Optical control of neuronal excitation and inhibition using a single opsin protein, ChR2
Open accessAbstract
The effect of electrical stimulation on neuronal membrane potential is frequency dependent. Low frequency electrical stimulation can evoke action potentials, whereas high frequency stimulation can inhibit action potential transmission. Optical stimulation of channelrhodopsin-2 (ChR2) expressed in neuronal membranes can also excite action potentials. However, it is unknown whether optical stimulation of ChR2-expressing neurons produces a transition from excitation to inhibition with increasing light pulse frequencies. Here we report optical inhibition of motor neuron and muscle activity in vivo in the cooled sciatic nerves of Thy1-ChR2-EYFP mice. We also demonstrate all-optical single-wavelength control of neuronal excitation and inhibition without co-expression of inhibitory and excitatory opsins. This all-optical system is free from stimulation-induced electrical artifacts and thus provides a new approach to investigate mechanisms of high frequency inhibition in neuronal circuits in vivo and in vitro.
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A study with mice proved that both excitation and inhibition can be obtained through using only channelrhodopsin-2.
“A study done with mice proved that both excitation and inhibition can be obtained through using only channelrhodopsin-2 [133].”
From Interfaces with the peripheral nervous system for the control of a neuroprosthetic limb: a review (2020) · cites this paper as [133]
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- Authors
- Liske, H., Qian, X., Anikeeva, P., Deisseroth, K., & Delp, S.
- Affiliations
- Stanford University (US)
- Stanford Medicine (US)
- Massachusetts Institute of Technology (US)
- Corresponding author
- Delp, Scott
- Venue
- Scientific Reports
- Published
- 2013-10-31
- Volume
- 3
- Issue
- 1
- Pages
- 3110-3110
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC3813941
- OpenAlex
- W2148043484
- Cited by
- 42
- References
- 49
- Citations by year
- 2025: 1 · 2024: 3 · 2023: 1 · 2022: 1 · 2020: 7 · 2019: 2 · 2018: 6 · 2017: 4 · 2016: 7 · 2015: 5 · 2014: 5
- FWCI
- 2.78
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DSpace@MIT (Massachusetts Institute of Technology) (submittedVersion, cc-by-nc)
- Funders
- McGill University
- National Institutes of Health
- Stanford Bio-X
- National Institute of General Medical Sciences (T32GM008412)
- National Institute of Neurological Disorders and Stroke (R01NS080954)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Photoreceptor and optogenetics research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neural Engineering
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- channelrhodopsin-2, optogenetics, neuronal excitation, neuronal inhibition, motor neurons, sciatic nerve, action potential propagation
- MeSH terms
- Channelrhodopsins, Animals, Female, Light, Male, Membrane Potentials, Mice, Transgenic, Neurons, Temperature, Mice
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