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Roles of the Rac1 and Rac3 GTPases in human tumor cell invasion

Chan, Amanda Y, Coniglio, Salvatore J, Chuang, Ya-yu, Michaelson, David, Knaus, Ulla G, Philips, Mark R, Symons, Marc·2005·Oncogene
DOI 10.1038/sj.onc.1208909
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Abstract

Members of the Rho family of small GTPases have been shown to be involved in tumorigenesis and metastasis. Currently, most of the available information on the function of Rho proteins in malignant transformation is based on the use of dominant-negative mutants of these GTPases. The specificity of these dominant-negative mutants is limited however. In this study, we used small interfering RNA directed against either Rac1 or Rac3 to reduce their expression specifically. In line with observations using dominant-negative Rac1 in other cell types, we show that RNA interference-mediated depletion of Rac1 strongly inhibits lamellipodia formation, cell migration and invasion in SNB19 glioblastoma cells. Surprisingly however, Rac1 depletion has a much smaller inhibitory effect on SNB19 cell proliferation and survival. Interestingly, whereas depletion of Rac3 strongly inhibits SNB19 cell invasion, it does not affect lamellipodia formation and has only minor effects on cell migration and proliferation. Similar results were obtained in BT549 breast carcinoma cells. Thus, functional analysis of Rac1 and Rac3 using RNA interference reveals a critical role for these GTPases in the invasive behavior of glioma and breast carcinoma cells.

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Research works printed but not linked: 45

  1. Allen WE, Zicha D, Ridley AJ and Jones GE . (1998). J. Cell. Biol., 141, 1147–1157. · DOI
  2. Anand-Apte B, Zetter BR, Viswanathan A, Qiu RG, Chen J, Ruggieri R and Symons M . (1997). J. Biol. Chem., 272, 30688–30692. · DOI
  3. Bokoch GM . (2003). Annu. Rev. Biochem., 72, 743–781. · DOI
  4. Bouzahzah B, Albanese C, Ahmed F, Pixley F, Lisanti MP, Segall JD, Condeelis J, Joyce D, Minden A, Der CJ, Chan A, Symons M and Pestell RG . (2001). Mol. Med., 7, 816–830. · DOI
  5. Burridge K and Wennerberg K . (2004). Cell, 116, 167–179. · DOI · Paper page
  6. Chuang YY, Tran NL, Rusk N, Nakada M, Berens ME and Symons M . (2004). Cancer Res., 64, 8271–8275. · DOI
  7. Coniglio SJ, Jou TS and Symons M . (2001). J. Biol. Chem., 276, 28113–28120. · DOI
  8. Debreceni B, Gao Y, Guo F, Zhu K, Jia B and Zheng Y . (2004). J. Biol. Chem., 279, 3777–3786. · DOI
  9. Denli AM and Hannon GJ . (2003). Trends Biochem. Sci., 28, 196–201. · DOI
  10. Dykxhoorn DM, Novina CD and Sharp PA . (2003). Nat. Rev. Mol. Cell. Biol., 4, 457–467. · DOI
  11. Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K and Tuschl T . (2001). Nature, 411, 494–498. · DOI · Paper page
  12. Feig LA . (1999). Nat. Cell. Biol., 1, E25–E27. · DOI
  13. Filippi MD, Harris CE, Meller J, Gu Y, Zheng Y and Williams DA . (2004). Nat. Immunol., 5, 744–751. · DOI · Paper page
  14. Gu Y, Filippi MD, Cancelas JA, Siefring JE, Williams EP, Jasti AC, Harris CE, Lee AW, Prabhakar R, Atkinson SJ, Kwiatkowski DJ and Williams DA . (2003). Science, 302, 445–449. · DOI · Paper page
  15. Gulli MP and Peter M . (2001). Genes Dev., 15, 365–379. · DOI
  16. Haataja L, Groffen J and Heisterkamp N . (1997). J. Biol. Chem., 272, 20384–20388. · DOI · Paper page
  17. Haataja L, Kaartinen V, Groffen J and Heisterkamp N . (2002). J. Biol. Chem., 277, 8321–8328. · DOI
  18. Joneson T, White MA, Wigler MH and Bar-Sagi D . (1996). Science, 271, 810–812. · DOI
  19. Karnoub AE, Worthylake DK, Rossman KL, Pruitt WM, Campbell SL, Sondek J and Der CJ . (2001). Nat. Struct. Biol., 8, 1037–1041. · DOI · Paper page
  20. Keely PJ, Westwick JK, Whitehead IP, Der CJ and Parise LV . (1997). Nature, 390, 632–636. · DOI
  21. Kheradmand F, Werner E, Tremble P, Symons M and Werb Z . (1998). Science, 280, 898–902. · DOI
  22. Khosravi-Far R, Solski PA, Clark GJ, Kinch MS and Der CJ . (1995). Mol. Cell. Biol., 15, 6443–6453. · DOI · Paper page
  23. Lin R, Bagrodia S, Cerione R and Manor D . (1997). Curr. Biol., 7, 794–797. · DOI
  24. Michaelson D, Silletti J, Murphy G, D'Eustachio P, Rush M and Philips MR . (2001). J. Cell. Biol., 152, 111–126. · DOI · Paper page
  25. Mira JP, Benard V, Groffen J, Sanders LC and Knaus UG . (2000). Proc. Natl. Acad. Sci. USA, 97, 185–189. · DOI
  26. Nobes CD and Hall A . (1999). J. Cell. Biol., 144, 1235–1244. · DOI
  27. O'Connor KL, Nguyen BK and Mercurio AM . (2000). J. Cell. Biol., 148, 253–258. · DOI
  28. Qiu RG, Abo A, McCormick F and Symons M . (1997). Mol. Cell. Biol., 17, 3449–3458. · DOI
  29. Qiu RG, Chen J, Kirn D, McCormick F and Symons M . (1995a). Nature, 374, 457–459. · DOI
  30. Qiu RG, Chen J, McCormick F and Symons M . (1995b). Proc. Natl. Acad. Sci. USA, 92, 11781–11785. · DOI · Paper page
  31. Raftopoulou M and Hall A . (2004). Dev. Biol., 265, 23–32. · DOI · Paper page
  32. Ridley AJ, Paterson HF, Johnston CL, Diekmann D and Hall A . (1992). Cell, 70, 401–410. · DOI
  33. Ridley AJ, Schwartz MA, Burridge K, Firtel RA, Ginsberg MH, Borisy G, Parsons JT and Horwitz AR . (2003). Science, 302, 1704–1709. · DOI
  34. Ruggieri R, Chuang YY and Symons M . (2001). Mol. Med., 7, 293–300. · DOI
  35. Sahai E and Marshall CJ . (2002). Nat. Rev. Cancer, 2, 133–142. · DOI · Paper page
  36. Saxena S, Jonsson ZO and Dutta A . (2003). J. Biol. Chem., 278, 44312–44319. · DOI
  37. Scacheri PC, Rozenblatt-Rosen O, Caplen NJ, Wolfsberg TG, Umayam L, Lee JC, Hughes CM, Shanmugam KS, Bhattacharjee A, Meyerson M and Collins FS . (2004). Proc. Natl. Acad. Sci. USA, 101, 1892–1897. · DOI
  38. Shaw LM, Rabinovitz I, Wang HH, Toker A and Mercurio AM . (1997). Cell, 91, 949–960. · DOI
  39. Skehan P, Storeng R, Scudiero D, Monks A, McMahon J, Vistica D, Warren JT, Bokesch H, Kenney S and Boyd MR . (1990). J. Natl. Cancer Inst., 82, 1107–1112. · DOI
  40. Small JV, Stradal T, Vignal E and Rottner K . (2002). Trends Cell Biol., 12, 112–120. · DOI
  41. Symons M and Settleman J . (2000). Trends Cell Biol., 10, 415–419. · DOI · Paper page
  42. Van Aelst L and D'Souza-Schorey C . (1997). Genes Dev., 11, 2295–2322. · DOI
  43. Wells CM, Walmsley M, Ooi S, Tybulewicz V and Ridley AJ . (2004). J. Cell. Sci., 117, 1259–1268. · DOI
  44. Zheng Y . (2001). Trends Biochem. Sci., 26, 724–732. · DOI · Paper page
  45. Zhuge Y and Xu J . (2001). J. Biol. Chem., 276, 16248–16256. · DOI

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Metadata

Authors
Chan, A. Y., Coniglio, S. J., Chuang, Y.-Y., Michaelson, D., Knaus, U. G., Philips, M. R., & Symons, M.
Venue
Oncogene
Publisher
Springer Science and Business Media LLC
Published
2005-07-18
Volume
24
Issue
53
Pages
7821-7829
ISSN
  • 0950-9232
  • 1476-5594
DOI
10.1038/sj.onc.1208909
Cited by
202
References
2
Language
en
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CoChat. (2026). Citation check: Roles of the Rac1 and Rac3 GTPases in human tumor cell invasion. https://app.cochat.ai/papers/10.1038/sj.onc.1208909
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Chan, A. Y., Coniglio, S. J., Chuang, Y.-Y., Michaelson, D., Knaus, U. G., Philips, M. R., & Symons, M. (2005). Roles of the Rac1 and Rac3 GTPases in human tumor cell invasion. Oncogene. https://doi.org/10.1038/sj.onc.1208909