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Buprenorphine and methadone differentially alter early brain development in human cortical organoids

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From Organoids to Assembloids: Experimental Approaches to Study Human Neuropsychiatric Disorders

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Pluripotent stem-cell-based screening uncovers sildenafil as a mitochondrial disease therapy

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Methadone Suppresses Neuronal Function and Maturation in Human Cortical Organoids

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WWOX-Related Neurodevelopmental Disorders: Models and Future Perspectives

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Single-nucleus ribonucleic acid-sequencing and spatial transcriptomics reveal the cardioprotection of Shexiang Baoxin Pill (SBP) in mice with myocardial ischemia-reperfusion injury

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Advanced Cellular Models for Rare Disease Study: Exploring Neural, Muscle and Skeletal Organoids

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Engineering Brain Organoids: Toward Mature Neural Circuitry with an Intact Cytoarchitecture

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Assembloid model to study loop circuits of the human nervous systemPreprint

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Neural Organoids, a Versatile Model for Neuroscience

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Three-dimensional microfluidics with dynamic fluidic perturbation promotes viability and uniformity of human cerebral organoids

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Complex in vitro models positioned for impact to drug testing in pharma: a review

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Studying Heterotypic Cell–Cell Interactions in the Human Brain Using Pluripotent Stem Cell Models for Neurodegeneration

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Parechovirus infection in human brain organoids: host innate inflammatory response and not neuro-infectivity correlates to neurologic disease

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Modeling cell-cell interactions in the brain using cerebral organoids

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What Makes Organoids Good Models of Human Neurogenesis?

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Advances in 3D neural, vascular and neurovascular models for drug testing and regenerative medicine

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Human pluripotent stem cell-derived brain organoids as in vitro models for studying neural disorders and cancer

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Methodologies for Generating Brain Organoids to Model Viral Pathogenesis in the CNS

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Brain organoid models for studying the function of iPSC-derived microglia in neurodegeneration and brain tumours

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Generation of Neural Organoids and Their Application in Disease Modeling and Regenerative Medicine

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Neuronal hyperexcitability and ion channel dysfunction in CDKL5-deficiency patient iPSC-derived cortical organoids

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Integrated Manufacturing Platform for Cortical Organoids Reveals Early Phosphatase and Prefoldin Dysregulation in MAPT V337M Neurons

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Effects of the Selective Serotonin Reuptake Inhibitor Fluoxetine on Developing Neural Circuits in a Model of the Human Fetal Cortex

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Progress of Induced Pluripotent Stem Cell Technologies to Understand Genetic Epilepsy

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Proof of concept for brain organoid-on-a-chip to create multiple domains in forebrain organoids

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Cortical Organoids to Model Microcephaly

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Use of standard U-bottom and V-bottom well plates to generate neuroepithelial embryoid bodies

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Human organoid tumor transplantation identifies functional glioblastoma-microenvironment communication mediated by PTPRZ1

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iPSC toolbox for understanding and repairing disrupted brain circuits in autism

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Efficient Gene Expression in Human Stem Cell Derived-Cortical Organoids Using Adeno Associated Virus

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Host circuit engagement of human cortical organoids transplanted in rodents

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Biphasic Electrical Pulse by a Micropillar Electrode Array Enhances Maturation and Drug Response of Reprogrammed Cardiac Spheroids

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Oxygen-induced stress reveals context-specific gene regulatory effects in human brain organoidsPreprint

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Probing the molecular and cellular pathological mechanisms of schizophrenia using human induced pluripotent stem cell models

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The exon junction complex component EIF4A3 is essential for mouse and human cortical progenitor mitosis and neurogenesis

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Chronic Opioid Treatment Arrests Neurodevelopment and Alters Synaptic Activity in Human Midbrain Organoids

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Utilising Human Myometrial and Uterine Fibroid Stem Cell‐Derived Three Dimentional Organoids as a Robust Model System for Understanding the Pathophysiology of Uterine Fibroids

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An insight into the iPSCs-derived two-dimensional culture and three-dimensional organoid models for neurodegenerative disorders

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The RNA-binding protein EIF4A3 promotes axon development by direct control of the cytoskeleton

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Flexible and stretchable bioelectronics for organoids

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Focus on organoids: cooperation and interconnection with extracellular vesicles – Is this the future of in vitro modeling?

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Human PSCs determine the competency of cerebral organoid differentiation via FGF signaling and epigenetic mechanisms

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Advancing Glioblastoma Research with Innovative Brain Organoid-Based Models

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Issues with Cancer Spheroid Models in Therapeutic Drug Screening

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Harnessing pluripotent stem cells as models to decipher human evolution

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Integrating Engineering, Automation, and Intelligence to Catalyze the Biomedical Translation of Organoids

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The Brain Protein Atlas: A conglomerate of proteomics datasets of human neural tissue

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Hydrogel-integrated Microfluidic Systems for Advanced Stem Cell Engineering

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A phenotypic brain organoid atlas and biobank for neurodevelopmental disorders

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Aberrant neurodevelopment in human iPS cell‐derived models of Alexander disease

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Quantitative profiling of human brain organoid cell diversity across four protocols and multiple cell lines

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Transcriptomic Mapping of Neural Diversity, Differentiation and Functional Trajectory in iPSC-Derived 3D Brain Organoid Models

Kiaee, K., Jodat, Y. A., Bassous, N. J., Matharu, N. K., & Shin, S. R. (2021) · Cells · doi:10.3390/cells10123422 · Cited by 13

Longitudinal Transcriptomic Analysis of Human Cortical Spheroids Identifies Axonal Dysregulation in the Prenatal Brain as a Mediator of Genetic Risk for Schizophrenia

Akkouh, I. A., Ueland, T., Szabó, A., Hughes, T., Smeland, O. B., Andreassen, O. A., Osete, J. R., & Djurovic, S. (2023) · Biological Psychiatry · doi:10.1016/j.biopsych.2023.08.017 · Cited by 13

Genetically Encoded Tools for Research of Cell Signaling and Metabolism under Brain Hypoxia

Kostyuk, A. I., Kokova, A. D., Podgorny, O. V., Kelmanson, I. V., Fetisova, E. S., Belousov, V. V., & Bilan, D. S. (2020) · Antioxidants · doi:10.3390/antiox9060516 · Cited by 13

Human Excitatory Cortical Neurospheroids Coupled to High-Density MEAs: A Valid Platform for Functional Tests

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Evaluation of Neurotoxicity With Human Pluripotent Stem Cell–Derived Cerebral Organoids

Parmentier, T., LaMarre, J., & Lalonde, J. (2023) · Current Protocols · doi:10.1002/cpz1.744 · Cited by 12

Engineering the Physical Microenvironment into Neural Organoids for Neurogenesis and Neurodevelopment

Li, M., Sun, H., Hou, Z., Hao, S., Jin, L., & Wang, B. (2023) · Small · doi:10.1002/smll.202306451 · Cited by 12

The application of brain organoid for drug discovery in mitochondrial diseases

Liang, K. X. (2024) · The International Journal of Biochemistry & Cell Biology · doi:10.1016/j.biocel.2024.106556 · Cited by 12

Human brain organoids for understanding substance use disorders

Li, K., Gu, L., Cai, H., Lu, H., Mackie, K., & Guo, F. (2024) · Drug Metabolism and Pharmacokinetics · doi:10.1016/j.dmpk.2024.101036 · Cited by 14

The generation and properties of human cortical organoids as a disease model for malformations of cortical development

Zhang, X.-P., Wang, X., Wang, S.-N., & Miao, C. (2023) · Neural Regeneration Research · doi:10.4103/1673-5374.369100 · Cited by 13

Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation

Sambo, D. O., Li, J., Brickler, T., & Chetty, S. (2019) · Journal of Visualized Experiments · doi:10.3791/59833 · Cited by 13

Enhancer-targeted CRISPR-A rescues haploinsufficiency and mutant phenotypes in organoid models of autismPreprint

Chen, G. T., Nair, G., Osorio, A. J., Holley, S. M., Ghassemzadeh, K., Zhou, Y., Gonzalez, J., Martin, J. M., Lu, C., Sanjana, N. E., et al. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.03.13.584921 · Cited by 12

High glucose concentrations mask cellular phenotypes in a stem cell model of tuberous sclerosis complex

Rocktäschel, P., Sen, A., & Cader, M. Z. (2019) · Epilepsy & Behavior · doi:10.1016/j.yebeh.2019.106581 · Cited by 12

Cerebral organoids: emerging ex vivo humanoid models of glioblastoma

Papaioannou, M.-D., Sangster, K. T., Sajid, R. S., Djuric, U., & Diamandis, P. (2020) · Acta Neuropathologica Communications · doi:10.1186/s40478-020-01077-3 · Cited by 11

Review of studies of severe acute respiratory syndrome related coronavirus–2 pathogenesis in human organoid models

Egilmezer, E., & Rawlinson, W. D. (2021) · Reviews in Medical Virology · doi:10.1002/rmv.2227 · Cited by 11

Defense of COVID-19 by Human Organoids

Lv, T., Meng, F., Yu, M., Huang, H., Lin, X., & Zhao, B. T. (2021) · Phenomics · doi:10.1007/s43657-021-00015-0 · Cited by 11

Human cerebral spheroids undergo 4-aminopyridine-induced, activity associated changes in cellular composition and microrna expression

Parmentier, T., James, F., Hewitson, E., Bailey, C. D. C., Werry, N., Sheridan, S., Perlis, R., Perreault, M., Gaitero, L., Lalonde, J., et al. (2022) · Scientific Reports · doi:10.1038/s41598-022-13071-x · Cited by 9

Rare and undiagnosed diseases: From disease-causing gene identification to mechanism elucidation

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Pharmacological Manipulation of Wnt/β-Catenin Signaling Pathway in Human Neural Precursor Cells Alters Their Differentiation Potential and Neuronal Yield

Telias, M., & Ben‐Yosef, D. (2021) · Frontiers in Molecular Neuroscience · doi:10.3389/fnmol.2021.680018 · Cited by 11

In Vitro Recapitulation of Neuropsychiatric Disorders with Pluripotent Stem Cells-Derived Brain Organoids

Gulimiheranmu, M., Li, S., & Zhou, J. (2021) · International Journal of Environmental Research and Public Health · doi:10.3390/ijerph182312431 · Cited by 11

Pluripotent stem cell‐derived organoids: A brief history of curiosity‐led discoveries

Lancaster, M. A. (2024) · BioEssays · doi:10.1002/bies.202400105 · Cited by 11

Manufacturing Uniform Cerebral Organoids for Neurological Disease Modeling and Drug Evaluation

Hong, H., Jun, Y., Yoon, S.-B., Park, S., Lee, J., Jang, J., Nam, H. J., & Cho, H. (2024) · Biomaterials Research · doi:10.34133/bmr.0104 · Cited by 9

Protocol for generating and using human iPSC-derived microglia-containing air-liquid-interface cortical organoid cultures

Luna, M. C., Mars, M., Jakobs, C. E., Huffels, C. F. M., Ermakov, A., Hol, E. M., & Pasterkamp, R. J. (2025) · STAR Protocols · doi:10.1016/j.xpro.2025.103915 · Cited by 10

Prenatal Drugs and Their Effects on the Developing Brain: Insights From Three-Dimensional Human Organoids

Stankovic, I. N., & Colak, D. (2022) · Frontiers in Neuroscience · doi:10.3389/fnins.2022.848648 · Cited by 10

Advances in Recapitulating Alzheimer’s Disease Phenotypes Using Human Induced Pluripotent Stem Cell-Based In Vitro Models

Hasan, M. F., & Trushina, E. V. (2022) · Brain Sciences · doi:10.3390/brainsci12050552 · Cited by 10

Using iPSC Models to Understand the Role of Estrogen in Neuron–Glia Interactions in Schizophrenia and Bipolar Disorder

de Assis, D. R., Szabó, A., Osete, J. R., Puppo, F., O’Connell, K. S., Akkouh, I. A., Hughes, T., Frei, E., Andreassen, O. A., & Djurovic, S. (2021) · Cells · doi:10.3390/cells10020209 · Cited by 10

Production of Phenotypically Uniform Human Cerebral Organoids from Pluripotent Stem Cells

Sivitilli, A., Ghiasi, P., & Attisano, L. (2021) · BIO-PROTOCOL · doi:10.21769/bioprotoc.3985 · Cited by 10

Neuron and astrocyte aggregation and sorting in three-dimensional neuronal constructs

Hasan, M. F., & Berdichevsky, Y. (2021) · Communications Biology · doi:10.1038/s42003-021-02104-2 · Cited by 10

Unraveling pathological mechanisms in neurological disorders: the impact of cell-based and organoid models

Langlie, J. A., Mittal, R., Finberg, A., Bencie, N. B., Mittal, J., Omidian, H., Omidi, Y., & Eshraghi, A. A. (2022) · Neural Regeneration Research · doi:10.4103/1673-5374.335836 · Cited by 10

Optimization and scaling of patient-derived brain organoids uncovers deep phenotypes of diseasePreprint

Shah, K. D., Bedi, R., Rogozhnikov, A., Ramkumar, P., Tong, Z., Rash, B. G., Stanton, M. M., Sorokin, J. M., Apaydin, C., Batarse, A., et al. (2020) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2020.08.26.251611 · Cited by 11

Understanding monocyte-driven neuroinflammation in Alzheimer’s disease using human cortical organoid microphysiological systems

Tian, C., Ao, Z., Cerneckis, J., Cai, H., Chen, L., Niu, H., Takayama, K., Kim, J., Shi, Y., Gu, M., et al. (2025) · Science Advances · doi:10.1126/sciadv.adu2708 · Cited by 9

Emerging brain organoids: 3D models to decipher, identify and revolutionize brain

Zhao, Y., Wang, T., Liu, J., Wang, Z., & Lu, Y. (2025) · Bioactive Materials · doi:10.1016/j.bioactmat.2025.01.025 · Cited by 9

Elastic Thermoplastic Polyurethane/Graphene Microneedle-Mesh Interfaces via Microfluidic Patterning for Electrophysiology in Neural Organoids

Wu, Y. Q., Mei, R., Zhou, Y., Qi, J., Chen, H., & Jiang, X. (2025) · ACS Nano · doi:10.1021/acsnano.5c08827 · Cited by 9

Reliability of human retina organoid generation from hiPSC-derived neuroepithelial cysts

Carido, M., Völkner, M., Steinheuer, L. M., Wagner, F., Kurth, T., Dumler, N., Ulusoy, S., Wieneke, S., Norniella, A. V., Golfieri, C., et al. (2023) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2023.1166641 · Cited by 9

Building brains: using brain organoids to study neural development and disease

Lyon, L. (2019) · Brain · doi:10.1093/brain/awz308 · Cited by 9

A multi-looping chromatin signature predicts dysregulated gene expression in neurons with familial Alzheimer’s disease mutationsPreprint

Chandrashekar, H., Simándi, Z., Choi, H., Ryu, H.-S., Waldman, A. J., Nikish, A., Muppidi, S. S., Gong, W., Paquet, D., & Phillips‐Cremins, J. E. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.02.27.582395 · Cited by 10

AAVS1 ‐targeted, stable expression of ChR2 in human brain organoids for consistent optogenetic control

Hong, S.-J., Lee, J. H., Kim, Y., Kim, E. H., & Shin, K. (2024) · Bioengineering & Translational Medicine · doi:10.1002/btm2.10690 · Cited by 8

Single-cell technology for cell-based drug delivery and pharmaceutical research

Hu, H., Fan, Y., Wang, J., Zhang, J., Lyu, Y., Hou, X., Cui, J., Zhang, Y., Gao, J., Zhang, T., et al. (2025) · Journal of Controlled Release · doi:10.1016/j.jconrel.2025.113587 · Cited by 8

CRISPR screens in human neural organoids and assembloids

Meng, X., Reis, N., Bassik, M. C., & Pașca, S. P. (2025) · Nature Protocols · doi:10.1038/s41596-025-01299-6 · Cited by 8

Human cortical organoids recapitulate inter-individual variability in infant brain-growth trajectories

Glass, M. R., Matoba, N., Beltran, A. A., Patel, N. K., Farah, T. M., Eswar, K. K., Bhargava, S., Huang, K., Curtin, I., Ahmed, S., et al. (2025) · Cell stem cell · doi:10.1016/j.stem.2025.12.001 · Cited by 11

Autophagy Activators Normalize Aberrant Tau Proteostasis and Rescue Synapses in Human Familial Alzheimer's Disease iPSC‐Derived Cortical Organoids

Labra, S. R., Compher, J., Prabhavalkar, A., Almaraz, M., Kwong, C. C., Baal, C., Talantova, M. V., Dolatabadi, N., Piña‐Sanz, J., Wang, Y., et al. (2026) · Advanced Science · doi:10.1002/advs.202514783 · Cited by 9

Temporal controls over cortical projection neuron fate diversity

Klingler, E. (2023) · Current Opinion in Neurobiology · doi:10.1016/j.conb.2023.102677 · Cited by 10

Robust Tissue Fabrication for Long-Term Culture of iPSC-Derived Brain Organoids for Aging Research

Koch, L. S., Buentello, D. C., & Broersen, K. (2023) · Journal of Visualized Experiments · doi:10.3791/64586 · Cited by 8

A narrative review of organoids for investigating organ aging: opportunities and challenges

Sun, X., Sun, F., Zhang, Y., Qu, J. T., Zhang, W., & Liu, G. (2023) · Journal of Bio-X Research · doi:10.1097/jbr.0000000000000139 · Cited by 8

Stem Cell Therapy in Neonatal Hypoxic-Ischemic Encephalopathy and Cerebral Palsy: a Bibliometric Analysis and New Strategy

Xiao, Q., Geng, M., Sun, Y., Pi, Y., & Xiong, L. (2023) · Molecular Neurobiology · doi:10.1007/s12035-023-03848-0 · Cited by 8

Highly reproducible human brain organoids recapitulate cerebral cortex cellular diversity.Preprint

Velasco, S., Paulsen, B. D. S., & Arlotta, P. (2019) · doi:10.21203/rs.2.9542/v1 · Cited by 16

A simplified protocol for the generation of cortical brain organoids

Eigenhuis, K. N., Somsen, H. B., van der Kroeg, M., Smeenk, H., Korporaal, A. L., Kushner, S. A., de Vrij, F. M. S., & van den Berg, D. L. C. (2023) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2023.1114420 · Cited by 19

Generation of cerebral organoids from human pluripotent stem cells

Lancaster, M. A., & Knoblich, J. A. (2014) · Nature Protocols · doi:10.1038/nprot.2014.158 · Cited by 1,823

Generating Cerebral Organoids from Human Pluripotent Stem Cells

Chew, L. H., Añonuevo, A., & Knock, E. (2021) · Methods in molecular biology · doi:10.1007/978-1-0716-1783-0_15 · Cited by 11

A Robust Methodological Framework for Generating Whole‐Brain and Cortical Organoids From Diverse Healthy‐ and Patient‐Derived Induced Pluripotent Stem Cell Lines

Fournier, T., Pugliese, E., Montagne, B., Cimmaruta, C., & Ricchetti, M. (2026) · Biology of the Cell · doi:10.1111/boc.70051