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Cited by 107Ruminant Nutrition and Digestive PhysiologyAnaerobic Digestion and Biogas ProductionAnimal Nutrition and Physiology

Fibrolytic activities and cellulolytic bacterial community structure in the solid and liquid phases of rumen contents

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Michalet-Doreau, Brigitte, Fernandez, Isabelle, Peyron, C�line, Millet, Liliane, Fonty, G�rard·2001·Reproduction Nutrition Development
DOI 10.1051/rnd:2001122
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Abstract

Four sheep were fed an alfalfa hay diet.Rumen content samples were collected three hours after feeding in order to total microorganism population (TP), solid attached population (SAP) and solid attached firmly population (SAFP).Fibrolytic specific activities (xylanase, CMCase and β-glycosidases) were estimated by the amount of reducing sugars or p-nitrophenol released from the appropriate substrate.The distribution of the three main cellulolytic bacterial species (Fibrobacter succinogenes, Ruminococcus albus and Ruminococcus flavefaciens) was quantified by dot-blot hybridisation using specific 16S-rRNA-targeting probes.Specific activities of polysaccharidase enzymes were higher in SAP than in TP, and in SAFP than in SAP.The sum of RNA of the three cellulolytic bacterial species represented on average 9% of the total bacterial RNA, and increased after filtration.In all samples, the relative population size of F. succinogenes was higher than that of R. albus and of R. flavefaciens.These results demonstrate that the most active enzymes are secreted by the particle-associated microorganisms.The differences in composition of the microflora between the solid and liquid phase suggest that bacteria are not equally distributed throughout the rumen content: the cellulolytic species are present in a higher proportion in the solid phase of rumen contents. rumen content / fibrolytic activity / cellulolytic bacteria / oligonucleotide probeRésumé -Répartition de l'activité fibrolytique microbienne et des bactéries cellulolytiques entre phases solides et liquides du contenu ruminal.Quatre moutons, munis d'une canule du rumen, ont reçu un régime à base de foin.Des échantillons de contenus ruminaux étaient prélevés 3 h après la distribution du repas afin d'isoler la population totale de micro-organismes (TP), la population de microorganismes associés à la phase solide du contenu (SAP) et celle fermement associée à cette phase solide (SAFP).Les activités fibrolytiques spécifiques (xylanase, CMCase et β-glycosidases) étaient estimées

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  • The biofilm phenotype enables effective hydrolysis of plant material within the rumen by concentrating digestive enzymes within the EPS in proximity to the substrate.

    “The biofilm phenotype has many advantages, including the concentration of digestive enzymes within the EPS in proximity to the substrate, an arrangement that enables effective hydrolysis of plant material within the rumen (Minato et al., 1966; Wolin et al., 1997; Michalet-Doreau et al., 2001; Leng, 2014).”

    From Addressing global ruminant agricultural challenges through understanding the rumen microbiome: past, present, and future (2018) · cites this paper as Michalet-Doreau et al., 2001

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  1. Coleman G.S., Rumen entodiniomorphid pro- tozoa, in: Backer J.R., Taylor A.E.R. (Eds.), Methods of cultivating parasites in vitro, Aca- demic Press, London, UK, 1978, pp. 35-54.

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Authors
Michalet-Doreau, B., Fernandez, I., Peyron, C., Millet, L., & Fonty, G.
Affiliations
  • Unité Mixte de Recherche sur les Herbivores (FR)
  • Institut National de la Recherche Agronomique (FR)
Venue
Reproduction Nutrition Development
Publisher
EDP Sciences
Published
2001-03
Volume
41
Issue
2
Pages
187-194
ISSN
  • 0003-388X (ISSN-L)
  • 0926-5287
  • 1297-9708
DOI
10.1051/rnd:2001122
OpenAlex
W2121022323
Cited by
107
References
23
Citations by year
2025: 4 · 2024: 2 · 2023: 3 · 2022: 2 · 2021: 4 · 2020: 7 · 2019: 6 · 2018: 7 · 2017: 2 · 2016: 3 · 2015: 5 · 2014: 5 · 2013: 7 · 2012: 7
FWCI
6.96
Type
journal article
Language
en
Open access
Locations
  • annales de biologie animale biochimie biophysique (publishedVersion)
  • PubMed (publishedVersion)
  • Springer Link (Chiba Institute of Technology) (submittedVersion)
  • HAL (submittedVersion, other-oa)
  • HAL (submittedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
  • Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
Keywords
rumen contents, cellulolytic bacteria, bacterial community structure, solid phase, liquid phase, Fibrobacter succinogenes, Ruminococcus albus, Ruminococcus flavefaciens, xylanase, endoglucanase, β-glycosidase, sheep, rumen microbiota
MeSH terms
Medicago sativa, Animals, Bacterial Adhesion, Cellulase, Glycoside Hydrolases, Gram-Positive Cocci, RNA, Ribosomal, 16S, Rumen, Sheep, Xylosidases, Immunoblotting, Molecular Probe Techniques, Oligonucleotide Probes, Xylan Endo-1,3-beta-Xylosidase

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