Studies on the Effects of fpf1 Gene on Artemisia annua Flowering Time and on the Linkage between Flowering and Artemisinin Biosynthesis
Abstract
The flowering promoting factor1 ( fpf1) from Arabidopsis thaliana was transferred into Artemisia annua L. via Agrobacterium tumefaciens. The fpf1 gene was firstly inserted in the binary vector pBI121 under the control of CaMV 35S promoter to construct the plant expression vector pBIfpf1, then leaf explants of A. annua were infected with A. tumefaciens LBA4404 containing pBIfpf1, and induced shoots. Transgenic plants were obtained through the selection with kanamycin. PCR, PCR-Southern and Southern blot analyses confirmed that the foreign fpf1 gene had been integrated into the A. annua genome. RT-PCR and RT-PCR-Southern analyses suggested that the foreign fpf1 gene had expressed at the transcriptional level. Under short-day conditions, the flowering time of fpf1 transgenic plants was about 20 days earlier than the non-transformed plants; however, no significant differences were detected in artemisinin content between the flowering transgenic plants and the non-flowering non-transgenic plants. These results showed that flowering is not a necessary factor for increasing the artemisinin content, furthermore, there may be no direct linkage between flowering and artemisinin biosynthesis.
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Several authors have reported that the artemisinin content is highest just before flowering.
“Several authors have reported that the artemisinin content is highest just before flowering [326,327,328]”
From The Biosynthesis of Artemisinin (Qinghaosu) and the Phytochemistry of Artemisia annua L. (Qinghao) (2010) · cites this paper as [327]
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The Biosynthesis of Artemisinin (Qinghaosu) and the Phytochemistry of Artemisia annua L. (Qinghao)
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“This now appears unlikely as two studies that induced premature flowering using the early flowering gene CONSTANS (Wang et al. 2004) or the flowering promoting factor1 (Wang et al.”
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- Authors
- Wang, H., Ge, L., Ye, H., Chong, K., Liu, B., & Li, G.
- Affiliations
- Chinese Academy of Sciences (CN)
- Venue
- Planta Medica
- Publisher
- Georg Thieme Verlag KG
- Published
- 2004-04
- Volume
- 70
- Issue
- 4
- Pages
- 347-352
- ISSN
- 0032-0943 (ISSN-L)
- 1439-0221
- OpenAlex
- W2404204400
- Cited by
- 39
- References
- 0
- Citations by year
- 2026: 1 · 2024: 1 · 2023: 1 · 2022: 1 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 1 · 2015: 3 · 2014: 2 · 2013: 1 · 2012: 4
- FWCI
- 0.95
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Planta Medica (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Keywords
- Artemisia annua, flowering time, artemisinin biosynthesis, transgenic plants, Agrobacterium-mediated transformation, short-day conditions, gene expression, artemisinin content
- MeSH terms
- Humans, Light, Phytotherapy, Plant Proteins, Sesquiterpenes, Polymerase Chain Reaction, DNA Primers, Plant Leaves, Reverse Transcriptase Polymerase Chain Reaction, Arabidopsis Proteins, Plants, Genetically Modified, Artemisia annua, Flowers, Artemisinins
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