The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
Digital Detection of Exosomes by Interferometric Imaging
Open accessAbstract
Exosomes, which are membranous nanovesicles, are actively released by cells and have been attributed to roles in cell-cell communication, cancer metastasis, and early disease diagnostics. The small size (30–100 nm) along with low refractive index contrast of exosomes makes direct characterization and phenotypical classification very difficult. In this work we present a method based on Single Particle Interferometric Reflectance Imaging Sensor (SP-IRIS) that allows multiplexed phenotyping and digital counting of various populations of individual exosomes (>50 nm) captured on a microarray-based solid phase chip. We demonstrate these characterization concepts using purified exosomes from a HEK 293 cell culture. As a demonstration of clinical utility, we characterize exosomes directly from human cerebrospinal fluid (hCSF). Our interferometric imaging method could capture, from a very small hCSF volume (20 uL), nanoparticles that have a size compatible with exosomes, using antibodies directed against tetraspanins. With this unprecedented capability, we foresee revolutionary implications in the clinical field with improvements in diagnosis and stratification of patients affected by different disorders.
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Cites
Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids
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B lymphocytes secrete antigen-presenting vesicles.
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Exosome and Exosomal MicroRNA: Trafficking, Sorting, and Function
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Sizing and phenotyping of cellular vesicles using Nanoparticle Tracking Analysis
Dragovic, R. A., Gardiner, C., Brooks, A. S., Tannetta, D. S., Ferguson, D. J. P., Hole, P., Carr, B., Redman, C. W. G., Harris, A. L., Dobson, P. J., et al. (2011) · Nanomedicine: Nanotechnology, Biology and Medicine · doi:10.1016/j.nano.2011.04.003 · Cited by 1,362
Label-free detection and molecular profiling of exosomes with a nano-plasmonic sensor
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Large-scale proteomics and phosphoproteomics of urinary exosomes
Gonzales, P. A., Pisitkun, T., Hoffert, J. D., Tchapyjnikov, D., Star, R. A., Kleta, R., Wang, N. S., & Knepper, M. A. (2009) · Journal of the American Society of Nephrology · doi:10.1681/asn.2008040406 · Cited by 727
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Isolation and Characterization of RNA-Containing Exosomes
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Cited by
Welsh, J. A., Goberdhan, D. C. I., O'Driscoll, L., Buzas, E. I., Blenkiron, C., Bussolati, B., Cai, H., Di Vizio, D., Driedonks, T. A. P., Erdbrügger, U., et al. (2024) · Journal of Extracellular Vesicles · doi:10.1002/jev2.12404 · Cited by 4,663
Pegtel, D. M., & Gould, S. J. (2019) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-013118-111902 · Cited by 2,682
Challenges and directions in studying cell–cell communication by extracellular vesicles
van Niel, G., Carter, D. R. F., Clayton, A., Lambert, D. W., Raposo, G., & Vader, P. (2022) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-022-00460-3 · Cited by 1,229
Erdbrügger, U., Blijdorp, C. J., Bijnsdorp, I. V., Borràs, F. E., Burger, D., Bussolati, B., Byrd, J. B., Clayton, A., Dear, J. W., Falcón‐Pérez, J. M., et al. (2021) · Journal of Extracellular Vesicles · doi:10.1002/jev2.12093 · Cited by 404
Using single-vesicle technologies to unravel the heterogeneity of extracellular vesicles
Bordanaba-Florit, G., Royo, F., Kruglik, S. G., & Falcón-Pérez, J. M. (2021) · Nature Protocols · doi:10.1038/s41596-021-00551-z · Cited by 325
Metadata
- Authors
- Daaboul, G. G., Gagni, P., Benussi, L., Bettotti, P., Ciani, M., Cretich, M., Freedman, D. S., Ghidoni, R., Ozkumur, A. Y., Piotto, C., Prosperi, D., Santini, B., Ünlü, M. S., & Chiari, M.
- Venue
- Scientific Reports
- Published
- 2016-11-17
- Volume
- 6
- Issue
- 1
- Pages
- 37246-37246
- ISSN
- 2045-2322 (ISSN-L)
- OpenAlex
- W2549542099
- Cited by
- 292
- References
- 31
- FWCI
- 6.47
- Type
- journal article
- Language
- en
- Open access
- gold
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- Daaboul, G. G., Gagni, P., Benussi, L., Bettotti, P., Ciani, M., Cretich, M., Freedman, D. S., Ghidoni, R., Ozkumur, A. Y., Piotto, C., Prosperi, D., Santini, B., Ünlü, M. S., & Chiari, M. (2016). Digital Detection of Exosomes by Interferometric Imaging. Scientific Reports. https://doi.org/10.1038/srep37246
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