Effects of intracerebroventricular leptin administration on food intake, body weight gain and diencephalic nitric oxide synthase activity in the mouse
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1. Intracranial administration of leptin reduces both food intake and body weight gain in the mouse. Inhibitors of nitric oxide (NO) synthase produce similar effects. 2. To investigate the role of the brain L-arginine/NO pathway in mediating this effect of leptin, we have evaluated food intake and body weight gain after daily (5 days) intracerebroventricular (i.c.v.) administration of leptin (0.5-2 microg) alone or in association with L-arginine (10 microg). Moreover, we measured diencephalic nitric oxide synthase (NOS) activity after a single i.c.v. leptin (0.25-2 microg) injection and after consecutive doses of leptin (0.25-2 microg) over 5 days. The time course of the effect of leptin on NOS activity was also evaluated. 3. I.c.v. injected leptin (1 and 2 microg) significantly and dose-dependently reduced food intake and body weight gain with respect to vehicle (food intake: 5.97+/-0.16 g 24 h(-1) and 4.27+/-0.18 g 24 h(-1), respectively, vs 8.05+/-0.34 g 24 h(-1), P<0.001, n=6 for each group; body weight gain: -10.7+/-0.46% and -15.7+/-0.65%, respectively, vs 5.14+/-0.38%, P<0.001, n=6 for each group). This effect was antagonized by L-arginine (food intake: 7.90+/-0.37 g 24 h; body weight gain: 5.11+/-0.31%, n=6). Diencephalic NOS activity was significantly reduced by the highest doses of leptin with respect to vehicle (vehicle: 0.90+/-0.04 nmol citrulline min(-1) g(-1) tissue; leptin 1 microg: 0.62+/-0.03 nmol citrulline min(-1) g(-1) tissue, P<0.001; leptin 2 microg: 0.44+/-0.03 nmol citrulline min(-1) g(-1) tissue, P<0.001, n=6 for each group). Similar results were obtained in animals treated with daily consecutive doses of leptin. The inhibitory effect appeared rapidly (within 30 min) and was long lasting (up to 12 h). 4. Our results suggest that the brain L-arginine/NO pathway may be involved in the central effect of leptin on feeding behaviour and body weight gain in mice.
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Intracranial leptin diminishes diencephalic NOS activity and decreases food intake and body weight gain in rodents.
“Leptin, given intracranially, was found to diminish diencephalic NOS activity and decrease food intake and body weight gain, and intracranial co-administration of L-arginine antagonized this effect [255].”
From Regulation of obesity and insulin resistance by nitric oxide (2014) · cites this paper as [255]
Intracranial co-administration of L-arginine antagonizes leptin's effect on food intake and body weight gain.
“Leptin, given intracranially, was found to diminish diencephalic NOS activity and decrease food intake and body weight gain, and intracranial co-administration of L-arginine antagonized this effect [255].”
From Regulation of obesity and insulin resistance by nitric oxide (2014) · cites this paper as [255]
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Cites
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302 · see citing works
Positional cloning of the mouse obese gene and its human homologue
Zhang, Y., Proenca, R., Maffei, M., Barone, M., Leopold, L., & Friedman, J. M. (1994) · Nature · doi:10.1038/372425a0 · Cited by 13,419 · see citing works
Effects of the <i>obese</i> Gene Product on Body Weight Regulation in <i>ob</i> / <i>ob</i> Mice
Pelleymounter, M. A., Cullen, M. J., Baker, M. B., Hecht, R., Winters, D., Boone, T., & Collins, F. (1995) · Science · doi:10.1126/science.7624776 · Cited by 4,017 · see citing works
Localization of nitric oxide synthase indicating a neural role for nitric oxide
Bredt, D. S., Hwang, P. M., & Snyder, S. H. (1990) · Nature · doi:10.1038/347768a0 · Cited by 2,981 · see citing works
Nitric oxide synthase and neuronal NADPH diaphorase are identical in brain and peripheral tissues.
Dawson, T. M., Bredt, D. S., Fotuhi, M., Hwang, P. M., & Snyder, S. H. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.17.7797 · Cited by 1,843 · see citing works
Identification of targets of leptin action in rat hypothalamus.
Schwartz, M. W., Seeley, R. J., Campfield, L. A., Burn, P., & Baskin, D. G. (1996) · Journal of Clinical Investigation · doi:10.1172/jci118891 · Cited by 1,475 · see citing works
Knowles, R. G., Palacios‐Callender, M., Palmer, R. M. J., & Moncada, S. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.13.5159 · Cited by 1,431 · see citing works
Nitric Oxide: A Physiologic Messenger
Lowenstein, C. J., Dinerman, J. L., & Snyder, S. H. (1994) · Annals of Internal Medicine · doi:10.7326/0003-4819-120-3-199402010-00009 · Cited by 913 · see citing works
Attenuation of the Obesity Syndrome of ob/ob Mice by the Loss of Neuropeptide Y
Erickson, J. C., Hollopeter, G., & Palmiter, R. D. (1996) · Science · doi:10.1126/science.274.5293.1704 · Cited by 794 · see citing works
Salter, M., Knowles, R. G., & Moncada, S. (1991) · FEBS Letters · doi:10.1016/0014-5793(91)81123-p · Cited by 630 · see citing works
Nitric Oxide in the Nervous System
Zhang, J., & Snyder, S. H. (1995) · The Annual Review of Pharmacology and Toxicology · doi:10.1146/annurev.pa.35.040195.001241 · Cited by 512 · see citing works
The role of nitric oxide in hippocampal long-term potentiation
Haley, J. E., Wilcox, G. L., & Chapman, P. F. (1992) · Neuron · doi:10.1016/0896-6273(92)90288-o · Cited by 470 · see citing works
Role of NO Production in NMDA Receptor-Mediated Neurotransmitter Release in Cerebral Cortex
Montague, P. R., Gancayco, C. D., Winn, M. J., Marchase, R. B., & Friedlander, M. J. (1994) · Science · doi:10.1126/science.7508638 · Cited by 466 · see citing works
Nitric oxide synthase: expression and expressional control of the three isoforms
F�rstermann, U., & Kleinert, H. (1995) · Naunyn-Schmiedeberg s Archives of Pharmacology · doi:10.1007/bf00172772 · Cited by 462 · see citing works
Leptin Activates Neurons in Ventrobasal Hypothalamus and Brainstem
Elmquist, J. K., Ahima, R. S., Maratos–Flier, E., Flier, J. S., & Saper, C. B. (1997) · Endocrinology · doi:10.1210/endo.138.2.5033 · Cited by 438 · see citing works
Yu, W. H., Walczewska, A., Karanth, S., & McCann, S. M. (1997) · Endocrinology · doi:10.1210/endo.138.11.5649 · Cited by 234 · see citing works
Leptin expression and action: New experimental paradigms
Flier, J. S. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.9.4242 · Cited by 223 · see citing works
Evidence that nitric oxide modulates food intake in mice
Morley, J. E., & Flood, J. F. (1991) · Life Sciences · doi:10.1016/0024-3205(91)90102-h · Cited by 188 · see citing works
Costa, A. C., Trainer, P. J., Besser, M., & Grossman, A. B. (1993) · Brain Research · doi:10.1016/0006-8993(93)91739-f · Cited by 174 · see citing works
Leptin: its role in obesity and beyond
Tritos, N. A., & Mantzoros, C. S. (1997) · Diabetologia · doi:10.1007/s001250050838 · Cited by 167 · see citing works
Inhibition of Constitutive and Inducible Nitric Oxide Synthase: Potential Selective Inhibition
Fukuto, J. M., & Chaudhuri, G. (1995) · The Annual Review of Pharmacology and Toxicology · doi:10.1146/annurev.pa.35.040195.001121 · Cited by 156 · see citing works
OB-Rb gene transfer to leptin-resistant islets reverses diabetogenic phenotype
Wang, M.-Y., Koyama, K., Shimabukuro, M., Newgard, C. B., & Unger, R. H. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.2.714 · Cited by 102 · see citing works
Squadrito, F., Calapai, G., Cucinotta, D., Altavilla, D., Zingarelli, B., Ioculano, M., Urna, G., Sardella, A., Campo, G. M., & Caputi, A. P. (1993) · European Journal of Pharmacology · doi:10.1016/0014-2999(93)90422-e · Cited by 99 · see citing works
Self-citationEvidence that nitric oxide modulates drinking behaviour
Calapai, G., Squadrito, F., Altavilla, D., Zingarelli, B., Campo, G. M., Cilia, M., & Caputi, A. P. (1992) · Neuropharmacology · doi:10.1016/0028-3908(92)90038-q · Cited by 85 · see citing works
Self-citationSquadrito, F., Calapai, G., Altavilla, D., Cucinotta, D., Zingarelli, B., Arcoraci, V., Campo, G. M., & Caputi, A. P. (1994) · European Journal of Pharmacology · doi:10.1016/0014-2999(94)90081-7 · Cited by 61 · see citing works
Self-citationStricker‐Krongrad, A., Beck, B., & Burlet, C. R. (1995) · Life Sciences · doi:10.1016/0024-3205(95)02260-0 · Cited by 48 · see citing works
Sarro, G. D., Ascioti, C., Paola, E. D. D., et al. (1992) · General Pharmacology The Vascular System · doi:10.1016/0306-3623(92)90313-9 · Cited by 34 · see citing works
Calapai, G., Mazzaglia, G., Cilia, M., Zingarelli, B., Squadrito, F., & Caputi, A. P. (1994) · British Journal of Pharmacology · doi:10.1111/j.1476-5381.1994.tb14890.x · Cited by 28 · see citing works
Self-citationNitric oxide and drinking behaviour
Calapai, G., & Caputi, A. P. (1996) · Regulatory Peptides · doi:10.1016/0167-0115(96)00050-x · Cited by 27 · see citing works
Self-citationTakakura, K., Goto, Y., & Muramatsu, I. (1995) · Life Sciences · doi:10.1016/0024-3205(95)02250-3 · Cited by 24 · see citing works
Wahle, P., Müller, T. H., & Swandulla, D. (1993) · Brain Research · doi:10.1016/0006-8993(93)91774-m · Cited by 12 · see citing works
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Regulation of obesity and insulin resistance by nitric oxide
Sansbury, B. E., & Hill, B. G. (2014) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2014.05.016 · Cited by 274 · see citing works
“body weight gain, and intracranial coadministration of L-arginine antagonized this effect [255].”
BackgroundInhibition of NO Synthesis Enhances Chronic Cardiovascular and Renal Actions of Leptin
Kuo, J. J., Jones, O. B., & Hall, J. E. (2001) · Hypertension · doi:10.1161/01.hyp.37.2.670 · Cited by 114 · see citing works
Ciriello, J., & Moreau, J. M. (2012) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.00068.2012 · Cited by 33 · see citing works
- Authors
- Calapai, G., Corica, F., Allegra, A., Corsonello, A., Sautebin, L., De Gregorio, T., Di Rosa, M., Costantino, G., Buemi, M., & Caputi, A. P.
- Affiliations
- University of Messina (IT)
- University of Naples Federico II (IT)
- Corresponding author
- Calapai, Gioacchino
- Venue
- British Journal of Pharmacology
- Publisher
- Wiley
- Published
- 1998-10
- Volume
- 125
- Issue
- 4
- Pages
- 798-802
- ISSN
- 0007-1188 (ISSN-L)
- 1476-5381
- PMC
- PMC1571026
- OpenAlex
- W2047614189
- Cited by
- 56
- References
- 31
- Citations by year
- 2026: 1 · 2021: 1 · 2020: 1 · 2018: 2 · 2016: 1 · 2015: 2 · 2014: 6 · 2013: 3 · 2012: 3
- FWCI
- 1.94
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- British Journal of Pharmacology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Archive of Doctoral Theses and Digital Collections (University of Calabria) (submittedVersion)
- Topics
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Regulation of Appetite and Obesity
- Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
- Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
- Keywords
- leptin, dietary intake, weight gain, nitric oxide synthase, L-arginine, L-arginine-nitric oxide pathway, diencephalon, intracerebroventricular administration, appetite regulation, neuroendocrine regulation of feeding, mice, nitric oxide synthase inhibitors, dose-response relationship
- MeSH terms
- Animals, Arginine, Body Weight, Brain, Diencephalon, Drug Interactions, Eating, Injections, Intraventricular, Male, Obesity, Proteins, Time Factors, Nitric Oxide Synthase, Leptin, Mice
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