Cited by 84Virus-based gene therapy researchNeurogenesis and neuroplasticity mechanismsBarrier Structure and Function Studies

Adeno-associated virus type 4 (AAV4) targets ependyma and astrocytes in the subventricular zone and RMS

Liu, G, Martins, I H, Chiorini, J A, Davidson, B L·2005·Gene Therapy

Abstract

The subventricular zone (SVZ) is one of the neurogenic niches in the adult mammalian brain. The SVZ is of interest for studies on neurogenesis and stem cell therapy. Here, we report specific transduction of ependyma and/or astrocytes by recombinant adeno-associated virus type 4 (AAV4) viral vectors. AAV4 vectors encoding beta-galactosidase or eGFP were injected into the lateral ventricles of neonatal and adult C57BL/6 mouse brains. In addition, SVZ injections were conducted on adult mice. AAV4 vectors show a characteristic transduction of the ependyma independent of delivery route. However, AAV4 virus injected into the SVZ targeted GFAP positive astrocytes forming the glial tube in the SVZ and rostral migratory stream (RMS). Our results introduce AAV4 as a new tool by which to manipulate glial cells in the RMS.

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Crossref · 10.1038/sj.gt.3302554

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Authors
Liu, G., Martins, I. H., Chiorini, J. A., & Davidson, B. L.
Affiliations
University of Iowa (US)
Venue
Gene Therapy
Published
2005-06-02
Volume
12
Issue
20
Pages
1503-1508
ISSN
  • 0969-7128 (ISSN-L)
  • 1476-5462
OpenAlex
W2044333152
Cited by
84
References
30
Citations by year
2026: 2 · 2025: 2 · 2024: 2 · 2023: 2 · 2022: 3 · 2021: 7 · 2020: 3 · 2019: 4 · 2018: 6 · 2017: 3 · 2016: 4 · 2015: 4 · 2014: 3 · 2013: 8 · 2012: 5
FWCI
2.16
Type
journal article
Language
en
Open access
closed
Locations
  • Gene Therapy (publishedVersion)
  • PubMed (publishedVersion)
Funders
Eunice Kennedy Shriver National Institute of Child Health and Human Development (HD-33531, R01HD033531)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
Keywords
subventricular zone, rostral migratory stream, ependyma, astrocytes, viral transduction, lateral ventricle injection, neurogenesis, lacZ reporter, EGFP reporter
MeSH terms
Dependovirus, Animals, Animals, Newborn, Astrocytes, Cell Movement, Ependyma, Genetic Vectors, Immunohistochemistry, Injections, Neural Crest, Neuroglia, Transduction, Genetic, Genetic Therapy, Gene Expression, Lateral Ventricles, Green Fluorescent Proteins, Mice

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