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Cited by 74Mangiferin and Mango ExtractsInflammasome and immune disordersBioactive natural compounds

Mangiferin from Pueraria tuberosa reduces inflammation via inactivation of NLRP3 inflammasome

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Bulugonda, Ramakrishna K., kumar, Kotha Anil, Gangappa, D., Beeda, Harshavardhan, Philip, Gundala Harold, Muralidhara Rao, Dowlathabad, Faisal, Syed M.·2017·Scientific Reports
DOI 10.1038/srep42683PMC PMC5316935
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Abstract

Recent reports have demonstrated the role of phyto-constituents in modulating inflammatory responses. Mangiferin isolated from Mangifera indica is known to induce potent anti-oxidative, anti-diabetic and anti-inflammatory activity. However, the molecular mechanism of its anti-inflammatory activity is not properly understood. In this study we have isolated Mangiferin from the tubers of Pueraria tuberosa (PT-Mangiferin) and analysed the mechanism of its potent anti-inflammatory effects in LPS stimulated RAW 264.7 mouse macrophage cell line and in a carrageenan induced air pouch model. PT-Mangiferin was non-toxic to primary cells but showed significant toxicity and apoptotic effect on cancerous cells. It significantly reduced the production of pro-inflammatory mediators (COX-2, iNOS and TNF-α) in LPS stimulated RAW 264.7 cells. Further, it has also reduced the generation of ROS and inhibited LPS induced NF-kB translocation in these cells. Additionally, PT-Mangiferin significantly reduced inflammation in a mouse air pouch model by inhibiting the infiltration of monocytes and neutrophils and reducing the production of cytokines. These effects were mediated via inactivation of NLRP3 inflammasome complex and its downstream signalling molecules. Taken together these results suggest that PT-Mangiferin is potent anti-inflammatory compound that reduces inflammation and holds promise in development of herbal based anti-inflammatory therapeutics in future.

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  • In vitro studies in macrophage cell lines showed that MGF treatment decreased proinflammatory factors and ROS promoted by LPS stress.

    “in vitro studies, suggesting that MGF treatment produced in decrease in proinflammatory factors and ROS promoted by LPS stress in different macrophage cell lines [42,43].”

    From Effects of Mangiferin on LPS-Induced Inflammation and SARS-CoV-2 Viral Adsorption in Human Lung Cells (2022) · cites this paper as [43]

    The Results report that PT-mangiferin reduced LPS-induced ROS and inflammatory mediators in RAW 264.7 macrophages. The Cell culture and ROS methods identify only that macrophage cell line for these experiments, so this source does not establish the claim's reference to different macrophage cell lines. Page numbers were not provided in our copy. (LLM, conf=0.85)

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Authors
Bulugonda, R. K., kumar, K. A., Gangappa, D., Beeda, H., Philip, G. H., Muralidhara Rao, D., & Faisal, S. M.
Affiliations
  • Sri Krishnadevaraya University (IN)
  • University of Hyderabad (IN)
  • National Institute of Animal Biotechnology (IN)
Corresponding author
Faisal, Syed M.
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2017-02-20
Volume
7
Issue
1
Pages
42683-42683
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/srep42683
PMC
PMC5316935
OpenAlex
W2591036296
Cited by
74
References
9
Citations by year
2026: 5 · 2025: 4 · 2024: 10 · 2023: 12 · 2022: 8 · 2021: 9 · 2020: 14 · 2019: 5 · 2018: 5 · 2017: 2
FWCI
10.54
Type
journal article
Language
en
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
  • Department of Science and Technology, Ministry of Science and Technology, India
  • Indian Council of Medical Research
  • Science and Engineering Research Board
Topics
  • Health Sciences › Medicine › Complementary and alternative medicine › Mangiferin and Mango Extracts
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Inflammasome and immune disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bioactive natural compounds
Keywords
mangiferin, Pueraria tuberosa, NLRP3 inflammasome, anti-inflammatory activity, macrophages, lipopolysaccharide-induced inflammation, reactive oxygen species, cyclooxygenase-2, inducible nitric oxide synthase, TNF-α, air pouch model
MeSH terms
NLR Family, Pyrin Domain-Containing 3 Protein, Animals, Anti-Inflammatory Agents, Cell Line, Humans, Lipopolysaccharides, Macrophages, Male, Plant Extracts, Signal Transduction, NF-kappa B, Apoptosis, Reactive Oxygen Species, Inflammation Mediators, Protein Transport, Pueraria, Cell Proliferation, Mice, Inflammasomes

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