Development of a high-productivity, halophilic, thermotolerant microalga Picochlorum renovo
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Abstract Microalgae are promising biocatalysts for applications in sustainable fuel, food, and chemical production. Here, we describe culture collection screening, down-selection, and development of a high-productivity, halophilic, thermotolerant microalga, Picochlorum renovo. This microalga displays a rapid growth rate and high diel biomass productivity (34 g m−2 day−1), with a composition well-suited for downstream processing. P. renovo exhibits broad salinity tolerance (growth at 107.5 g L−1 salinity) and thermotolerance (growth up to 40 °C), beneficial traits for outdoor cultivation. We report complete genome sequencing and analysis, and genetic tool development suitable for expression of transgenes inserted into the nuclear or chloroplast genomes. We further evaluate mechanisms of halotolerance via comparative transcriptomics, identifying novel genes differentially regulated in response to high salinity cultivation. These findings will enable basic science inquiries into control mechanisms governing Picochlorum biology and lay the foundation for development of a microalga with industrially relevant traits as a model photobiology platform.
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Picochlorum renovo is the fastest growing alga, with a doubling time of 2.2 h, which is ~5-10 times faster than many algae.
“The fastest growing alga is Picochlorum renovo that has a double time of just 2.2 h, which is ~5-10 times faster than many algae [28].”
From Key Targets for Improving Algal Biofuel Production (2021) · cites this paper as [28]
The Results (Down-selection, physiology, and compositional analysis; page unavailable) report that P. renovo grew noticeably faster than the other isolates screened, including two Nannochloropsis controls. The paper does not establish that it is the fastest-growing alga overall, or report a 2.2-hour doubling time or a 5–10-fold growth-rate advantage. (LLM, conf=0.85)
Experimental — accuracy not yet measuredCationic starch works over a broader pH range than chitosan.
“Other flocculating agents alternatives include cationic starch [43], which works over a broader range than chitosan [28]”
From Key Targets for Improving Algal Biofuel Production (2021) · cites this paper as [28]
The Methods section (page unavailable) specifies a culture-medium pH of 8.0, but does not discuss flocculants. Searches for cationic starch, chitosan, flocculation, and a comparative pH range found no passage in our copy addressing the claim. (LLM, conf=0.76)
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- Authors
- Dahlin, L. R., Gerritsen, A. T., Henard, C. A., Van Wychen, S., Linger, J. G., Kunde, Y., Hovde, B. T., Starkenburg, S. R., Posewitz, M. C., & Guarnieri, M. T.
- Affiliations
- Colorado School of Mines (US)
- National Laboratory of the Rockies (US)
- Los Alamos National Laboratory (US)
- Corresponding author
- Guarnieri, Michael T.
- ORCID
- Venue
- Communications Biology
- Published
- 2019-10-23
- Volume
- 2
- Issue
- 1
- Pages
- 388-388
- ISSN
- 2399-3642 (ISSN-L)
- PMC
- PMC6811619
- OpenAlex
- W2981832700
- Cited by
- 86
- References
- 67
- Citations by year
- 2026: 11 · 2025: 7 · 2024: 16 · 2023: 15 · 2022: 12 · 2021: 15 · 2020: 10
- FWCI
- 1.97
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Communications Biology (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
- Funders
- U.S. Department of Energy (22000)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Keywords
- halophilic microalgae, salt tolerance, genome sequencing, genetic transformation, chloroplast transformation, transgene expression, comparative transcriptomics, microalgae cultivation
- MeSH terms
- Thermotolerance, Chlorophyta, Biotechnology, Genetic Engineering, Industrial Microbiology, Biomass, Gene Expression Profiling, Phototrophic Processes, Genome, Chloroplast, Salt Tolerance, Biocatalysis, Microalgae, Genome, Microbial
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