Cited by 1,026Neurotransmitter Receptor Influence on BehaviorStress Responses and CortisolTreatment of Major Depression

Sucrose preference test for measurement of stress-induced anhedonia in mice

Liu, Meng-Ying, Yin, Chun-Yu, Zhu, Li-Juan, Zhu, Xian-Hui, Xu, Chu, Luo, Chun-Xia, Chen, Hongshan, Zhu, Dong-Ya +1 more·2018·Nature Protocols

Abstract

Anhedonia is the inability to experience pleasure from rewarding or enjoyable activities and is a core symptom of depression in humans. Here, we describe a protocol for the measurement of anhedonia in mice, in which anhedonia is measured by a sucrose preference test (SPT) based on a two-bottle choice paradigm. A reduction in the sucrose preference ratio in experimental relative to control mice is indicative of anhedonia. To date, inconsistent and variable results have been reported following the use of the SPT by different groups, probably due to the use of different protocols and equipment. In this protocol, we describe how to set up a clearly defined apparatus for SPT and provide a detailed protocol to ensure greater consistency when carrying out SPT. This optimized protocol is highly sensitive, reliable, and adaptable for evaluation of chronic stress-related anhedonia, as well as morphine-induced dependence. The whole SPT, including adaptation, baseline measurement, and testing, takes 8 d.

Integrity signals

Registry

Crossref · 10.1038/s41596-018-0011-z

Cites

43 of 43 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Liu, M.-Y., Yin, C.-Y., Zhu, L.-J., Zhu, X.-H., Xu, C., Luo, C.-X., Chen, H., Zhu, D.-Y., & Zhou, Q.-G.
Affiliations
  • Nanjing Drum Tower Hospital (CN)
  • Southeast University (CN)
  • Nanjing Medical University (CN)
  • Second Affiliated Hospital of Nanjing Medical University (CN)
Corresponding author
  • Chen, Hongshan
  • Zhu, Dong-Ya
  • Zhou, Qi-Gang
Venue
Nature Protocols
Published
2018-07
Volume
13
Issue
7
Pages
1686-1698
ISSN
  • 1750-2799 (ISSN-L)
  • 1754-2189
OpenAlex
W2865698779
Cited by
1,026
References
43
Citations by year
2026: 135 · 2025: 181 · 2024: 177 · 2023: 168 · 2022: 153 · 2021: 125 · 2020: 53 · 2019: 29 · 2018: 4
FWCI
23.89
Type
journal article
Language
en
Open access
closed
Locations
  • Nature Protocols (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
  • Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
  • Health Sciences › Medicine › Pharmacology › Treatment of Major Depression
Keywords
anhedonia, sucrose preference test, chronic stress, depression, two-bottle choice, morphine dependence, reward, mice
MeSH terms
Animals, Diagnostic Tests, Routine, Disease Models, Animal, Food Preferences, Stress, Psychological, Sucrose, Sweetening Agents, Mice, Anhedonia

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review