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Simultaneous identification of species and molecular forms of the Anopheles gambiae complex by PCR‐RFLP
Abstract
Abstract For differential identification of sibling species in the Anopheles gambiae Giles complex (Diptera: Culicidae), including simultaneous separation of M and S molecular forms within An. gambiae Giles sensu stricto , we describe a PCR‐RFLP method. This procedure is more efficient, faster and cheaper than those used before, so is recommended for large‐scale processing of field‐collected larval and adult specimens to be identified in malaria vector studies.
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“Anopheles gambiae s.s. and Anopheles coluzzii were further identified using PCR-restriction fragment length polymorphism (RFLP) [29].”
Spatial temporal distribution of Anopheles mosquitoes in different ecological zones of Ghana
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“Anopheles gambiae s.s and An. coluzzii were further distinguished by PCR-RFLP using the method by Fanello et al. [26].”
Metadata
- Authors
- Fanello, C., Santolamazza, F., & Della Torre, A.
- Venue
- Medical and Veterinary Entomology
- Publisher
- Wiley
- Published
- 2002-12
- Volume
- 16
- Issue
- 4
- Pages
- 461-464
- ISSN
- 0269-283X (ISSN-L)
- 1365-2915
- OpenAlex
- W2015937705
- Cited by
- 517
- References
- 6
- FWCI
- 5.00
- Type
- journal article
- Language
- en
- Open access
- closed
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