Hair follicle regeneration using grafted rodent and human cells
Open accessAbstract
Hair follicle regeneration involves epithelial-mesenchymal interactions (EMIs) of follicular epithelial and dermal papilla (DP) cells. Co-grafting of those cellular components from mice allows complete hair reconstitution. However, regeneration of human hair in a similar manner has not been reported. Here, we investigated the possibility of cell-based hair generation from human cells. We found that DP-enriched cells (DPE) are more critical than epidermal cells in murine hair reconstitution on a cell number basis, and that murine DPE are also competent for hair regeneration with rat epidermal cells. Co-grafting of human keratinocytes derived from neonatal foreskins with murine DPE produced hair follicle-like structures consisting of multiple epidermal cell layers with a well-keratinized innermost region. Those structures expressed hair follicle-specific markers including hair keratin, and markers expressed during developmental stages. However, the lack of regular hair structures indicates abnormal folliculogenesis. Similar hair follicle-like structures were also generated with cultured human keratinocytes after the first passage, or with keratinocytes derived from adult foreskins, demonstrating that epidermal cells even at a mature stage can differentiate in response to inductive signals from DP cells. This study emphasizes the importance of EMI in follicular generation and the differentiation potential of epidermal keratinocytes.
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To date, investigation into generating skin in vitro has focused on inducing pure populations of skin cell types from mouse and human PSCs.
“To date, investigation into generating skin in vitro have focused on inducing pure populations of skin cell types—e.g. dermal fibroblasts or keratinocytes—from mouse and human PSCs (Coraux et al., 2003; Ehama et al., 2007; Guenou et al., 2009; Itoh et al., 2013; 2011; Mavilio et al., 2006).”
From Hair Follicle Development in Mouse Pluripotent Stem Cell-Derived Skin Organoids (2018) · cites this paper as Ehama et al., 2007
The Human keratinocyte culture section says the keratinocytes were isolated from neonatal or adult foreskins, not induced from pluripotent stem cells. The Introduction and Discussion describe combining human epidermal cells with murine dermal papilla cells by grafting to generate hair follicle-like structures, rather than generating skin in vitro from PSC-derived cell populations. Our copy provides no page numbers for these sections. (LLM, conf=0.86)
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Cites
Molecular Mechanisms Regulating Hair Follicle Development
Millar, S. E. (2002) · Journal of Investigative Dermatology · doi:10.1046/j.0022-202x.2001.01670.x · Cited by 967 · see citing works
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Sox2-positive dermal papilla cells specify hair follicle type in mammalian epidermis
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- Authors
- Ehama, R., Ishimatsu-Tsuji, Y., Iriyama, S., Ideta, R., Soma, T., Yano, K., Kawasaki, C., Suzuki, S., Shirakata, Y., Hashimoto, K., & Kishimoto, J.
- Affiliations
- Shiseido Group (Japan) (JP)
- Ehime University Hospital (JP)
- Corresponding author
- Kishimoto, Jiro
- Venue
- Journal of Investigative Dermatology
- Publisher
- Elsevier BV
- Published
- 2007-09
- Volume
- 127
- Issue
- 9
- Pages
- 2106-2115
- ISSN
- 0022-202X (ISSN-L)
- OpenAlex
- W2038128569
- Cited by
- 110
- References
- 1
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 4 · 2023: 3 · 2022: 5 · 2021: 9 · 2020: 10 · 2019: 3 · 2018: 10 · 2017: 7 · 2016: 7 · 2015: 4 · 2014: 7 · 2013: 11 · 2012: 3
- FWCI
- 5.87
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Journal of Investigative Dermatology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education, Culture, Sports, Science and Technology
- Ministry of Health, Labour and Welfare
- Topics
- Health Sciences › Medicine › Urology › Hair Growth and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Skin and Cellular Biology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › dental development and anomalies
- Keywords
- hair follicle regeneration, dermal papilla cells, epithelial-mesenchymal interactions, co-grafting, human keratinocytes, folliculogenesis, hair keratin, epidermal cells
- MeSH terms
- Animals, Epidermis, Epithelial Cells, Epithelium, Humans, Keratins, Mesoderm, Mice, Inbred ICR, Mice, Nude, Mice, Transgenic, Regeneration, Keratinocytes, Hair Follicle, Mice, Rats
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