. (2017) · Elsevier eBooks · doi:10.1016/b978-0-08-100866-9.00004-2 · Cited by 46 · see citing works
Translational Research: Exploring and Creating Genetic Diversity
Jacob, P., Avni, A., & Bendahmane, A. (2017) · Trends in Plant Science · doi:10.1016/j.tplants.2017.10.002 · Cited by 45 · see citing works
Bart, R. S., & Taylor, N. J. (2017) · PLoS Pathogens · doi:10.1371/journal.ppat.1006287 · Cited by 44 · see citing works
Kang, S., Jeon, S., Kim, S., Chang, Y. K., & Kim, Y. (2020) · Scientific Reports · doi:10.1038/s41598-020-78968-x · Cited by 44 · see citing works
The future of cassava in the era of biotechnology in Southern Africa
Otun, S., Escrich, A., Achilonu, I., Rauwane, M. E., Lerma-Escalera, J. A., Morones‐Ramírez, J. R., & Rios‐Solis, L. (2022) · Critical Reviews in Biotechnology · doi:10.1080/07388551.2022.2048791 · Cited by 44 · see citing works
Potential of gene drives with genome editing to increase genetic gain in livestock breeding programs
Gonen, S., Jenko, J., Gorjanc, G., Mileham, A. J., Whitelaw, B., & Hickey, J. M. (2017) · Genetics Selection Evolution · doi:10.1186/s12711-016-0280-3 · Cited by 43 · see citing works
National Research Council (1996) · National Academies Press eBooks · doi:10.17226/2305 · Cited by 43 · see citing works
Aher, R. R., Reddy, P. S., Bhunia, R. K., Flyckt, K. S., Shankhapal, A. R., Ojha, R., Everard, J. D., Wayne, L. L., Ruddy, B. M., Deonovic, B., et al. (2022) · Frontiers in Plant Science · doi:10.3389/fpls.2022.962667 · Cited by 42 · see citing works
Self-citationA modular gene targeting system for sequential transgene stacking in plants
Kumar, S., Alabed, D., Worden, A., Novak, S., Wu, H., Ausmus, C., Beck, M., Robinson, H. M., Minnicks, T., Hemingway, D., et al. (2015) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2015.04.006 · Cited by 40 · see citing works
Potential of genomics for the improvement of underutilized legumes in sub‐Saharan Africa
Paliwal, R., Adegboyega, T. T., Abberton, M. T., Faloye, B., & Oyatomi, O. (2021) · Legume Science · doi:10.1002/leg3.69 · Cited by 40 · see citing works
Modification of cereal plant architecture by genome editing to improve yields
Huang, X., Hilscher, J., Stöger, E., Christou, P., & Zhu, C. (2021) · Plant Cell Reports · doi:10.1007/s00299-021-02668-7 · Cited by 40 · see citing works
Prime Editing: Game Changer for Modifying Plant Genomes
Marzec, M., & Hensel, G. (2020) · Trends in Plant Science · doi:10.1016/j.tplants.2020.05.008 · Cited by 39 · see citing works
Plant epigenomics for extenuation of abiotic stresses: challenges and future perspectives
Singh, D., Chaudhary, P., Taunk, J., Singh, C. K., Sharma, S. R., Singh, V. J., Singh, D., Chinnusamy, V., Yadav, R., & Pal, M. (2021) · Journal of Experimental Botany · doi:10.1093/jxb/erab337 · Cited by 39 · see citing works
Cui, X., Balcerzak, M., Schernthaner, J. P., Babic, V., Datla, R., Brauer, E. K., Labbé, N., Subramaniam, R., & Ouellet, T. (2019) · Plant Methods · doi:10.1186/s13007-019-0500-2 · Cited by 38 · see citing works
Genome-editing technologies: the gap between application and policy
Li, G., Liu, Y., & Chen, Y. (2019) · Science China Life Sciences · doi:10.1007/s11427-019-1566-1 · Cited by 38 · see citing works
A short overview of CRISPR-Cas technology and its application in viral disease control
Ghorbani, A., Hadifar, S., Salari, R., Izadpanah, K., Burmistrz, M., Afsharifar, A., Eskandari, M. H., Niazi, A., Denes, C. E., & Neely, G. G. (2021) · Transgenic Research · doi:10.1007/s11248-021-00247-w · Cited by 38 · see citing works
Achieving Plant Genome Editing While Bypassing Tissue Culture
Ji, X., Yang, B., & Wang, D. (2020) · Trends in Plant Science · doi:10.1016/j.tplants.2020.02.011 · Cited by 36 · see citing works
Harnessing genetic resources and progress in plant genomics for fonio (Digitaria spp.) improvement
Ayenan, M. A. T., Sodedji, K. A. F., Nwankwo, C. I., Olodo, K. F., & Alladassi, B. M. E. (2017) · Genetic Resources and Crop Evolution · doi:10.1007/s10722-017-0565-6 · Cited by 32 · see citing works
An improved protocol for efficient transformation and regeneration of Setaria italica
Santos, C., Romeiro, D., Silva, J. P. S., Basso, M. F., Molinari, H. B. C., & Centeno, D. C. (2020) · Plant Cell Reports · doi:10.1007/s00299-019-02505-y · Cited by 31 · see citing works
Gene tagging via CRISPR-mediated homology-directed repair in cassava
Veley, K. M., Okwuonu, I. C., Jensen, G., Yoder, M., Taylor, N. J., Meyers, B. C., & Bart, R. S. (2021) · G3 Genes Genomes Genetics · doi:10.1093/g3journal/jkab028 · Cited by 31 · see citing works
Minnaar‐Ontong, A., Gerrano, A. S., & Labuschagne, M. T. (2021) · Scientific Reports · doi:10.1038/s41598-021-86977-7 · Cited by 30 · see citing works
Genome editing for crop improvement: A perspective from India
Bhattacharya, A., Parkhi, V., & Char, B. R. (2021) · In Vitro Cellular & Developmental Biology - Plant · doi:10.1007/s11627-021-10184-2 · Cited by 28 · see citing works
Genomics for greater efficiency in pigeonpea hybrid breeding
Saxena, R. K., Saxena, K. B., Pazhamala, L. T., Patel, K., Parupalli, S., Sameerkumar, C. V., & Varshney, R. K. (2015) · Frontiers in Plant Science · doi:10.3389/fpls.2015.00793 · Cited by 27 · see citing works
A piggyBac‐mediated transgenesis system for the temporary expression of CRISPR/Cas9 in rice
Nishizawa‐Yokoi, A., & Toki, S. (2021) · Plant Biotechnology Journal · doi:10.1111/pbi.13559 · Cited by 27 · see citing works
Numan, M., Khan, A. L., Asaf, S., Salehin, M., Beyene, G., Tadele, Z., & Ligaba‐Osena, A. (2021) · Plants · doi:10.3390/plants10040628 · Cited by 26 · see citing works
Pigeonpea (Cajanus cajan L. Millsp.)
Sharma, K. K., Sreelatha, G., & Dayal, S. (2006) · Humana Press eBooks · doi:10.1385/1-59745-130-4:359 · Cited by 25 · see citing works
Self-citationVirus-Mediated Genome Editing in Plants Using the CRISPR/Cas9 System
Mahas, A., Ali, Z., Tashkandi, M., & Mahfouz, M. M. (2019) · Methods in molecular biology · doi:10.1007/978-1-4939-8991-1_23 · Cited by 24 · see citing works
Bambara Groundnut [Vigna subterranea (L.) Verdc. (Fabaceae)] Usage in Human Health
Koné, M., Paice, A. G., & Touré, Y. (2011) · Nuts and Seeds in Health and Disease Prevention · doi:10.1016/b978-0-12-375688-6.10022-2 · Cited by 21 · see citing works
Use of CRISPR/Cas9 for Targeted Mutagenesis in Sorghum
Char, S. N., Lee, H., & Yang, B. (2020) · Current Protocols in Plant Biology · doi:10.1002/cppb.20112 · Cited by 21 · see citing works
Nelson‐Vasilchik, K., Hague, J. P., Tilelli, M., & Kausch, A. P. (2022) · In Vitro Cellular & Developmental Biology - Plant · doi:10.1007/s11627-021-10243-8 · Cited by 21 · see citing works
Agrobacterium: A Genome-Editing Tool-Delivery System
Sardesai, N., & Subramanyam, S. (2018) · Current topics in microbiology and immunology · doi:10.1007/82_2018_101 · Cited by 20 · see citing works
Climate smart crops for food and nutritional security for semi-arid zones of Zimbabwe
Jiri, O., Mafongoya, P., & Chivenge, P. P. (2017) · African Journal of Food Agriculture Nutrition and Development · doi:10.18697/ajfand.79.16285 · Cited by 19 · see citing works
Ramineni, R., Sadumpati, V., Khareedu, V. R., & Vudem, D. R. (2014) · PLoS ONE · doi:10.1371/journal.pone.0090839 · Cited by 18 · see citing works
Tribhuvan, K. U., Das, A., Srivastava, H., Kumar, K., Durgesh, K., Sandhya, S., Mithra, S., Jain, P. K., & Gaikwad, K. (2020) · 3 Biotech · doi:10.1007/s13205-020-02180-x · Cited by 18 · see citing works
Flagged first, then by citations
Research works printed but not linked: 2
- Rojas-Vasquez R. Gatica-Arias A. (2020). Use of genome editing technologies for genetic improvement of crops of tropical origin. Plant Cell, Tissue and Organ Culture (PCTOC) 140 (1), 215– 244. 10.1111/pbi.13635 · DOI · Paper page
- Sharma B. (2015). Biochemical characterization of pearl millet (Pennisetum glaucum (L.) R. Br.) genotypes for identifying off-odour factors. Hisar, India: CCS Haryana Agricultural University. Ph.D. Thesis.
Cited by
Sorted by citations
Genome editing in food and agriculture: from regulations to consumer perspectives
Atimango, A. O., Wesana, J., Kalule, S. W., Verbeke, W., & Steur, H. D. (2024) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2024.103127 · Cited by 48 · see citing works
Emerging applications of gene editing technologies for the development of climate-resilient crops
Chavhan, R. L., Jaybhaye, S. G., Hinge, V. R., Deshmukh, A. S., Shaikh, U. S., Jadhav, P. K., Kadam, U. S., & Hong, J. C. (2025) · Frontiers in Genome Editing · doi:10.3389/fgeed.2025.1524767 · Cited by 43 · see citing works
Current trends and prospects in quinoa research: An approach for strategic knowledge areas
Martínez, D. H. F., Cortina, J. R., Chavez‐Oliveros, L. F., Aguilera‐Arango, G. A., & Castañeda, A. M. (2023) · Food Science & Nutrition · doi:10.1002/fsn3.3891 · Cited by 26 · see citing works
Chawla, R., Poonia, A., Samantara, K., Mohapatra, S. R., Naik, S. B., Ashwath, M. N., Djalović, I. G., & Prasad, P. V. V. (2023) · Frontiers in Genetics · doi:10.3389/fgene.2023.1204585 · Cited by 23 · see citing works
Devkota, K. P., Devkota, M., Mabhaudhi, T., Nangia, V., Attaher, S. M., Boroto, R. J., Timsina, J., & Siddique, K. H. M. (2025) · Food and Energy Security · doi:10.1002/fes3.70046 · Cited by 16 · see citing works
Tahakik, R., Deshmukh, A. G., Moharil, M. P., Jadhav, P. V., Kogade, V., More, K. D., & Shinde, V. P. (2024) · Bulletin of the National Research Centre/Bulletin of the National Research Center · doi:10.1186/s42269-024-01281-4 · Cited by 11 · see citing works
Millet biofortification for enhanced iron content: Roadmap for combating hidden hunger
Chaudhary, R., Sharma, C., Kumar, V., Rajput, V. S., Naik, B., Prasad, R., Sharma, S., & Kumar, V. (2025) · Journal of Agriculture and Food Research · doi:10.1016/j.jafr.2025.101654 · Cited by 11 · see citing works
Gelaye, Y., & Luo, H. (2025) · Crop Science · doi:10.1002/csc2.70145 · Cited by 10 · see citing works
Advancements in Genetic Enhancement: CRISPR/Cas-Mediated Genome Editing in Leguminous Crops
Roy, A., & Sandhu, R. (2024) · Journal of Advances in Biology & Biotechnology · doi:10.9734/jabb/2024/v27i6927 · Cited by 9 · see citing works
Wang, Y., Tang, Q., Kang, Y., Wang, X., Zhang, H., & Li, X. (2022) · Cells · doi:10.3390/cells11213471 · Cited by 9 · see citing works
Gene editing tool kit in millets: present status and future directions
Sapara, V., Khisti, M., Yogendra, K. N., & Reddy, P. S. (2024) · The Nucleus · doi:10.1007/s13237-024-00485-3 · Cited by 8 · see citing works
Steps to transform African opportunity crops into reality crops
Rutsaert, P., Achigan-Dako, E. G., Ayenan, M. A. T., Chamberlin, J., Daudi, H., Houdegbe, A. C., Kresovich, S., Mbugua-Gitonga, A., Nas, T. M. S., Ojiewo, C. O., et al. (2026) · Nature Communications · doi:10.1038/s41467-026-70990-3 · Cited by 5 · see citing works
Aher, R. R., & Reddy, P. S. (2025) · Journal of Plant Biochemistry and Biotechnology · doi:10.1007/s13562-025-00982-9 · Cited by 5 · see citing works
Bu, G., Ma, X., Jin, H., & Wang, L. (2025) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2025.144785 · Cited by 4 · see citing works
RetractedMapping the plant genome: CRISPR/Cas9 as a toolkit for functional genomics
Thabet, S. G., Elkelish, A., Dawood, M. F. A., Safhi, F. A., Alhudhaibi, A. M., Börner, A., & Alqudah, A. M. (2025) · Acta Physiologiae Plantarum · doi:10.1007/s11738-025-03872-8 · Cited by 4 · see citing works
Swain, S., Debnath, S., Khamassi, K., Samantara, K., Ram, P., Kumar, A., Mahawer, A. K., & Kaul, T. (2025) · Plant Gene · doi:10.1016/j.plgene.2025.100534 · Cited by 3 · see citing works
Mazumder, S., Bhattacharya, D., Lahiri, D., & Nag, M. (2026) · Journal of Crop Science and Biotechnology · doi:10.1007/s12892-025-00337-9 · Cited by 1 · see citing works
Kavitha, N. K. R., Suyambu, R., Parthiban, P., Balasubramani, G., & Pandian, A. (2026) · Journal of environmental chemical engineering · doi:10.1016/j.jece.2026.122389 · Cited by 1 · see citing works
Crop wild relatives of legumes: evolutionary resources for climate-responsive pre-breeding
Avasiloaiei, D. I., Calara, M., Brezeanu, P. M., Burzo, I., & Brezeanu, C. (2026) · Frontiers in Plant Science · doi:10.3389/fpls.2026.1892793 · Cited by 1 · see citing works
Current status and development trend of cassava breeding
Chen, S., Cai, J., An, F., Zhu, W., Luo, X., Xue, J., Xue, M., HanFeng, L. I., Wei, Z., Huang, S., et al. (2024) · Scientia Sinica Vitae · doi:10.1360/ssv-2024-0256 · Cited by 1 · see citing works
Application of Genome Editing Technologies to Improve Grain Quality of Pulses
Khezri, M., Zakaria, R. A., & Zare, N. (2025) · Sustainable landscape planning and natural resources management · doi:10.1007/978-3-031-81639-0_14
Waktola, M., & Dabalo, A. (2025) · Journal of Plant Sciences · doi:10.11648/j.jps.20251303.16
Resistance of Common Bean (Phaseolus vulgaris L.) Varieties to Bruchid Infestation in Burundi
Nsabimana, A., Shechambo, L. F., & Lyimo, P. J. (2025) · Journal of Current Opinion in Crop Science · doi:10.62773/jcocs.v6i4.351
ADVANCES IN TISSUE CULTURE FOR LEGUME IMPROVEMENT
Rawte, S., Jha, Z., Sahu, H. K., Nandedkar, K., & Xalxo, M. S. (2026) · Genetics and Molecular Research · doi:10.4238/b1k7wy73
Appraising the potential of gene editing in Zimbabwe’s crop improvement programmes: a review
Mativavarira, M., ZİMUTO, T., Chingwaru, W., Manhokwe, S., Chitsiku, R. L., Murashiki, T. C., Bhusvumani, S., Njanji, R., Mubani, M. A., Chirilele, P., et al. (2026) · Cogent Food & Agriculture · doi:10.1080/23311932.2026.2719964
Current status and challenges of food biotechnology in Africa
Mmbando, G. S. (2026) · Discover Biotechnology · doi:10.1007/s44340-026-00063-0
- Authors
- Sharma, K. K., Palakolanu, S. R., Bhattacharya, J., Shankhapal, A. R., & Bhatnagar-Mathur, P.
- Affiliations
- Energy and Resources Institute (IN)
- International Crops Research Institute for the Semi-Arid Tropics (IN)
- University of Nottingham (GB)
- Corresponding author
- Sharma, Kiran K.
- Venue
- Frontiers in Genetics
- Publisher
- Frontiers Media SA
- Published
- 2022-10-06
- Volume
- 13
- Pages
- 999207-999207
- ISSN
- 1664-8021 (ISSN-L)
- PMC
- PMC9582247
- OpenAlex
- W4303192285
- Cited by
- 24
- References
- 209
- Citations by year
- 2026: 6 · 2025: 10 · 2024: 5 · 2023: 2 · 2022: 1
- FWCI
- 0.47
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Frontiers in Genetics (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Open Access Repository of ICRISAT (International Crops Research Institute for the Semi-Arid Tropics) (acceptedVersion)
- Rothamsted Repository (Rothamsted Repository) (acceptedVersion, other-oa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Consortium of International Agricultural Research Centers
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Agricultural Productivity and Crop Improvement
- Keywords
- CRISPR/Cas genome editing, rainfed agriculture, genetic gain, base editing, prime editing, multiplex genome editing, millet, Eragrostis tef, fonio, quinoa, Vigna subterranea, Cajanus cajan, cassava, stress tolerance, nutritional improvement, yield improvement, food security
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review