The transcriptional response to alkaline pH in Saccharomyces cerevisiae: evidence for calcium‐mediated signalling

Serrano, Raquel; Ruiz, Amparo; Bernal, Dolores; Chambers, James R.; Ariño, Joaquín

doi:10.1046/j.1365-2958.2002.03246.x

Cited by 198Fungal and yeast genetics researchSignaling Pathways in DiseaseBacterial Genetics and Biotechnology

The transcriptional response to alkaline pH in Saccharomyces cerevisiae: evidence for calcium‐mediated signalling

Serrano, Raquel, Ruiz, Amparo, Bernal, Dolores, Chambers, James R., Ariño, Joaquín·2002·Molecular Microbiology

DOI 10.1046/j.1365-2958.2002.03246.x

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Abstract

The short-time transcriptional response of yeast cells to a mild increase in external pH (7.6) has been investigated using DNA microarrays. A total of 150 genes increased their mRNA level at least twofold within 45 min. Alkalinization resulted in the repression of 232 genes. The response of four upregulated genes, ENA1 (encoding a Na+-ATPase also induced by saline stress) and PHO84, PHO89 and PHO12 (encoding genes upregulated by phosphate starvation), was characterized further. The alkaline response of ENA1 was not affected by mutation of relevant genes involved in osmotic or oxidative signalling, but was decreased in calcineurin and rim101 mutants. Mapping of the ENA1 promoter revealed two pH-responsive regions. The response of the upstream region was fully abolished by the drug FK506 or mutation of CRZ1 (a transcription factor activated by calcium/calcineurin), whereas the response of the downstream region was essentially calcium independent. PHO84 and PHO12 responses were unaffected in crz1 cells, but required the presence of Pho2 and Pho4. In contrast, part of the alkali-induced expression of PHO89 was maintained in pho4 or pho2 cells, but was fully abolished in a crz1 strain or in the presence of FK506. Heterologous promoters carrying the minimal calcineurin-dependent response elements found in ENA1 or FKS2 were able to drive alkaline pH-induced expression. These results demonstrate that the transcriptional response to alkaline pH involves different signalling mechanisms, and that calcium signalling is a relevant component of this response.

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Calcineurin-mediated dephosphorylation of Crz1 and its entry into the nucleus induces expression of several alkaline pH responsive genes.
“Subsequently, calcineurin-mediated dephosphorylation of the Crz1 transcription factor and its entry into the nucleus induces the expression of several alkaline pH responsive genes (Serrano et al., 2002; Stathopoulos-Gerontides et al., 1999).”
From Calcineurin as a multifunctional regulator: Unraveling novel functions in fungal stress responses, hyphal growth, drug resistance, and pathogenesis (2014) · cites this paper as Serrano et al., 2002

Current protocols in molecular biology
Dudley, R. K. (1988) · FEBS Letters · doi:10.1016/0014-5793(88)80264-3 · Cited by 6,037 · see citing works
Methods in Yeast Genetics
Jacob, H. (1988) · Bioelectrochemistry and Bioenergetics · doi:10.1016/0302-4598(88)80052-7 · Cited by 4,911 · see citing works
Genomic Expression Programs in the Response of Yeast Cells to Environmental Changes
Gasch, A. P., Spellman, P. T., Kao, C. M., Carmel-Harel, O., Eisen, M. B., Storz, G., Botstein, D., & Brown, P. O. (2000) · Molecular Biology of the Cell · doi:10.1091/mbc.11.12.4241 · Cited by 4,815 · see citing works
Functional Characterization of the S. cerevisiae Genome by Gene Deletion and Parallel Analysis
Winzeler, E. A., Shoemaker, D. D., Astromoff, A., Liang, H., Anderson, K., Andre, B., Bangham, R., Benito, R., Boeke, J. D., Bussey, H., et al. (1999) · Science · doi:10.1126/science.285.5429.901 · Cited by 3,953 · see citing works
New heterologous modules for classical or PCR‐based gene disruptions in Saccharomyces cerevisiae
Wach, A., Brachat, A., Pöhlmann, R., & Philippsen, P. (1994) · Yeast · doi:10.1002/yea.320101310 · Cited by 2,532 · see citing works
Remodeling of Yeast Genome Expression in Response to Environmental Changes
Causton, H. C., Ren, B., Koh, S. S., Harbison, C. T., Kanin, E. I., Jennings, E. G., Lee, T. I., True, H. L., Lander, E. S., & Young, R. A. (2001) · Molecular Biology of the Cell · doi:10.1091/mbc.12.2.323 · Cited by 1,364 · see citing works
MIPS: a database for genomes and protein sequences
Mewes, H. (2000) · Nucleic Acids Research · doi:10.1093/nar/28.1.37 · Cited by 1,276 · see citing works
Heme regulates transcription of the CYC1 gene of S. cerevisiae via an upstream activation site
Guarente, L., & Mason, T. L. (1983) · Cell · doi:10.1016/0092-8674(83)90309-4 · Cited by 644 · see citing works
Stress-controlled transcription factors, stress-induced genes and stress tolerance in budding yeast
Estruch, F. (2000) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.2000.tb00551.x · Cited by 616 · see citing works
Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions
Myers, A. M., Tzagoloff, A., Kinney, D. M., & Lusty, C. J. (1986) · Gene · doi:10.1016/0378-1119(86)90028-4 · Cited by 613 · see citing works
The Transcriptional Response of Saccharomyces cerevisiae to Osmotic Shock
Rep, M., Krantz, M., Thevelein, J. M., & Hohmann, S. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.275.12.8290 · Cited by 554 · see citing works
New Components of a System for Phosphate Accumulation and Polyphosphate Metabolism inSaccharomyces cerevisiaeRevealed by Genomic Expression Analysis
Ogawa, N., DeRisi, J. L., & Brown, P. O. (2000) · Molecular Biology of the Cell · doi:10.1091/mbc.11.12.4309 · Cited by 494 · see citing works
Calcineurin acts through theCRZ1/TCN1-encoded transcription factor to regulate gene expression in yeast
Stathopoulos, A. M., & Cyert, M. S. (1997) · Genes & Development · doi:10.1101/gad.11.24.3432 · Cited by 442 · see citing works
Calcineurin Inhibits VCX 1-Dependent H + /Ca 2 + Exchange and Induces Ca 2 + ATPases in Saccharomyces cerevisiae
Cunningham, K. W., & Fink, G. R. (1996) · Molecular and Cellular Biology · doi:10.1128/mcb.16.5.2226 · Cited by 431 · see citing works
The Transcriptional Response of Yeast to Saline Stress
Posas, F., Chambers, J. R., Heyman, J. A., Hoeffler, J. P., de Nadal, E., & Ariño, J. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m910016199 · Cited by 423 · see citing works
Self-citation
Genome-wide Analysis of Gene Expression Regulated by the Calcineurin/Crz1p Signaling Pathway in Saccharomyces cerevisiae
Yoshimoto, H., Saltsman, K., Gasch, A. P., Li, H. X., Ogawa, N., Botstein, D., Brown, P. O., & Cyert, M. S. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m202718200 · Cited by 404 · see citing works
The plasma membrane of Saccharomyces cerevisiae: structure, function, and biogenesis
van der Rest, M. E., Kamminga, A. H., Nakano, A., Anraku, Y., Poolman, B., & Konings, W. N. (1995) · Microbiological Reviews · doi:10.1128/mr.59.2.304-322.1995 · Cited by 359 · see citing works
Salt Tolerance in Plants and Microorganisms: Toxicity Targets and Defense Responses
Serrano, R. (1996) · International review of cytology · doi:10.1016/s0074-7696(08)62219-6 · Cited by 353 · see citing works
Self-citation
The protein phosphatase calcineurin is essential for NaCl tolerance of Saccharomyces cerevisiae.
Mendoza, I., Rubio, F., Rodriguez-Navarro, A., & Pardo, J. M. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)37038-2 · Cited by 350 · see citing works
Tcn1p/Crz1p, a calcineurin-dependent transcription factor that differentially regulates gene expression in Saccharomyces cerevisiae
Matheos, D. P., Kingsbury, T. J., Ahsan, U. S., & Cunningham, K. W. (1997) · Genes & Development · doi:10.1101/gad.11.24.3445 · Cited by 332 · see citing works
RIM101-Dependent and -Independent Pathways Govern pH Responses in Candida albicans
Davis, D. A., Wilson, R. B., & Mitchell, A. P. (2000) · Molecular and Cellular Biology · doi:10.1128/mcb.20.3.971-978.2000 · Cited by 285 · see citing works
Protein phosphatase type 2B (calcineurin)‐mediated, FK506‐sensitive regulation of intracellular ions in yeast is an important determinant for adaptation to high salt stress conditions.
Nakamura, T., Liu, Y., Hirata, D., Namba, H., Harada, S., Hirokawa, T., & Miyakawa, T. (1993) · The EMBO Journal · doi:10.1002/j.1460-2075.1993.tb06090.x · Cited by 270 · see citing works
Alkaline Response Genes of Saccharomyces cerevisiaeand Their Relationship to the RIM101 Pathway
Lamb, T. M., Xu, W., Diamond, A. E., & Mitchell, A. P. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m008381200 · Cited by 244 · see citing works
Iron and copper transport in yeast and its relevance to human disease
Askwith, C. C., & Kaplan, J. (1998) · Trends in Biochemical Sciences · doi:10.1016/s0968-0004(98)01192-x · Cited by 220 · see citing works
Proteolytic Activation of Rim1p, a Positive Regulator of Yeast Sporulation and Invasive Growth
Li, W., & Mitchell, A. P. (1997) · Genetics · doi:10.1093/genetics/145.1.63 · Cited by 209 · see citing works
Differential expression of two genes encoding isoforms of the ATPase involved in sodium efflux in Saccharomyces cerevisiae
Garcíadeblas, B., Rubio, F., Quintero, F. J., Bañuelos, M. A., Haro, R., & Rodríguez‐Navarro, A. (1993) · Molecular and General Genetics MGG · doi:10.1007/bf00277134 · Cited by 203 · see citing works
Repressors and Upstream Repressing Sequences of the Stress-Regulated ENA1 Gene in Saccharomyces cerevisiae: bZIP Protein Sko1p Confers HOG-Dependent Osmotic Regulation
Proft, M., & Serrano, R. (1999) · Molecular and Cellular Biology · doi:10.1128/mcb.19.1.537 · Cited by 180 · see citing works
Self-citation
Effect of Environmental pH on Morphological Development of Candida albicans Is Mediated via the PacC-Related Transcription Factor Encoded by PRR2
Ramón, A., Porta, A., & Fonzi, W. A. (1999) · Journal of Bacteriology · doi:10.1128/jb.181.24.7524-7530.1999 · Cited by 174 · see citing works
pH Regulation of Gene Expression in Fungi
Denison, S. H. (2000) · Fungal Genetics and Biology · doi:10.1006/fgbi.2000.1188 · Cited by 168 · see citing works
MIPS: a database for genomes and protein sequences
Mewes, H., Heumann, K., Kaps, A., et al. (1999) · Nucleic Acids Research · doi:10.1093/nar/27.1.44 · Cited by 166 · see citing works
MIPS: a database for protein sequences and complete genomes
Mewes, H. (1998) · Nucleic Acids Research · doi:10.1093/nar/26.1.33 · Cited by 135 · see citing works
Multiple transduction pathways regulate the sodium‐extrusion gene PMR2/ENA1 during salt stress in yeast
Márquez, J. A., & Serrano, R. (1996) · FEBS Letters · doi:10.1016/0014-5793(96)00157-3 · Cited by 134 · see citing works
Self-citation
Coordinate genetic control of yeast fatty acid synthase genes FAS1 and FAS2 by an upstream activation site common to genes involved in membrane lipid biosynthesis.
Schüller, H., Hahn, A., Tröster, F., et al. (1992) · The EMBO Journal · doi:10.1002/j.1460-2075.1992.tb05033.x · Cited by 130 · see citing works
Heat Shock-Mediated Cell Cycle Blockage and G1 Cyclin Expression in the Yeast Saccharomyces cerevisiae
Rowley, A., Johnston, G. C., Butler, B. L., et al. (1993) · Molecular and Cellular Biology · doi:10.1128/mcb.13.2.1034-1041.1993 · Cited by 119 · see citing works
Glucose repression affects ion homeostasis in yeast through the regulation of the stress‐activated ENA1 gene
Alepuz, P. M., Cunningham, K. W., & Estruch, F. (1997) · Molecular Microbiology · doi:10.1046/j.1365-2958.1997.5531917.x · Cited by 111 · see citing works
The yeast halotolerance determinant Hal3p is an inhibitory subunit of the Ppz1p Ser/Thr protein phosphatase
de Nadal, E., Clotet, J., Posas, F., Serrano, R., Gómez, N., & Ariño, J. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.13.7357 · Cited by 107 · see citing works
Self-citation
Osmotic stress causes a G1 cell cycle delay and downregulation of Cln3/Cdc28 activity in Saccharomyces cerevisiae
Bellı́, G., Garí, E., Aldea, M., et al. (2001) · Molecular Microbiology · doi:10.1046/j.1365-2958.2001.02297.x · Cited by 90 · see citing works
A Novel Family of Yeast Chaperons Involved in the Distribution of V-ATPase and Other Membrane Proteins
Cohen, A., Perzov, N., Nelson, H. R., & Nelson, N. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.38.26885 · Cited by 90 · see citing works
Phosphate permeases of Saccharomyces cerevisiae: structure, function and regulation
Persson, B. L., Petersson, J., Fristedt, U., et al. (1999) · Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes · doi:10.1016/s0304-4157(99)00010-6 · Cited by 88 · see citing works
Heat shock-mediated cell cycle blockage and G1 cyclin expression in the yeast Saccharomyces cerevisiae.
Rowley, A., Johnston, G. C., Butler, B., et al. (1993) · Molecular and Cellular Biology · doi:10.1128/mcb.13.2.1034 · Cited by 88 · see citing works
Genetic Analysis of Regulatory Mutants Affecting Synthesis of Extracellular Proteinases in the Yeast Yarrowia lipolytica: Identification of a RIM101/pacC Homolog
Lambert, M., Blanchin-Roland, S., Louedec, F. L., Lépingle, A., & Gaillardin, C. M. (1997) · Molecular and Cellular Biology · doi:10.1128/mcb.17.7.3966 · Cited by 85 · see citing works
Yeast Vectors and Assays for Expression of Cloned Genes
Reynolds, A. E., Lundblad, V., Dorris, D. R., & Keaveney, M. (1997) · Current Protocols in Molecular Biology · doi:10.1002/0471142727.mb1306s39 · Cited by 85 · see citing works
Putative membrane components of signal transduction pathways for ambient pH regulation in Aspergillus and meiosis in Saccharomyces are homologous
Denison, S. H., Negrete‐Urtasun, S., Mingot, J. M., et al. (1998) · Molecular Microbiology · doi:10.1046/j.1365-2958.1998.01058.x · Cited by 66 · see citing works
Promoter sequences regulated by the calcineurin-activated transcription factor Crz1 in the yeast ENA1 gene
Mendizabal, I., Pascual-Ahuir, A., Serrano, R., & de Larrinoa, I. (2001) · Molecular Genetics and Genomics · doi:10.1007/s004380100474 · Cited by 57 · cited 3× · see citing works
“This sequence contains the downstream, high-affinity Crz1-binding element identified by Mendizabal et al. (2001).”
BackgroundMethodSelf-citation
Transcriptional Remodeling and G1 Arrest in Dioxygen Stress in Saccharomyces cerevisiae
Lee, J., Romeo, A. M., & Kosman, D. J. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.40.24885 · Cited by 54 · see citing works
Cat8 and Sip4 mediate regulated transcriptional activation of the yeast malate dehydrogenase geneMDH2 by three carbon source-responsive promoter elements
Roth, S., & Sch�ller, H.-J. (2001) · Yeast · doi:10.1002/1097-0061(20010130)18:2 · Cited by 25 · see citing works
Structural and functional heterogeneity of rap1p complexes with telomeric and UASrpg-like DNA sequences
Idrissi, F.-Z., Fernández-Larrea, J., & Piña, B. (1998) · Journal of Molecular Biology · doi:10.1006/jmbi.1998.2215 · Cited by 25 · see citing works
SHC1,a High pH Inducible Gene Required for Growth at Alkaline pH inSaccharomyces cerevisiae
Hong, S. K., Han, S. B., Snyder, M., et al. (1999) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1999.0158 · Cited by 13 · see citing works

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Authors: Serrano, R., Ruiz, A., Bernal, D., Chambers, J. R., & Ariño, J.
Affiliations: Universitat Autònoma de Barcelona (ES)
ORCID: Ruiz, Amparo
Bernal, Dolores
Venue: Molecular Microbiology
Publisher: Wiley
Published: 2002-12
Volume: 46
Issue: 5
Pages: 1319-1333
ISSN: 0950-382X (ISSN-L)
1365-2958
DOI: 10.1046/j.1365-2958.2002.03246.x
OpenAlex: W2170340101
Cited by: 198
References: 48
Citations by year: 2026: 4 · 2025: 5 · 2024: 5 · 2023: 8 · 2022: 6 · 2021: 2 · 2020: 9 · 2019: 4 · 2018: 3 · 2017: 12 · 2016: 10 · 2015: 16 · 2014: 9 · 2013: 8 · 2012: 7
FWCI: 2.78
Type: journal article
Language: en
Open access: closed
Licence: onlinelibrary.wiley.com/termsAndConditions#vor (vor, 8 d)
Locations: Molecular Microbiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Signaling Pathways in Disease
Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords: Saccharomyces cerevisiae, transcriptional regulation, calcium signaling, calcineurin, Crz1, ENA1, Na+-ATPase, PHO84, PHO2, Pho4, FKS2, promoter mapping, gene repression
MeSH terms: Base Sequence, Hydrogen-Ion Concentration, Molecular Sequence Data, Saccharomyces cerevisiae, Transcription, Genetic, Gene Expression Regulation, Fungal, Genome, Fungal, Heat-Shock Response, Calcium Signaling, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, Saccharomyces cerevisiae Proteins

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The transcriptional response to alkaline pH in Saccharomyces cerevisiae: evidence for calcium‐mediated signalling

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