Photoimmune protective effect of the phytoestrogenic isoflavonoid equol is partially due to its antioxidant activities
Abstract
Topical application of lotions containing the phytoestrogenic isoflavonoid equol have been reported to protect mice against UV radiation-induced inflammation, immune suppression and photocarcinogenesis. The photoimmune protective property was shown to depend on equol's activation of oestrogen receptor signalling in the skin. However, isoflavones are also recognised for their antioxidant properties in biological systems. As endogenous cutaneous antioxidant enzymes including the inducible stress protein haem oxygenase (HO)-1, have photoprotective efficacy, this study in the Skh:hr-1 hairless mouse seeks evidence for an antioxidant role for equol in contributing to its photoimmune protection. Oxidative stress has been measured as UVA-induced lipid peroxidation in the mouse skin, and was dose-dependently inhibited by topical equol. Inhibition of the UVA (320-400 nm)-inducible HO activity significantly reduced the level of equol protection against lipid peroxidation, thereby attributing a component of equol's lipid protection capacity to this stress enzyme. It was consistent that topical equol enhanced the level of HO induction by UVA irradiation in both skin and liver. Subsequently, the dose-dependent protection by topical equol lotions against solar simulated UV radiation induced immunosuppression, measured by the contact hypersensitivity reaction, was found also to be partially reduced by the inhibition of HO activity. Therefore, in addition to the activation by equol of oestrogenic signalling pathways for photoprotection, this isoflavonoid also provides UV-protective antioxidant effects that depend partially on HO-1 induction.
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Equol has UV-protective antioxidant effects via the endogenous cutaneous antioxidant enzyme haem oxygenase (HO)-1.
“other investigators have reported the positive biological activities of equol that include the UV-protective antioxidant effects via the endogenous cutaneous antioxidant enzyme haem oxygenase (HO)-1 [94].”
Equol stimulates gene expression of SH-rich detoxifying proteins metallothionein-1H and metallothionein-2H that protect against metal toxicity.
“equol has been shown to stimulate the gene expression of the SH-rich detoxifying proteins, like metallothionein-1H and metallothionein-2H, that protect against metal toxicity [85,94].”
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- Authors
- Widyarini, S., Domanski, D., Painter, N., & Reeve, V. E.
- Affiliations
- The University of Sydney (AU)
- Corresponding author
- Reeve, Vivienne E.
- ORCID
- Widyarini, Sitarina
- Venue
- Photochemical & Photobiological Sciences
- Published
- 2012-07
- Volume
- 11
- Issue
- 7
- Pages
- 1186-1192
- ISSN
- 1474-905X (ISSN-L)
- 1474-9092
- OpenAlex
- W2074199612
- Cited by
- 16
- References
- 31
- Citations by year
- 2024: 2 · 2023: 1 · 2021: 2 · 2018: 1 · 2017: 1 · 2016: 2 · 2015: 1 · 2014: 4 · 2012: 2
- FWCI
- 2.80
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Photochemical & Photobiological Sciences (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Health Sciences › Medicine › Pathology and Forensic Medicine › Phytoestrogen effects and research
- Keywords
- equol, isoflavonoids, phytoestrogens, antioxidant activity, heme oxygenase-1, oxidative stress, UV-induced immunosuppression, contact hypersensitivity, topical application
- MeSH terms
- Animals, Antioxidants, Dermatitis, Contact, Female, Immunosuppression Therapy, Isoflavones, Liver, Mice, Hairless, Skin, Ultraviolet Rays, Lipid Peroxidation, Oxidative Stress, Phytoestrogens, Mice, Heme Oxygenase-1, Equol
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