Adeno-associated virus type 4 (AAV4) targets ependyma and astrocytes in the subventricular zone and RMS
Abstract
The subventricular zone (SVZ) is one of the neurogenic niches in the adult mammalian brain. The SVZ is of interest for studies on neurogenesis and stem cell therapy. Here, we report specific transduction of ependyma and/or astrocytes by recombinant adeno-associated virus type 4 (AAV4) viral vectors. AAV4 vectors encoding beta-galactosidase or eGFP were injected into the lateral ventricles of neonatal and adult C57BL/6 mouse brains. In addition, SVZ injections were conducted on adult mice. AAV4 vectors show a characteristic transduction of the ependyma independent of delivery route. However, AAV4 virus injected into the SVZ targeted GFAP positive astrocytes forming the glial tube in the SVZ and rostral migratory stream (RMS). Our results introduce AAV4 as a new tool by which to manipulate glial cells in the RMS.
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AAV4 is astrocyte and ependymal cell specific.
“AAV4 is astrocyte and ependymal cell specific”
From AAVrh.10-Mediated APOE2 Central Nervous System Gene Therapy for APOE4-Associated Alzheimer's Disease (2018) · cites this paper as [70]
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- Authors
- Liu, G., Martins, I. H., Chiorini, J. A., & Davidson, B. L.
- Affiliations
- University of Iowa (US)
- Venue
- Gene Therapy
- Published
- 2005-06-02
- Volume
- 12
- Issue
- 20
- Pages
- 1503-1508
- ISSN
- 0969-7128 (ISSN-L)
- 1476-5462
- OpenAlex
- W2044333152
- Cited by
- 84
- References
- 30
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 2 · 2023: 2 · 2022: 3 · 2021: 7 · 2020: 3 · 2019: 4 · 2018: 6 · 2017: 3 · 2016: 4 · 2015: 4 · 2014: 3 · 2013: 8 · 2012: 5
- FWCI
- 2.16
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Gene Therapy (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (HD-33531, R01HD033531)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
- Keywords
- subventricular zone, rostral migratory stream, ependyma, astrocytes, viral transduction, lateral ventricle injection, neurogenesis, lacZ reporter, EGFP reporter
- MeSH terms
- Dependovirus, Animals, Animals, Newborn, Astrocytes, Cell Movement, Ependyma, Genetic Vectors, Immunohistochemistry, Injections, Neural Crest, Neuroglia, Transduction, Genetic, Genetic Therapy, Gene Expression, Lateral Ventricles, Green Fluorescent Proteins, Mice
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