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Cited by 64Gut microbiota and healthAnimal Nutrition and PhysiologyGastrointestinal motility and disorders

Depletion of dietary aryl hydrocarbon receptor ligands alters microbiota composition and function

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Brawner, Kyle M., Yeramilli, Venkata A., Duck, Lennard W., Van Der Pol, William, Smythies, Lesley E., Morrow, Casey D., Elson, Charles O., Martin, Colin A.·2019·Scientific Reports
DOI 10.1038/s41598-019-51194-wPMC PMC6789125
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Abstract

The intestinal microbiota is critical for maintaining homeostasis. Dysbiosis, an imbalance in the microbial community, contributes to the susceptibility of several diseases. Many factors are known to influence gut microbial composition, including diet. We have previously shown that fecal immunoglobulin (Ig) A levels are decreased in mice fed a diet free of aryl hydrocarbon receptor (AhR) ligands. Here, we hypothesize this IgA decrease is secondary to diet-induced dysbiosis. We assigned mice to a conventional diet, an AhR ligand-free diet, or an AhR ligand-free diet supplemented with the dietary AhR ligand indole-3-carbinol (I3C). We observed a global alteration of fecal microbiota upon dietary AhR ligand deprivation. Compared to mice on the conventional diet, family Erysipelotrichaceae was enriched in the feces of mice on the AhR ligand-free diet but returned to normal levels upon dietary supplementation with I3C.Faecalibaculum rodentium, an Erysipelotrichaceae species, depleted its growth media of AhR ligands. Cultured fecal bacteria from mice on the AhR ligand-free diet, but not the other two diets, were able to alter IgA levels in vitro, as was F.rodentium alone. Our data point to the critical role of AhR dietary ligands in shaping the composition and proper functioning of gut microbiota.

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  • Environmental differences such as diet, ligand amount in diet, bedding, caging, or facility pathogens can influence observed phenotypes in Ahr null mice.

    “Differences in the environment such as diet, amount of ligand in the diet, bedding, caging, or the presence of pathogens in the facility can influence observed phenotypes.”

    From Rodent Genetic Models of Ah Receptor Signaling (2021) · cites this paper as Brawner et al., 2019

    The Results section (page not provided) shows that diet and dietary AhR ligand supplementation changed fecal microbiota composition in mice. The Methods section (page not provided) describes pathogen-free housing, but the paper does not show that bedding, caging, or facility pathogens influence phenotypes, and it does not establish the claim specifically for Ahr-null mice. (LLM, conf=0.85)

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  • Differences in pathogens can affect immune output in laboratory mice.

    “These environmental considerations are particularly important as it is now known that ligands obtained through the diet can influence microbiome and AHR signaling, and it is also likely that differences in pathogens can affect immune output in laboratory mice (Brawner et al., 2019; Leavy, 2011; Schanz et al., 2020).”

    From Rodent Genetic Models of Ah Receptor Signaling (2021) · cites this paper as Brawner et al., 2019

    The Introduction says pathobionts can stimulate an inflammatory response, and the Results report that microbiota from differently fed mice affect IgA levels. But the mice were maintained under specific pathogen-free conditions (Methods), and the paper does not show that differences in pathogens between laboratory mice alter their immune output. (LLM, conf=0.78)

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  • Dietary ligands can influence the microbiome and AHR signaling.

    “These environmental considerations are particularly important as it is now known that ligands obtained through the diet can influence microbiome and AHR signaling, and it is also likely that differences in pathogens can affect immune output in laboratory mice (Brawner et al., 2019; Leavy, 2011; Schanz et al., 2020).”

    From Rodent Genetic Models of Ah Receptor Signaling (2021) · cites this paper as Brawner et al., 2019

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Authors
Brawner, K. M., Yeramilli, V. A., Duck, L. W., Van Der Pol, W., Smythies, L. E., Morrow, C. D., Elson, C. O., & Martin, C. A.
Affiliations
University of Alabama at Birmingham (US)
Corresponding author
Brawner, Kyle M.
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2019-10-11
Volume
9
Issue
1
Pages
14724-14724
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/s41598-019-51194-w
PMC
PMC6789125
OpenAlex
W2979524708
Cited by
64
References
59
Citations by year
2026: 3 · 2025: 13 · 2024: 11 · 2023: 9 · 2022: 11 · 2021: 11 · 2020: 6
FWCI
2.32
Type
journal article
Language
en
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
U.S. Department of Health & Human Services | NIH | National Center for Advancing Translational Sciences (UL1TR001417)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
  • Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
Keywords
aryl hydrocarbon receptor, gut microbiota, dysbiosis, indole-3-carbinol, Erysipelotrichaceae, Faecalibaculum rodentium, host-microbe interactions
MeSH terms
Firmicutes, Bacillota, Gastrointestinal Microbiome, Animals, Diet, DNA, Ribosomal, Feces, Humans, Immunoglobulin A, Indoles, Ligands, Mice, Inbred C57BL, Receptors, Aryl Hydrocarbon, Dietary Supplements, HCT116 Cells, Mice, Dysbiosis

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