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Cited by 54Spaceflight effects on biologySepsis Diagnosis and TreatmentCardiovascular Syncope and Autonomic Disorders

Effects of the hindlimb-unloading model of spaceflight conditions on resistance of mice to infection with Klebsiella pneumoniae

Belay, Tesfaye, Aviles, Hernan, Vance, Monique, Fountain, Kimberly, Sonnenfeld, Gerald·2002·Journal of Allergy and Clinical Immunology
DOI 10.1067/mai.2002.126459
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Abstract

Background It has been well documented in several studies that many immunologic parameters are altered in experimental animals and human subjects who have flown in space. However, it is not fully known whether these immunologic changes could result in increased susceptibility to infection. Hindlimb (antiorthostatic) unloading of rodents has been used successfully to simulate some of the effects of spaceflight on physiologic systems.Objective The objective of this study was to determine the effect of hindlimb unloading on the outcome of Klebsiella pneumoniae infection in mice.Methods Hindlimb-unloaded, hindlimb-restrained, and control mice were intraperitoneally infected with one 50% lethal dose of K pneumoniae 2 days after suspension. Mortality and bacterial load in several organs were compared among the groups.Results Unloaded mice showed significantly increased mortality and reduced mean time to death compared with that seen in the control groups. Kinetics of bacterial growth with smaller infective doses revealed that control mice were able to clear bacteria from the organs after 30 hours. In contrast, unloaded mice had continued bacterial growth at the same time point.Conclusion The results of this study suggest that hindlimb unloading might enhance the dissemination of K pneumoniae, leading to increased mortality. The complex physiologic changes observed during hindlimb unloading, including stress, have a key role in the pathophysiology of this infection.

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  • Animal models of space flight have demonstrated a reduced ability to clear infections by Klebsiella pneumoniae and Pseudomonas aeruginosa.

    “Animal models of space flight have demonstrated a reduced ability to clear infections by Klebsiella pneumoniae [24] and Pseudomonas aeruginosa [25].”

    From Hindlimb suspension and spe-like radiation impairs clearance of bacterial infections (2014) · cites this paper as [24]

  • Hindlimb suspended mice given IP Klebsiella pneumoniae had an increase in mortality and bacterial organ loads.

    “Hindlimb suspended mice given IP Klebsiella pneumoniae similarly had an increase in mortality and bacterial organ loads [24].”

    From Hindlimb suspension and spe-like radiation impairs clearance of bacterial infections (2014) · cites this paper as [24]

  • Studies have indicated a reduction in the ability of hindlimb unloaded mice to control bacterial infections.

    “Studies have indicated a reduction in the ability of hindlimb unloaded mice to control bacterial infections.”

    From Hindlimb suspension and spe-like radiation impairs clearance of bacterial infections (2014) · cites this paper as [24]

  • The studies of systemic Klebsiella pneumoniae and Pseudomonas aeruginosa gave mice an LD50 of bacteria, which allowed small changes in immune function to result in significant increases in mortality above the expected 50%.

    “The studies of systemic Klebsiella pneumoniae and Pseudomonas aeruginosa gave mice an LD50 of bacteria [24], [25], which allowed small changes in immune function to result in significant increases in mortality above the expected 50%.”

    From Hindlimb suspension and spe-like radiation impairs clearance of bacterial infections (2014) · cites this paper as [24]

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Authors
Belay, T., Aviles, H., Vance, M., Fountain, K., & Sonnenfeld, G.
Affiliations
Morehouse School of Medicine (US)
ORCID
Belay, Tesfaye
Venue
Journal of Allergy and Clinical Immunology
Publisher
Elsevier BV
Published
2002-08
Volume
110
Issue
2
Pages
262-268
ISSN
0091-6749 (ISSN-L)
DOI
10.1067/mai.2002.126459
OpenAlex
W1970221893
Cited by
54
References
42
Citations by year
2026: 2 · 2025: 2 · 2024: 1 · 2022: 1 · 2021: 2 · 2020: 3 · 2019: 3 · 2018: 2 · 2017: 1 · 2016: 2 · 2015: 3 · 2014: 4 · 2013: 3 · 2012: 2
FWCI
2.40
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Journal of Allergy and Clinical Immunology (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Center for Research Resources (5G12 RR/AI 03034)
Topics
  • Health Sciences › Medicine › Physiology › Spaceflight effects on biology
  • Health Sciences › Medicine › Epidemiology › Sepsis Diagnosis and Treatment
  • Health Sciences › Medicine › Surgery › Cardiovascular Syncope and Autonomic Disorders
Keywords
hindlimb unloading, Klebsiella pneumoniae, infection susceptibility, spaceflight immunology, bacterial dissemination, bacterial clearance, mortality, stress, mice
MeSH terms
Animals, Disease Models, Animal, Female, Hindlimb, Immunity, Innate, Kidney, Klebsiella Infections, Klebsiella pneumoniae, Liver, Lung, Space Flight, Spleen, Time Factors, Survival Rate, Hindlimb Suspension, Mice

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