The DOI matches its Crossref record; 63 of the 64 references it cites were found in a registry or an index.
Tumor suppressor Pdcd4 inhibits invasion/intravasation and regulates urokinase receptor (u-PAR) gene expression via Sp-transcription factors
Abstract
Tumor suppressor Pdcd4 has recently been shown to inhibit invasion by activating activator protein-1 (AP-1); however, little is known of the functionally significant Pdcd4-target genes. The urokinase receptor (u-PAR) promotes invasion/metastasis, and is associated with poor cancer-patient survival. The present study was conducted (1) to investigate a role for Pdcd4 in intravasation, invasion and u-PAR regulation, and (2) to describe mechanisms by which this is achieved. Fourteen cell lines showed reciprocal expression of u-PAR/Pdcd4. Resected tumor/normal tissues of 29 colorectal cancer patients demonstrated a significant inverse correlation between Pdcd4/u-PAR. siRNA-Pdcd4-transfected GEO cells significantly increased endogenous u-PAR mRNA/protein. A u-PAR-promoter-chloramphenicol acetyl transferase (CAT)-reporter was reduced in activity with increasing Pdcd4 expression in RKO. Deletion of a putative Sp-1-binding site (-402/-350) inhibited u-PAR promoter regulation by Pdcd4, this being paralleled by a reduction of Sp1 binding to this region in pdcd4-transfected cells. Pdcd4-transfected cells showed an increase in Sp3 binding to u-PAR promoter region -152/-135, the deletion of which reduces the ability of Pdcd4 to suppress u-PAR promoter activity. Surprisingly, the u-PAR-AP-1 site was not targeted by Pdcd4. Finally, RKO cells overexpressing Pdcd4 showed an inhibition of invasion/intravasation (chicken embryo metastasis assay). These data suggest Pdcd4 as a new negative regula…
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“It was shown that PDCD4 interacts with Sp1 and Sp3 inhibiting the u-PAR expression and tumor invasion/intravasion [39].”
From Control Mechanisms of the Tumor Suppressor PDCD4: Expression and Functions (2019) · cites this paper as [39]
In the citing paper
“The anti-tumor activity of PDCD4 is well documented in invitro systems especially by using colon cancer cell lines [24,39]”
From Control Mechanisms of the Tumor Suppressor PDCD4: Expression and Functions (2019) · cites this paper as [39]
Citations
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Asangani, I. A., Rasheed, S. A. K., Nikolova, D. A., Leupold, J. H., Colburn, N. H., Post, S., & Allgayer, H. (2008) · Oncogene · doi:10.1038/sj.onc.1210856 · Cited by 1,882
Self-citationMicroRNA: Biogenesis, Function and Role in Cancer
MacFarlane, L.-A., & R. Murphy, P. (2010) · Current Genomics · doi:10.2174/138920210793175895 · Cited by 1,584
MicroRNA-21 targets tumor suppressor genes in invasion and metastasis
Zhu, S., Wu, H., Wu, F., Nie, D., Sheng, S., & Mo, Y.-Y. (2008) · Cell Research · doi:10.1038/cr.2008.24 · Cited by 1,051
miR‐21: a small multi‐faceted RNA
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Potential anticancer properties and mechanisms of action of curcumin.
Vallianou, N. G., Evangelopoulos, A. A., Schizas, N., & Kazazis, C. E. (2015) · PubMed · Cited by 333
Metadata
- Authors
- Leupold, J. H., Yang, H.-S., Colburn, N. H., Asangani, I., Post, S., & Allgayer, H.
- Affiliations
- German Cancer Research Center (DE)
- National Institutes of Health (US)
- Heidelberg University (DE)
- Corresponding author
- Allgayer, H
- Venue
- Oncogene
- Published
- 2007-02-12
- Volume
- 26
- Issue
- 31
- Pages
- 4550-4562
- ISSN
- 0950-9232 (ISSN-L)
- 1476-5594
- OpenAlex
- W2038623184
- Cited by
- 163
- References
- 64
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 1 · 2023: 3 · 2022: 5 · 2021: 5 · 2020: 6 · 2019: 6 · 2018: 7 · 2017: 8 · 2016: 13 · 2015: 8 · 2014: 18 · 2013: 15 · 2012: 11
- FWCI
- 4.25
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Oncogene (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Protease and Inhibitor Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mechanisms of cancer metastasis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › TGF-β signaling in diseases
- Keywords
- PDCD4, tumor suppressor, urokinase receptor, Sp1, Sp3, transcriptional regulation, tumor invasion, intravasation, colorectal cancer, metastasis, activator protein-1
- MeSH terms
- Binding Sites, Humans, Neoplasm Invasiveness, Neoplasm Metastasis, Receptors, Cell Surface, Colorectal Neoplasms, Gene Expression Regulation, Neoplastic, Sp1 Transcription Factor, RNA-Binding Proteins, Disease Progression, Tumor Suppressor Proteins, Cell Line, Tumor, Apoptosis Regulatory Proteins, Sp Transcription Factors, Sp3 Transcription Factor, Receptors, Urokinase Plasminogen Activator
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