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Cited by 60Algal biology and biofuel productionMicrobial Metabolic Engineering and BioproductionLipid metabolism and biosynthesis

A multi-omic characterization of temperature stress in a halotolerant Scenedesmus strain for algal biotechnology

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Calhoun, Sara, Bell, Tisza Ann Szeremy, Dahlin, Lukas R., Kunde, Yuliya, LaButti, Kurt, Louie, Katherine B., Kuftin, Andrea, Treen, Daniel +8 more·2021·Communications Biology
DOI 10.1038/s42003-021-01859-yPMC PMC7955037
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Abstract

Abstract Microalgae efficiently convert sunlight into lipids and carbohydrates, offering bio-based alternatives for energy and chemical production. Improving algal productivity and robustness against abiotic stress requires a systems level characterization enabled by functional genomics. Here, we characterize a halotolerant microalgaScenedesmussp. NREL 46B-D3 demonstrating peak growth near 25 °C that reaches 30 g/m2/day and the highest biomass accumulation capacity post cell division reported to date for a halotolerant strain. Functional genomics analysis revealed that genes involved in lipid production, ion channels and antiporters are expanded and expressed. Exposure to temperature stress shifts fatty acid metabolism and increases amino acids synthesis. Co-expression analysis shows that many fatty acid biosynthesis genes are overexpressed with specific transcription factors under cold stress. These and other genes involved in the metabolic and regulatory response to temperature stress can be further explored for strain improvement.

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Authors
Calhoun, S., Bell, T. A. S., Dahlin, L. R., Kunde, Y., LaButti, K., Louie, K. B., Kuftin, A., Treen, D., Dilworth, D., Mihaltcheva, S., Daum, C., Bowen, B. P., Northen, T. R., Guarnieri, M. T., Starkenburg, S. R., & Grigoriev, I. V.
Affiliations
  • Lawrence Berkeley National Laboratory (US)
  • Los Alamos National Laboratory (US)
  • National Laboratory of the Rockies (US)
Corresponding author
  • Starkenburg, Shawn R.
  • Grigoriev, Igor V.
ORCID
Venue
Communications Biology
Publisher
Springer Science and Business Media LLC
Published
2021-03-12
Volume
4
Issue
1
Pages
333-333
ISSN
2399-3642 (ISSN-L)
DOI
10.1038/s42003-021-01859-y
PMC
PMC7955037
OpenAlex
W3134274179
Cited by
60
References
5
Citations by year
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FWCI
1.50
Type
journal article
Language
en
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  • Communications Biology (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • eScholarship (California Digital Library) (submittedVersion, cc-by)
  • OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • eScholarship (California Digital Library) (submittedVersion)
Funders
  • U.S. Department of Energy (-AC02-05CH11231, DE -AC36-08GO28308, Contract No. DE-AC02-05-CH11231, No. DE-AC36-08GO28308, 05CH11231, No. DE-AC02-05-CH11231, DE AC02 05CH11231, NL0032266, 08GO28308, DE-AC36, Contract No. DE-AC36-08GO28308, AC36‐08GO28308, DE-AC02-)
  • Joint Genome Institute (AC02-05CH11231, DE–AC02–05CH11231)
  • Office of Science (No. DE-AC02- 05-CH11231, DEAC02–05CH11231, AC36-08GO28308, AC02-05CH11231, DE-AC02, 05CH11231, Contract No. DE-AC02-05CH11231, DE-AC36-08GO28308)
  • Office of Energy Efficiency and Renewable Energy (AC02-05CH11231, DE- AC02-05CH11231, Contract No. DE-AC36-08GO28308, No. DE-AC02-05CH11231, DE-AC36-08-GO28308., Contract No. DE-AC02-05CH11231, AC36-08-GO28308, NL0032266, No. DE-AC36-08GO28308, NL0032355)
  • Bioenergy Technologies Office (Contract No. DE-AC36-08GO28308, DE-AC36-08-GO28308, DE-AC02- 05CH11231, NL0032266)
  • Office of Energy Efficiency
  • National Renewable Energy Laboratory (Contract No. DE-AC36-08GO28308, DE-AC36-08-GO28308, AC36-08GO28308, DE-AC02-05CH11231)
Topics
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Keywords
temperature stress, halotolerant microalgae, Scenedesmus, lipid production, fatty acid synthesis, ion channels, antiporters, amino acid synthesis, cold stress, transcription factors, gene co-expression, abiotic stress tolerance, algal biotechnology, biomass accumulation
MeSH terms
Amino Acids, Energy Metabolism, Fatty Acids, Gene Expression Regulation, Ion Channels, Temperature, Transcription Factors, Genome, Antiporters, Biomass, Gene Expression Profiling, Scenedesmus, Lipogenesis, Salt Tolerance, Metabolomics, Metabolome, Microalgae, Transcriptome

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

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