Cited by 507Pluripotent Stem Cells ResearchNeurogenesis and neuroplasticity mechanismsDevelopmental Biology and Gene Regulation

The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.

Reliability of human cortical organoid generation

Open access

Yoon, Se-Jin, Elahi, Lubayna S., Pașca, Anca M., Marton, Rebecca M., Gordon, Aaron, Revah, Omer, Miura, Yuki, Walczak, Elisabeth M., Holdgate, Gwendolyn M., Fan, H. Christina, Huguenard, John R., Geschwind, Daniel H., Pașca, Sergiu P.·2019·Nature Methods

DOI 10.1038/s41592-018-0255-0PMC PMC6677388

Open this paper in CoChat — free account

Abstract

The differentiation of pluripotent stem cells in three-dimensional cultures can recapitulate key aspects of brain development, but protocols are prone to variable results. Here we differentiated multiple human pluripotent stem cell lines for over 100 d using our previously developed approach to generate brain-region-specific organoids called cortical spheroids and, using several assays, found that spheroid generation was highly reliable and consistent. We anticipate the use of this approach for large-scale differentiation experiments and disease modeling.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1038/s41592-018-0255-0

Cites

29 of 29 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

STAR: ultrafast universal RNA-seq aligner
Dobin, A., Davis, C. A., Schlesinger, F., Drenkow, J., Zaleski, C., Jha, S., Batut, P., Chaisson, M., & Gingeras, T. R. (2013) · Bioinformatics · doi:10.1093/bioinformatics/bts635 · Cited by 51,709
<tt>edgeR</tt> : a Bioconductor package for differential expression analysis of digital gene expression data
Robinson, M. D., McCarthy, D. J., & Smyth, G. K. (2010) · Bioinformatics · doi:10.1093/bioinformatics/btp616 · Cited by 41,364
limma powers differential expression analyses for RNA-sequencing and microarray studies
Ritchie, M. E., Phipson, B., Wu, D., Hu, Y., Law, C. W., Shi, W., & Smyth, G. K. (2015) · Nucleic Acids Research · doi:10.1093/nar/gkv007 · Cited by 40,458
PLINK: a tool set for whole-genome association and population-based linkage analyses.
Purcell, S., Neale, B., Todd-Brown, K., Thomas, L., Ferreira, M. A. R., Bender, D., Maller, J., Sklar, P., de Bakker, P. I. W., Daly, M. J., et al. (2007) · The American Journal of Human Genetics · doi:10.1086/519795 · Cited by 34,089
The Genome Analysis Toolkit: A MapReduce framework for analyzing next-generation DNA sequencing data
McKenna, A., Hanna, M., Banks, E., Sivachenko, A., Cibulskis, K., Kernytsky, A., Garimella, K., Altshuler, D., Gabriel, S., Daly, M., et al. (2010) · Genome Research · doi:10.1101/gr.107524.110 · Cited by 27,895
featureCounts: an efficient general purpose program for assigning sequence reads to genomic features
Liao, Y., Smyth, G. K., & Shi, W. (2014) · Bioinformatics · doi:10.1093/bioinformatics/btt656 · Cited by 27,219
RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome
Li, B., & Dewey, C. N. (2011) · BMC Bioinformatics · doi:10.1186/1471-2105-12-323 · Cited by 22,179
Adjusting batch effects in microarray expression data using empirical Bayes methods
Johnson, W. E., Li, C., & Rabinovic, A. (2007) · Biostatistics · doi:10.1093/biostatistics/kxj037 · Cited by 8,658
Cerebral organoids model human brain development and microcephaly
Lancaster, M. A., Renner, M., Martin, C.-A., Wenzel, D., Bicknell, L. S., Hurles, M. E., Homfray, T., Penninger, J. M., Jackson, A. P., & Knoblich, J. A. (2013) · Nature · doi:10.1038/nature12517 · Cited by 5,372
Brain-Region-Specific Organoids Using Mini-bioreactors for Modeling ZIKV Exposure
Qian, X., Nguyen, H. N., Song, M. M., Hadiono, C., Ogden, S. C., Hammack, C., Yao, B., Hamersky, G. R., Jacob, F., Zhong, C., et al. (2016) · Cell · doi:10.1016/j.cell.2016.04.032 · Cited by 2,127

Show all 28 references

Sorted by citations

Items 101–200 of 491

Brain organoid: a 3D technology for investigating cellular composition and interactions in human neurological development and disease models in vitro
Agboola, O. S., Hu, X., Shan, Z., Wu, Y., & Lei, L. (2021) · Stem Cell Research & Therapy · doi:10.1186/s13287-021-02369-8 · Cited by 53
Modeling human neurodevelopmental diseases with brain organoids
Lu, X., Yang, J., & Xiang, Y. (2022) · Cell Regeneration · doi:10.1186/s13619-021-00103-6 · Cited by 50
Bilirubin-Induced Neurological Damage: Current and Emerging iPSC-Derived Brain Organoid Models
Pranty-Weßelmann, A. I., Shumka, S., & Adjaye, J. (2022) · Cells · doi:10.3390/cells11172647 · Cited by 48
Transcriptional and functional effects of lithium in bipolar disorder iPSC-derived cortical spheroids
Osete, J. R., Akkouh, I. A., Ievglevskyi, O., Vandenberghe, M., de Assis, D. R., Ueland, T., Kondratskaya, E., Holen, B., Szabó, A., Hughes, T., et al. (2023) · Molecular Psychiatry · doi:10.1038/s41380-023-01944-0 · Cited by 52
From Brain Organoids to Networking Assembloids: Implications for Neuroendocrinology and Stress Medicine
Makrygianni, E. A., & Chrousos, G. P. (2021) · Frontiers in Physiology · doi:10.3389/fphys.2021.621970 · Cited by 49
Modeling the complex genetic architectures of brain disease
Fernando, M. B., Ahfeldt, T., & Brennand, K. (2020) · Nature Genetics · doi:10.1038/s41588-020-0596-3 · Cited by 48
Current progress of cerebral organoids for modeling Alzheimer's disease origins and mechanisms
Sreenivasamurthy, S., Laul, M., Zhao, N., Kim, T., & Zhu, D. (2022) · Bioengineering & Translational Medicine · doi:10.1002/btm2.10378 · Cited by 49
Human tau mutations in cerebral organoids induce a progressive dyshomeostasis of cholesterol
Glasauer, S. M. K., Goderie, S. K., Rauch, J. N., Guzman, E., Audouard, M., Bertucci, T., Joy, S., Rommelfanger, E., Luna, G., Keane-Rivera, E., et al. (2022) · Stem Cell Reports · doi:10.1016/j.stemcr.2022.07.011 · Cited by 46
Microglia-containing cerebral organoids derived from induced pluripotent stem cells for the study of neurological diseases
Hong, Y., Dong, X., Chang, L., Xie, C., Chang, M., Aguilar, J. S., Lin, J., Lin, J., & Li, Q. Q. (2023) · iScience · doi:10.1016/j.isci.2023.106267 · Cited by 46
Mutant huntingtin impairs neurodevelopment in human brain organoids through CHCHD2-mediated neurometabolic failure
Lisowski, P., Lickfett, S., Rybak‐Wolf, A., Menacho, C., Le, S., Pentimalli, T. M., Notopoulou, S., Dykstra, W., Oehler, D., López-Calcerrada, S., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-51216-w · Cited by 51
Patient-Derived Midbrain Organoids to Explore the Molecular Basis of Parkinson's Disease
Galet, B., Cheval, H., & Ravassard, P. (2020) · Frontiers in Neurology · doi:10.3389/fneur.2020.01005 · Cited by 47
Murine cerebral organoids develop network of functional neurons and hippocampal brain region identity
Ciarpella, F., Zamfir, R. G., Campanelli, A., Ren, E., Pedrotti, G., Bottani, E., Borioli, A., Caron, D., Chio, M. D., Dolci, S., et al. (2021) · iScience · doi:10.1016/j.isci.2021.103438 · Cited by 47
Brain organoids: A promising model to assess oxidative stress‐induced central nervous system damage
Oyefeso, F. A., Muotri, A. R., Wilson, C. G., & Pecaut, M. J. (2021) · Developmental Neurobiology · doi:10.1002/dneu.22828 · Cited by 44
Patterning of brain organoids derived from human pluripotent stem cells
Zhang, Z., O’Laughlin, R., Song, H., & Ming, G. (2022) · Current Opinion in Neurobiology · doi:10.1016/j.conb.2022.102536 · Cited by 45
Toward the next generation of vascularized human neural organoids
Li, M., Gao, L., Zhao, L., Zou, T., & Xu, H. (2022) · Medicinal Research Reviews · doi:10.1002/med.21922 · Cited by 45
TGFβ superfamily signaling regulates the state of human stem cell pluripotency and capacity to create well-structured telencephalic organoids
Watanabe, M., Buth, J. E., Haney, J. R., Vishlaghi, N., Turcios, F., Elahi, L. S., Gu, W., Pearson, C. A., Kurdian, A., Baliaouri, N. V., et al. (2022) · Stem Cell Reports · doi:10.1016/j.stemcr.2022.08.013 · Cited by 45
Self-citation
Large‐Scale Production of Wholly Cellular Bioinks via the Optimization of Human Induced Pluripotent Stem Cell Aggregate Culture in Automated Bioreactors
Ho, D. L. L., Lee, S., Du, J., Weiss, J. D., Tam, T., Sinha, S., Klinger, D., Devine, S., Hamfeldt, A., Leng, H. T., et al. (2022) · Advanced Healthcare Materials · doi:10.1002/adhm.202201138 · Cited by 44
Stem Cell–Based Organoid Models of Neurodevelopmental Disorders
Wang, L., Owusu-Hammond, C., Sievert, D., & Gleeson, J. G. (2023) · Biological Psychiatry · doi:10.1016/j.biopsych.2023.01.012 · Cited by 44
Probing disrupted neurodevelopment in autism using human stem cell‐derived neurons and organoids: An outlook into future diagnostics and drug development
Yang, G., & Shcheglovitov, A. (2019) · Developmental Dynamics · doi:10.1002/dvdy.100 · Cited by 46
SCN2A channelopathies in the autism spectrum of neuropsychiatric disorders: a role for pluripotent stem cells?
Kruth, K., Grisolano, T. M., Ahern, C. A., & Williams, A. J. (2020) · Molecular Autism · doi:10.1186/s13229-020-00330-9 · Cited by 45
Exploiting CRISPR Cas9 in Three-Dimensional Stem Cell Cultures to Model Disease
Gopal, S., Rodrigues, A. L., & Dordick, J. S. (2020) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2020.00692 · Cited by 46
BrainPhys neuronal medium optimized for imaging and optogenetics in vitro
Zabolocki, M., McCormack, K., van den Hurk, M., Milky, B., Shoubridge, A. P., Adams, R., Tran, J., Mahadevan-Jansen, A. M., Reineck, P., Thomas, J., et al. (2020) · Nature Communications · doi:10.1038/s41467-020-19275-x · Cited by 45
Spin∞: an updated miniaturized spinning bioreactor design for the generation of human cerebral organoids from pluripotent stem cells
Romero-Morales, A. I., O’Grady, B. J., Balotin, K. M., Bellan, L. M., Lippmann, E. S., & Gama, V. (2019) · HardwareX · doi:10.1016/j.ohx.2019.e00084 · Cited by 45
Human in vitro models for understanding mechanisms of autism spectrum disorder
Gordon, A., & Geschwind, D. H. (2020) · Molecular Autism · doi:10.1186/s13229-020-00332-7 · Cited by 45
Self-citation
Organoid and microfluidics-based platforms for drug screening in COVID-19
Ramezankhani, R., Solhi, R., Chai, Y. C., Vosough, M., & Verfaillie, C. M. (2021) · Drug Discovery Today · doi:10.1016/j.drudis.2021.12.014 · Cited by 46
The potential of in vitro neuronal networks cultured on micro electrode arrays for biomedical research
Cerina, M., Piastra, M. C., & Frega, M. (2023) · Progress in Biomedical Engineering · doi:10.1088/2516-1091/acce12 · Cited by 44
Incorporation of stem cell-derived astrocytes into neuronal organoids to allow neuro-glial interactions in toxicological studies
Brüll, M. (2020) · ALTEX · doi:10.14573/altex.1911111 · Cited by 44
Single-cell long-read sequencing in human cerebral organoids uncovers cell-type-specific and autism-associated exons
Yang, Y., Yang, R., Kang, B., Qian, S., He, X., & Zhang, X. (2023) · Cell Reports · doi:10.1016/j.celrep.2023.113335 · Cited by 41
Biofunctionalized gelatin hydrogels support development and maturation of iPSC-derived cortical organoids
Kjar, A., Haschert, M. R., Zepeda, J. C., Simmons, A. J., Yates, A., Chavarría, D., Fernandez, M., Robertson, G. L., Abdulrahman, A. M., Kim, H., et al. (2024) · Cell Reports · doi:10.1016/j.celrep.2024.114874 · Cited by 43
Developmental convergence and divergence in human stem cell models of autism
Gordon, A., Yoon, S., Bicks, L. K., Martín, J. M., Pintacuda, G., Arteaga, S. A., Wamsley, B., Guo, Q., Elahi, L. S., Dolmetsch, R. E., et al. (2026) · Nature · doi:10.1038/s41586-025-10047-5 · Cited by 42
Self-citation
Generation of Regionally Specified Human Brain Organoids Resembling Thalamus Development
Xiang, Y., Çakır, B., & Park, I. (2020) · STAR Protocols · doi:10.1016/j.xpro.2019.100001 · Cited by 41
Bioengineering tissue morphogenesis and function in human neural organoids
Fedorchak, N. J., Iyer, N. R., & Ashton, R. S. (2020) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2020.05.025 · Cited by 41
Methylene blue is a potent and broad-spectrum inhibitor against Zika virus in vitro and in vivo
Li, Z., Lang, Y., Sakamuru, S., Samrat, S. K., Trudeau, N., Kuo, L., Rugenstein, N., Tharappel, A. M., D’Brant, L., Koetzner, C. A., et al. (2020) · Emerging Microbes & Infections · doi:10.1080/22221751.2020.1838954 · Cited by 41
Methadone interrupts neural growth and function in human cortical organoids
Yao, H., Wu, W., Cerf, I., Zhao, H. W., Wang, J., Negraes, P. D., Muotri, A. R., & Haddad, G. G. (2020) · Stem Cell Research · doi:10.1016/j.scr.2020.102065 · Cited by 41
Recent Trends and Perspectives in Cerebral Organoids Imaging and Analysis
Martin, C. B., Simon-Chane, C., Clouchoux, C., & Histace, A. (2021) · Frontiers in Neuroscience · doi:10.3389/fnins.2021.629067 · Cited by 41
Novel Thermosensitive Hydrogel Promotes Spinal Cord Repair by Regulating Mitochondrial Function
Li, Y., Yang, L., Hu, F., Xu, J., Ye, J., Liu, S., Wang, L., Zhuo, M., Ran, B., Zhang, H., et al. (2022) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.2c04341 · Cited by 40
Challenges and opportunities for precision medicine in neurodevelopmental disorders
Chen, G. T., & Geschwind, D. H. (2022) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2022.114564 · Cited by 40
Self-citation
Microfluidic Brain‐on‐a‐Chip: From Key Technology to System Integration and Application
Wang, Z., Zhang, Y., Li, Z., Wang, H., Li, N., & Deng, Y. (2023) · Small · doi:10.1002/smll.202304427 · Cited by 41
Potassium ion channel modulation at cancer-neural interface enhances neuronal excitability in epileptogenic glioblastoma multiforme
Zhang, Y., Duan, W., Chen, L., Chen, J., Xu, W., Fan, Q., Li, S., Liu, Y., Wang, S., He, Q., et al. (2024) · Neuron · doi:10.1016/j.neuron.2024.10.016 · Cited by 44
Simplified Brain Organoids for Rapid and Robust Modeling of Brain Disease
Ha, J., Kang, J. S., Lee, M., Baek, A., Kim, S., Chung, S., Lee, M., & Kim, J. (2020) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2020.594090 · Cited by 40
What Have Organoids and Assembloids Taught Us About the Pathophysiology of Neuropsychiatric Disorders?
Levy, R. J., & Pașca, S. P. (2022) · Biological Psychiatry · doi:10.1016/j.biopsych.2022.11.017 · Cited by 45
Self-citation
GTF2I dosage regulates neuronal differentiation and social behavior in 7q11.23 neurodevelopmental disorders
Tobon, A., Shyti, R., Villa, C. E., Cheroni, C., Fuentes-Bravo, P., Trattaro, S., Caporale, N., Troglio, F., Tenderini, E., Mihailovich, M., et al. (2023) · Science Advances · doi:10.1126/sciadv.adh2726 · Cited by 40
Organoid and pluripotent stem cells in Parkinson’s disease modeling: an expert view on their value to drug discovery
Marotta, N., Kim, S., & Krainc, D. (2020) · Expert Opinion on Drug Discovery · doi:10.1080/17460441.2020.1703671 · Cited by 40
Maternal Distress and Offspring Neurodevelopment: Challenges and Opportunities for Pre-clinical Research Models
Fitzgerald, E., Parent, C. I., Kee, M. Z. L., & Meaney, M. J. (2021) · Frontiers in Human Neuroscience · doi:10.3389/fnhum.2021.635304 · Cited by 41
Harnessing cerebral organoids for Alzheimer's disease research
Bubnys, A., & Tsai, L. (2021) · Current Opinion in Neurobiology · doi:10.1016/j.conb.2021.10.003 · Cited by 41
Increased Tau Expression Correlates with Neuronal Maturation in the Developing Human Cerebral Cortex
Fiock, K. L., Smalley, M. E., Crary, J. F., Pașca, A. M., & Hefti, M. M. (2020) · eNeuro · doi:10.1523/eneuro.0058-20.2020 · Cited by 41
Self-citation
Deconstructing and reconstructing the human brain with regionally specified brain organoids
Xiang, Y., Çakır, B., & Park, I. (2020) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2020.05.023 · Cited by 39
Benchmarking brain organoid recapitulation of fetal corticogenesis
Cheroni, C., Trattaro, S., Caporale, N., Tobon, A., Tenderini, E., Sebastiani, S., Troglio, F., Gabriele, M., Bressan, R. B., Pollard, S. M., et al. (2022) · Translational Psychiatry · doi:10.1038/s41398-022-02279-0 · Cited by 43
New frontiers in modeling tuberous sclerosis with human stem cell‐derived neurons and brain organoids
Blair, J. D., & Bateup, H. S. (2019) · Developmental Dynamics · doi:10.1002/dvdy.60 · Cited by 37
Optogenetics in the Era of Cerebral Organoids
Shiri, Z., Simorgh, S., Naderi, S., & Baharvand, H. (2019) · Trends in biotechnology · doi:10.1016/j.tibtech.2019.05.009 · Cited by 37
Looking at neurodevelopment through a big data lens
Briscoe, J., & Marín, Ó. (2020) · Science · doi:10.1126/science.aaz8627 · Cited by 38
The Future of 3D Brain Cultures in Developmental Neurotoxicity Testing
Högberg, H. T., & Smirnova, L. (2022) · Frontiers in Toxicology · doi:10.3389/ftox.2022.808620 · Cited by 38
Multiplexing cortical brain organoids for the longitudinal dissection of developmental traits at single-cell resolution
Caporale, N., Castaldi, D., Rigoli, M. T., Cheroni, C., Valenti, A., Stucchi, S., Lessi, M., Bulgheresi, D., Trattaro, S., Pezzali, M., et al. (2024) · Nature Methods · doi:10.1038/s41592-024-02555-5 · Cited by 37
Organoid-based tissue engineering for advanced tissue repair and reconstruction
Hsiung, N., Ju, Y., Yang, K. C., Pu, Y., Zeng, W., Zhao, H., Zou, P., Ye, J., Yi, K., & Wang, X. (2025) · Materials Today Bio · doi:10.1016/j.mtbio.2025.102093 · Cited by 40
Reverse engineering human brain evolution using organoid models
Mostajo-Radji, M. A., Schmitz, M. T., Torres, S., & Pollen, A. A. (2019) · Brain Research · doi:10.1016/j.brainres.2019.146582 · Cited by 36
Biomaterial-minimalistic photoactivated bioprinting of cell-dense tissues
Wang, M., Li, W., Hao, J., Cai, L., Mei, X., Flores, R. S., Castillo, P., Garciamendez‐Mijares, C. E., Mu, X., Kuang, X., et al. (2025) · Cell · doi:10.1016/j.cell.2025.11.012 · Cited by 34
Human brain development through the lens of cerebral organoid models
Andrews, M. G., & Nowakowski, T. J. (2019) · Brain Research · doi:10.1016/j.brainres.2019.146470 · Cited by 35
Building the brain from scratch: Engineering region-specific brain organoids from human stem cells to study neural development and disease
Jacob, F., Schnoll, J. G., Song, H., & Ming, G. (2021) · Current topics in developmental biology/Current Topics in Developmental Biology · doi:10.1016/bs.ctdb.2020.12.011 · Cited by 34
AAV vectors trigger DNA damage responses and STING-dependent inflammation in human CNS cells
Costa-Verdera, H., Meneghini, V., Fitzpatrick, Z., Alezz, M. A., Fabyanic, E., Huang, X., Dzhashiashvili, Y., Ahiya, A., Mangiameli, E., Valeri, E., et al. (2024) · doi:10.21203/rs.3.rs-4171795/v1 · Cited by 32
AAV vectors trigger DNA damage response-dependent pro-inflammatory signalling in human iPSC-derived CNS models and mouse brain
Costa-Verdera, H., Meneghini, V., Fitzpatrick, Z., Abou Alezz, M., Fabyanic, E., Huang, X., Dzhashiashvili, Y., Ahiya, A., Mangiameli, E., Valeri, E., et al. (2025) · Nature Communications · doi:10.1038/s41467-025-58778-3 · Cited by 32
Seq-ing Higher Ground: Functional Investigation of Adaptive Variation Associated With High-Altitude Adaptation
Hall, J. E., Lawrence, E. S., Simonson, T. S., & Fox, K. (2020) · Frontiers in Genetics · doi:10.3389/fgene.2020.00471 · Cited by 32
Design Approaches for Generating Organ Constructs
Xia, Y. F., & Belmonte, J. C. I. (2019) · Cell stem cell · doi:10.1016/j.stem.2019.05.016 · Cited by 32
Multistructured hydrogel promotes nerve regeneration
Zhu, N., Zhuang, Y., Sun, W., Wang, J., Wang, F., Han, X., Han, Z., Ni, M., Cui, W., & Qiu, Y. (2024) · Materials Today Advances · doi:10.1016/j.mtadv.2024.100465 · Cited by 31
Modeling traumatic brain injury with human brain organoids
Jgamadze, D., Johnson, V. E., Wolf, J. A., Cullen, D. K., Song, H., Ming, G., Smith, D. H., & Chen, H.-C. I. (2020) · Current Opinion in Biomedical Engineering · doi:10.1016/j.cobme.2020.05.004 · Cited by 33
Resolving Neurodevelopmental and Vision Disorders Using Organoid Single-Cell Multi-omics
Brancati, G., Treutlein, B., & Camp, J. G. (2020) · Neuron · doi:10.1016/j.neuron.2020.09.001 · Cited by 32
Cross-site reproducibility of human cortical organoids reveals consistent cell type composition and architecture
Glass, M. R., Waxman, E. A., Yamashita, S., Lafferty, M. J., Beltran, A. A., Farah, T. M., Patel, N. K., Singla, R., Matoba, N., Ahmed, S. M., et al. (2024) · Stem Cell Reports · doi:10.1016/j.stemcr.2024.07.008 · Cited by 31
Advances in Central Nervous System Organoids: A Focus on Organoid-Based Models for Motor Neuron Disease
de Sá, R. V., Luna, M. C., & Pasterkamp, R. J. (2021) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2020.0337 · Cited by 31
Modeling Neurological Disorders in 3D Organoids Using Human-Derived Pluripotent Stem Cells
Bose, R., Banerjee, S., & Dunbar, G. L. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.640212 · Cited by 30
Emerging hiPSC Models for Drug Discovery in Neurodegenerative Diseases
Trudler, D., Ghatak, S., & Lipton, S. A. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22158196 · Cited by 30
‘Consciousnessoids’: clues and insights from human cerebral organoids for the study of consciousness
Lavazza, A. (2021) · Neuroscience of Consciousness · doi:10.1093/nc/niab029 · Cited by 30
Sustained type I interferon signaling after human immunodeficiency virus type 1 infection of human iPSC derived microglia and cerebral organoids
Boreland, A. J., Stillitano, A. C., Lin, H., Abbo, Y., Hart, R. P., Jiang, P., Pang, Z. P., & Rabson, A. B. (2024) · iScience · doi:10.1016/j.isci.2024.109628 · Cited by 30
PsychENCODE and beyond: transcriptomics and epigenomics of brain development and organoids
Jourdon, A., Scuderi, S., Capauto, D., Abyzov, A. S., & Vaccarino, F. M. (2020) · Neuropsychopharmacology · doi:10.1038/s41386-020-0763-3 · Cited by 29
The Impact of the Cellular Environment and Aging on Modeling Alzheimer's Disease in 3D Cell Culture Models
Hebisch, M., Klostermeier, S., Wolf, K., Boccaccini, A. R., Wolf, S. E., Tanzi, R. E., & Kim, D. Y. (2023) · Advanced Science · doi:10.1002/advs.202205037 · Cited by 31
Functional neurogenomics in autism spectrum disorders: A decade of progress
Bicks, L. K., & Geschwind, D. H. (2024) · Current Opinion in Neurobiology · doi:10.1016/j.conb.2024.102858 · Cited by 30
Self-citation
Functional Neural Networks in Human Brain Organoids
Gu, L., Cai, H., Chen, L., Gu, M., Tchieu, J. H., & Guo, F. (2024) · BME Frontiers · doi:10.34133/bmef.0065 · Cited by 29
Phenotypic complexities of rare heterozygous neurexin-1 deletions
Fernando, M. B., Fan, Y., Zhang, Y., Tokolyi, A., Murphy, A., Kammourh, S., Deans, P. J. M., Ghorbani, S., Onatzevitch, R., Pero, A., et al. (2025) · Nature · doi:10.1038/s41586-025-08864-9 · Cited by 30
The microbiota‐gut‐brain axis and epilepsy from a multidisciplinary perspective: Clinical evidence and technological solutions for improvement of in vitro preclinical models
Fusco, F., Perottoni, S., Giordano, C., Riva, A., Iannone, L. F., Caro, C. D., Russo, E., Albani, D., & Striano, P. (2022) · Bioengineering & Translational Medicine · doi:10.1002/btm2.10296 · Cited by 27
Recent Advances in Brain Organoid Technology for Human Brain Research
Jeong, E., Choi, S., & Cho, S. (2022) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.2c17467 · Cited by 29
Joint epigenome profiling reveals cell-type-specific gene regulatory programmes in human cortical organoids
Noack, F., Vangelisti, S., Ditzer, N., Chong, F., Albert, M., & Bonev, B. (2023) · Nature Cell Biology · doi:10.1038/s41556-023-01296-5 · Cited by 27
Modeling brain development and diseases with human cerebral organoids
Shi, Y., Wu, Q., & Wang, X. (2020) · Current Opinion in Neurobiology · doi:10.1016/j.conb.2020.09.006 · Cited by 28
Unraveling Human Brain Development and Evolution Using Organoid Models
Fernandes, S., Klein, D., & Marchetto, M. C. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.737429 · Cited by 27
Brain organoids: the quest to decipher human-specific features of brain development
Uzquiano, A., & Arlotta, P. (2022) · Current Opinion in Genetics & Development · doi:10.1016/j.gde.2022.101955 · Cited by 28
Cerebral Organoids as an Experimental Platform for Human Neurogenomics
Nowakowski, T. J., & Salama, S. R. (2022) · Cells · doi:10.3390/cells11182803 · Cited by 27
Electrophysiological and morphological characterization of single neurons in intact human brain organoids
Landry, C. R., Yip, M. C., Zhou, Y., Niu, W., Wang, Y., Yang, B., Wen, Z., & Forest, C. R. (2023) · Journal of Neuroscience Methods · doi:10.1016/j.jneumeth.2023.109898 · Cited by 26
Taming human brain organoids one cell at a time
Atamian, A., Cordón-Barris, L., & Quadrato, G. (2020) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2020.05.022 · Cited by 26
Three dimensional bioelectronic interfaces to small-scale biological systems
Park, Y., Chung, T. S., & Rogers, J. A. (2021) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2021.07.023 · Cited by 27
Modelling the central nervous system: tissue engineering of the cellular microenvironment
Walczak, P. A., Esteban, P. P., Bassett, D. C., & Hill, E. J. (2021) · Emerging Topics in Life Sciences · doi:10.1042/etls20210245 · Cited by 26
Defective proteostasis in induced pluripotent stem cell models of frontotemporal lobar degeneration
Mahali, S., Martinez, R., King, M., Verbeck, A., Harari, O., Benitez, B. A., Horie, K., Sato, C., Temple, S., & Karch, C. M. (2022) · Translational Psychiatry · doi:10.1038/s41398-022-02274-5 · Cited by 33
Modeling Autism Spectrum Disorders with Induced Pluripotent Stem Cell-Derived Brain Organoids
Santos, J. L. D. S., Araújo, C. D. A., Rocha, C. A. G., Costa‐Ferro, Z. S. M., & Souza, B. S. D. F. (2023) · Biomolecules · doi:10.3390/biom13020260 · Cited by 29
Retinal and Brain Organoids: Bridging the Gap Between in vivo Physiology and in vitro Micro-Physiology for the Study of Alzheimer’s Diseases
Brighi, C., Cordella, F., Chiriatti, L., Soloperto, A., & Angelantonio, S. D. (2020) · Frontiers in Neuroscience · doi:10.3389/fnins.2020.00655 · Cited by 25
Label-Free Quantification of Cell Cycle Synchronicity of Human Neural Progenitor Cells Based on Electrophysiology Phenotypes
Honrado, C., Michel, N., Moore, J. H., Salahi, A., Porterfield, V. M., McConnell, M. J., & Swami, N. S. (2020) · ACS Sensors · doi:10.1021/acssensors.0c02022 · Cited by 26
Brain Organoids: Studying Human Brain Development and Diseases in a Dish
Xu, J., & Wen, Z. (2021) · Stem Cells International · doi:10.1155/2021/5902824 · Cited by 25
Modeling Developmental Brain Diseases Using Human Pluripotent Stem Cells-Derived Brain Organoids – Progress and Perspective
Bhattacharya, A., Choi, W. W. Y., Muffat, J., & Li, Y. (2021) · Journal of Molecular Biology · doi:10.1016/j.jmb.2021.167386 · Cited by 24
Phospho-seq: integrated, multi-modal profiling of intracellular protein dynamics in single cells
Blair, J. D., Hartman, A., Zenk, F. L., Wahle, P., Brancati, G., Dalgarno, C., Treutlein, B., & Satija, R. (2025) · Nature Communications · doi:10.1038/s41467-025-56590-7 · Cited by 27
Mass spectrometry imaging as an emerging tool for studying metabolism in human brain organoids
Cappuccio, G., Khalil, S. M., Osenberg, S., Li, F., & Maletić‐Savatić, M. (2023) · Frontiers in Molecular Biosciences · doi:10.3389/fmolb.2023.1181965 · Cited by 24
Patterning ganglionic eminences in developing human brain organoids using a morphogen-gradient-inducing device
Pavon, N., Diep, K., Yang, F., Sebastian, R., Martinez-Martin, B., Ranjan, R., Sun, Y., & Pak, C. (2024) · Cell Reports Methods · doi:10.1016/j.crmeth.2023.100689 · Cited by 24
Copy number variants (CNVs): a powerful tool for iPSC-based modelling of ASD
Drakulić, D., Djurovic, S., Syed, Y. A., Trattaro, S., Caporale, N., Falk, A., Ofir, R., Heine, V. M., Chawner, S. J. R. A., Moreno, A. R., et al. (2020) · Molecular Autism · doi:10.1186/s13229-020-00343-4 · Cited by 24
Modeling the precise interaction of glioblastoma with human brain region-specific organoids
Fan, Q., Wang, H., Gu, T., Liu, H., Deng, P., Li, B., Yang, H., Mao, Y. Q., & Shao, Z. (2024) · iScience · doi:10.1016/j.isci.2024.109111 · Cited by 24
Dysregulation of miRNA expression and excitation in MEF2C autism patient hiPSC-neurons and cerebral organoids
Trudler, D., Ghatak, S., Bula, M., Parker, J., Talantova, M. V., Luevanos, M., Labra, S. R., Grabauskas, T., Noveral, S. M., Teranaka, M., et al. (2024) · Molecular Psychiatry · doi:10.1038/s41380-024-02761-9 · Cited by 24
Self-citation
Central nervous system vascularization in human embryos and neural organoids
Boutom, S., Silva, T. P., Palecek, S. P., Shusta, E. V., Fernandes, T. G., & Ashton, R. S. (2024) · Cell Reports · doi:10.1016/j.celrep.2024.115068 · Cited by 26

Authors: Yoon, S.-J., Elahi, L. S., Pașca, A. M., Marton, R. M., Gordon, A., Revah, O., Miura, Y., Walczak, E. M., Holdgate, G. M., Fan, H. C., Huguenard, J. R., Geschwind, D. H., & Pașca, S. P.
Affiliations: Stanford Medicine (US)
University of California, Los Angeles (US)
BD Biosciences (United States) (US)
Corresponding author: Pașca, Sergiu P.
ORCID: Yoon, Se-Jin
Elahi, Lubayna S.
Pașca, Anca M.
Marton, Rebecca M.
Gordon, Aaron
Revah, Omer
Miura, Yuki
Fan, H. Christina
Huguenard, John R.
Geschwind, Daniel H.
Pașca, Sergiu P.
Venue: Nature Methods
Publisher: Springer Science and Business Media LLC
Published: 2018-12-20
Volume: 16
Issue: 1
Pages: 75-78
ISSN: 1548-7091 (ISSN-L)
1548-7105
DOI: 10.1038/s41592-018-0255-0
PMC: PMC6677388
OpenAlex: W2904130550
Cited by: 507
References: 28
Citations by year: 2026: 66 · 2025: 74 · 2024: 57 · 2023: 59 · 2022: 67 · 2021: 70 · 2020: 76 · 2019: 35
FWCI: 16.09
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: www.springer.com/tdm (tdm)
www.springer.com/tdm (vor)
Locations: Nature Methods (publishedVersion)
PubMed (publishedVersion)
Europe PMC (PubMed Central) (acceptedVersion)
PubMed Central (acceptedVersion)
eScholarship (California Digital Library) (submittedVersion)
Funders: New York Stem Cell Foundation
Nature
National Institutes of Health
National Institute of Mental Health (R01MH107800, U01MH115745, U01MH115746)
National Institute of Neurological Disorders and Stroke (R01NS034774)
Eunice Kennedy Shriver National Institute of Child Health and Human Development (K12HD000850)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Keywords: cortical organoids, human pluripotent stem cells, brain development, xeno-free culture, feeder-free culture, disease modeling
MeSH terms: Cell Line, Humans, Organoids, Reproducibility of Results, Prosencephalon, Sequence Analysis, RNA, Tissue Engineering, Pluripotent Stem Cells, Single-Cell Analysis

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Reliability of human cortical organoid generation

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Self-check

Citations

Cites

Cited by

Related

Metadata

Record

About these checks