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Cited by 55Neuroscience and Neuropharmacology ResearchAdvanced Proteomics Techniques and ApplicationsGenetics and Neurodevelopmental Disorders

Correlation profiling of brain sub-cellular proteomes reveals co-assembly of synaptic proteins and subcellular distribution

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Pandya, Nikhil J., Koopmans, Frank, Slotman, Johan A., Paliukhovich, Iryna, Houtsmuller, Adriaan B., Smit, August B., Li, Ka Wan·2017·Scientific Reports
DOI 10.1038/s41598-017-11690-3PMC PMC5608747
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Abstract

Protein correlation profiling might assist in defining co-assembled proteins and subcellular distribution. Here, we quantified the proteomes of five biochemically isolated mouse brain cellular sub-fractions, with emphasis on synaptic compartments, from three brain regions, hippocampus, cortex and cerebellum. We demonstrated the expected co-fractionation of canonical synaptic proteins belonging to the same functional groups. The enrichment profiles also suggested the presence of many novel pre- and post-synaptic proteins. Using super-resolution microscopy on primary neuronal culture we confirmed the postsynaptic localization of PLEKHA5 and ADGRA1. We further detected profound brain region specific differences in the extent of enrichment for some functionally associated proteins. This is exemplified by different AMPA receptor subunits and substantial differences in sub-fraction distribution of their potential interactors, which implicated the differences of AMPA receptor complex compositions. This resource aids the identification of proteins partners and subcellular distribution of synaptic proteins.

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  • GluA3 subunits are the most enriched within the PSD under basal conditions.

    “A recent proteomics study has even found that GluA3 subunits are the most enriched within the PSD under basal conditions (Pandya et al., 2017).”

    From The AMPA Receptor Code of Synaptic Plasticity (2018) · cites this paper as Pandya et al., 2017

    In Results and Discussion, “Glutamate receptors and their interacting proteins” (page unavailable), GRIA3/GluA3 shows strong PSD association, but GRIA4 has the same reported 0.99 correlation. The paper therefore supports GluA3 as highly PSD-enriched, not as uniquely the most enriched subunit; it also does not explicitly characterize the comparison as being under basal conditions. (LLM, conf=0.86)

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Authors
Pandya, N. J., Koopmans, F., Slotman, J. A., Paliukhovich, I., Houtsmuller, A. B., Smit, A. B., & Li, K. W.
Affiliations
  • Amsterdam Neuroscience (NL)
  • Erasmus MC (NL)
Corresponding author
Li, Ka Wan
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2017-09-21
Volume
7
Issue
1
Pages
12107-12107
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/s41598-017-11690-3
PMC
PMC5608747
OpenAlex
W2756067497
Cited by
55
References
2
Citations by year
2026: 5 · 2025: 2 · 2024: 5 · 2023: 6 · 2022: 7 · 2021: 8 · 2020: 9 · 2019: 9 · 2018: 3 · 2017: 1
FWCI
2.95
Type
journal article
Language
en
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • EUR Research Repository (Erasmus University Rotterdam) (publishedVersion, other-oa)
  • VU Research Portal (publishedVersion, other-oa)
  • EUR Research Repository (Erasmus University Rotterdam) (submittedVersion, other-oa)
  • RePub (Erasmus University Rotterdam) (submittedVersion, other-oa)
  • Data Archiving and Networked Services (DANS) (submittedVersion, other-oa)
Funders
  • European Commission (242167)
  • Nederlandse Organisatie voor Wetenschappelijk Onderzoek (645-000-003)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetics and Neurodevelopmental Disorders
Keywords
synaptic proteins, subcellular proteome, postsynaptic localization, AMPA receptor subunits, regional brain differences, hippocampus, cerebral cortex, cerebellum, super-resolution microscopy, primary neurons
MeSH terms
Animals, Brain Chemistry, Cells, Cultured, Cerebellum, Cerebral Cortex, Hippocampus, Mice, Inbred C57BL, Neurons, Subcellular Fractions, Synapses, Proteome, Proteomics, Receptors, G-Protein-Coupled, Intracellular Signaling Peptides and Proteins, Mice, Tandem Mass Spectrometry

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