3D microvascular model recapitulates the diffuse large B-cell lymphoma tumor microenvironment in vitro
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This 3D multiplex in vitro microvascular model of the DLBCL microenvironment incorporates an endothelialized microvessel traversing a hydrogel-based tumor model.
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Today, tumor-on-chip systems are the most promising embodiment of in vitro platforms capable of faithfully recapitulating relevant aspects of the biochemical complexity and dynamics of the tumor niche in a controlled environment with much lower variability than in vivo systems.
“Today, tumor-on-chip systems are the most promising embodiment of in vitro platforms capable of faithfully recapitulation of relevant aspects of the biochemical complexity and dynamics of the tumor niche in a controlled environment (i.e., with much lower variability than is associated with in vivo systems [8,54,113]).”
From The Tumor-on-Chip: Recent Advances in the Development of Microfluidic Systems to Recapitulate the Physiology of Solid Tumors (2019) · cites this paper as [54]
The Introduction says in vitro models can recapitulate aspects of in vivo systems while mitigating biological variability, and the Discussion says this lymphoma-on-chip model captures complex tumor-cell interactions and spatiotemporal drug delivery. The paper does not establish that tumor-on-chip systems generally are the “most promising” in vitro platforms; it also identifies limitations in earlier tumor-on-chip models. Page numbers are not provided in our copy. (LLM, conf=0.85)
Experimental — accuracy not yet measuredMannino et al. demonstrated a simple strategy that enables researchers to recreate tumor microenvironments using resources readily available in most labs.
“Overall, Mannino et al. demonstrated a simple strategy that enables researchers to recreate tumor microenvironments using resources readily available in most labs [54].”
From The Tumor-on-Chip: Recent Advances in the Development of Microfluidic Systems to Recapitulate the Physiology of Solid Tumors (2019) · cites this paper as [54]
The system recapitulated the interactions between cancer, endothelial, and immune cells.
“The system recapitulated the interactions between cancer, endothelial, and immune cells.”
From The Tumor-on-Chip: Recent Advances in the Development of Microfluidic Systems to Recapitulate the Physiology of Solid Tumors (2019) · cites this paper as [54]
This simple tumor-on-chip was adequate for testing the efficacy of an immunotherapy drug.
“Notably, this simple tumor-on-chip was adequate for testing the efficacy of an immunotherapy drug.”
From The Tumor-on-Chip: Recent Advances in the Development of Microfluidic Systems to Recapitulate the Physiology of Solid Tumors (2019) · cites this paper as [54]
Mannino et al. fabricated a continuously perfusable system with a central vessel of approximately 350 μm to recreate an in vitro B-cell lymphoma tumor model.
“For example, Mannino et al. [54] fabricated a continuously perfusable system with a central vessel of approximately 350 µm to recreate an in vitro B-cell lymphoma tumor model (Figure 7a).”
From The Tumor-on-Chip: Recent Advances in the Development of Microfluidic Systems to Recapitulate the Physiology of Solid Tumors (2019) · cites this paper as [54]
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“In another study [54], A20 cell line mouse B-cell lymphoma was used to replicate diffuse large B-cell lymphoma (DLBCL), and mouse lung microvascular endothelial cells (MLMVECs) were used as endothelial cells.”
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- Authors
- Mannino, R. G., Santiago-Miranda, A. N., Pradhan, P., Qiu, Y., Mejias, J. C., Neelapu, S. S., Roy, K., & Lam, W. A.
- Affiliations
- Georgia Institute of Technology (US)
- The Wallace H. Coulter Department of Biomedical Engineering at Georgia Tech and Emory University
- The University of Texas MD Anderson Cancer Center (US)
- Corresponding author
- Lam, Wilbur A.
- ORCID
- Venue
- Lab on a Chip
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2017
- Volume
- 17
- Issue
- 3
- Pages
- 407-414
- ISSN
- 1473-0189 (ISSN-L)
- 1473-0197
- PMC
- PMC5285444
- OpenAlex
- W2562160912
- Cited by
- 89
- References
- 31
- Citations by year
- 2026: 5 · 2025: 11 · 2024: 8 · 2023: 11 · 2022: 13 · 2021: 12 · 2020: 8 · 2019: 8 · 2018: 9 · 2017: 4
- FWCI
- 3.37
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Lab on a Chip (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- American Heart Association (Innovative Research Grant)
- National Science Foundation (CAREER Award, GRFP)
- National Institutes of Health (5U01-HL117721, R01HL121264, U54HL112309)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Keywords
- diffuse large B-cell lymphoma, tumor microenvironment, 3D tumor models, microfabrication, endothelial cells, drug delivery
- MeSH terms
- Animals, Mice, Inbred BALB C, Models, Biological, Tumor Cells, Cultured, Lymphoma, Large B-Cell, Diffuse, Microfluidic Analytical Techniques, Mice, Tumor Microenvironment
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