The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.
Brain and muscle Arnt-like protein-1 (BMAL1), a component of the molecular clock, regulates adipogenesis
Open accessAbstract
Brain and muscle Arnt-like protein-1 (BMAL1; also known as MOP3 or Arnt3) is a transcription factor known to regulate circadian rhythm. Here, we established its involvement in the control of adipogenesis and lipid metabolism activity in mature adipocytes. During adipose differentiation in 3T3-L1 cells, the level of BMAL1 mRNA began to increase 4 days after induction and was highly expressed in differentiated cells. In white adipose tissues isolated from C57BL/6J mice, BMAL1 was predominantly expressed in a fraction containing adipocytes, as compared with the stromal-vascular fraction. BMAL1 knockout mice embryonic fibroblast cells failed to be differentiated into adipocytes. Importantly, adding BMAL1 back by adenovirus gene transfer restored the ability of BMAL1 knockout mice embryonic fibroblast cells to differentiate. Knock-down of BMAL1 expression in 3T3-L1 cells by an RNA interference technique allowed the cells to accumulate only minimum amounts of lipid droplets in the cells. Adenovirus-mediated expression of BMAL1 in 3T3-L1 adipocytes resulted in induction of several factors involved in lipogenesis. The promoter activity of these genes was stimulated in a BMAL1-dependent manner. Interestingly, expression of these factors showed clear circadian rhythm in mice adipose tissue. Furthermore, overexpression of BMAL1 in adipocytes increased lipid synthesis activity. These results indicate that BMAL1, a master regulator of circadian rhythm, also plays important roles in the regulation of adipose differentiation and lipogenesis in mature adipocytes.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Knockout of Bmal1 alone in C57BL/6J mice showed that isolated mouse embryonic fibroblast (MEF) cells could not successfully differentiate into adipocytes (Shimba et al., 2005).”
From The impact of the circadian timing system on cardiovascular and metabolic function (2012) · cites this paper as Shimba et al., 2005
In the citing paper
“The presence of high Bmal1 mRNA levels in adipocytes increased lipogenesis activity, further suggesting that Bmal1 is involved in regulating lipid metabolism.”
From The impact of the circadian timing system on cardiovascular and metabolic function (2012) · cites this paper as Shimba et al., 2005
In the citing paper
“Further, restoration of Bmal1 function by adenovirus gene transfer into the initial Bmal1-deficient mice restored adipocyte differentiation and lipid accumulation in the MEF cells.”
From The impact of the circadian timing system on cardiovascular and metabolic function (2012) · cites this paper as Shimba et al., 2005
Citations
Cites
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302
Coordination of circadian timing in mammals
Reppert, S. M., & Weaver, D. R. (2002) · Nature · doi:10.1038/nature00965 · Cited by 4,379
Molecular Bases for Circadian Clocks
Dunlap, J. C. (1999) · Cell · doi:10.1016/s0092-8674(00)80566-8 · Cited by 2,900
Obesity and Metabolic Syndrome in Circadian Clock Mutant Mice
Turek, F. W., Joshu, C., Kohsaka, A., Lin, E., Ivanova, G., McDearmon, E., Laposky, A., Losee-Olson, S., Easton, A., Jensen, D. R., et al. (2005) · Science · doi:10.1126/science.1108750 · Cited by 2,485
Obesity and the regulation of energy balance
Spiegelman, B. M., & Flier, J. S. (2001) · Cell · doi:10.1016/s0092-8674(01)00240-9 · Cited by 2,398
Mop3 is an essential component of the master circadian pacemaker in mammals.
Bunger, M. K., Wilsbacher, L. D., Moran, S. M., Clendenin, C., Radcliffe, L. A., Hogenesch, J. B., Simon, M. C., Takahashi, J. S., & Bradfield, C. A. (2000) · Cell · doi:10.1016/s0092-8674(00)00205-1 · Cited by 1,621
Adipose Tissue as an Endocrine Organ
Ahima, R. S., & Flier, J. S. (2000) · Trends in Endocrinology & Metabolism · doi:10.1016/s1043-2760(00)00301-5 · Cited by 1,608
Time zones: a comparative genetics of circadian clocks
Young, M. W., & Kay, S. A. (2001) · Nature Reviews Genetics · doi:10.1038/35088576 · Cited by 1,159
Rudic, R. D., McNamara, P., Curtis, A.-M., Boston, R. C., Panda, S., Hogenesch, J. B., & FitzGerald, G. A. (2004) · PLoS Biology · doi:10.1371/journal.pbio.0020377 · Cited by 1,059
Regulation of clock and NPAS2 DNA binding by the redox state of NAD cofactors
Rutter, J., Reick, M., Wu, L. C., & McKnight, S. L. (2001) · Science · doi:10.1126/science.1060698 · Cited by 1,059
ADD1/SREBP1 promotes adipocyte differentiation and gene expression linked to fatty acid metabolism.Preprint
Kim, J. B., & Spiegelman, B. M. (1996) · Genes & Development · doi:10.1101/gad.10.9.1096 · Cited by 1,026
Mammalian circadian biology: elucidating genome-wide levels of temporal organization
Lowrey, P. L., & Takahashi, J. S. (2004) · Annual Review of Genomics and Human Genetics · doi:10.1146/annurev.genom.5.061903.175925 · Cited by 976
Shimano, H., Horton, J. D., Hammer, R. E., Shimomura, I., Brown, M. S., & Goldstein, J. L. (1996) · Journal of Clinical Investigation · doi:10.1172/jci118951 · Cited by 817
Hogenesch, J. B., Gu, Y.-Z., Jain, S. R., & Bradfield, C. A. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.10.5474 · Cited by 781
Shimomura, I., Shimano, H., Horton, J. D., Goldstein, J. L., & Brown, M. S. (1997) · Journal of Clinical Investigation · doi:10.1172/jci119247 · Cited by 766
ADD1/SREBP1 activates PPARγ through the production of endogenous ligand
Kim, J. B., Wright, H. M., Wright, M., & Spiegelman, B. M. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.8.4333 · Cited by 670
Magaña, M., & Osborne, T. F. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.51.32689 · Cited by 336
Fontaine, C., Dubois, G., Duguay, Y., Helledie, T., Vu‐Dac, N., Gervois, P. P., Soncin, F., Mandrup, S., Fruchart, J., Fruchart‐Najib, J., et al. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m304664200 · Cited by 248
Kondratov, R. V., Chernov, M. V., Kondratova, A. A., Gorbacheva, V., Gudkov, A. V., & Antoch, M. P. (2003) · Genes & Development · doi:10.1101/gad.1099503 · Cited by 240
Horton, J. D., Shimomura, I., Ikemoto, S., Bashmakov, Y. K., & Hammer, R. E. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m306540200 · Cited by 229
Ericsson, J., Jackson, S. M., Kim, J. B., Spiegelman, B. M., & Edwards, P. A. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.11.7298 · Cited by 227
Ikeda, M., & Nomura, M. (1997) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1997.6371 · Cited by 220
Progressive arthropathy in mice with a targeted disruption of theMop3/Bmal-1 locus
Bunger, M. K., Walisser, J. A., Sullivan, R., Manley, P. A., Moran, S. M., Kalscheur, V. L., Colman, R. J., & Bradfield, C. A. (2005) · genesis · doi:10.1002/gene.20102 · Cited by 207
Chawla, A., & Lazar, M. A. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)85415-7 · Cited by 200
The orphan receptor Rev-erb α gene is a target of the circadian clock pacemaker
Triqueneaux, G., Thénot, S., Kakizawa, T., Antoch, M. P., Safi, R., Takahashi, J. S., Delaunay, F., & Laudet, V. (2004) · Journal of Molecular Endocrinology · doi:10.1677/jme.1.01554 · Cited by 174
Circadian variations of blood sugar and plasma insulin levels in man
Malherbe, C., Marc De Gasparo, B., Hertogh, R. D., & Hoem, J. J. (1969) · Diabetologia · doi:10.1007/bf00427978 · Cited by 167
Takahata, S., Sogawa, K., Kobayashi, A., Ema, M., Mimura, J., Ozaki, N., & Fujii‐Kuriyama, Y. (1998) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1998.9012 · Cited by 153
Schlierf, G., & Dorow, E. (1973) · Journal of Clinical Investigation · doi:10.1172/jci107235 · Cited by 148
Transcription Regulation within the Circadian Clock: The E-box and Beyond
Hardin, P. E. (2004) · Journal of Biological Rhythms · doi:10.1177/0748730404268052 · Cited by 148
Yamamoto, T., Shimano, H., Nakagawa, Y., Ide, T., Yahagi, N., Matsuzaka, T., Nakakuki, M., Takahashi, A., Suzuki, H., Sone, H., et al. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m310333200 · Cited by 143
Bécard, D., Hainault, I., Azzout‐Marniche, D., Bertry-Coussot, L., Ferré, P., & Foufelle, F. (2001) · Diabetes · doi:10.2337/diabetes.50.11.2425 · Cited by 133
Magana, M. M., Lin, S. S., Dooley, K. A., & Osborne, T. F. (1997) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)37181-9 · Cited by 124
Chakravarty, K., Leahy, P. S., Bécard, D., Hakimi, P., Foretz, M., Ferré, P., Foufelle, F., & Hanson, R. W. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m103310200 · Cited by 92
Circadian Periodicity in Some Physicochemical Parameters of Circulating Blood
Seaman, G. V. F., ENGEL, R., SWANK, R. L., & HISSEN, W. (1965) · Nature · doi:10.1038/207833a0 · Cited by 90
Chakravarty, K., Wu, S.-Y., Chiang, C.-M., Samols, D. R., & Hanson, R. W. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m309905200 · Cited by 70
Takagi, S., Tojo, H., Tomita, S., Sano, S., Itami, S., Hara, M., Inoue, S., Horie, K., Kondoh, G., Hosokawa, K., et al. (2003) · Journal of Clinical Investigation · doi:10.1172/jci200318513 · Cited by 60
Regulation of adiponectin receptor R1 and R2 gene expression in adipocytes of C57BL/6 mice
Blüher, M., Faßhauer, M., Kralisch, S., Schön, M. R., Krohn, K., & Paschke, R. (2005) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2005.02.081 · Cited by 55
Circadian Variation of the Serum Glucose and Immunoreactive Insulin Levels
Gagliardino, J. J., & HERNÉNDEZ, R. E. (1971) · Endocrinology · doi:10.1210/endo-88-6-1529 · Cited by 49
Characteristics of Circadian Gene Expressions in Mice White Adipose Tissue and 3T3-L1 Adipocytes
Aoyagi, T., Shimba, S., & Tezuka, M. (2005) · Journal of Health Science · doi:10.1248/jhs.51.21 · Cited by 37
Self-citation
Flagged first, then by citations
Research works printed but not linked: 2
- Gagliardino, J. J. & Hernandez, R. E. (1971) Endocrinology 88, 1532–1534.5572981
- Rudic, R. D., McNamara, P., Curtis, A.-M., Boston, R. C., Panda, S., Hogenesch, J. B. & FitGerald, G. A. (2004) PLoS Biol. 2, 1893–1899.
Cited by
Sorted by citations
Adipocyte differentiation from the inside out
Rosen, E. D., & MacDougald, O. A. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2066 · Cited by 2,607
Transcriptional control of adipocyte formation
Farmer, S. R. (2006) · Cell Metabolism · doi:10.1016/j.cmet.2006.07.001 · Cited by 1,836
Molecular components of the mammalian circadian clock
Ko, C. H., & Takahashi, J. S. (2006) · Human Molecular Genetics · doi:10.1093/hmg/ddl207 · Cited by 1,725
The genetics of mammalian circadian order and disorder: implications for physiology and disease
Takahashi, J. S., Hong, H.-K., Ko, C. H., & McDearmon, E. L. (2008) · Nature Reviews Genetics · doi:10.1038/nrg2430 · Cited by 1,611
High-Fat Diet Disrupts Behavioral and Molecular Circadian Rhythms in Mice
Kohsaka, A., Laposky, A. D., Ramsey, K. M., Estrada, C., Joshu, C., Kobayashi, Y., Turek, F. W., & Bass, J. (2007) · Cell Metabolism · doi:10.1016/j.cmet.2007.09.006 · Cited by 1,528
Metadata
- Authors
- Shimba, S., Ishii, N., Ohta, Y., Ohno, T., Watabe, Y., Hayashi, M., Wada, T., Aoyagi, T., & Tezuka, M.
- Affiliations
- Nihon University (JP)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2005-08-10
- Volume
- 102
- Issue
- 34
- Pages
- 12071-12076
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC1189312
- OpenAlex
- W2047902467
- Cited by
- 536
- References
- 39
- Citations by year
- 2026: 15 · 2025: 11 · 2024: 21 · 2023: 21 · 2022: 22 · 2021: 28 · 2020: 19 · 2019: 16 · 2018: 22 · 2017: 24 · 2016: 19 · 2015: 24 · 2014: 33 · 2013: 40 · 2012: 32
- FWCI
- 9.44
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Nihon University
- Ono Medical Research Foundation
- Topics
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
- Health Sciences › Medicine › Physiology (medical) › Adipose Tissue and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
- Keywords
- Bmal1, circadian clock, adipogenesis, lipogenesis, lipid metabolism, 3T3-L1 cells, adipose tissue, circadian rhythm, transcription factors, adipocytes, lipid droplets, gene knockdown, adenoviral gene transfer
- MeSH terms
- Adenoviridae, Adipose Tissue, Animals, Brain, Circadian Rhythm, Gene Expression Regulation, Genetic Vectors, Male, Mice, Inbred C57BL, Immunoblotting, 3T3 Cells, DNA Primers, Gene Transfer Techniques, Muscle, Skeletal, Reverse Transcriptase Polymerase Chain Reaction, RNA Interference, Chromatin Immunoprecipitation, Adipogenesis, Lipid Metabolism, Mice, Basic Helix-Loop-Helix Proteins, Basic Helix-Loop-Helix Transcription Factors, ARNTL Transcription Factors
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review