Micro-bioreactor array for controlling cellular microenvironments
Open accessAbstract
High throughput experiments can be used to spatially and temporally investigate the many factors that regulate cell differentiation. We have developed a micro-bioreactor array (MBA) that is fabricated using soft lithography and contains twelve independent micro-bioreactors perfused with culture medium. The MBA enables cultivation of cells that are either attached to substrates or encapsulated in hydrogels, at variable levels of hydrodynamic shear, and with automated image analysis of the expression of cell differentiation markers. The flow and mass transport in the MBA were characterized by computational fluid dynamic (CFD) modeling. The representative MBA configurations were validated using the C2C12 cell line, primary rat cardiac myocytes and human embryonic stem cells (hESCs) (lines H09 and H13). To illustrate the utility of the MBA for controlled studies of hESCs, we established correlations between the expression of smooth muscle actin and cell density for three different flow configurations.
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Novel 3D cell culture platforms are being created to better mimic in vivo conditions and are sometimes called spheroid or organoid culture.
“To overcome this limitation, novel 3D cell culture platforms are being created to better mimic in vivo conditions and are sometimes called spheroid or organoid culture, as described below (37,61,84,98,117,118).”
From Modeling physiological events in 2D vs. 3D cell culture (2017) · cites this paper as [37]
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- Authors
- Figallo, E., Cannizzaro, C., Gerecht, S., Burdick, J. A., Langer, R., Elvassore, N., & Vunjak-Novakovic, G.
- Affiliations
- University of Padua (IT)
- Massachusetts Institute of Technology (US)
- University of Pennsylvania (US)
- Corresponding author
- Elvassore, Nicola
- Venue
- Lab on a Chip
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2007
- Volume
- 7
- Issue
- 6
- Pages
- 710
- ISSN
- 1473-0189 (ISSN-L)
- 1473-0197
- OpenAlex
- W1982220375
- Cited by
- 220
- References
- 50
- Citations by year
- 2025: 2 · 2024: 4 · 2023: 6 · 2022: 6 · 2021: 7 · 2020: 9 · 2019: 3 · 2018: 11 · 2017: 8 · 2016: 7 · 2015: 15 · 2014: 9 · 2013: 19 · 2012: 18
- FWCI
- 17.45
- Type
- journal article
- Language
- en
- Open access
- green
- doi.org
- Locations
- Lab on a Chip (publishedVersion)
- PubMed (publishedVersion)
- Padua Research Archive (University of Padova) (submittedVersion)
- Columbia Academic Commons (Columbia University)
- Funders
- National Institutes of Health (2r01hl076485-16)
- National Heart, Lung, and Blood Institute (R01-HL076485)
- National Institute of Biomedical Imaging and Bioengineering (P41EB002520)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Keywords
- cell microenvironment, cell differentiation, hydrogel encapsulation, hydrodynamic shear, C2C12 myoblasts, cardiomyocytes, human embryonic stem cells, smooth muscle actin, cell density, computational fluid dynamics, high-throughput cell culture, soft lithography
- MeSH terms
- Actins, Animals, Cell Count, Cell Differentiation, Computer Simulation, Culture Media, Humans, Models, Biological, Muscle, Smooth, Rheology, Cell Culture Techniques, Bioreactors, Tissue Engineering, Myocytes, Cardiac, Shear Strength, Nanotechnology, Rats, Embryonic Stem Cells
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