Oxygen and Mammalian Cell Culture: Are We Repeating the experiment of Dr. Ox?

Ast, Tslil; Mootha, Vamsi K.

doi:10.1038/s42255-019-0105-0

Cited by 125Cancer, Hypoxia, and MetabolismRedox biology and oxidative stressViral Infectious Diseases and Gene Expression in Insects

Oxygen and Mammalian Cell Culture: Are We Repeating the experiment of Dr. Ox?

Ast, Tslil, Mootha, Vamsi K.·2019·Nature Metabolism

DOI 10.1038/s42255-019-0105-0

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In extreme cases, variations in culture conditions may resemble levels consistent with hypercapnia and hypoxia rather than conditions typical of in vivo extracellular fluids, although in vivo environments vary considerably among selected tissues.
“In such extreme cases, variations in culture conditions may resemble levels consistent with hypercapnia and hypoxia rather than conditions typical of in vivo extracellular fluids, although in vivo environments vary considerably among selected tissues (Ast and Mootha, 2019).”
From Toward Best Practices for Controlling Mammalian Cell Culture Environments (2022) · cites this paper as Ast and Mootha, 2019
O2 levels vary across human tissues and range from 13% O2 in the lung-pulmonary vein to 1-3% O2 in the uterus.
“For instance, O2 levels vary across human tissues and range from 13% O2 in the lung-pulmonary vein to 1–3% O2 in the uterus (Ast and Mootha, 2019).”
From Toward Best Practices for Controlling Mammalian Cell Culture Environments (2022) · cites this paper as Ast and Mootha, 2019
Considering how niche in vivo environments affect experimental outcomes could increase the robustness of experiments and the likelihood that findings have relevance to focal in vivo compartments.
“By considering how niche in vivo environments affect experimental outcomes, researchers could further increase the robustness of their experiments and increase the likelihood that findings have relevance to focal in vivo compartments (Ast and Mootha, 2019).”
From Toward Best Practices for Controlling Mammalian Cell Culture Environments (2022) · cites this paper as Ast and Mootha, 2019
Reliable reports of environmental parameters within selected human tissues are presently limited.
“although reliable reports of environmental parameters within selected human tissues are presently limited (Al-Ani et al., 2018; Ast and Mootha, 2019)”
From Toward Best Practices for Controlling Mammalian Cell Culture Environments (2022) · cites this paper as Ast and Mootha, 2019

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Authors: Ast, T., & Mootha, V. K.
Affiliations: Broad Institute (US)
Corresponding author: Mootha, Vamsi K.
ORCID: Ast, Tslil
Mootha, Vamsi K.
Venue: Nature Metabolism
Publisher: Springer Science and Business Media LLC
Published: 2019-08-05
Volume: 1
Issue: 9
Pages: 858-860
ISSN: 2522-5812 (ISSN-L)
DOI: 10.1038/s42255-019-0105-0
OpenAlex: W2966730488
Cited by: 125
References: 20
Citations by year: 2026: 7 · 2025: 20 · 2024: 23 · 2023: 13 · 2022: 27 · 2021: 20 · 2020: 13 · 2019: 1
FWCI: 4.83
Type: journal article
Language: en
Open access: closed
Licence: www.springernature.com/gp/researchers/text-and-data-mining (tdm)
www.springernature.com/gp/researchers/text-and-data-mining (vor)
Locations: Nature Metabolism (publishedVersion)
PubMed (publishedVersion)
Funders: U.S. Department of Health & Human Services | National Institutes of Health (R35GM122455)
Howard Hughes Medical Institute
Marriot Foundation
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Hypoxia, and Metabolism
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
Keywords: oxygen, mammalian cell culture, reproducibility, hypoxia, experimental interpretation
MeSH terms: Animals, Culture Media, Mammals, Oxygen, Research

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