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Cited by 269Helicobacter pylori-related gastroenterology studiesGut microbiota and healthOral microbiology and periodontitis research

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Increased Abundance of Clostridium and Fusobacterium in Gastric Microbiota of Patients with Gastric Cancer in Taiwan

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Hsieh, Yung-Yu, Tung, Shui-Yi, Pan, Hung-Yu, Yen, Chih-Wei, Xu, Huang-Wei, Lin, Ying-Jhen, Deng, Yi-Fang, Hsu, Wan-Ting, Wu, Cheng-Shyong, Li, Chin·2018·Scientific Reports
DOI 10.1038/s41598-017-18596-0PMC PMC5760541
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Abstract

Helicobacter pylori is recognised as a main risk factor for gastric cancer. However, approximately half of the patients with gastritis are negative for H. pylori infection, and the abundance of H. pylori decreases in patients with cancer. In the current study, we profiled gastric epithelium-associated bacterial species in patients with gastritis, intestinal metaplasia, and gastric cancer to identify additional potential pathogenic bacteria. The overall composition of the microbiota was similar between the patients with gastritis and those with intestinal metaplasia. H. pylori was present in half of the non-cancer group, and the dominant bacterial species in the H. pylori-negative patients were Burkholderia, Enterobacter, and Leclercia. The abundance of those bacteria was similar between the cancer and non-cancer groups, whereas the frequency and abundance of H. pylori were significantly lower in the cancer group. Instead, Clostridium, Fusobacterium, and Lactobacillus species were frequently abundant in patients with gastric cancer, demonstrating a gastric cancer-specific bacterial signature. A receiver operating characteristic curve analysis showed that Clostridium colicanis and Fusobacterium nucleatum exhibited a diagnostic ability for gastric cancer. Our findings indicate that the gastric microenvironment is frequently colonised by Clostridium and Fusobacterium in patients with gastric cancer.

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Figure 1 Identification of changes in the composition of the microbiota between patients with gastric cancer and those with other gastrointestinal disorders by 16S ribosomal DNA metagenomic analysis. (A) The abundance of Helicobacter pylori in the patients’ microbiota. (B) The proportions of high-abundance in-transit microbes. (C) The abundance of Burkholderia, Enterobacter, and Leclercia. (D) The abundance of Clostridium, Fusobacterium, and Lactobacillus.
Figure 1 Identification of changes in the composition of the microbiota between patients with gastric cancer and those with other gastrointestinal disorders by 16S ribosomal DNA metagenomic analysis. (A) The abundance of Helicobacter pylori in the patients’ microbiota. (B) The proportions of high-abundance in-transit microbes. (C) The abundance of Burkholderia, Enterobacter, and Leclercia. (D) The abundance of Clostridium, Fusobacterium, and Lactobacillus.CC BY · PubMed Central
Figure 2 Bacteria specifically enriched in the gastric cancer-associated microbiota.
Figure 2 Bacteria specifically enriched in the gastric cancer-associated microbiota.CC BY · PubMed Central
Figure 3 Identification of the bacterial signature of gastric cancer. Multidimensional analyses were performed considering various combinations of the following bacterial species: Clostridium colicanis (Cc), Fusobacterium canifelinum (Fc), Fusobacterium nucleatum (Fn), Lactobacillus gasseri (Lg), Lactobacillus reuteri (Lr), Megasphaera micronuciformis (Mm), Prevotella intermedia (Pi), Prevotella oris (Po), Streptococcus gordonii (Sg), and Streptococcus parasanguinis (Sp).
Figure 3 Identification of the bacterial signature of gastric cancer. Multidimensional analyses were performed considering various combinations of the following bacterial species: Clostridium colicanis (Cc), Fusobacterium canifelinum (Fc), Fusobacterium nucleatum (Fn), Lactobacillus gasseri (Lg), Lactobacillus reuteri (Lr), Megasphaera micronuciformis (Mm), Prevotella intermedia (Pi), Prevotella oris (Po), Streptococcus gordonii (Sg), and Streptococcus parasanguinis (Sp).CC BY · PubMed Central
Figure 4 Evaluation of the discriminatory power of using bacterial abundance as a diagnostic tool. (A) The discriminatory power of the abundance of each individual species C. colicanis, F. canifelinum, F. nucleatum, L. gasseri, and L. reuteri was tested by a receiver operating characteristic curve analysis. (B) The combined abundance of C. colicanis and F. canifelinum (Cc + Fn), C. colicanis, F. canifelinum, and F. nucleatum (Cc + Fc + Fn), and C. colicanis, F. canifelinum, F. nucleatum, Lactobacillus gasseri, and Lactobacillus reuteri (Cc + Fc + Fn + Lg + Lr) was examined.
Figure 4 Evaluation of the discriminatory power of using bacterial abundance as a diagnostic tool. (A) The discriminatory power of the abundance of each individual species C. colicanis, F. canifelinum, F. nucleatum, L. gasseri, and L. reuteri was tested by a receiver operating characteristic curve analysis. (B) The combined abundance of C. colicanis and F. canifelinum (Cc + Fn), C. colicanis, F. canifelinum, and F. nucleatum (Cc + Fc + Fn), and C. colicanis, F. canifelinum, F. nucleatum, Lactobacillus gasseri, and Lactobacillus reuteri (Cc + Fc + Fn + Lg + Lr) was examined.CC BY · PubMed Central

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Metadata

Authors
Hsieh, Y.-Y., Tung, S.-Y., Pan, H.-Y., Yen, C.-W., Xu, H.-W., Lin, Y.-J., Deng, Y.-F., Hsu, W.-T., Wu, C.-S., & Li, C.
Affiliations
  • Chang Gung University (TW)
  • National Chiayi University (TW)
  • Chiayi Chang Gung Memorial Hospital (TW)
  • National Chung Cheng University (TW)
Corresponding author
  • Wu, Cheng-Shyong
  • Li, Chin
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2018-01-09
Volume
8
Issue
1
Pages
158-158
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/s41598-017-18596-0
PMC
PMC5760541
OpenAlex
W2781688166
Cited by
269
References
50
Citations by year
2026: 22 · 2025: 31 · 2024: 39 · 2023: 36 · 2022: 34 · 2021: 45 · 2020: 31 · 2019: 21 · 2018: 10
FWCI
27.80
Type
journal article
Language
en
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
  • Chang Gung Medical Foundation
  • Chiayi Chang Gung Memorial Hospital (CORPG6D0063, CORPG6D0071, CORPG6D0012, CORPG6D0011, CORPG6D0061, CORPG6D0073, CORPG6D0072, CORPG6D0013, CORPG6D0062)
Topics
  • Health Sciences › Medicine › Gastroenterology › Helicobacter pylori-related gastroenterology studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
Keywords
gastric cancer, gastric microbiota, Clostridium, Fusobacterium, Helicobacter pylori, intestinal metaplasia, gastritis, Fusobacterium nucleatum, cancer diagnosis, Lactobacillus, Burkholderia, Enterobacter, Leclercia
MeSH terms
Adult, Aged, Clostridium, Clostridium Infections, Female, Fusobacterium, Fusobacterium Infections, Gastric Mucosa, Humans, Male, Metaplasia, Middle Aged, Neoplasm Staging, RNA, Ribosomal, 16S, Stomach Neoplasms, Taiwan, Young Adult

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