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Cited by 116Probiotics and Fermented FoodsOral microbiology and periodontitis researchReproductive tract infections research

The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index.

Lactobacillus reuteri AN417 cell-free culture supernatant as a novel antibacterial agent targeting oral pathogenic bacteria

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Yang, Kyung Mi, Kim, Ji-Sun, Kim, Hye-Sung, Kim, Young-Youn, Oh, Jeong-Kyu, Jung, Hye-Won, Park, Doo-Sang, Bae, Kwang-Hak·2021·Scientific Reports
DOI 10.1038/s41598-020-80921-xPMC PMC7810884
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Abstract

Lactobacillus reuteri AN417 is a newly characterized probiotic strain. The activity of AN417 against oral pathogenic bacteria is unknown. We investigated the antibacterial activity of cell-free L. reuteri AN417 culture supernatant (LRS) against three oral pathogens: Porphyromonas gingivalis, Fusobacterium nucleatum, and Streptococcus mutans. P. gingivalis and F. nucleatum have been implicated in periodontal disease, whereas S. mutans causes dental caries. Exposing these oral pathogenic bacteria to LRS significantly reduced their growth rates, intracellular ATP levels, cell viability, and time-to-kill. The minimal inhibitory volume of LRS was 10% (v/v) against P. gingivalis, 20% (v/v) for F. nucleatum, and 30% (v/v) for S. mutans. LRS significantly reduced the integrity of biofilms and significantly suppressed the expression of various genes involved in P. gingivalis biofilm formation. The L. reuteri AN417 genome lacked genes encoding reuterin, reuteran, and reutericyclin, which are major antibacterial compounds produced in L. reuteri strains. LRS treated with lipase and α-amylase displayed decreased antibacterial activity against oral pathogens. These data suggest that the antibacterial substances in LRS are carbohydrates and/or fatty acid metabolites. Our results demonstrate that LRS has antimicrobial activity against dental pathogenic bacteria, highlighting its potential utility for the prevention and treatment of P. gingivalis periodontal disease.

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  • Cell-free supernatants of Lactobacillus rhamnosus and Lactobacillus acidophilus can exert an antibiofilm effect by impairing biofilm integrity and reducing the expression of biofilm-associated genes of periodontal pathogens.

    “Cell-free supernatants of Lactobacillus rhamnosus and Lactobacillus acidophilus can exert an antibiofilm effect by impairing biofilm integrity and reducing the expression of the biofilm-associated genes of periodontal pathogens [13,14].”

    From Analysis of Chemical Structure and Antibiofilm Properties of Exopolysaccharides from Lactiplantibacillus plantarum EIR/IF-1 Postbiotics (2022) · cites this paper as [14]

    The Results section ('LRS impedes biofilm formation by the oral pathogenic bacteria'; page unavailable) reports removal of established P. gingivalis biofilms and reduced expression of genes involved in their attachment and formation. But the tested supernatant came from L. reuteri AN417, not the L. rhamnosus or L. acidophilus named in the claim. The source therefore supports the antibiofilm effects, but not their attribution to those two species. (LLM, conf=0.88)

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Metadata

Authors
Yang, K. M., Kim, J.-S., Kim, H.-S., Kim, Y.-Y., Oh, J.-K., Jung, H.-W., Park, D.-S., & Bae, K.-H.
Affiliations
Korea Research Institute of Bioscience and Biotechnology (KR)
Corresponding author
  • Park, Doo-Sang
  • Bae, Kwang-Hak
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2021-01-15
Volume
11
Issue
1
Pages
1631-1631
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/s41598-020-80921-x
PMC
PMC7810884
OpenAlex
W3123964397
Cited by
116
References
43
Citations by year
2026: 19 · 2025: 23 · 2024: 19 · 2023: 25 · 2022: 21 · 2021: 9
FWCI
11.04
Type
journal article
Language
en
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, other-oa)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
National Research Foundation of Korea (NRF) Grant (2019R1F1A1051382, 2016M3A9A5919255)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
  • Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
Keywords
cell-free supernatant, antibacterial activity, oral pathogens, Porphyromonas gingivalis, Fusobacterium nucleatum, Streptococcus mutans, periodontal disease, dental caries, biofilm gene expression, reuterin, probiotics
MeSH terms
alpha-Amylases, Animals, Anti-Bacterial Agents, Culture Media, Fatty Acids, Feces, Humans, Lipase, Microbial Sensitivity Tests, Periodontal Diseases, Phylogeny, Streptococcus mutans, Swine, Porphyromonas gingivalis, Fusobacterium nucleatum, Biofilms, Limosilactobacillus reuteri

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