Nitro-Oxidative Stress Biomarkers in Active and Inactive Men
Open accessAbstract
Oxidative stress markers are novel factors shown to be related to cardiovascular (CVD) risk. We examined the effects of long-term exercise, age, and their interaction on plasma oxidized LDL (ox-LDL), nitrotyrosine, and myeloperoxidase (MPO) levels, all biomarkers of oxidative stress, and determined their association with plasma nitric oxide (NOx) levels as an index of NO bioavailability. Older (62±2 yr) active men (n=12) who had exercised for >30 years and young (25±4 yr) active men (n=7) who had exercised for >3 years were age- and BMI-matched to older (n=11) and young (n=8) inactive men. Young subjects had lower plasma nitrotyrosine levels than older subjects (P=0.047). Young inactive subjects had higher ox-LDL levels than either the young active (P=0.042) or the older active (P=0.041) subjects. In addition, plasma oxidative stress levels, particularly ox-LDL, were correlated with various conventional plasma lipoprotein-lipid levels, and in older subjects were associated with Framingham risk score (r=0.49, P=0.015). We found no relationships between plasma oxidative stress markers and NOx levels. The findings suggest that a sedentary lifestyle may be associated with higher ox-LDL levels and that the levels of oxidative stress markers are related to levels of other conventional CVD risk factors and overall CVD risk.
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Sedentary lifestyles, characterised by inactive skeletal muscle, sarcopenia and adipose tissue accumulation, are associated with moderate-to-high levels of reactive oxygen species, which can deplete antioxidant defences resulting in oxidation of proteins, lipids and DNA.
“Sedentary lifestyles, characterised by inactive skeletal muscle, sarcopenia and adipose tissue accumulation, are associated with moderate-to-high levels of reactive oxygen species, which can deplete antioxidant defences resulting in oxidation of proteins, lipids and DNA (Bjork et al. 2012; Derbre et al. 2014; Gratas-Delamarche et al. 2014; Le Lay and Simard 2014).”
From Is immunosenescence influenced by our lifetime “dose” of exercise? (2016) · cites this paper as Bjork et al. 2012
Regular moderate-intensity exercise stimulates optimal reactive oxygen species production above resting values, but not to the levels associated with chronic sedentary behaviour.
“Regular moderate-intensity exercise stimulates optimal reactive oxygen species production above resting values, but not to the levels associated with chronic sedentary behaviour (Bjork et al. 2012; Derbre et al. 2014; Gratas-Delamarche et al. 2014; Le Lay and Simard 2014).”
From Is immunosenescence influenced by our lifetime “dose” of exercise? (2016) · cites this paper as Bjork et al. 2012
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Cites
Prediction of coronary heart disease using risk factor categories
Wilson, P. W. F., D’Agostino, R. B., Levy, D., Belanger, A. M., Silbershatz, H., & Kannel, W. B. (1998) · Circulation · doi:10.1161/01.cir.97.18.1837 · Cited by 9,581 · see citing works
Endothelial dysfunction in cardiovascular diseases: the role of oxidant stress
Cai, H., & Harrison, D. G. (2000) · Circulation Research · doi:10.1161/01.res.87.10.840 · Cited by 3,839 · see citing works
Oxidative Stress, Caloric Restriction, and Aging
Sohal, R. S., & Weindruch, R. H. (1996) · Science · doi:10.1126/science.273.5271.59 · Cited by 3,133 · see citing works
Role of Oxidative Modifications in Atherosclerosis
Stocker, R., & Keaney, J. F. (2004) · Physiological Reviews · doi:10.1152/physrev.00047.2003 · Cited by 2,611 · see citing works
Daugherty, A., Dunn, J. L., Rateri, D. L., & Heinecke, J. W. (1994) · Journal of Clinical Investigation · doi:10.1172/jci117342 · Cited by 1,269 · see citing works
Physical Activity and Reduced Risk of Cardiovascular Events: Potential Mediating Mechanisms
Mora, S., Cook, N., Buring, J. E., Ridker, P. M., & Lee, I.-M. (2007) · Circulation · doi:10.1161/circulationaha.107.729939 · Cited by 1,000 · see citing works
Association Between Myeloperoxidase Levels and Risk of Coronary Artery Disease
Zhang, R. (2001) · JAMA · doi:10.1001/jama.286.17.2136 · Cited by 919 · see citing works
DeSouza, C. A., Shapiro, L. F., Clevenger, C. M., Dinenno, F. A., Monahan, K. D., Tanaka, H., & Seals, D. R. (2000) · Circulation · doi:10.1161/01.cir.102.12.1351 · Cited by 896 · see citing works
Myeloperoxidase and Cardiovascular Disease
Nicholls, S. J., & Hazen, S. L. (2005) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.0000163262.83456.6d · Cited by 803 · see citing works
Holvoet, P., Vanhaecke, J., Janssens, S., de Werf, F. V., & Collen, D. (1998) · Circulation · doi:10.1161/01.cir.98.15.1487 · Cited by 673 · see citing works
Diet-induced atherosclerosis increases the release of nitrogen oxides from rabbit aorta.
Minor, R. L., Myers, P. R., Guerra, R., Bates, J. N., & Harrison, D. G. (1990) · Journal of Clinical Investigation · doi:10.1172/jci114949 · Cited by 517 · see citing works
Update – Ethical Standards in Sport and Exercise Science Research
Harriss, D., & Atkinson, G. (2011) · International Journal of Sports Medicine · doi:10.1055/s-0031-1287829 · Cited by 508 · see citing works
Association of Nitrotyrosine Levels With Cardiovascular Disease and Modulation by Statin Therapy
Shishehbor, M. H., Aviles, R. J., Brennan, M.-L., Fu, X., Goormastic, M., Pearce, G. L., Gokce, N., Keaney, J. F., Penn, M. S., Sprecher, D. L., et al. (2003) · JAMA · doi:10.1001/jama.289.13.1675 · Cited by 464 · see citing works
Leeuwenburgh, C., Hardy, M. M., Hazen, S. L., Wagner, P. D., Oh‐ishi, S., Steinbrecher, U. P., & Heinecke, J. W. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.3.1433 · Cited by 461 · see citing works
Meisinger, C., Baumert, J., Khuseyinova, N., Loewel, H., & Koenig, W. (2005) · Circulation · doi:10.1161/circulationaha.104.529297 · Cited by 434 · see citing works
Oxidative Stress and Antioxidants in Exercise
Leeuwenburgh, C., & Heinecke, J. W. (2001) · Current Medicinal Chemistry · doi:10.2174/0929867013372896 · Cited by 430 · see citing works
Oxidized LDL and HDL: antagonists in atherothrombosis
Mertens, A. C., & Holvoet, P. (2001) · The FASEB Journal · doi:10.1096/fj.01-0273rev · Cited by 410 · see citing works
Protein tyrosine nitration—Functional alteration or just a biomarker?
Souza, J. M., Peluffo, G., & Radí, R. (2008) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2008.04.010 · Cited by 394 · see citing works
Leeuwenburgh, C., RASMUSSEN, J. E., Hsu, F., Mueller, D. M., Pennathur, S., & Heinecke, J. W. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.6.3520 · Cited by 361 · see citing works
Hulthe, J., & Fagerberg, B. (2002) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.0000021150.63480.cd · Cited by 346 · see citing works
Maeda, S., Miyauchi, T., Kakiyama, T., Sugawara, J., Iemitsu, M., Irukayama‐Tomobe, Y., Murakami, H., Kumagai, Y., Kuno, S., & Matsuda, M. (2001) · Life Sciences · doi:10.1016/s0024-3205(01)01192-4 · Cited by 290 · see citing works
Elosua, R., Molina, L., Fito, M., Arquer, A., Sanchez-Quesada, J. L., Covas, M. I., Ordoñez-Llanos, J., & Marrugat, J. (2003) · Atherosclerosis · doi:10.1016/s0021-9150(03)00018-2 · Cited by 286 · see citing works
Plasma antioxidant status and cell injury after severe physical exercise
Chevion, S., Moran, D. S., Heled, Y., Shani, Y., Regev, G. J., Abbou, B., Berenshtein, E., Stadtman, E. R., & Epstein, Y. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0831097100 · Cited by 282 · see citing works
Consequences of Total and Subtotal Myeloperoxidase Deficiency: Risk or Benefit ?
Kutter, D., Devaquet, P., Vanderstocken, G., Paulus, J., Marchal, V., & Gothot, A. (2000) · Acta Haematologica · doi:10.1159/000041062 · Cited by 250 · see citing works
Oxidative Stress Responses in Older Men during Endurance Training and Detraining
Fatouros, I. G., Jamurtas, A. Z., Villiotou, V., POULIOPOULOU, S., Foteinakis, P. F., Taxildaris, K., & Deliconstantinos, G. (2004) · Medicine & Science in Sports & Exercise · doi:10.1249/01.mss.0000147632.17450.ff · Cited by 246 · see citing works
Mechanisms of oxidative stress and vascular dysfunction
Nedeljkovic, Z. S., Gokce, N., & Loscalzo, J. (2003) · Postgraduate Medical Journal · doi:10.1136/pmj.79.930.195 · Cited by 198 · see citing works
Byun, J., Mueller, D. M., Fabjan, J. S., & Heinecke, J. W. (1999) · FEBS Letters · doi:10.1016/s0014-5793(99)00893-5 · Cited by 125 · see citing works
Cornelissen, V., Arnout, J., Holvoet, P., & Fagard, R. H. (2009) · Journal of Hypertension · doi:10.1097/hjh.0b013e328322cf60 · Cited by 112 · see citing works
Futterman, L. G., & Lemberg, L. (1998) · American Journal of Critical Care · doi:10.4037/ajcc1998.7.3.240 · Cited by 86 · see citing works
Oxidized lipoproteins and nitric oxide
Jessup, W. (1996) · Current Opinion in Lipidology · doi:10.1097/00041433-199610000-00003 · Cited by 70 · see citing works
Penka, M., Rajković, M. G., Steiner, S., Strasser, B., Ziegler, S., Zorn, G., Maurer, G., Simeon-Rudolf, V., Wojta, J., Huber, K., et al. (2005) · Thrombosis and Haemostasis · doi:10.1160/th05-03-0158 · Cited by 69 · see citing works
Witkowski, S., Lockard, M. M., Jenkins, N. T., Obisesan, T. O., Spangenburg, E. E., & Hagberg, J. M. (2009) · Clinical Science · doi:10.1042/cs20090253 · Cited by 60 · see citing works
Self-citationJenkins, N. T., Witkowski, S., Spangenburg, E. E., & Hagberg, J. M. (2009) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.00347.2009 · Cited by 53 · see citing works
Self-citationOxidative stress and DNA damage in older adults that do exercises regularly
Mergener, M., Martins, M. R., Antunes, M. V., da Silva, C. C., Lazzaretti, C., Fontanive, T., Suyenaga, E. S., Ardenghi, P. G., Maluf, S. W., & Gamaro, G. D. (2009) · Clinical Biochemistry · doi:10.1016/j.clinbiochem.2009.08.001 · Cited by 37 · see citing works
Plasma fetuin-A concentrations in young and older high- and low-active men
Jenkins, N. T., McKenzie, J. A., Hagberg, J. M., & Witkowski, S. (2010) · Metabolism · doi:10.1016/j.metabol.2010.01.026 · Cited by 33 · see citing works
Self-citationBrinkley, T. E., Fenty-Stewart, N., Park, J., Brown, M. D., & Hagberg, J. M. (2009) · Nitric Oxide · doi:10.1016/j.niox.2009.10.001 · Cited by 15 · see citing works
Self-citation
Flagged first, then by citations
Research works printed but not linked: 3
- Napoli C , Williams-Ignarro S , De Nigris F , Lerman L O , Rossi L , Guarino C , Mansueto G , Di Tuoro F , Pignalosa O , De Rosa G , Sica V , Ignarro L J . Long- term combined benefi cial eff ects of physical training and metabolic treatment on atherosclerosis in hypercholesterolemic mice . Proc Natl Acad Sci U S A 2004 ; 101 : 8797 -8802
- Palinski W , Rosenfeld M E , Yla-Herttuala S , Gurtner G C , Socher S S , Butler S W , Parthasarathy S , Carew T E , Steinberg D , Witztum J L . Low density lipoprotein undergoes oxidative modifi cation in vivo . Proc Natl Acad Sci U S A 1989 ; 86 : 1372 -1376
- Stocker R , Keaney J F Jr . Role of oxidative modifi cations in atheroscle- rosis . Physiol Rev 2004 ; 84 : 1381 -1478
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Lessiani, G., Santilli, F., Boccatonda, A., Iodice, P., Liani, R., Tripaldi, R., Saggini, R., & Davı̀, G. (2015) · Vascular Pharmacology · doi:10.1016/j.vph.2015.05.017 · Cited by 65 · see citing works
Lemke, D., Pledl, H.-W., Zorn, M., Jugold, M., Green, E., Blaes, J., Löw, S., Hertenstein, A., Ott, M., Sahm, F., et al. (2016) · Oncotarget · doi:10.18632/oncotarget.10723 · Cited by 40 · see citing works
Zembroń-Łacny, A., Dziubek, W., Rynkiewicz, M., Morawin, B., & Woźniewski, M. (2016) · Brazilian Journal of Medical and Biological Research · doi:10.1590/1414-431x20165253 · Cited by 36 · see citing works
Daniele, A., Lucas, S. J. E., & Rendeiro, C. (2022) · Frontiers in Physiology · doi:10.3389/fphys.2022.998380 · Cited by 35 · see citing works
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- Authors
- Bjork, L., Jenkins, N., Witkowski, S., & Hagberg, J.
- Affiliations
- University of Maryland, College Park (US)
- Venue
- International Journal of Sports Medicine
- Publisher
- Georg Thieme Verlag KG
- Published
- 2012-02-29
- Volume
- 33
- Issue
- 04
- Pages
- 279-284
- ISSN
- 0172-4622 (ISSN-L)
- 1439-3964
- OpenAlex
- W2047038980
- Cited by
- 27
- References
- 36
- Citations by year
- 2025: 1 · 2023: 1 · 2022: 2 · 2021: 4 · 2020: 3 · 2019: 2 · 2018: 2 · 2017: 1 · 2016: 3 · 2015: 4 · 2014: 1 · 2013: 1 · 2012: 2
- FWCI
- 2.70
- Type
- journal article
- Language
- en
- Open access
- green
- works.bepress.com
- Locations
- International Journal of Sports Medicine (publishedVersion)
- PubMed (publishedVersion)
- ScholarWorks@UMassAmherst (University of Massachusetts Amherst) (submittedVersion)
- Funders
- National Institute on Aging (T32-AG-00268, T32AG000268)
- Topics
- Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Keywords
- oxidative stress biomarkers, nitrotyrosine, oxidized LDL, myeloperoxidase, nitric oxide bioavailability, long-term exercise, aging, sedentary lifestyle, cardiovascular risk, Framingham risk score, men
- MeSH terms
- Adult, Age Factors, Humans, Lipoproteins, LDL, Male, Middle Aged, Peroxidase, Nitric Oxide, Tyrosine, Biomarkers, Exercise, Oxidative Stress, Sedentary Behavior
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