Mimicking live flavivirus immunization with a noninfectious RNA vaccine
Open accessAbstract
Flaviviruses are human pathogens of world-wide medical importance. They have recently received much additional attention because of their spread to new regions (such as West Nile virus to North America), highlighting their potential as newly emerging disease agents. Using tick-borne encephalitis virus, we have developed and evaluated in mice a new genetic vaccine based on self-replicating but noninfectious RNA. This RNA contains all of the necessary genetic information for establishing its replication machinery in the host cell, thus mimicking a natural infection. However, genetic modifications in the region encoding the capsid protein simultaneously prevent the assembly of infectious virus particles and promote the secretion of noninfectious subviral particles that elicit neutralizing antibodies. These characteristics demonstrate that a new generation of flavivirus vaccines can be designed that stimulate the same spectrum of innate and specific immune responses as a live vaccine but have the safety features of an inactivated vaccine.
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Recombinant viral vector technologies have the advantage of efficient delivery of the nucleic acid payload, but their utility is often hampered by antivector immunity, production limitations, and safety concerns.
“Recombinant viral vector technologies have the advantage of efficient delivery of the nucleic acid payload, but their utility is often hampered by antivector immunity, production limitations, and safety concerns (3,4).”
From Nonviral delivery of self-amplifying RNA vaccines (2012) · cites this paper as [3]
There are limited published data on the in vivo delivery of self-amplifying RNA using nonviral delivery strategies, and none has taken advantage of the recently developed, clinically suitable delivery systems for siRNA.
“There are limited published data on the in vivo delivery of self-amplifying RNA using nonviral delivery strategies (3,14,18), and none has taken advantage of the recently developed, clinically suitable delivery systems for siRNA (19,20).”
From Nonviral delivery of self-amplifying RNA vaccines (2012) · cites this paper as [3]
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- Authors
- Kofler, R. M., Aberle, J. H., Aberle, S. W., Allison, S. L., Heinz, F. X., & Mandl, C. W.
- Affiliations
- Medical University of Vienna (AT)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2004-02-09
- Volume
- 101
- Issue
- 7
- Pages
- 1951-1956
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC357033
- OpenAlex
- W1981821156
- Cited by
- 116
- References
- 36
- Citations by year
- 2025: 1 · 2024: 5 · 2023: 10 · 2022: 5 · 2021: 4 · 2020: 3 · 2019: 2 · 2018: 9 · 2017: 6 · 2016: 5 · 2015: 2 · 2014: 9 · 2013: 8 · 2012: 2
- FWCI
- 6.45
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Mosquito-borne diseases and control
- Health Sciences › Medicine › Infectious Diseases › Viral Infections and Vectors
- Health Sciences › Medicine › Epidemiology › Influenza Virus Research Studies
- Keywords
- flavivirus vaccines, tick-borne encephalitis virus, self-replicating RNA, subviral particles, neutralizing antibodies, innate immune response, emerging viral diseases, West Nile virus
- MeSH terms
- Animals, Antibodies, Viral, Cell Line, Flavivirus, Cricetinae, RNA, Viral, Vaccination, Vaccines, Attenuated, Viral Vaccines, Virion, Gene Deletion, Flavivirus Infections, Capsid Proteins, Mice
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