CoChat
Sign Up
Cited by 221Microbial Community Ecology and PhysiologyMethane Hydrates and Related PhenomenaGeochemistry and Elemental Analysis

Bacterial diversity in hydrothermal sediment and epsilonproteobacterial dominance in experimental microcolonizers at the Mid-Atlantic Ridge.

Open access
López‐García, Purificación, Duperron, Sébastien, Philippot, Pascal, Foriel, Julien, Susini, Jean, Moreira, David·2003·Environmental Microbiology
DOI 10.1046/j.1462-2920.2003.00495.x
Open this paper in CoChat — free accountOpen PDF

Abstract

We report here a molecular survey based on 16S rRNA genes of the bacterial diversity found in two deep-sea vent niches at the Mid-Atlantic Ridge: hydrothermal sediment (Rainbow site), and microcolonizers made of three different substrates (organic-rich, iron-rich and pumice) that were exposed for 15 days to a vent emission. Bacterial diversity in sediment samples was scattered through many bacterial divisions. The most abundant and diverse environmental sequences (phylotypes) in our libraries corresponded to the Gammaproteobacteria, followed by the Acidobacteria. We detected members of all the subdivisions within the Proteobacteria. Myxobacterial lineages were the most represented within the delta subdivision. Phylotypes ascribing to the Cytophaga-Flavobacterium-Bacteroides, Planctomycetales, high and low G + C Gram-positives, Nitrospirae, and the candidate division TM7 were also identified. Compared to this broad taxonomic coverage, microcolonizers were almost exclusively colonized by epsilonproteobacteria, although these exhibited considerable morphological and phylogenetic in-group diversity. No specificity for any of the substrates tested was seen. This observation further supports the idea of the ecological dominance of epsilonproteobacteria in the fluid-seawater interface environment. Because oxidation of reduced S species and/or sulphur-reduction is thought to be essential for their energetic metabolism in these areas, we mapped different oxidation states of S in individual bacterial filaments from the iron-rich microcolonizer. For this, we used high-resolution, non-destructive synchrotron micro-X-ray Absorption Near-Edge Spectroscopy (micro-XANES), which revealed the co-existence of different S oxidation states, from sulphide to sulphate, at the level of individual cells. This suggests that these cells were metabolizing sulphur in situ.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1046/j.1462-2920.2003.00495.x

Cites

67 of 67 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • High abundance of Epsilonbacteraeota on newly available surfaces (freshly laid eggs and recently molted pleopods) is in good agreement with previous studies reporting Epsilonbacteraeota as pioneer colonizers on several types of substratum available at vents after just a few days of colonization.

    “High abundance of this group on newly available surfaces, i.e., freshly laid eggs and recently molted pleopods, is in good agreement with previous studies reporting Epsilonbacteraeota as pioneer colonizers on several types of substratum available at vents after just a few days of colonization (López-García et al., 2003; Alain et al., 2004; Szafranski et al., 2015a).”

    From Is It First the Egg or the Shrimp? – Diversity and Variation in Microbial Communities Colonizing Broods of the Vent Shrimp Rimicaris exoculata During Embryonic Development (2019) · cites this paper as López-García et al., 2003

    Split into 2 atoms — 0 confirmed, 2 partially supported, 0 disputed, 0 misattributed, 0 unverifiable. Expand the claim to see per-atom verdicts.

    Experimental — accuracy not yet measured

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Research works printed but not linked: 1

  1. Jobb, G. (2002) treefinder. [WWW document]. URL http://www.treefinder.de Kuenen, J.G., Robertson, L.A., and Tuovinen, O.H. (1992) The genera Thiobacillus, Thiomicrospira and Thiosphaera. In The Genera Thiobacillus, Thiomicrospira, and Thiosphaera. Balows, A., Trüper, H.G., Dworkin, M., Harder W., and Schleifer, K.H. (eds). Vol. III, New York: Springer-Verlag.

Cited by

Sorted by citations

Related

Authors
López‐García, P., Duperron, S., Philippot, P., Foriel, J., Susini, J., & Moreira, D.
Affiliations
  • Université Paris-Sud (FR)
  • Centre National de la Recherche Scientifique (FR)
  • European Synchrotron Radiation Facility (FR)
ORCID
Venue
Environmental Microbiology
Publisher
Wiley
Published
2003-08-14
Volume
5
Issue
10
Pages
961-976
ISSN
  • 1462-2912 (ISSN-L)
  • 1462-2920
DOI
10.1046/j.1462-2920.2003.00495.x
OpenAlex
W2139989406
Cited by
221
References
67
Citations by year
2026: 3 · 2025: 2 · 2023: 7 · 2022: 9 · 2021: 2 · 2020: 6 · 2019: 8 · 2018: 13 · 2017: 8 · 2016: 7 · 2015: 10 · 2014: 17 · 2013: 15 · 2012: 10
FWCI
10.82
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • Environmental Microbiology (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
  • DIAL (Catholic University of Leuven) (submittedVersion)
Funders
  • European Synchrotron Radiation Facility
  • Centre National de la Recherche Scientifique
Topics
  • Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
  • Physical Sciences › Environmental Science › Environmental Chemistry › Methane Hydrates and Related Phenomena
  • Physical Sciences › Earth and Planetary Sciences › Geochemistry and Petrology › Geochemistry and Elemental Analysis
Keywords
bacterial diversity, hydrothermal sediments, Epsilonproteobacteria, Mid-Atlantic Ridge, deep-sea hydrothermal vents, Gammaproteobacteria, Acidobacteria, sulfur reduction
MeSH terms
Atlantic Ocean, Hot Temperature, Iron, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S, Seawater, Spectrum Analysis, Genetic Variation, Water Microbiology, Likelihood Functions, Silicates, Geologic Sediments, Gammaproteobacteria, Epsilonproteobacteria

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review