Fecal microbiota transplantation reverses antibiotic and chemotherapy-induced gut dysbiosis in mice
Open accessAbstract
Fecal microbiota transplantation (FMT) is now widely used to treat recurrent Clostridium difficile infection, but has been less studied as a means to restore microbiome diversity and composition following antibiotic or chemotherapy treatments. The purpose of our study was to assess the efficacy of FMT to reverse antibiotic- and chemotherapy-induced gut dysbiosis in a mouse model. C57BL/6J mice were treated with ampicillin for 1 week and/or received a single intraperitoneal injection of 5-Fluorouracil. Fresh stool was collected and analyzed using shotgun metagenomics and the Illumina sequencing platform. Ampicillin caused a significant and immediate decrease in bacterial species richness and diversity that persisted for one week. In mice that received FMT, disruption of the intestinal microbiota was reversed immediately. Antibiotic and chemotherapy administration caused significant alteration in species distribution, including a decrease in the relative proportions of Clostridium scindens and Faecalibacterium prausnitzii, and an increase in known pathogenic species. In mice receiving FMT, we observed a significant increase in species known to exhibit anti-inflammatory properties. Moreover, chemotherapy led to a critical decrease in key 'health-promoting' species and to an altered functional profile, especially when chemotherapy was administered in tandem with antibiotics, and that FMT can ameliorate these effects.
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FMT was reported to decrease antibiotic, chemotherapy, and injury-induced gut permeability and increased the prevalence of specific microbiota strains exhibiting anti-inflammatory properties.
“FMT was reported to decrease antibiotic, chemotherapy, and injury-induced gut permeability and increased the prevalence of specific microbiota strains exhibiting anti-inflammatory properties.195,196”
From Regulation of Intestinal Barrier Function by Microbial Metabolites (2021) · cites this paper as [195]
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- Authors
- Le Bastard, Q., Ward, T., Sidiropoulos, D., Hillmann, B. M., Chun, C. L., Sadowsky, M. J., Knights, D., & Montassier, E.
- Affiliations
- University of Minnesota (US)
- Université de Nantes (FR)
- Corresponding author
- Knights, Dan
- Montassier, Emmanuel
- Venue
- Scientific Reports
- Published
- 2018-04-18
- Volume
- 8
- Issue
- 1
- Pages
- 6219-6219
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC5906603
- OpenAlex
- W2800756579
- Cited by
- 134
- References
- 65
- Citations by year
- 2026: 15 · 2025: 7 · 2024: 16 · 2023: 20 · 2022: 16 · 2021: 26 · 2020: 14 · 2019: 15 · 2018: 5
- FWCI
- 5.33
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, other-oa)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- Société Nationale Française de Gastro-Entérologie
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Medicine › Infectious Diseases › Clostridium difficile and Clostridium perfringens research
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Keywords
- fecal microbiota transplantation, gut dysbiosis, antibiotic-induced dysbiosis, ampicillin, 5-fluorouracil, gut microbiota diversity, Clostridium scindens, Faecalibacterium prausnitzii, anti-inflammatory bacteria, mouse model
- MeSH terms
- Gastrointestinal Microbiome, Fecal Microbiota Transplantation, Animals, Anti-Bacterial Agents, Antineoplastic Agents, Disease Models, Animal, Feces, Biodiversity, Mice, Metagenome, Metagenomics, Dysbiosis
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