The DOI matches its Crossref record; 58 of the 58 references it cites were found in a registry or an index.
Human Mesenchymal stem cells program macrophage plasticity by altering their metabolic status via a PGE2-dependent mechanism
Open accessAbstract
Mesenchymal stem cells (MSCs) are speculated to act at macrophage-injury interfaces to mediate efficient repair. To explore this facet in-depth this study evaluates the influence of MSCs on human macrophages existing in distinct functional states. MSCs promoted macrophage differentiation, enhanced respiratory burst and potentiated microbicidal responses in naïve macrophages (Mφ). Functional attenuation of inflammatory M1 macrophages was associated with a concomitant shift towards alternatively activated M2 state in MSC-M1 co-cultures. In contrast, alternate macrophage (M2) activation was enhanced in MSC-M2 co-cultures. Elucidation of key macrophage metabolic programs in Mo/MSC, M1/MSC and M2/MSC co-cultures indicated changes in Glucose transporter1 (GLUT1 expression/glucose uptake, IDO1 protein/activity, SIRTUIN1 and alterations in AMPK and mTOR activity, reflecting MSC-instructed metabolic shifts. Inability of Cox2 knockdown MSCs to attenuate M1 macrophages and their inefficiency in instructing metabolic shifts in polarized macrophages establishes a key role for MSC-secreted PGE 2 in manipulating macrophage metabolic status and plasticity. Functional significance of MSC-mediated macrophage activation shifts was further validated on human endothelial cells prone to M1 mediated injury. In conclusion, we propose a novel role for MSC secreted factors induced at the MSC-macrophage interface in re-educating macrophages by manipulating metabolic programs in differentially polarized macrophages.
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Immunoregulatory mechanisms of mesenchymal stem and stromal cells in inflammatory diseases
Shi, Y., Wang, Y., Li, Q., Liu, K., Hou, J., Shao, C., & Wang, Y. (2018) · Nature Reviews Nephrology · doi:10.1038/s41581-018-0023-5 · Cited by 1,223
Han, Y., Yang, J., Fang, J., Zhou, Y., Candi, E., Wang, J., Hua, D., Shao, C., & Shi, Y. (2022) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-022-00932-0 · Cited by 720
Insights into the Secretome of Mesenchymal Stem Cells and Its Potential Applications
Eleuteri, S., & Fierabracci, A. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20184597 · Cited by 303
Peruzzotti-Jametti, L., Bernstock, J. D., Vicario, N., Costa, A. S. H., Kwok, C. K., Leonardi, T., Booty, L. M., Bicci, I., Balzarotti, B., Volpe, G., et al. (2018) · Cell Stem Cell · doi:10.1016/j.stem.2018.01.020 · Cited by 285
Cao, J., Wang, B., Tang, T., Lv, L., Ding, Z., Li, Z., Hu, R., Wei, Q., Shen, A., Fu, Y., et al. (2020) · Stem Cell Research & Therapy · doi:10.1186/s13287-020-01719-2 · Cited by 264
Metadata
- Authors
- Vasandan, A. B., Jahnavi, S., Shashank, C., Prasad, P., Kumar, A., & Prasanna, S. J.
- Venue
- Scientific Reports
- Published
- 2016-12-02
- Volume
- 6
- Issue
- 1
- Pages
- 38308-38308
- ISSN
- 2045-2322 (ISSN-L)
- OpenAlex
- W2558608698
- Cited by
- 406
- References
- 58
- FWCI
- 16.83
- Type
- journal article
- Language
- en
- Open access
- gold
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- Vasandan, A. B., Jahnavi, S., Shashank, C., Prasad, P., Kumar, A., & Prasanna, S. J. (2016). Human Mesenchymal stem cells program macrophage plasticity by altering their metabolic status via a PGE2-dependent mechanism. Scientific Reports. https://doi.org/10.1038/srep38308
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