Cited by 507Pluripotent Stem Cells ResearchNeurogenesis and neuroplasticity mechanismsDevelopmental Biology and Gene Regulation

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Reliability of human cortical organoid generation

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Yoon, Se-Jin, Elahi, Lubayna S., Pașca, Anca M., Marton, Rebecca M., Gordon, Aaron, Revah, Omer, Miura, Yuki, Walczak, Elisabeth M., Holdgate, Gwendolyn M., Fan, H. Christina, Huguenard, John R., Geschwind, Daniel H., Pașca, Sergiu P.·2019·Nature Methods

DOI 10.1038/s41592-018-0255-0PMC PMC6677388

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Abstract

The differentiation of pluripotent stem cells in three-dimensional cultures can recapitulate key aspects of brain development, but protocols are prone to variable results. Here we differentiated multiple human pluripotent stem cell lines for over 100 d using our previously developed approach to generate brain-region-specific organoids called cortical spheroids and, using several assays, found that spheroid generation was highly reliable and consistent. We anticipate the use of this approach for large-scale differentiation experiments and disease modeling.

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Authors: Yoon, S.-J., Elahi, L. S., Pașca, A. M., Marton, R. M., Gordon, A., Revah, O., Miura, Y., Walczak, E. M., Holdgate, G. M., Fan, H. C., Huguenard, J. R., Geschwind, D. H., & Pașca, S. P.
Affiliations: Stanford Medicine (US)
University of California, Los Angeles (US)
BD Biosciences (United States) (US)
Corresponding author: Pașca, Sergiu P.
ORCID: Yoon, Se-Jin
Elahi, Lubayna S.
Pașca, Anca M.
Marton, Rebecca M.
Gordon, Aaron
Revah, Omer
Miura, Yuki
Fan, H. Christina
Huguenard, John R.
Geschwind, Daniel H.
Pașca, Sergiu P.
Venue: Nature Methods
Publisher: Springer Science and Business Media LLC
Published: 2018-12-20
Volume: 16
Issue: 1
Pages: 75-78
ISSN: 1548-7091 (ISSN-L)
1548-7105
DOI: 10.1038/s41592-018-0255-0
PMC: PMC6677388
OpenAlex: W2904130550
Cited by: 507
References: 28
Citations by year: 2026: 66 · 2025: 74 · 2024: 57 · 2023: 59 · 2022: 67 · 2021: 70 · 2020: 76 · 2019: 35
FWCI: 16.09
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: www.springer.com/tdm (tdm)
www.springer.com/tdm (vor)
Locations: Nature Methods (publishedVersion)
PubMed (publishedVersion)
Europe PMC (PubMed Central) (acceptedVersion)
PubMed Central (acceptedVersion)
eScholarship (California Digital Library) (submittedVersion)
Funders: New York Stem Cell Foundation
Nature
National Institutes of Health
National Institute of Mental Health (R01MH107800, U01MH115745, U01MH115746)
National Institute of Neurological Disorders and Stroke (R01NS034774)
Eunice Kennedy Shriver National Institute of Child Health and Human Development (K12HD000850)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Keywords: cortical organoids, human pluripotent stem cells, brain development, xeno-free culture, feeder-free culture, disease modeling
MeSH terms: Cell Line, Humans, Organoids, Reproducibility of Results, Prosencephalon, Sequence Analysis, RNA, Tissue Engineering, Pluripotent Stem Cells, Single-Cell Analysis

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